The L type calcium current in single hypertrophied cardiomyocytes isolated from the right ventricle of ferret heart.


OBJECTIVE:The aim was to study L type calcium current alterations in relation to the action potential lengthening induced by hypertrophy in isolated cardiomyocytes from the right ventricle of ferret. METHODS:Chronic pulmonary artery constriction was established in adult male ferrets under anaesthesia. Sham operated animals were used as controls. Four to six weeks later the heart was excised and treated with a mixed collagenase-elastase solution to isolate the right ventricular myocytes. The calcium current was investigated in control and hypertrophied cells with the whole cell configuration of the patch clamp technique. The validity of the model was tested by analysis of the structural and passive electrical characteristics of the cells, which were enzymatically isolated from right ventricles previously overloaded (4 to 6 weeks) by clipping the pulmonary artery. RESULTS:Isolated cells from right ventricles submitted to a chronic pressure overload had well preserved cellular integrity suggesting the absence of myocardial failure. This compensated form of hypertrophy was characterised by a dilated transverse tubular system, which could explain the increased membrane capacity. Such cells developed a prolonged action potential with a less pronounced fast repolarisation phase inducing a higher plateau phase. When studied in physiological Tyrode solution the density and kinetics of the L type calcium current were not apparently modified, but a significant decrease in density was unmasked when sodium and potassium currents were suppressed by external and internal substitution of sodium and potassium by tetraethyl ammonium. CONCLUSIONS:The decrease in L type calcium current cannot be involved in the lengthening of action potential observed on hypertrophied myocytes, but it could account for the depressed contractile activity. A noticeable decrease of the transient outward current is suggested to explain the action potential alterations.


Cardiovasc Res


Cardiovascular research


Bouron A,Potreau D,Raymond G




Has Abstract


1992-07-01 00:00:00












  • Adenovirus-mediated expression of p35 prevents hypoxia/reoxygenation injury by reducing reactive oxygen species and caspase activity.

    abstract:OBJECTIVE:This study aimed to examine the effects of adenovirus-mediated expression of p35, a baculovirus gene, on apoptosis induced by hypoxia/reoxygenation (H/R) in cardiomyocytes. METHODS:Neonatal rat cardiomyocytes were infected with recombinant adenoviral vectors expressing p35 (Ad2/CMVp35) or no transgene (Ad2/C...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Date T,Belanger AJ,Mochizuki S,Sullivan JA,Liu LX,Scaria A,Cheng SH,Gregory RJ,Jiang C

    更新日期:2002-08-01 00:00:00

  • Role of human smooth muscle cell progenitors in atherosclerotic plaque development and composition.

    abstract:AIMS:We analysed the possible protective role of human endothelial (EPCs) and smooth muscle (SPCs) progenitor cells on atherosclerosis development in apoE(-/-)RAG2(-/-) mice. We determined plasma levels of SPCs in coronary patients. METHODS AND RESULTS:ApoE(-/-)RAG2(-/-) mice received four intravenous injections of sa...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Zoll J,Fontaine V,Gourdy P,Barateau V,Vilar J,Leroyer A,Lopes-Kam I,Mallat Z,Arnal JF,Henry P,Tobelem G,Tedgui A

    更新日期:2008-02-01 00:00:00

  • Lack of effect of anti-hypertensive treatment with felodipine on cardiovascular structure of young spontaneously hypertensive rats.

    abstract::Felodipine (4(2,3-dichlorophenyl)-1,4-dihydro-2, 6-dimethyl-3-ethoxycarbonyl-5-methoxycarbonyl pyridine)), a selective vasodilating anti-hypertensive drug, was used in the treatment of spontaneously hypertensive rats (SHRs) and age-matched Wistar-Kyoto rats (WKYs) from age 6 to 14 weeks, ie during the time of high blo...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Nyborg NC,Mulvany MJ

    更新日期:1985-09-01 00:00:00

  • Fibrinolytic therapy in subacute bacterial endocarditis: an experimental study.

    abstract::The effect of adding fibrinolytic to penicillin therapy in experimentally induced streptococcal bacterial endocarditis has been studied in the rabbit. In 9 day old infected lesions, the vegetations could be substantially reduced in size after a 3 day course of intravenous streptokinase. Quantitative microbiological te...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Johnson CE,Dewar HA,Aherne WA

    更新日期:1980-08-01 00:00:00

  • Hydrogen sulphide is an inhibitor of L-type calcium channels and mechanical contraction in rat cardiomyocytes.

    abstract:AIMS:Hydrogen sulphide (H(2)S) is an endogenously generated gaseous transmitter that has recently been suggested to regulate cardiovascular functions. To date, there is no direct evidence for a potential role of H(2)S in regulating calcium channels in the heart. The present study aims to examine the hypothesis that H(2...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Sun YG,Cao YX,Wang WW,Ma SF,Yao T,Zhu YC

    更新日期:2008-09-01 00:00:00

  • Remodelling and functional alterations of the rabbit coronary artery in volume overloaded heart.

    abstract:OBJECTIVE:The aim was to study the contractility of the conduit coronary artery to vasoactive agents in developing and established volume overload cardiac hypertrophy and to compare it with structural alterations in the artery. METHODS:Aortic valve insufficiency in rabbits was used to produce a volume overloaded heart...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Gerová M,Holécyová A,Kristek F,Fízel' A,Fízel'ová A

    更新日期:1993-11-01 00:00:00

  • Controversies on the sensitivity of the diabetic heart to ischemic injury: the sensitivity of the diabetic heart to ischemic injury is decreased.

    abstract::Controversy exists as to whether the diabetic heart is more or less sensitive to ischemic injury. Although a considerable number of experimental studies have directly determined the effects of ischemia on the diabetic heart, there is still no general agreement as to whether metabolic changes within the myocardium cont...

    journal_title:Cardiovascular research

    pub_type: 杂志文章,评审


    authors: Feuvray D,Lopaschuk GD

    更新日期:1997-04-01 00:00:00

  • Benefits of reperfusion beyond infarct size limitation.

    abstract::The most critical determinant of prognosis in patients with acute myocardial infarction (MI) is infarct magnitude, which can be established within several hours of an attack. The importance of the subsequent healing process is not negligible, however. In fact, much experimental and clinical evidence suggests that late...

    journal_title:Cardiovascular research

    pub_type: 杂志文章,评审


    authors: Takemura G,Nakagawa M,Kanamori H,Minatoguchi S,Fujiwara H

    更新日期:2009-07-15 00:00:00

  • Effect of increases in afterload before and after coronary occlusion in awake dogs.

    abstract::Ventricular function curves relating stroke work and left ventricular end-diastolic pressure were generated in awake dogs during increases in preload produced by infusion of fluid and during increases in afterload produced by administration of phenylephrine. The ventricular function curves produced by preloading were ...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Rosenfeldt FL,Cobb FR,Bache RJ,Sabiston DC Jr

    更新日期:1979-07-01 00:00:00

  • Adventitial fibroblast reactive oxygen species as autacrine and paracrine mediators of remodeling: bellwether for vascular disease?

    abstract::The importance of the vascular adventitia is increasingly being recognized not only in vascular disease but also in normal maintenance and homeostasis of vessels. Activation of the adventitia and its resident fibrocytic cells in response to injury, stretch, cytokines, and hormones has been shown to stimulate different...

    journal_title:Cardiovascular research

    pub_type: 杂志文章,评审


    authors: Haurani MJ,Pagano PJ

    更新日期:2007-09-01 00:00:00

  • Evaluation of transmitral pressure gradients at different heart rates: divergent action of isoprenaline and atropine.

    abstract:STUDY OBJECTIVE:The aim was to record diastolic transmitral pressure gradients at high sensitivity to quantitate the effect on transmitral pressure gradients of changing the heart rate. DESIGN:Diastolic left atrial and left ventricular pressures were recorded at high sensitivity (40 mm Hg = 10 mm recorded deflection) ...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Carmeliet P,Aubert A,Van de Werf F,De Geest H

    更新日期:1990-07-01 00:00:00

  • HIV infection, highly active antiretroviral therapy and the cardiovascular system.

    abstract::Cardiovascular complications in the course of human immunodeficiency virus (HIV) infection are multifactorial and may be caused by the virus itself or by the related opportunistic infections and neoplasms. Highly active antiretroviral therapy (HAART) has prolonged many patients' lives, but many cardiac sequelae of HIV...

    journal_title:Cardiovascular research

    pub_type: 杂志文章,评审


    authors: Barbaro G

    更新日期:2003-10-15 00:00:00

  • Ionic basis of action potential prolongation of hypertrophied cardiac myocytes isolated from hypertensive rats of different ages.

    abstract:OBJECTIVE:The aim was to determine the ionic basis of action potential lengthening associated with cardiac hypertrophy. METHODS:Action potentials and ionic currents of left ventricular myocytes isolated from normal and hypertrophied hearts were recorded with patch pipettes. Since cardiac hypertrophy is a time dependen...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Cerbai E,Barbieri M,Li Q,Mugelli A

    更新日期:1994-08-01 00:00:00

  • Catecholamine stimulation is associated with impaired myocardial O(2) utilization in heart failure.

    abstract:OBJECTIVES:To investigate the effect of alpha,beta(1) and beta(2) adrenergic receptor (AR) stimulation on coronary hemodynamics, myocardial oxygen consumption (M(v)O(2)) and metabolic substrate preference in advanced dilated cardiomyopathy (DCM). METHODS:We studied 19 conscious, instrumented dogs with pacing-induced D...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Nikolaidis LA,Hentosz T,Doverspike A,Huerbin R,Stolarski C,Shen YT,Shannon RP

    更新日期:2002-02-01 00:00:00

  • Flt-1 (VEGFR-1) coordinates discrete stages of blood vessel formation.

    abstract:AIMS:In developing blood vessel networks, the overall level of vessel branching often correlates with angiogenic sprout initiations, but in some pathological situations, increased sprout initiations paradoxically lead to reduced vessel branching and impaired vascular function. We examine the hypothesis that defects in ...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Chappell JC,Cluceru JG,Nesmith JE,Mouillesseaux KP,Bradley VB,Hartland CM,Hashambhoy-Ramsay YL,Walpole J,Peirce SM,Mac Gabhann F,Bautch VL

    更新日期:2016-07-01 00:00:00

  • In vivo alterations in cardiac metabolism and function in the spontaneously hypertensive rat heart.

    abstract:AIMS:The aim of this work was to use hyperpolarized carbon-13 ((13)C) magnetic resonance (MR) spectroscopy and cine MR imaging (MRI) to assess in vivo cardiac metabolism and function in the 15-week-old spontaneously hypertensive rat (SHR) heart. At this time point, the SHR displays hypertension and concentric hypertrop...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Dodd MS,Ball DR,Schroeder MA,Le Page LM,Atherton HJ,Heather LC,Seymour AM,Ashrafian H,Watkins H,Clarke K,Tyler DJ

    更新日期:2012-07-01 00:00:00

  • Is there a transient rise in sub-sarcolemmal Na and activation of Na/K pump current following activation of I(Na) in ventricular myocardium?

    abstract:OBJECTIVE:The primary aim of this study was to investigate whether activation of Na influx via voltage-gated Na channels can elevate sub-sarcolemmal ('fuzzy-space') [Na] and transiently activate Na/K pump current (I(p)). METHODS AND RESULTS:Initially, Na/K pump activity was characterised in whole-cell voltage-clamped ...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Silverman Bd,Warley A,Miller JI,James AF,Shattock MJ

    更新日期:2003-03-15 00:00:00

  • Cardiac angiotensin converting enzyme overproduction indicates interstitial activation in renovascular hypertension.

    abstract:OBJECTIVES:Angiotensin converting enzyme (ACE) activity in the plasma does not change significantly with hypertension in two-kidney, one-clip hypertensive (2K-1C) rats. However, heart ACE activity and mRNA increase with hypertension. We measured the ACE activity and mRNA in hypertrophied hearts at different times after...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Challah M,Nicoletti A,Arnal JF,Philippe M,Laboulandine I,Allegrini J,Alhenc-Gelas F,Danilov S,Michel JB

    更新日期:1995-08-01 00:00:00

  • Non-invasive assessment of the age related changes in stiffness of major branches of the human arteries.

    abstract::The static mechanical properties of major branches of the human arteries (common carotid artery, abdominal aorta, femoral artery, and brachial artery) were studied in 39 subjects, aged 6-81 years, using an ultrasonic phase locked echo tracking system that allows continuous transcutaneous measurement of the diameter of...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Kawasaki T,Sasayama S,Yagi S,Asakawa T,Hirai T

    更新日期:1987-09-01 00:00:00

  • Selection of saphenous vein bypass gradt diameter to support patency of the stenosed coronary artery.

    abstract::Injudicious selection of a saphenous vein graft can adversely affect the the late postoperative patency of the proximal coronary artery which it bypasses. The purpose of this investigation was to deduce an upper bound for graft diameter, at small angle of distal anastomosis, below which the stenosed artery will remain...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Moskowitz SE,Lewis BS,Milo S,Amar R

    更新日期:1979-07-01 00:00:00

  • A decade of genome-wide association studies for coronary artery disease: the challenges ahead.

    abstract::In this review, we summarize current knowledge on the genetics of coronary artery disease, based on 10 years of genome-wide association studies. The discoveries began with individual studies using 200K single nucleotide polymorphism arrays and progressed to large-scale collaborative efforts, involving more than a 100 ...

    journal_title:Cardiovascular research

    pub_type: 历史文章,杂志文章,评审


    authors: Erdmann J,Kessler T,Munoz Venegas L,Schunkert H

    更新日期:2018-07-15 00:00:00

  • Identification and immunolocalisation of annexins V and VI, the major cardiac annexins, in rat heart.

    abstract:OBJECTIVE:The heart contains proteins of the annexin family, a unique group of calcium binding proteins. This study was aimed at identifying the major cardiac annexins and determining their distribution in the rat heart. METHODS:Annexins were isolated by affinity chromatography and purified by ion exchange high pressu...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Doubell AF,Lazure C,Charbonneau C,Thibault G

    更新日期:1993-07-01 00:00:00

  • Taurine depresses INa and depolarises the membrane but does not affect membrane surface charges in perfused rabbit hearts.

    abstract:STUDY OBJECTIVE:The aim was to determine whether taurine influences the membrane surface charges in cardiac muscle. DESIGN:Screening of the negative charges at the outside surface of the membrane results in a shift of the steady state inactivation of the sodium system towards less negative potentials. This feature was...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Dumaine R,Schanne OF,Ruiz-Petrich E

    更新日期:1990-11-01 00:00:00

  • Changes in regional coronary blood flow with hypertonic mannitol in conscious dogs.

    abstract::Systemic haemodynamics and regional myocardial blood flow responses to hypertonic mannitol were studied in 10 conscious and 23 anaesthetized dogs. Mannitol infusion significantly increased regional myocardial blood flow in the conscious, intact dogs. Mannitol increased total coronary flow 20% in anasethetized animals ...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Hutton I,Marynick SP,Fixler DE,Templeton GH,Willerson JT

    更新日期:1975-01-01 00:00:00

  • Angiotensin II, adhesion, and cardiac fibrosis.

    abstract::Angiotensin II (AII) plays a critical role in cardiac remodeling. This peptide promotes cardiac myocyte hypertrophy and cardiac fibroblast interstitial fibrotic changes associated with left ventricular hypertrophy, post myocardial infarction remodeling and congestive heart failure. AII mediates cardiac myocyte hypertr...

    journal_title:Cardiovascular research

    pub_type: 杂志文章,评审


    authors: Schnee JM,Hsueh WA

    更新日期:2000-05-01 00:00:00

  • Mediators of perivascular inflammation in the left ventricle of renovascular hypertensive rats.

    abstract:OBJECTIVE:Inflammatory cells invade the fibrotic myocardium of spontaneously hypertensive rats at the same sites as where fibroblasts are produced. The role of these inflammatory cells in myocardial fibrogenesis was studied in the present work. METHODS:The production and distribution of proteins that may be implicated...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Nicoletti A,Mandet C,Challah M,Bariéty J,Michel JB

    更新日期:1996-04-01 00:00:00

  • Cardiac transplantation affects ventricular expression of brain natriuretic peptide.

    abstract:OBJECTIVE:The aim was to examine the ventricular expression of brain natriuretic peptide (BNP) in the transplanted human heart. METHODS:Serial right ventricular biopsies (n = 68) and plasma samples (n = 68) were obtained for measurement of BNP and atrial natriuretic peptide (ANP) from 14 orthotopic cardiac transplant ...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Ationu A,Burch M,Singer D,Littleton P,Carter N

    更新日期:1993-02-01 00:00:00

  • Role of microRNAs in the reperfused myocardium towards post-infarct remodelling.

    abstract::Myocardial ischaemia/reperfusion (I/R)-induced remodelling generally includes cell death (necrosis and apoptosis), myocyte hypertrophy, angiogenesis, cardiac fibrosis, and myocardial dysfunction. It is becoming increasingly clear that microRNAs (miRNAs or miRs), a group of highly conserved small (∼18-24 nucleotide) no...

    journal_title:Cardiovascular research

    pub_type: 杂志文章,评审


    authors: Zhu H,Fan GC

    更新日期:2012-05-01 00:00:00

  • A current understanding of drug-induced QT prolongation and its implications for anticancer therapy.

    abstract::The QT interval, a global index of ventricular repolarization, varies among individuals and is influenced by diverse physiologic and pathophysiologic stimuli such as gender, age, heart rate, electrolyte concentrations, concomitant cardiac disease, and other diseases such as diabetes. Many drugs produce a small but rep...

    journal_title:Cardiovascular research

    pub_type: 杂志文章,评审


    authors: Roden DM

    更新日期:2019-04-15 00:00:00

  • The effects of sepsis on endothelium and clinical implications.

    abstract:ABSTRACT:Sepsis accounts for nearly 700 000 deaths in Europe annually and is caused by an overwhelming host response to infection resulting in organ failure. The endothelium is an active contributor to sepsis and as such represents a major target for therapy. During sepsis, endothelial cells amplify the immune response...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Dolmatova EV,Wang K,Mandavilli R,Griendling KK

    更新日期:2021-01-01 00:00:00