Irradiation-induced expression of O6-methylguanine-DNA methyltransferase in mammalian cells.

Abstract:

:O6-Methylguanine-DNA methyltransferase (MGMT) is a DNA repair protein which plays an important role in chemotherapy, mutagenesis, and carcinogenesis. The specific activity of MGMT in female rat liver can be induced by approximately 20-fold by treatment of the rats with gamma-irradiation. Maximum response occurred 48 h after 15 Gy irradiation. MGMT levels in male rats were induced by only 3-fold. MGMT activity was also induced by irradiation of rat hepatoma H4IIE cells with a 3-fold increase noted after treatment with 3 Gy. Northern analysis and nuclear run-on assays indicated that the induction of MGMT was regulated at the transcriptional level. The radiation-mediated increase in MGMT was blocked by H7, a protein kinase inhibitor, but not by H89, an inhibitor of protein kinase A. Hydroxyl radicals may play a role in the induction mechanism since dimethyl sulfoxide, a radical scavenger, blocked the radiation-mediated increase in MGMT. MGMT activity was also increased by treatment of the cells with H2O2, in accordance with the involvement of activated oxygen species in the induction of MGMT. Finally, the addition of cycloheximide, an inhibitor of protein synthesis, prior to but not after irradiation, abolished the increase in MGMT activity.

journal_name

Cancer Res

journal_title

Cancer research

authors

Chan CL,Wu Z,Eastman A,Bresnick E

subject

Has Abstract

pub_date

1992-04-01 00:00:00

pages

1804-9

issue

7

eissn

0008-5472

issn

1538-7445

journal_volume

52

pub_type

杂志文章
  • Regulation of carbonyl-reducing enzymes in rat liver by chemoprotectors.

    abstract::Feeding rats on diets containing the synthetic antioxidants ethoxyquin, butylated hydroxyanisole, and oltipraz results in 15-, 9-, and 6-fold increases, respectively, in the hepatic levels of aflatoxin B1-dialdehyde reductase (AFAR) protein. By contrast, treatment of rats with either of the inducing agents phenobarbit...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Ellis EM,Judah DJ,Neal GE,O'Connor T,Hayes JD

    更新日期:1996-06-15 00:00:00

  • Increased synthesis of carbamoyl-phosphate synthase II (EC 6.3.5.5) in hepatoma 3924A.

    abstract::Carbamoyl-phosphate synthase II (glutamine-hydrolyzing) (EC 6.3.5.5) (synthase II) is the first and rate-limiting enzyme in the de novo UTP biosynthetic pathway. Leucine pulse-labeling in the rat demonstrated that in the rapidly proliferating hepatoma 3924A the ratio of radioactivity of synthase II to that of total cy...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Reardon MA,Weber G

    更新日期:1986-07-01 00:00:00

  • Cellular and serum involvement in protection against Friend leukemia virus.

    abstract::Treatment of mice with the immunomodulator pyran copolymer inhibited leukemogenesis produced by Friend leukemia virus (FLV) complex, as evidenced by inhibition of the spleen focus-forming virus and lymphatic leukemia virus, as well as by a significant decrease in splenomegaly. In this report we present data suggesting...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Schuller GB,Morahan PS

    更新日期:1977-11-01 00:00:00

  • Aspirin toxicity for human colonic tumor cells results from necrosis and is accompanied by cell cycle arrest.

    abstract::Chemoprevention of colorectal cancer using aspirin has been demonstrated in rodents and has been suggested by data from epidemiological studies. The mechanism that accounts for this prevention is unknown, but it is thought to relate to an irreversible inhibition of cyclooxygenase and, subsequently, prostaglandin produ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Subbegowda R,Frommel TO

    更新日期:1998-07-01 00:00:00

  • Inhibition of mouse alpha/beta-interferon of the multiplication of alpha/beta-interferon-resistant Friend erythroleukemia cells cocultured with mouse hepatocytes.

    abstract::Administration of alpha/beta-interferon (IFN) exerts a marked antitumor effect in DBA/2 mice given injections i.v. of large numbers of IFN-alpha/beta-resistant erythroleukemia cells (FLC). To investigate the possible mechanisms of FLC tumor inhibition in the liver of interferon-treated mice, we developed an in vitro m...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Yasui H,Proietti E,Vignaux F,Eid P,Gresser I

    更新日期:1990-06-15 00:00:00

  • Immunolymphoscintigraphy for the detection of lymph node metastases from breast cancer.

    abstract::The presence of metastases in the regional lymph nodes is the major prognostic factor in breast cancer in the absence of overt distant metastases and is also an important indicator of the need for adjuvant therapy in "early" breast cancer. Currently, the accurate assessment of axillary lymph node status requires axill...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Tjandra JJ,Russell IS,Collins JP,Andrews JT,Lichtenstein M,Binns D,McKenzie IF

    更新日期:1989-03-15 00:00:00

  • ADP-ribosylation factor 6 regulates glioma cell invasion through the IQ-domain GTPase-activating protein 1-Rac1-mediated pathway.

    abstract::A common pathobiological feature of malignant gliomas is the insidious infiltration of single tumor cells into the brain parenchyma, rendering these deadly tumors virtually incurable with available therapies. In this study, we report that ADP-ribosylation factor 6 (ARF6), a Ras superfamily small GTPase, is abundantly ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-08-2110

    authors: Hu B,Shi B,Jarzynka MJ,Yiin JJ,D'Souza-Schorey C,Cheng SY

    更新日期:2009-02-01 00:00:00

  • Absence of genetic alteration at codon 531 of the human c-src gene in 479 advanced colorectal cancers from Japanese and Caucasian patients.

    abstract::Activation of c-src, a cellular human gene homologous in sequence to the v-src gene of Rous sarcoma virus, had been thought to play an important role in the progression of several types of human cancers, without having undergone any genetic changes. However, recently truncating mutations at codon 531 of the c-src gene...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Daigo Y,Furukawa Y,Kawasoe T,Ishiguro H,Fujita M,Sugai S,Nakamori S,Liefers GJ,Tollenaar RA,van de Velde CJ,Nakamura Y

    更新日期:1999-09-01 00:00:00

  • Serum micronutrients and the subsequent risk of oral and pharyngeal cancer.

    abstract::To investigate the relationship between serum micronutrients and the subsequent risk of oral and pharyngeal cancer, a nested case-control study was conducted within a cohort of 25,802 adults in Washington County, MD, whose blood samples were collected in 1974 and stored at -70 degrees C for subsequent assays. The seru...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Zheng W,Blot WJ,Diamond EL,Norkus EP,Spate V,Morris JS,Comstock GW

    更新日期:1993-02-15 00:00:00

  • Increased urinary excretion of 2-hydroxyestrone but not 16alpha-hydroxyestrone in premenopausal women during a soya diet containing isoflavones.

    abstract::Asian diets high in soy are associated with lower risk for breast cancer compared with Western diets. Moreover, higher levels of two putative carcinogenic metabolites of 17beta-estradiol, 4- and 16alpha-hydroxyestrogen, and lower amounts of anticarcinogenic metabolites, 2-hydroxyestrogens, have been associated with gr...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Lu LJ,Cree M,Josyula S,Nagamani M,Grady JJ,Anderson KE

    更新日期:2000-03-01 00:00:00

  • Signal transducer and activator of transcription 1 activation in endothelial cells is a negative regulator of angiogenesis.

    abstract::To determine the role of the transcription factor signal transducer and activator of transcription (STAT) 1 on endothelial cell function, human umbilical vein endothelial cells (HUVEC) were treated with IFN-gamma, a potent activator of STAT1. IFN-gamma inhibited cell growth and tube formation of HUVECs. Although the p...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-05-3612

    authors: Battle TE,Lynch RA,Frank DA

    更新日期:2006-04-01 00:00:00

  • Preclinical study of sequential tumor necrosis factor and interleukin 2 in the treatment of spontaneous canine neoplasms.

    abstract::Recombinant human tumor necrosis factor and recombinant human interleukin 2 were administered in a sequential schedule to 30 dogs with a variety of spontaneous neoplasms. Dose escalation of both drugs was performed, and a maximally tolerated dose of recombinant human tumor necrosis factor of 125 mg/m2 i.v. for 3 days,...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Moore AS,Theilen GH,Newell AD,Madewell BR,Rudolf AR

    更新日期:1991-01-01 00:00:00

  • Amplification, rearrangements, and enhanced expression of c-myc in chemically induced rat liver tumors in vivo and in vitro.

    abstract::c-myc expression, amplification, and rearrangement were studied in neoplastic hepatic nodules of rats after diethylnitrosamine treatment, in hepatocellular carcinomas induced by N-methyl-N-nitrosourea, and in tumorigenic liver cell lines derived from rat hepatocytes transformed by diethylnitrosamine in vivo. Steady-st...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Suchy BK,Sarafoff M,Kerler R,Rabes HM

    更新日期:1989-12-01 00:00:00

  • Snail1-expressing fibroblasts in the tumor microenvironment display mechanical properties that support metastasis.

    abstract::Crosstalk between tumor and stromal cells in the tumor microenvironment alter its properties in ways that facilitate the invasive behavior of tumor cells. Here, we demonstrate that cancer-associated fibroblasts (CAF) increase the stiffness of the extracellular matrix (ECM) and promote anisotropic fiber orientation, tw...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-14-1903

    authors: Stanisavljevic J,Loubat-Casanovas J,Herrera M,Luque T,Peña R,Lluch A,Albanell J,Bonilla F,Rovira A,Peña C,Navajas D,Rojo F,García de Herreros A,Baulida J

    更新日期:2015-01-15 00:00:00

  • Tumor genotype-specific growth inhibition in vivo by antisense oligonucleotides against a polymorphic site of the large subunit of human RNA polymerase II.

    abstract::Loss of heterozygosity (LOH) reduces genes to hemizygosity in cancer cells and presents an absolute difference between normal and cancer cells. The regions of LOH are usually much larger than the tumor suppressor gene, which is lost, and are expected to contain genes that are essential for cell survival. Single nucleo...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Fluiter K,ten Asbroek AL,van Groenigen M,Nooij M,Aalders MC,Baas F

    更新日期:2002-04-01 00:00:00

  • Antibody microarray profiling reveals individual and combined serum proteins associated with pancreatic cancer.

    abstract::We used antibody microarrays to probe the associations of multiple serum proteins with pancreatic cancer and to explore the use of combined measurements for sample classification. Serum samples from pancreatic cancer patients (n = 61), patients with benign pancreatic disease (n = 31), and healthy control subjects (n =...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-05-1436

    authors: Orchekowski R,Hamelinck D,Li L,Gliwa E,vanBrocklin M,Marrero JA,Vande Woude GF,Feng Z,Brand R,Haab BB

    更新日期:2005-12-01 00:00:00

  • Pharmacology and neurotoxicity of cis-diamminedichloroplatinum, bleomycin, 5-fluorouracil, and cyclophosphamide administration following osmotic blood-brain barrier modification.

    abstract::cis-Platinum, bleomycin, 5-fluorouracil, and cyclophosphamide were administered to rodent and canine models of osmotic blood-brain barrier modification to evaluate the relationship between tissue drug concentration in brain and the physiological and neuropathological effects of the drug. The toxicity studies were carr...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Neuwelt EA,Barnett PA,Glasberg M,Frenkel EP

    更新日期:1983-11-01 00:00:00

  • Comparative expression analysis reveals lineage relationships between human and murine gliomas and a dominance of glial signatures during tumor propagation in vitro.

    abstract::Brain tumors are thought to originate from stem/progenitor cell populations that acquire specific genetic mutations. Although current preclinical models have relevance to human pathogenesis, most do not recapitulate the histogenesis of the human disease. Recently, a large series of human gliomas and medulloblastomas w...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-13-1299

    authors: Henriquez NV,Forshew T,Tatevossian R,Ellis M,Richard-Loendt A,Rogers H,Jacques TS,Reitboeck PG,Pearce K,Sheer D,Grundy RG,Brandner S

    更新日期:2013-09-15 00:00:00

  • Adaptive Upregulation of EGFR Limits Attenuation of Tumor Growth by Neutralizing IL6 Antibodies, with Implications for Combined Therapy in Ovarian Cancer.

    abstract::Excess production of the proinflammatory IL6 has both local and systemic tumor-promoting activity in many cancers, including ovarian cancer. However, treatment of advanced ovarian cancer patients with a neutralizing IL6 antibody yielded little efficacy in a previous phase II clinical trial. Here, we report results tha...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-14-1801

    authors: Milagre CS,Gopinathan G,Everitt G,Thompson RG,Kulbe H,Zhong H,Hollingsworth RE,Grose R,Bowtell DD,Hochhauser D,Balkwill FR

    更新日期:2015-04-01 00:00:00

  • Immunoassay of serum galactosyltransferase isoenzyme II in cancer patients and control subjects.

    abstract::Serum galactosyltransferase isoenzyme II (GT-II) was assayed in 409 coded serum samples obtained from the National Cancer Institute Tumor Serum Bank using a monoclonal antibody-based immunoassay. The serum panel consisted of samples from patients with confirmed, metastatic ovarian, breast, stomach, esophageal, pancrea...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Uemura M,Winant RC,Brandt AE

    更新日期:1988-09-15 00:00:00

  • Neuropeptide Y expression in the developing adrenal gland and in childhood neuroblastoma tumors.

    abstract::Neuropeptide Y (NPY) expression is limited to tissues of the central and peripheral nervous system. In the adrenal gland, NPY is found in a subset of cells of the adrenal medulla. Using in situ hybridization analysis, NPY mRNA expression was characterized during human fetal adrenal medullary development. We found a bi...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Cohen PS,Cooper MJ,Helman LJ,Thiele CJ,Seeger RC,Israel MA

    更新日期:1990-09-15 00:00:00

  • CpG island methylator phenotype is a strong determinant of poor prognosis in neuroblastomas.

    abstract::Neuroblastoma, one of the most common pediatric solid tumors, is characterized by two extreme disease courses, spontaneous regression and life-threatening progression. Here, we conducted a genome-wide search for differences in DNA methylation that distinguish between neuroblastomas of the two types. Three CpG islands ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Abe M,Ohira M,Kaneda A,Yagi Y,Yamamoto S,Kitano Y,Takato T,Nakagawara A,Ushijima T

    更新日期:2005-02-01 00:00:00

  • Influence of esophagojejunostomy on the induction of adenocarcinoma of the distal esophagus in Sprague-Dawley rats by subcutaneous injection of 2,6-dimethylnitrosomorpholine.

    abstract::To study the influence of reflux esophagitis on the carcinogenic response of 2,6-dimethylnitrosomorpholine (2,6-DMNM), an experiment was designed, composed of 6 groups of 8-week-old Sprague-Dawley rats, each consisting of 20 males and 20 females. Group 1 served as untreated controls. All animals of groups 2, 4, and 6 ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Pera M,Cardesa A,Bombi JA,Ernst H,Pera C,Mohr U

    更新日期:1989-12-01 00:00:00

  • Integrin alpha v beta 3 controls activity and oncogenic potential of primed c-Src.

    abstract::Increased activity of the proto-oncogene c-Src and elevated levels of integrin alpha(v)beta(3) are found in melanomas and multiple carcinomas. Regulation of c-Src involves "priming" through disruption of intramolecular interactions followed by "activation" through phosphorylation in the kinase domain. Interactions wit...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-06-3654

    authors: Huveneers S,van den Bout I,Sonneveld P,Sancho A,Sonnenberg A,Danen EH

    更新日期:2007-03-15 00:00:00

  • Interleukin 6 gene transfection into Lewis lung carcinoma tumor cells suppresses the malignant phenotype and confers immunotherapeutic competence against parental metastatic cells.

    abstract::To investigate the influence of interleukin 6 (IL-6) production on malignancy of tumor cells we transfected cells of the high-metastatic, low-immunogenic D122 clone of the Lewis lung carcinoma with a mammalian expression vector containing the human IL-6 complementary DNA. In vitro, IL-6 positive transfectants showed g...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Porgador A,Tzehoval E,Katz A,Vadai E,Revel M,Feldman M,Eisenbach L

    更新日期:1992-07-01 00:00:00

  • Phospholipase A2 activation and autoinduction of tumor necrosis factor gene expression by tumor necrosis factor.

    abstract::Tumor necrosis factor (TNF) acts via a cell surface receptor to induce a variety of cellular events including cytolysis, differentiation, and mitogenesis. The mechanisms underlying the cell specific actions of TNF are not known. In the present study, postreceptor events associated with the autoinduction of TNF express...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Spriggs DR,Sherman ML,Imamura K,Mohri M,Rodriguez C,Robbins G,Kufe DW

    更新日期:1990-11-15 00:00:00

  • MSH2 Dysregulation Is Triggered by Proinflammatory Cytokine Stimulation and Is Associated with Liver Cancer Development.

    abstract::Inflammation predisposes to tumorigenesis in various organs by potentiating a susceptibility to genetic aberrations. The mechanism underlying the enhanced genetic instability through chronic inflammation, however, is not clear. Here, we demonstrated that TNFα stimulation induced transcriptional downregulation of MSH2,...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:10.1158/0008-5472.CAN-15-2926

    authors: Eso Y,Takai A,Matsumoto T,Inuzuka T,Horie T,Ono K,Uemoto S,Lee K,Edelmann W,Chiba T,Marusawa H

    更新日期:2016-08-01 00:00:00

  • Allelic loss at the predisposing gene locus in spontaneous and chemically induced renal cell carcinomas in the Eker rat.

    abstract::Hereditary renal carcinoma (RC) in the rat, originally reported by Eker in 1954, is an example of a Mendelian dominant predisposition to a specific cancer in an experimental animal. We previously reported that ionizing radiation induces additional tumors in a linear dose-response relationship, suggesting that in heter...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Kubo Y,Mitani H,Hino O

    更新日期:1994-05-15 00:00:00

  • Cytotoxic efficacy of 9-nitrocamptothecin in the treatment of human malignant melanoma cells in vitro.

    abstract::In a recent study, we showed that the plant alkaloid camptothecin (CPT) and its derivatives 9-nitro-CPT (9NC) and 9-amino-CPT (9AC) inhibit growth of both human melanocytes (MEL cells) and their malignant counterparts, malignant melanoma (BRO) cells in vitro. This growth inhibition was accompanied by an increase in th...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Pantazis P,Early JA,Mendoza JT,DeJesus AR,Giovanella BC

    更新日期:1994-02-01 00:00:00

  • Neopterin formation and tryptophan degradation by a human myelomonocytic cell line (THP-1) upon cytokine treatment.

    abstract::Determination of neopterin [D-erythro-6-(1',2',3'-trihydroxypropyl)pterin] in body fluids is a powerful diagnostic tool in a variety of diseases in which activation of cellular immune mechanisms is involved, such as certain malignancies, allograft rejection, and autoimmune and infectious diseases. In vitro, neopterin ...

    journal_title:Cancer research

    pub_type: 杂志文章

    doi:

    authors: Werner-Felmayer G,Werner ER,Fuchs D,Hausen A,Reibnegger G,Wachter H

    更新日期:1990-05-15 00:00:00