Effects of regional ischaemia, with or without reperfusion, on endothelium dependent coronary relaxation in the dog.


OBJECTIVE:The aim was to establish whether the duration of coronary ischaemia and coronary ischaemia with reperfusion selectively reduced the magnitude of relaxation mediated by endothelium dependent relaxing factor (EDRF) in response to thrombin, compared with relaxation produced by acetylcholine and calcimycin. METHODS:Adult male dogs, anaesthetised with sodium pentobarbitone (30 mg.kg-1 intravenously) were used. Coronary artery occlusions were maintained for either 15 or 45 min; in half the dogs from each timepoint, occlusion was followed by 60 min reperfusion. At the end of each in situ period, coronary arteries were removed from both ischaemic and non-ischaemic regions, cut into rings, and hung in isolated organ baths. Dose-response relationships to the EDRF dependent vasodilators thrombin, acetylcholine, and calcimycin, and to the EDRF independent vasodilator isoprenaline, were then established. RESULTS:Thrombin (0.003-0.3 units.ml-1) caused dose dependent relaxation in all tissues. Relaxant responses (E(max)) in the non-ischaemic vessels from both 15 and 45 min treatment groups were used as control data for the responses in ischaemic vessels. Maximum responses were not different in the non-ischaemic groups from either 15 or 45 min studies, at 82.7 (SEM 3.7)% after 15 min, and 82.1(2.4)% after 45 min. There was a small but significant reduction in E(max) after 15 min and 45 min ischaemia, to 74.4(3.2)% and 74.4(3.0)% respectively. Sixty minutes reperfusion provoked a further reduction in E(max) to 64.9(3.8)% after 45 min ischaemia, but not after 15 min ischaemia [70.3(4.2)%]. Neither 15 nor 45 min interventions altered E(max) of relaxation to acetylcholine or calcimycin (greater than 88.0% in each group). Similarly there were no significant differences between groups to the relaxation stimulated by isoprenaline (E(max) greater than 90.0%). CONCLUSIONS:The data suggest that loss of EDRF dependent relaxation to thrombin is more sensitive to ischaemia than the relaxation produced by either acetylcholine or calcimycin, and appears to be manifested early in the onset of ischaemic injury.


Cardiovasc Res


Cardiovascular research


Winn MJ,Ku DD




Has Abstract


1992-03-01 00:00:00












  • Enigma homolog 1 scaffolds protein kinase D1 to regulate the activity of the cardiac L-type voltage-gated calcium channel.

    abstract:AIMS:In cardiomyocytes, protein kinase D1 (PKD1) plays a central role in the response to stress signals. From a yeast two-hybrid assay, we have identified Enigma Homolog 1 (ENH1) as a new binding partner of PKD1. Since in neurons, ENH1, associated with protein kinase Cepsilon, was shown to modulate the activity of N-ty...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Maturana AD,Wälchli S,Iwata M,Ryser S,Van Lint J,Hoshijima M,Schlegel W,Ikeda Y,Tanizawa K,Kuroda S

    更新日期:2008-06-01 00:00:00

  • Carvedilol reverses cardiac insufficiency in AKAP5 knockout mice by normalizing the activities of calcineurin and CaMKII.

    abstract:AIMS:Cardiac β-adrenergic receptors (β-AR) are key regulators of cardiac haemodynamics and size. The scaffolding protein A-kinase anchoring protein 79/150 (AKAP5) is a key regulator of myocardial signalling by β-ARs. We examined the function of AKAP5 in regulating cardiac haemodynamics and size, and the role of β-ARs a...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Li X,Matta SM,Sullivan RD,Bahouth SW

    更新日期:2014-11-01 00:00:00

  • Reverse rate dependency is an intrinsic property of canine cardiac preparations.

    abstract:AIMS:Class III antiarrhythmic agents exhibit reverse rate-dependent lengthening of the action potential duration (APD). In spite of the several theories developed so far to explain this reverse rate dependency (RRD), its mechanism has not yet been clarified. The aim of the present work was to further elucidate the mech...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Bányász T,Horváth B,Virág L,Bárándi L,Szentandrássy N,Harmati G,Magyar J,Marangoni S,Zaza A,Varró A,Nánási PP

    更新日期:2009-11-01 00:00:00

  • Nuclear accumulation of androgen receptor in gender difference of dilated cardiomyopathy due to lamin A/C mutations.

    abstract:AIMS:Dilated cardiomyopathy (DCM) is characterized by ventricular dilation associated with systolic dysfunction, which could be caused by mutations in lamina/C gene (LMNA). LMNA-linked DCM is severe in males in both human patients and a knock-in mouse model carrying a homozygous p.H222P mutation (LmnaH222P/H222P). The ...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Arimura T,Onoue K,Takahashi-Tanaka Y,Ishikawa T,Kuwahara M,Setou M,Shigenobu S,Yamaguchi K,Bertrand AT,Machida N,Takayama K,Fukusato M,Tanaka R,Somekawa S,Nakano T,Yamane Y,Kuba K,Imai Y,Saito Y,Bonne G,Kimura A

    更新日期:2013-08-01 00:00:00

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    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Li HY,Liu XL,Liu YT,Jia ZK,Filep JG,Potempa LA,Ji SR,Wu Y

    更新日期:2019-02-01 00:00:00

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    pub_type: 杂志文章


    authors: Bito V,de Waard MC,Biesmans L,Lenaerts I,Ozdemir S,van Deel E,Abdel-Mottaleb Y,Driesen R,Holemans P,Duncker DJ,Sipido KR

    更新日期:2010-04-01 00:00:00

  • Cardioprotection with adenosine metabolism inhibitors in ischemic-reperfused mouse heart.

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    pub_type: 杂志文章


    authors: Peart J,Matherne GP,Cerniway RJ,Headrick JP

    更新日期:2001-10-01 00:00:00

  • Nitrated fatty acids suppress angiotensin II-mediated fibrotic remodelling and atrial fibrillation.

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    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Rudolph TK,Ravekes T,Klinke A,Friedrichs K,Mollenhauer M,Pekarova M,Ambrozova G,Martiskova H,Kaur JJ,Matthes B,Schwoerer A,Woodcock SR,Kubala L,Freeman BA,Baldus S,Rudolph V

    更新日期:2016-01-01 00:00:00

  • Additive cardioprotection by pharmacological postconditioning with hydrogen sulfide and nitric oxide donors in mouse heart: S-sulfhydration vs. S-nitrosylation.

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    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Sun J,Aponte AM,Menazza S,Gucek M,Steenbergen C,Murphy E

    更新日期:2016-05-01 00:00:00

  • Separation of the oscillatory current from other currents in cardiac Purkinje fibres.

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    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Mugelli A

    更新日期:1982-11-01 00:00:00

  • Physiological And Pathological Roles Of Protein Kinase A In The Heart.

    abstract::Protein kinase A (PKA) is a central regulator of cardiac performance and morphology. Myocardial PKA activation is induced by a variety of hormones, neurotransmitters and stress signals, most notably catecholamines secreted by the sympathetic nervous system. Catecholamines bind β-adrenergic receptors to stimulate cAMP-...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Liu Y,Chen J,Fontes SK,Bautista EN,Cheng Z

    更新日期:2021-01-23 00:00:00

  • Adenosine receptor specificity in preconditioning of isolated rabbit cardiomyocytes: evidence of A3 receptor involvement.

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    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Armstrong S,Ganote CE

    更新日期:1994-07-01 00:00:00

  • Late-phase detection of recent myocardial ischaemia using ultrasound molecular imaging targeted to intercellular adhesion molecule-1.

    abstract:AIMS:in this study, we attempted to detect a recent myocardial ischaemic event using ultrasound molecular imaging (UMI) with microbubbles (MB) targeted to intercellular adhesion molecule-1 (ICAM-1) in the late phase of reperfusion. METHODS AND RESULTS:we created a myocardial ischaemia-reperfusion model in 60 C57/BL ma...

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    authors: Yan Y,Liao Y,Yang L,Wu J,Du J,Xuan W,Ji L,Huang Q,Liu Y,Bin J

    更新日期:2011-01-01 00:00:00

  • Noninvasive determination of the mitral valve area in stenosis: a computational model and correlation with autopsy and open heart measurements.

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    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Seitz WS,Operschall EJ

    更新日期:1980-04-01 00:00:00

  • Dose response in vivo to digoxin in normo-and hyperkalaemia: associated biochemical changes.

    abstract::The effect of digoxin, at two different inotropic levels, was examined in normo- and hyperkalaemic dogs. For similar inotropic responses, normo- and hyperkalaemic dogs had similar levels of (Na+, K+)-ATPase inhibition and microsomal-bound digoxin. ...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Goldman RH,Coltart DJ,Schweizer E,Snidow G,Harrison DC

    更新日期:1975-07-01 00:00:00

  • Overexpression of long or short FGFR-1 results in FGF-2-mediated proliferation in neonatal cardiac myocyte cultures.

    abstract:OBJECTIVE:The type 1 fibroblast growth factor receptor (FGFR-1) is the only high affinity receptor for fibroblast growth factor-2 (FGF-2) in the rat myocardium, and is essential for normal growth and development of the heart. Levels of FGFR-1 are developmentally regulated, being high in embryonic cardiac myocytes. Also...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Sheikh F,Fandrich RR,Kardami E,Cattini PA

    更新日期:1999-06-01 00:00:00

  • GSK-3β at the crossroads in the signalling of heart preconditioning: implication of mTOR and Wnt pathways.

    abstract:AIMS:Ischaemic preconditioning (IPC) protects the heart against prolonged lethal ischaemia through a signalling cascade involving Akt, glycogen synthase kinase-3β (GSK-3β), and mitochondrial ATP-sensitive potassium channels (mitoK(ATP)). We previously demonstrated the involvement of the Wnt pathway in IPC in vivo via G...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Vigneron F,Dos Santos P,Lemoine S,Bonnet M,Tariosse L,Couffinhal T,Duplaà C,Jaspard-Vinassa B

    更新日期:2011-04-01 00:00:00

  • Metabolic alterations in the chronically denervated dog heart.

    abstract:OBJECTIVE:Previous studies have shown that chronic cardiac denervation impairs myocardial glucose oxidation. To investigate this further we tested whether the tissue content of glucose transporters, activity of glycolytic enzymes or metabolic capacity of pyruvate dehydrogenase were altered. Moreover, we investigated wh...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: van der Vusse GJ,Dubelaar ML,Coumans WA,Seymour AM,Clarke SB,Bonen A,Drake-Holland AJ,Noble MI

    更新日期:1998-01-01 00:00:00

  • Effects of oxidised low density lipoprotein on dendritic cells: a possible immunoregulatory component of the atherogenic micro-environment?

    abstract:OBJECTIVE:The objective of this study was to explore the relationship between low density lipoprotein (LDL) and dendritic cell (DC) activation, based upon the hypothesis that reactive oxygen species (ROS)-mediated modification of proteins that may be present in local DC microenvironments could be important as mediators...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Alderman CJ,Bunyard PR,Chain BM,Foreman JC,Leake DS,Katz DR

    更新日期:2002-09-01 00:00:00

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    abstract:AIMS:Ivabradine (Iva) has shown beneficial structural and functional effects in clinical and experimental heart failure (HF), but its action in combination with mechanical unloading (MU), such as during treatment with left ventricular assist devices (LVAD), is unknown. The aim of this study was to investigate the effec...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Navaratnarajah M,Ibrahim M,Siedlecka U,van Doorn C,Shah A,Gandhi A,Dias P,Sarathchandra P,Yacoub MH,Terracciano CM

    更新日期:2013-02-01 00:00:00

  • Human Influenza A virus causes myocardial and cardiac-specific conduction system infection associated with early inflammation and premature death.

    abstract:AIMS:Human influenza A virus (hIAV) infection is associated with important cardiovascular complications, although cardiac infection pathophysiology is poorly understood. We aimed to study the ability of hIAV of different pathogenicity to infect the mouse heart, and establish the relationship between the infective capac...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Filgueiras-Rama D,Vasilijevic J,Jalife J,Noujaim SF,Alfonso JM,Nicolas-Avila JA,Gutierrez C,Zamarreño N,Hidalgo A,Bernabé A,Cop CP,Ponce-Balbuena D,Guerrero-Serna G,Calle D,Desco M,Ruiz-Cabello J,Nieto A,Falcon A

    更新日期:2020-04-28 00:00:00

  • Identification of cardiac long non-coding RNA profile in human dilated cardiomyopathy.

    abstract:Aims:Dilated cardiomyopathy (DCM) induced chronic heart failure is a life-threatening disease worldwide. Long non-coding RNAs (lncRNAs) are potential new therapeutic targets and may provide new pathophysiological mechanisms for development of DCM. Methods and results:Microarray assays in 14 DCM and 10 control human he...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Li H,Chen C,Fan J,Yin Z,Ni L,Cianflone K,Wang Y,Wang DW

    更新日期:2018-04-01 00:00:00

  • Exchange protein activated by cyclic-adenosine monophosphate (Epac) regulates atrial fibroblast function and controls cardiac remodelling.

    abstract:Aims:Heart failure (HF) produces left atrial (LA)-selective fibrosis and promotes atrial fibrillation. HF also causes adrenergic activation, which contributes to remodelling via a variety of signalling molecules, including the exchange protein activated by cAMP (Epac). Here, we evaluate the effects of Epac1-signalling ...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Surinkaew S,Aflaki M,Takawale A,Chen Y,Qi XY,Gillis MA,Shi YF,Tardif JC,Chattipakorn N,Nattel S

    更新日期:2019-01-01 00:00:00

  • Moderate vs. high exercise intensity: differential effects on aerobic fitness, cardiomyocyte contractility, and endothelial function.

    abstract:OBJECTIVE:Current guidelines are controversial regarding exercise intensity in cardiovascular prevention and rehabilitation. Although high-intensity training induces larger increases in fitness and maximal oxygen uptake (VO(2max)), moderate intensity is often recommended as equally effective. Controlled preclinical stu...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Kemi OJ,Haram PM,Loennechen JP,Osnes JB,Skomedal T,Wisløff U,Ellingsen Ø

    更新日期:2005-07-01 00:00:00

  • Reduced SERCA2 abundance decreases the propensity for Ca2+ wave development in ventricular myocytes.

    abstract:AIMS:To describe the overall role of reduced sarcoplasmic reticulum Ca(2+) ATPase (SERCA2) for Ca(2+) wave development. METHODS AND RESULTS:SERCA2 knockout [Serca2(flox/flox) Tg(alphaMHC-MerCreMer); KO] mice allowing inducible cardiomyocyte-specific disruption of the Serca2 gene in adult mice were compared with Serca(...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Stokke MK,Hougen K,Sjaastad I,Louch WE,Briston SJ,Enger UH,Andersson KB,Christensen G,Eisner DA,Sejersted OM,Trafford AW

    更新日期:2010-04-01 00:00:00

  • Etv2 as an essential regulator of mesodermal lineage development.

    abstract::The 'master regulatory factors' that position at the top of the genetic hierarchy of lineage determination have been a focus of intense interest, and have been investigated in various systems. Etv2/Etsrp71/ER71 is such a factor that is both necessary and sufficient for the development of haematopoietic and endothelial...

    journal_title:Cardiovascular research

    pub_type: 杂志文章,评审


    authors: Koyano-Nakagawa N,Garry DJ

    更新日期:2017-09-01 00:00:00

  • Hydrogen sulfide accounts for the peripheral vascular effects of zofenopril independently of ACE inhibition.

    abstract:AIMS:Therapeutic use of sulfhydrylated inhibitor S-zofenopril has raised different hypotheses regarding the role played by its thiol group in the beneficial clinical effects exerted compared with other angiotensin-converting enzyme (ACE) inhibitors. Here, we investigated hydrogen sulfide (H2S) pathway as accountable fo...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Bucci M,Vellecco V,Cantalupo A,Brancaleone V,Zhou Z,Evangelista S,Calderone V,Papapetropoulos A,Cirino G

    更新日期:2014-04-01 00:00:00

  • TIMP3 is the primary TIMP to regulate agonist-induced vascular remodelling and hypertension.

    abstract:AIMS:Hypertension is accompanied by structural remodelling of vascular extracellular matrix (ECM). Tissue inhibitor of metalloproteinases (TIMPs) inhibits matrix metalloproteinases (MMPs) that degrade the matrix structural proteins. In response to a hypertensive stimulus, the balance between MMPs and TIMPs is altered. ...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Basu R,Lee J,Morton JS,Takawale A,Fan D,Kandalam V,Wang X,Davidge ST,Kassiri Z

    更新日期:2013-06-01 00:00:00

  • Comparison of the effects of supplementation with whey mineral and potassium on arterial tone in experimental hypertension.

    abstract:OBJECTIVE:The aim of this work was to compare the effects of supplementation of rat chow diet with potassium (K+) and whey mineral concentrate (Whey), a diet rich in milk minerals, on blood pressure and arterial responses in vitro in spontaneously hypertensive rats (SHR). METHODS:Thirty young SHR and twenty Wistar-Kyo...

    journal_title:Cardiovascular research

    pub_type: 杂志文章


    authors: Wu X,Tolvanen JP,Hutri-Kähönen N,Kähönen M,Mäkynen H,Korpela R,Ruskoaho H,Karjala K,Pörsti I

    更新日期:1998-11-01 00:00:00

  • Potential role for adenosine in the pathogenesis of the vascular complications of hyperhomocysteinemia.

    abstract::Hyperhomocysteinemia is an independent risk factor for cardiovascular disease. Most previous investigations focused on the role of homocysteine as direct pathogenetic factor for these adverse vascular events. However, the exact pathophysiological mechanism is still unknown. In this review we discuss the hypothesis tha...

    journal_title:Cardiovascular research

    pub_type: 杂志文章,评审


    authors: Riksen NP,Rongen GA,Blom HJ,Russel FG,Boers GH,Smits P

    更新日期:2003-08-01 00:00:00