Determination of ornithine conjugates of some carboxylic acids in birds by high-performance liquid chromatography.


:A high-performance liquid chromatographic (HPLC) method for the determination of ornithine conjugation of some carboxylic acids in vitro has been developed. The ornithine conjugates of benzoic acid, p-nitrobenzoic acid, furancarboxylic acid and phenylacetic acid in an incubation mixture with kidney mitochondria were well separated on a reversed-phase C18 column using a mixture of 10 mM ammonium acetate buffer and methanol as the mobile phase. In addition, by varying the pH of the mobile phase and utilizing the absorption wavelengths (nm) of the conjugates it was possible to resolve and specifically detect each conjugate. The calibration curves were linear in the range of 0.2-16 micrograms/ml for all compounds and the detection limits were about 50 ng/ml except for the ornithine conjugate of phenyl acetic acid (S/N = 2). The ornithine conjugation of some carboxylic acids with chicken kidney mitochondria were determined by this assay method. The activity of ornithine conjugation of benzoic acid, furancarboxylic acid, p-nitrobenzoic acid and phenylacetic acid were 14.5, 5.5, 0.5 and 6.9 nmol/mg of protein, respectively. Moreover, the ornithine conjugation and the glycine conjugation of benzoic acid were examined in birds and rodents. The ornithine conjugation was observed only in chicken (14.5 nmol/mg of protein) and mallard (0.99 nmol/mg of protein).


Igarashi K,Suzuki R,Kasuya F,Fukui M




Has Abstract


1992-08-01 00:00:00












  • Lignans from Santalum album and their cytotoxic activities.

    abstract::A new neolignan, (7R,8R)-5-O-demethylbilagrewin (1), together with four known lignans (2-5), were isolated from the heartwood of Santalum album (Santalaceae). The structure of 1 was determined by analysis of extensive spectroscopic data. The isolated compounds and derivatives were evaluated for their cytotoxic activit...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Matsuo Y,Mimaki Y

    更新日期:2010-04-01 00:00:00

  • Preparation of Cocoa Powder-Containing Orally Disintegrating Tablets of Rebamipide (Rebamipide Chocolet) and Evaluation of Their Clinical Palatability.

    abstract::Orally disintegrating tablets (ODTs), which are administered without water, are beneficial for elderly patients and patients with dysphagia. Masking the unpleasant taste of a drug is an important factor associated with adherence of patients consuming ODTs. We prepared cocoa powder-containing ODTs of bitter-tasting reb...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Takano H,Uchida S,Kashiwagura Y,Tanaka S,Hakamata A,Odagiri K,Inui N,Watanabe H,Namiki N

    更新日期:2019-01-01 00:00:00

  • Facile synthesis and quantitative structure-activity relationship study of antitumor active 2-(4-oxo-thiazolidin-2-ylidene)-3-oxo-propionitriles.

    abstract::2-(5-Arylidene-4-oxo-3-phenyl-thiazolidin-2-ylidene)-3-oxo-propionitriles 4a-j were prepared via condensation of aromatic aldehydes with 4-thiazolidinones 3a,b. The latter was obtained via electrophilic attack of phenylisothiocyanate on 3-oxo-propionitriles 1a,b followed by reaction with chloroacetyl chloride under ba...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Hanna MM,George RF

    更新日期:2012-01-01 00:00:00

  • Preparation on oligostilbenes of isorhapontigenin by oxidative coupling reaction.

    abstract::Four new compounds 1-4 were obtained from an oxidative coupling reaction of (E)-isorhapontigenin using FeCl(3) as oxidant. Their structures and stereochemistry were determined on the basis of spectroscopic evidence [UV, IR, MS, (1)H-, (13)C-NMR, NOE and 2D NMR], and their possible formation mechanisms were also discus...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Yao CS,Zhou LX,Lin M

    更新日期:2004-02-01 00:00:00

  • Chemistry of renieramycins. Part 4.synthesis of a simple natural marine product, 6-hydroxy-7-methoxyisoquinolinemethanol.

    abstract::6-Hydroxy-7-methoxyisoquinolinemethanol (15) and mimosamycin (1) were recently isolated from a marine sponge, Haliclona sp. The former was prepared in ten steps from vanillin (22) in 26% overall yield using an isopropyl for phenol protection. ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Saito N,Tanaka C,Satomi T,Oyama C,Kubo A

    更新日期:2004-02-01 00:00:00

  • Synthesis and antitumor activity of novel pyrimidinyl pyrazole derivatives. II. Optimization of the phenylpiperazine moiety of 1-[5-methyl-1-(2-pyrimidinyl)-4-pyrazolyl]-3-phenylpiperazinyl-1-trans-propenes.

    abstract::A series of novel 3-substituted-1-[5-methyl-1-(2-pyrimidinyl)-4-pyrazolyl]-1-trans-propenes in order to improve the in vitro and in vivo activity of our prototype 3-[4-(3-chlorophenyl)-1-piperazinyl]-1-[5-methyl-1-(2-pyrimidinyl)-4-pyrazolyl]-1-trans-propene (2) were synthesized and evaluated by assays of growth inhib...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Naito H,Ohsuki S,Sugimori M,Atsumi R,Minami M,Nakamura Y,Ishii M,Hirotani K,Kumazawa E,Ejima A

    更新日期:2002-04-01 00:00:00

  • Further studies on new furostanol saponins from the bulbs of Allium macrostemon.

    abstract::Further studies by means of preparative HPLC led to the isolation of two new furostanol saponins, macrostemonoside J (1) and L (3), along with an artifact, macrostemonoside K (2) from the bulbs of Allium macrostemon. On the basis of chemical evidence and spectral analysis (1H-, 13C-NMR and FAB-MS), the structure of 1 ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Peng J,Yao X,Okada Y,Okuyama T

    更新日期:1994-10-01 00:00:00

  • Functional analysis of the iron(II) etiocorrphycene incorporated in the myoglobin heme pocket.

    abstract::The iron(III) complex of 2,7,12,17-tetraethyl-3,6,11,18-tetra-methylcorrphycene, an isomeric heme, was complexed with apomyoglobin to examine the ligand binding ability of the novel macrocycle under physiological conditions. The reconstituted holoprotein was found to be functionally active at pH 7.4 and 20 degrees C a...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Neya S,Nakamura M,Imai K,Funasaki N

    更新日期:2001-03-01 00:00:00

  • Syntheses of cerulenin and its analogs. II. Synthesis and biological activity of dl-carbacerulenin, a carbocyclic analog of cerulenin.

    abstract::2,3-Epoxy-4-hydroxy-4-((E,E)-3,6-octadienyl)cyclopentanone (dl-carbacerulenin 5) was synthesized via the epoxyketones 15a and 15b as a mimic of the active form of the antibiotics cerulenin 1, a potent inhibitor of fatty acid synthetase (FAS). The monobenzyl ethers (12 and 13), synthetic intermediates of 15, were prepa...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Shimazawa R,Ogawa Y,Morisaki N,Funabashi H,Kawaguchi A,Iwasaki S

    更新日期:1992-11-01 00:00:00

  • Enzymatic generation of alloxan radicals in rat liver microsomes: possible participation of reduced nicotinamide adenine dinucleotide phosphate (NADPH)-cytochrome P-450 reductase.

    abstract::Electron spin resonance studies showed that addition of rat liver microsomes to the reaction system of alloxan with reduced nicotinamide adenine dinucleotide phosphate (NADPH) resulted in a marked increase in the generation of alloxan radicals (AH.), whereas heat-denatured microsomes were without such effect. Oxidatio...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Sakurai K,Haga K,Ogiso T

    更新日期:1992-02-01 00:00:00

  • Amino acids and peptides. XX. Preparation of beta-cyclododecyl aspartate and its application to synthesis of fibronectin- and laminin-related peptides.

    abstract::beta-Cyclododecyl aspartate was prepared and its application to peptide synthesis was examined. Derivatives of beta-cyclododecyl aspartate are more likely to crystallize and should be useful for peptide synthesis. The cyclododecyl ester was much stable to bases than the benzyl ester and rather more stable than the cyc...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kawasaki K,Murakami T,Koshino K,Namikawa M,Maeda M,Hama T,Mayumi T

    更新日期:1994-04-01 00:00:00

  • Synthesis and structure-activity relationships in a series of ethenesulfonamide derivatives, a novel class of endothelin receptor antagonists.

    abstract::In the previous paper, we described a series of the 2-arylethenesulfonamide derivatives, a novel class of ETA-selective endothelin (ET) receptor antagonists, including the compounds 1a, b. Compound 1a showed excellent oral antagonistic activities and pharmacokinetic profiles, and the monopotassium salt of 1 (YM-598 mo...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Harada H,Kazami J,Watanuki S,Tsuzuki R,Sudoh K,Fujimori A,Tokunaga T,Tanaka A,Tsukamoto S,Yanagisawa I

    更新日期:2001-12-01 00:00:00

  • Cyclodextrin-Based Molecular Accessories for Drug Discovery and Drug Delivery.

    abstract::Recently, active pharmaceutical ingredients (APIs) have been dramatically expanding from low-molecular weight drugs to peptides, proteins, antibodies, genes, oligonucleotides, cells, and machines. Therefore to develop pharmaceutical technologies and drug delivery systems (DDS) for these APIs, advanced molecules and no...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章,评审


    authors: Higashi T

    更新日期:2019-01-01 00:00:00

  • Preparation of 4-pentenoic acid ester of Neu5Ac and 4-pentenyl glycoside of Neu5Ac and their application to glycosylation.

    abstract::Novel sialosyl donors, 4-pentenoic acid ester of N-acetylneuraminic acids (Neu5Ac) and 4-pentenyl glycoside of Neu5Ac were successfully prepared from the corresponding per-O-acetylated 2-hydroxy and 2-chloro derivatives of Neu5Ac, respectively and applied to the synthesis of O-sialosides. ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ikeda K,Fukuyo J,Sato K,Sato M

    更新日期:2005-11-01 00:00:00

  • Increase in aminobutyrate aminotransferase and cholineacetyltransferase in cerebrum of aged rats.

    abstract::We previously reported an increase in aminobutyrate aminotransferase (GABA-T) activity in the cerebrum of Alzheimer patients. In the present study, we investigated whether such findings are common in the usual aging process as well. We examined the activity of various enzymes, which were examined in the previous study...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Aoyagi T,Wada T,Kojima F,Nagai M,Harada S,Takeuchi T,Hirokawa K

    更新日期:1990-06-01 00:00:00

  • Taste masking of propiverine hydrochloride by conversion to its free base.

    abstract::The aim of the present study was to mask the bitterness of propiverine hydrochloride (P-4) by converting it to propiverine free base. Fine granules comprising the free base, which was converted from P-4 by desalination, were prepared. By using Fourier transform infrared spectroscopy, thermogravimetry-differential ther...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ogata T,Koide A,Kinoshita M,Ozeki T

    更新日期:2012-01-01 00:00:00

  • A biologically active insulin analogue with modification in the A2 position. [2-Valine-A] sheep insulin.

    abstract::The synthesis and biological evaluation of [2-Valine-A] insulin ([Val2-A]insulin) is reported. In this insulin, the isoleucine residue in position A2, invariant in the majority of mammalian insulins, is substituted by valine. The same substitution, along with four others, occurs naturally in the insulin produced by th...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ohta N,Burke GT,Katsoyannis PG

    更新日期:1989-03-01 00:00:00

  • Ability of Food/Drink to Reduce the Bitterness Intensity of Topiramate as Determined by Taste Sensor Analysis.

    abstract::The purpose of this study was to determine which foods and/or drinks are capable of reducing the bitterness of topiramate when consumed together with the medicine. The inhibitory effects of foods/drinks (yoghurt and nine other foods/drinks) on the bitterness of topiramate (5 mg/mL) were evaluated with a taste sensor u...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Haraguchi T,Uchida T,Hazekawa M,Yoshida M,Nakashima M,Sanda H,Hase T,Tomoda Y

    更新日期:2016-01-01 00:00:00

  • Tyrosine formation from phenylalanine by ultraviolet irradiation.

    abstract::When phenylalanine was irradiated at ultraviolet (UV) light, p-tyrosine, m-tyrosine and o-tyrosine were identified as hydroxylated products. From p-tyrosine and m-tyrosine, the formation of L-3,4-dihydroxyphenylalanine (DOPA) was observed. The hydroxylation of phenylalanine was prevented by radical scavengers, e.g., c...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Ishimitsu S,Fujimoto S,Ohara A

    更新日期:1990-05-01 00:00:00

  • High entrapment of insulin and bovine serum albumin into neutral and positively-charged liposomes by the remote loading method.

    abstract::Remote loading of insulin and bovine serum albumin (BSA) into neutral or positively-charged liposomes by incubation under a transmembrane pH gradient or non-pH gradient was investigated. Trapping efficiencies in several incubation conditions were compared with those of the conventional reverse-phase evaporation vesicl...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Hwang SH,Maitani Y,Takayama K,Nagai T

    更新日期:2000-03-01 00:00:00

  • Lignan Diesters of Canangafruticoside A from the Leaves of Cananga odorata var. odorata.

    abstract::From the leaves of Cananga odorata var. odorata, three relatively large molecules, namely two aryl naphthalene lignan diesters of canangafruticoside A and one cyclobutane lignan diester of canangafruticoside A, were isolated along with four known compounds. The structures of the new compounds were elucidated based on ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Katsui H,Sugimoto S,Matsunami K,Otsuka H,Lhieochaiphant S

    更新日期:2017-01-01 00:00:00

  • Production of sesquiterpene-type phytoalexins by hairy roots of Hyoscyamus albus co-treated with cupper sulfate and methyl jasmonate.

    abstract::The production of sesquiterpene-type phytoalexins with a vetispyradiene skeleton by Hyoscyamus albus hairy roots induced by methyl jasmonate (MeJA) was reported in a previous paper. The production pattern on co-treatment with cupper sulfate and MeJA (CuSO(4)-MeJA) showed a TLC profile differing from that on treatment ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kawauchi M,Arima T,Shirota O,Sekita S,Nakane T,Takase Y,Kuroyanagi M

    更新日期:2010-07-01 00:00:00

  • Inhibitors of adhesion molecules expression; the synthesis and pharmacological properties of 10H-pyrazino[2,3-b][1,4]benzothiazine derivatives.

    abstract::During a search for novel, orally-active inhibitors of upregulation of adhesion molecules such as intercellular adhesion molecule-1 (ICAM-1), we found a new series of 10H-pyrazino[2,3-b][1,4]benzothiazine derivatives to be potent ICAM-1 inhibitors. Of these compounds, N-[1-(10H-Pyrazino[2,3-b][1,4]benzothiazin-8-ylmet...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kaneko T,Clark RS,Ohi N,Kawahara T,Akamatsu H,Ozaki F,Kamada A,Okano K,Yokohama H,Muramoto K,Ohkuro M,Takenaka O,Kobayashi S

    更新日期:2002-07-01 00:00:00

  • On the interaction of doxorubicin with oleate ions: fluorescence spectroscopy and liquid-liquid extraction study.

    abstract::Increase of lipophilicity of cationic doxorubicin (DOX) by its association with a fatty acid ion is of interest for pharmaceutical formulations and could have an impact on the drug delivery into cancer cells. On the basis of spectroscopic analysis of intrinsic DOX fluorescence, this study provides an experimental evid...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Munnier E,Tewes F,Cohen-Jonathan S,Linassier C,Douziech-Eyrolles L,Marchais H,Soucé M,Hervé K,Dubois P,Chourpa I

    更新日期:2007-07-01 00:00:00

  • Possible mechanism of the stimulatory effect of Artemisia leaf extract on the proliferation of cultured endothelial cells: involvement of basic fibroblast growth factor.

    abstract::To investigate the possible mechanism of the stimulatory effect of a hot water extract from Artemisia leaf (Artemisia princeps PANPANINI) (AFE) on the proliferation of endothelial cells, cells from bovine aorta were cultured for 72 h in RPMI1640 medium supplemented with 10% fetal calf serum in the presence of 5 microg...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kaji T,Kaga K,Miezi N,Hayashi T,Ejiri N,Sakuragawa N

    更新日期:1990-09-01 00:00:00

  • Design, Synthesis and Biological Evaluation of Oxindole-Based Chalcones as Small-Molecule Inhibitors of Melanogenic Tyrosinase.

    abstract::The enzyme tyrosinase regulates melanogenesis and skin hyperpigmentation by converting L-3,4-dihydroxyphenylalanine (L-DOPA) into dopaquinone, a key step in the melanin biosynthesis. The present work deals with design and synthesis of various oxindole-based chalcones as monophenolase and diphenolase activity inhibitor...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Suthar SK,Bansal S,Narkhede N,Guleria M,Alex AT,Joseph A

    更新日期:2017-01-01 00:00:00

  • A novel method for estimation of transition temperature for polymorphic pairs in pharmaceuticals using heat of solution and solubility data.

    abstract::A novel method for thermodynamic stability studies of polymorphic drug substances has been developed. In order to estimate the transition temperature for an enantiotropic polymorphic pair, a formula for calculating the temperature at which the solubilities of each polymorph become equal has been derived with heat of s...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Urakami K,Shono Y,Higashi A,Umemoto K,Godo M

    更新日期:2002-02-01 00:00:00

  • New iridoids from Gelsemium species.

    abstract::Four new iridoids structurally related to gelsemide (5) were isolated from two Loganiaceous plants, Gelsemium elegans and G. rankinii. Among them, GEIR-1 (1) has a novel tetracyclic caged structure. ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kogure N,Ishii N,Kobayashi H,Kitajima M,Wongseripipatana S,Takayama H

    更新日期:2008-06-01 00:00:00

  • Cytotoxic cardenolides from woods of Euonymus alata.

    abstract::Three cytotoxic cardenolides, acovenosigenin A 3-O-alpha-L-ramnopyranoside (1), euonymoside A (2) and euonymusoside A (3), were isolated from the woods of Euonymus alata (Celastraceae). The chemical structure of a new cardenolide, euonymusoside A (3) has been elucidated on the basis of extensive spectral analysis and ...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Kitanaka S,Takido M,Mizoue K,Nakaike S

    更新日期:1996-03-01 00:00:00

  • Calculation of membrane potential in synaptosomes with use of a lipophilic cation (tetraphenylphosphonium).

    abstract::To estimate membrane potential in synaptosomes with the use of tetraphenylphosphonium (TPP+), an equation relating the amount of TPP+ accumulated in synaptosomes with membrane potential was derived from the following two assumptions. (1) TPP+ molecules were distributed into plasma membranes, mitochondria and cytosol o...

    journal_title:Chemical & pharmaceutical bulletin

    pub_type: 杂志文章


    authors: Aiuchi T,Matsunaga M,Nakaya K,Nakamura Y

    更新日期:1989-12-01 00:00:00