The posttranslational processing of ras p21 is critical for its stimulation of yeast adenylate cyclase.

abstract：:We have cloned and characterized the gene encoding the late histone H1-beta subtype from the sea urchin Strongylocentrotus purpuratus. The gene contains all of the upstream sequence homologies previously seen in late H1-gamma genes. The expression of H1-beta mRNA is coordinated with that of H1-gamma mRNA, and like H1-...

journal_title：Molecular and cellular biology

authors： Lai ZC,Childs G

更新日期：1988-04-01 00:00:00

abstract：:Telomere stabilization is critical for tumorigenesis. A number of tumors and cell lines use a recombination-based mechanism, alternative lengthening of telomeres (ALT), to maintain telomere repeat arrays. Current data suggest that the mutation of p53 facilitates the activation of this pathway. In addition to its funct...

journal_title：Molecular and cellular biology

authors： Razak ZR,Varkonyi RJ,Kulp-McEliece M,Caslini C,Testa JR,Murphy ME,Broccoli D

更新日期：2004-07-01 00:00:00

abstract：:Oncostatin M is a polypeptide cytokine, produced by normal and malignant hematopoietic cells, that has several in vitro activities, including the ability to inhibit growth of cultured carcinoma cells. Here we present a structural and functional comparison of two oncostatin M-related proteins (Mr 36,000 and 32,000) sec...

journal_title：Molecular and cellular biology

authors： Linsley PS,Kallestad J,Ochs V,Neubauer M

更新日期：1990-05-01 00:00:00

abstract：:Secretory proteins in eukaryotic cells are transported to the cell surface via the endoplasmic reticulum (ER) and the Golgi apparatus by membrane-bounded vesicles. We screened a collection of temperature-sensitive mutants of Saccharomyces cerevisiae for defects in ER-to-Golgi transport. Two of the genes identified in ...

journal_title：Molecular and cellular biology

authors： Chen EJ,Frand AR,Chitouras E,Kaiser CA

更新日期：1998-12-01 00:00:00

abstract：:Transcriptional regulation by nuclear receptors is controlled by the concerted action of coactivator and corepressor proteins. The product of the thyroid hormone-regulated mammalian gene hairless (Hr) was recently shown to function as a thyroid hormone receptor corepressor. Here we report that Hr acts as a potent repr...

journal_title：Molecular and cellular biology

authors： Moraitis AN,Giguère V,Thompson CC

更新日期：2002-10-01 00:00:00

abstract：:Mitogen activation of mRNA decay pathways likely involves specific endoribonucleases, such as G3BP, a phosphorylation-dependent endoribonuclease that associates with RasGAP in dividing but not quiescent cells. G3BP exclusively cleaves between cytosine and adenine (CA) after a specific interaction with RNA through the ...

journal_title：Molecular and cellular biology

authors： Tourrière H,Gallouzi IE,Chebli K,Capony JP,Mouaikel J,van der Geer P,Tazi J

更新日期：2001-11-01 00:00:00

abstract：:The fibroblast integrin alpha11beta1 is a key receptor for fibrillar collagens. To study the potential function of alpha11 in vivo, we generated a null allele of the alpha11 gene. Integrin alpha11(-/-) mice are viable and fertile but display dwarfism with increased mortality, most probably due to severely defective in...

journal_title：Molecular and cellular biology

authors： Popova SN,Barczyk M,Tiger CF,Beertsen W,Zigrino P,Aszodi A,Miosge N,Forsberg E,Gullberg D

更新日期：2007-06-01 00:00:00

abstract：:The ribonucleoprotein enzyme telomerase is a specialized reverse transcriptase that synthesizes telomeric DNA by copying a template sequence within the telomerase RNA. Here we analyze the actions of telomerase from Tetrahymena thermophila assembled in vivo with mutated or wild-type telomerase RNA to define further the...

journal_title：Molecular and cellular biology

authors： Gilley D,Blackburn EH

更新日期：1996-01-01 00:00:00

abstract：:We have analyzed the interleukin-4 (IL-4)-triggered mechanisms implicated in cell survival and show here that IL-4 deprivation induces apoptotic cell death but does not modulate Bcl-2 or Bcl-x expression. Since Bcl-x expression is insufficient to ensure cell survival in the absence of IL-4, we speculate that additiona...

journal_title：Molecular and cellular biology

authors： Rebollo A,Dumoutier L,Renauld JC,Zaballos A,Ayllón V,Martínez-A C

更新日期：2000-05-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the protein phosphatase type 1 (PP1)-binding protein Reg1 is required to maintain complete repression of ADH2 expression during growth on glucose. Surprisingly, however, mutant forms of the yeast PP1 homologue Glc7, which are unable to repress expression of another glucose-regulated gene, ...

journal_title：Molecular and cellular biology

authors： Dombek KM,Voronkova V,Raney A,Young ET

更新日期：1999-09-01 00:00:00

abstract：:SLP-76 is an adapter protein required for T-cell receptor (TCR) signaling. In particular, TCR-induced tyrosine phosphorylation and activation of phospholipase C-gamma1 (PLC-gamma1), and the resultant TCR-inducible gene expression, depend on SLP-76. Nonetheless, the mechanisms by which SLP-76 mediates PLC-gamma1 activa...

journal_title：Molecular and cellular biology

authors： Yablonski D,Kadlecek T,Weiss A

更新日期：2001-07-01 00:00:00

abstract：:The chaperone-related AAA ATPase Cdc48 (p97/VCP in higher eukaryotes) segregates ubiquitylated proteins for subsequent degradation by the 26S proteasome or for nonproteolytic fates. The specific outcome of Cdc48 activity is controlled by the evolutionary conserved cofactors Ufd2 and Ufd3, which antagonistically regula...

journal_title：Molecular and cellular biology

authors： Böhm S,Lamberti G,Fernández-Sáiz V,Stapf C,Buchberger A

更新日期：2011-04-01 00:00:00

abstract：:Studies ex vivo have shown that phosphoinositide 3-kinase (PI3K) activity is necessary but not sufficient for insulin-stimulated glucose uptake. Unexpectedly, mice lacking either of the PI3K regulatory subunits p85alpha or p85beta exhibit increased insulin sensitivity. The insulin hypersensitivity is particularly unex...

journal_title：Molecular and cellular biology

authors： Brachmann SM,Ueki K,Engelman JA,Kahn RC,Cantley LC

更新日期：2005-03-01 00:00:00

abstract：:The Ras pathway is critical for the development and function of T lymphocytes. The stimulation of this GTPase in T cells occurs primarily through the Vav1- and phospholipase C-gamma1-dependent activation of RasGRP1, a diacylglycerol-responsive Ras GDP/GTP exchange factor. Here, we show that a second exchange factor, R...

journal_title：Molecular and cellular biology

authors： Ruiz S,Santos E,Bustelo XR

更新日期：2007-12-01 00:00:00

abstract：:Fragments of 5'-flanking and noncoding exon I sequences of the human gastrin gene were analyzed in transient expression assays after transfection of a variety of cell lines with the pSVCAT vector system. In the presence of the simian virus 40 (SV40) enhancer, the gastrin gene fragment from nucleotides -250 to +57, rel...

journal_title：Molecular and cellular biology

authors： Theill LE,Wiborg O,Vuust J

更新日期：1987-12-01 00:00:00

abstract：:The production of tumor necrosis factor alpha (TNF-alpha), a key proinflammatory cytokine essential for the function of the immune system, is regulated at both the transcriptional and posttranscriptional levels. In this report, we focus on the interaction of TNF-alpha mRNA with macrophage proteins, likely mediators of...

journal_title：Molecular and cellular biology

authors： Hel Z,Skamene E,Radzioch D

更新日期：1996-10-01 00:00:00

abstract：:Upon the addition of different growth factors and cytokines, the Gab1 docking protein is tyrosine phosphorylated and in turn activates different signaling pathways. On the basis of the large body of evidence concerning cross talk between the signaling pathways activated by growth factors and oxidative stress, we decid...

journal_title：Molecular and cellular biology

authors： Holgado-Madruga M,Wong AJ

更新日期：2003-07-01 00:00:00

abstract：:RAD52 and RAD9 are required for the repair of double-strand breaks (DSBs) induced by physical and chemical DNA-damaging agents in Saccharomyces cerevisiae. Analysis of EcoRI endonuclease expression in vivo revealed that, in contrast to DSBs containing damaged or modified termini, chromosomal DSBs retaining complementa...

journal_title：Molecular and cellular biology

authors： Lewis LK,Kirchner JM,Resnick MA

更新日期：1998-04-01 00:00:00

abstract：:We have identified stable transcripts from the so-called nontranscribed spacer region (NTS) of the nuclear ribosomal DNA repeat in certain respiration-deficient strains of Saccharomyces cerevisiae. These RNAs, which are transcribed from the same strand as is the 37S rRNA precursor, are 500 to 800 nucleotides long and ...

journal_title：Molecular and cellular biology

authors： Parikh VS,Conrad-Webb H,Docherty R,Butow RA

更新日期：1989-05-01 00:00:00

abstract：:Revertants were selected from aryl hydrocarbon hydroxylase (AHH)-deficient recessive mutants belonging to three complementation groups and from a dominant mutant of the Hepa-1 cell line. The recessive mutants had low spontaneous reversion frequencies (less than 4 X 10(-7] that were increased by mutagenesis. The majori...

journal_title：Molecular and cellular biology

authors： Van Gurp JR,Hankinson O

更新日期：1984-08-01 00:00:00

abstract：:DNA end-labeling procedures were used to analyze both the frequency and distribution of DNA strand breaks in mammalian cells exposed or not to different types of DNA-damaging agents. The 3' ends were labeled by T4 DNA polymerase-catalyzed nucleotide exchange carried out in the absence or presence of Escherichia coli e...

journal_title：Molecular and cellular biology

authors： Legault J,Tremblay A,Ramotar D,Mirault ME

更新日期：1997-09-01 00:00:00

abstract：:Transcription activation of alpha-specific genes in Saccharomyces cerevisiae is regulated by two proteins, MCM1 and alpha 1, which bind to DNA sequences, called P'Q elements, found upstream of alpha-specific genes. Neither MCM1 nor alpha 1 alone binds efficiently to P'Q elements. Together, however, they bind cooperati...

journal_title：Molecular and cellular biology

authors： Hagen DC,Bruhn L,Westby CA,Sprague GF Jr

更新日期：1993-11-01 00:00:00

abstract：:The oncogene product of the avian sarcoma virus CT10, P47gag-crk, contains the SH2, SH2', and SH3 domains and binds proteins in a phosphotyrosine (ptyr)-dependent manner. In this study, we have determined the region of P47gag-crk essential for binding to ptyr-containing proteins. Mutant P47gag-crk proteins expressed i...

journal_title：Molecular and cellular biology

authors： Matsuda M,Mayer BJ,Hanafusa H

更新日期：1991-03-01 00:00:00

abstract：:Fibroblast growth factor 1 (FGF-1) is a powerful angiogenic factor whose gene structure contains four promoters, giving rise to a process of alternative splicing resulting in four mRNAs with alternative 5' untranslated regions (5' UTRs). Here we have identified, by using double luciferase bicistronic vectors, the pres...

journal_title：Molecular and cellular biology

authors： Martineau Y,Le Bec C,Monbrun L,Allo V,Chiu IM,Danos O,Moine H,Prats H,Prats AC

更新日期：2004-09-01 00:00:00

abstract：:The on/off state of the binary switch gene Sex-lethal (Sxl), which controls somatic sexual development in Drosophila melanogaster, is regulated at the level of alternative splicing. In males, in which the gene is off, the default splicing machinery produces nonfunctional mRNAs; in females, in which the gene is on, the...

journal_title：Molecular and cellular biology

authors： Horabin JI,Schedl P

更新日期：1993-12-01 00:00:00

abstract：:Although it is generally believed that the 60- and 81-base-pair (60/81-bp) repeats of the Xenopus laevis ribosomal DNA (rDNA) spacer are position-independent transcriptional enhancers, this has not been shown directly. We have now developed a critical assay which proves that the 60/81-bp repeats do, in fact, stimulate...

journal_title：Molecular and cellular biology

authors： Pape LK,Windle JJ,Mougey EB,Sollner-Webb B

更新日期：1989-11-01 00:00:00

abstract：:RNA editing in higher-plant chloroplasts involves C-to-U conversions at specific sites. Although in vivo analyses have been performed, little is known about the biochemical aspects of chloroplast editing reactions. Here we improved our original in vitro system and devised a procedure for preparing active chloroplast e...

journal_title：Molecular and cellular biology

authors： Miyamoto T,Obokata J,Sugiura M

更新日期：2002-10-01 00:00:00

abstract：:Multiple organ systems require epithelial barriers for normal function, and barrier loss is a hallmark of diseases ranging from inflammation to epithelial cancers. However, the molecular processes regulating epithelial barrier maturation are not fully elucidated. After contact, epithelial cells undergo size-reductive ...

journal_title：Molecular and cellular biology

authors： Bolinger MT,Ramshekar A,Waldschmidt HV,Larsen SD,Bewley MC,Flanagan JM,Antonetti DA

更新日期：2016-07-14 00:00:00

abstract：:The small subunit of ribonucleotide reductase in Saccharomyces cerevisiae (RNR2) was induced 3- to 20-fold by a variety of DNA-damaging agents. Induction of the RNR2 transcript by at least one of these agents, methyl methanesulfonate, did not require protein synthesis. To identify sequences involved in the regulation ...

journal_title：Molecular and cellular biology

authors： Hurd HK,Roberts JW

更新日期：1989-12-01 00:00:00

abstract：:A mouse adenine phosphoribosyltransferase (aprt) pseudogene that had previously been recovered from a BALB/c sperm DNA library possessed several unusual features. Its nucleotide sequence, like that of other processed pseudogenes, was colinear with its corresponding mRNA, but it was truncated at its 3' end and lacked a...

journal_title：Molecular and cellular biology

authors： Dush MK,Tischfield JA,Khan SA,Feliciano E,Sikela JM,Kozak CA,Stambrook PJ

更新日期：1986-12-01 00:00:00