The amino-terminal half of pp59c-fyn contains sequences necessary for formation of a 75 kDa form and also repressive elements absent in pp60c-src.

Abstract:

:Members of the src family of tyrosine kinases are composed of amino acid sequences which can be divided into three regions: the unique amino-terminal 80 residues; the next 180 residues of conserved but non-catalytic sequence; and the catalytic carboxy-terminal half of the molecule. To characterize the elements that regulate the catalytic and transforming activities of two members of this family, pp59c-fyn and pp60c-src, we generated six chimeric proteins by interchanging the three regions of the 531F mutant of pp59c-fyn and of the 527F mutant of pp60c-src. Our data indicate that substituting all or part of the amino-terminal 263 residues of pp59c-fyn for those of 527F inhibited the kinase and transforming activities of 527F. Conversely, substituting the amino-terminal half of pp60c-src for that of 531F resulted in an increase in the transforming potential of 531F. These results suggest that the amino-terminal half of pp59c-fyn contains elements which act to suppress the catalytic and transforming activities of the enzyme and that these suppressive elements are either absent or inactive in pp60c-src. These differences argue that the src family of tyrosine kinases are regulated differently in the cell. In vitro translation of some of the chimeras in rabbit reticulocyte lysates generated a 75 kDa protein in addition to the expected 59 kDa product. This 75 kDa species is analogous to the p75 protein previously detected in wild-type pp59c-fyn translation products. Interestingly, formation of p75 required the presence of DNA sequences encoding the unique amino-terminal residues of pp59c-fyn.

journal_name

Oncogene

journal_title

Oncogene

authors

Espino PC,Chou WY,Smith AE,Cheng SH

subject

Has Abstract

pub_date

1992-02-01 00:00:00

pages

317-22

issue

2

eissn

0950-9232

issn

1476-5594

journal_volume

7

pub_type

杂志文章

相关文献

ONCOGENE文献大全
  • Interleukin-17 promotes prostate cancer via MMP7-induced epithelial-to-mesenchymal transition.

    abstract::Chronic inflammation has been associated with a variety of human cancers including prostate cancer. Interleukin-17 (IL-17) is a critical pro-inflammatory cytokine, which has been demonstrated to promote development of prostate cancer, colon cancer, skin cancer, breast cancer, lung cancer and pancreas cancer. IL-17 pro...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/onc.2016.240

    authors: Zhang Q,Liu S,Parajuli KR,Zhang W,Zhang K,Mo Z,Liu J,Chen Z,Yang S,Wang AR,Myers L,You Z

    更新日期:2017-02-02 00:00:00

  • Up-regulation of hypoxia-inducible factors HIF-1alpha and HIF-2alpha under normoxic conditions in renal carcinoma cells by von Hippel-Lindau tumor suppressor gene loss of function.

    abstract::Hypoxia induces transcription of a range of physiologically important genes including erythropoietin and vascular endothelial growth factor. The transcriptional activation is mediated by the hypoxia-inducible factor-1 (HIF-1), a heterodimeric member of the basic helix-loop-helix PAS family, composed of alpha and beta ...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1203938

    authors: Krieg M,Haas R,Brauch H,Acker T,Flamme I,Plate KH

    更新日期:2000-11-16 00:00:00

  • E2F regulates DDB2: consequences for DNA repair in Rb-deficient cells.

    abstract::DDB2, a gene mutated in XPE patients, is involved in global genomic repair especially the repair of cyclobutane pyrimidine dimers (CPDs), and is regulated by p53 in human cells. We show that DDB2 is expressed in mouse tissues and demonstrate, using primary mouse epithelial cells, that mouse DDB2 is regulated by E2F tr...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1210151

    authors: Prost S,Lu P,Caldwell H,Harrison D

    更新日期:2007-05-24 00:00:00

  • Alterations in the common fragile site gene Parkin in ovarian and other cancers.

    abstract::The cloning and characterization of the common fragile site (CFS) FRA6E (6q26) identified Parkin, the gene involved in the pathogenesis of many cases of juvenile, early-onset and, rarely, late-onset Parkinson's disease, as the third large gene to be localized within a large CFS. Initial analyses of Parkin indicated th...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1207072

    authors: Denison SR,Wang F,Becker NA,Schüle B,Kock N,Phillips LA,Klein C,Smith DI

    更新日期:2003-11-13 00:00:00

  • The HMG-box transcription factor HBP1 is targeted by the pocket proteins and E1A.

    abstract::A yeast two-hybrid screen has identified HBP1 as a transcription factor capable of interacting with the pocket protein family. We show that HBP1 can interact with one of these, RB, both in vitro and in mammalian cells. Two distinct RB binding sites are present within HBP1--a high affinity binding site, mediated by an ...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1201243

    authors: Lavender P,Vandel L,Bannister AJ,Kouzarides T

    更新日期:1997-06-05 00:00:00

  • PHLPP2 stabilization by p27 mediates its inhibition of bladder cancer invasion by promoting autophagic degradation of MMP2 protein.

    abstract::Pleckstrin homology domain leucine-rich repeat protein phosphatase 2 (PHLPP2) is a tumor suppressor that catalyzes the de-phosphorylation of the AGC kinases, while p27 acts as a tumor suppressor that regulates cell cycle, apoptosis, and cell motility. Our previous studies have identified that PHLPP2 participates in in...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/s41388-018-0374-1

    authors: Peng M,Wang J,Zhang D,Jin H,Li J,Wu XR,Huang C

    更新日期:2018-10-01 00:00:00

  • AP-1 complexes containing cJun and JunB cause cellular transformation of Rat1a fibroblasts and share transcriptional targets.

    abstract::To investigate the role of individual Jun proteins in cell growth and transformation, we have used a doxycycline-inducible retroviral vector to regulate their expression in rat fibroblasts. AP-1 complexes enriched with cJun and JunB result in morphological alterations and anchorage-independent cell growth consistent w...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1206644

    authors: Leaner VD,Kinoshita I,Birrer MJ

    更新日期:2003-08-28 00:00:00

  • EBP1 is a nucleolar growth-regulating protein that is part of pre-ribosomal ribonucleoprotein complexes.

    abstract::EBP1 was identified as a protein that interacts with the ErbB-3 receptor and possibly contributes to transducing growth regulatory signals. The existence of EBP1 homologs across species from simple eukaryotes to humans and its wide tissue expression pattern suggest that EBP1 acts as a general signaling molecule. We pr...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1207579

    authors: Squatrito M,Mancino M,Donzelli M,Areces LB,Draetta GF

    更新日期:2004-05-27 00:00:00

  • A clinical drug library screen identifies clobetasol propionate as an NRF2 inhibitor with potential therapeutic efficacy in KEAP1 mutant lung cancer.

    abstract::The Kelch-like ECH-associated protein 1 (KEAP1)-nuclear factor E2-related factor 2 (NRF2)pathway has a central role in cellular antioxidant defense. NRF2 activation due to KEAP1 or NRF2 mutations occurs frequently in many cancers, suggesting that NRF2 inhibition could be a promising therapeutic strategy. However, no p...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/onc.2017.153

    authors: Choi EJ,Jung BJ,Lee SH,Yoo HS,Shin EA,Ko HJ,Chang S,Kim SY,Jeon SM

    更新日期:2017-09-14 00:00:00

  • Cross-talk between Akt, p53 and Mdm2: possible implications for the regulation of apoptosis.

    abstract::The p53 tumor suppressor protein and the Akt/PKB kinase play important roles in the transduction of pro-apoptotic and anti-apoptotic signals, respectively. We provide evidence that conflicting signals transduced by Akt and p53 are integrated via negative feedback between the two pathways. On the one hand, the combinat...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1205181

    authors: Gottlieb TM,Leal JF,Seger R,Taya Y,Oren M

    更新日期:2002-02-14 00:00:00

  • Epstein-barr virus transformation: involvement of latent membrane protein 1-mediated activation of NF-kappaB.

    abstract::Epstein-Barr virus (EBV) transforms resting primary human B lymphocytes into indefinitely proliferating lymphoblastoid cell lines in vitro and is associated with several human malignancies in vivo. Recombinant EBV genetic analyses combined with in vitro B lymphocyte transformation assays demonstrate that latent infect...

    journal_title:Oncogene

    pub_type: 杂志文章,评审

    doi:10.1038/sj.onc.1203217

    authors: Cahir McFarland ED,Izumi KM,Mosialos G

    更新日期:1999-11-22 00:00:00

  • Mechanism and functional consequences of loss of FOXO1 expression in endometrioid endometrial cancer cells.

    abstract::The forkhead transcription factor FOXO1, a downstream target of phosphatidylinositol-3-kinase/Akt signalling pathway, regulates cyclic differentiation and apoptosis in normal endometrium, but its role in endometrial carcinogenesis is unknown. Screening of endometrial cancer cell lines demonstrated that FOXO1 is expres...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1210626

    authors: Goto T,Takano M,Albergaria A,Briese J,Pomeranz KM,Cloke B,Fusi L,Feroze-Zaidi F,Maywald N,Sajin M,Dina RE,Ishihara O,Takeda S,Lam EW,Bamberger AM,Ghaem-Maghami S,Brosens JJ

    更新日期:2008-01-03 00:00:00

  • Deletion mapping implicates two tumor suppressor genes on chromosome 8p in the development of bladder cancer.

    abstract::Chromosome 8 loss of heterozygosity (LOH) in cancer of the urinary bladder is associated with high tumour grade and stage. We have screened 193 cases of transitional cell carcinoma (TCC) of the bladder using 30 microsatellite polymorphisms on chromosome 8. Forty three cases (22%) showed LOH on 8p, the majority of whic...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:

    authors: Takle LA,Knowles MA

    更新日期:1996-03-07 00:00:00

  • mafA, a novel member of the maf proto-oncogene family, displays developmental regulation and mitogenic capacity in avian neuroretina cells.

    abstract::Transcription factors of the Maf proto-oncogene family have been shown to participate in the regulation of several differentiation specific genes. We previously reported that a member(s) of this family is involved in the regulation of the neuroretina specific gene, QR1, through a promoter region, designated the A box,...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1201898

    authors: Benkhelifa S,Provot S,Lecoq O,Pouponnot C,Calothy G,Felder-Schmittbuhl MP

    更新日期:1998-07-16 00:00:00

  • Molecular organization and differential polyadenylation sites of the human ETS2 gene.

    abstract::The human ETS2 gene is a member of the ets gene family (ETS1, ETS2, ERG, ELK1 and ELK2) with amino acid similarity to the v-ets oncogene of the avian leukemia virus, E26. The ETS2 gene is composed of 10 exons, nine of which contribute to the open reading frame encoding 469 amino acids. The ETS2 gene directs the synthe...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:

    authors: Watson DK,Mavrothalassitis GJ,Jorcyk CL,Smyth FE,Papas TS

    更新日期:1990-10-01 00:00:00

  • Immortalization and transformation of human fibroblasts by regulated expression of polyoma virus T antigens.

    abstract::We have established conditions for the immortalization of human fibroblasts by the large T antigen of the rodent virus polyoma. This allows the mechanism of immortalization to be studied, without interference by transformation events, in cells with relatively stable chromosomes. Large T antigen could immortalize human...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:

    authors: Strauss M,Hering S,Lubbe L,Griffin BE

    更新日期:1990-08-01 00:00:00

  • The protein product of the c-cbl oncogene rapidly complexes with the EGF receptor and is tyrosine phosphorylated following EGF stimulation.

    abstract::The cbl oncogene was first identified as part of a transforming retrovirus which arose in a mouse pre-B cell lymphoma. Its protein product, p120cbl, is cytoplasmic and has several distinctive domains including a highly basic region, a RING finger motif and a large proline-rich domain. A mutation to cbl in the 70Z/3 pr...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:

    authors: Bowtell DD,Langdon WY

    更新日期:1995-10-19 00:00:00

  • Redistribution of CD95, DR4 and DR5 in rafts accounts for the synergistic toxicity of resveratrol and death receptor ligands in colon carcinoma cells.

    abstract::The natural phytoalexin resveratrol (3, 5, 4'-trihydroxystilbene) exhibits both chemopreventive and antitumor activities through a variety of mechanisms. We have shown previously that resveratrol-induced apoptosis of a human colon cancer cell line involved the redistribution of CD95 (Fas/Apo-1) into lipid rafts. Here,...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1208086

    authors: Delmas D,Rébé C,Micheau O,Athias A,Gambert P,Grazide S,Laurent G,Latruffe N,Solary E

    更新日期:2004-11-25 00:00:00

  • Regulation of Pax3 transcriptional activity by SUMO-1-modified PML.

    abstract::Pax3 is an evolutionarily conserved transcription factor that plays a major role in a variety of developmental processes. Mutations in Pax3 lead to severe malformations as seen in human Waardenburg syndrome and in the Splotch mutant mice. The transcriptional activity of Pax3 was recently shown to be repressed by Daxx ...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1204063

    authors: Lehembre F,Müller S,Pandolfi PP,Dejean A

    更新日期:2001-01-04 00:00:00

  • RARgamma acts as a tumor suppressor in mouse keratinocytes.

    abstract::All-trans retinoic acid (RA), the principle biologically active form of vitamin A, is essential for many developmental process as well as homeostasis in the adult. Many lines of evidence also suggest that RA, acting through the RA receptors (RARs), can also suppress growth of tumors of diverse origin. To assess direct...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1207682

    authors: Chen CF,Goyette P,Lohnes D

    更新日期:2004-07-08 00:00:00

  • Copine-I represses NF-kappaB transcription by endoproteolysis of p65.

    abstract::Nuclear factor-kappaB (NF-kappaB) is a dynamic transcription factor that regulates important biological processes involved in cancer initiation and progression. Identifying regulators that control the half-life of NF-kappaB is important to understanding molecular processes that control the duration of transcriptional ...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1211030

    authors: Ramsey CS,Yeung F,Stoddard PB,Li D,Creutz CE,Mayo MW

    更新日期:2008-06-05 00:00:00

  • p53 inhibits mRNA 3' processing through its interaction with the CstF/BARD1 complex.

    abstract::The mechanisms involved in the p53-dependent control of gene expression following DNA damage have not been completely elucidated. Here, we show that the p53 C terminus associates with factors that are required for the ultraviolet (UV)-induced inhibition of the mRNA 3' cleavage step of the polyadenylation reaction, suc...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/onc.2011.29

    authors: Nazeer FI,Devany E,Mohammed S,Fonseca D,Akukwe B,Taveras C,Kleiman FE

    更新日期:2011-07-07 00:00:00

  • Mouse Sin3A interacts with and can functionally substitute for the amino-terminal repression of the Myc antagonist Mxi1.

    abstract::Mxi1 is a basic region helix-loop-helix leucine zipper (bHLH/LZ) protein that, in association with Max, antagonizes Myc oncogenic activities. A possible mechanistic basis for Mxi1-mediated repression was provided by the recent demonstration that the repressive potential of Mxi1 correlates with its ability to physicall...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:

    authors: Rao G,Alland L,Guida P,Schreiber-Agus N,Chen K,Chin L,Rochelle JM,Seldin MF,Skoultchi AI,DePinho RA

    更新日期:1996-03-07 00:00:00

  • Nerve growth factor induces survival and differentiation through two distinct signaling cascades in PC12 cells.

    abstract::Nerve growth factor induces differentiation and survival of rat PC12 pheochromocytoma cells. The activation of the erk cascade has been implicated in transducing the multitude of signals induced by NGF. In order to explore the role of this signaling cascade in NGF mediated survival, differentiation and proliferation, ...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1202524

    authors: Klesse LJ,Meyers KA,Marshall CJ,Parada LF

    更新日期:1999-03-25 00:00:00

  • Tie2-mediated multidrug resistance in malignant gliomas is associated with upregulation of ABC transporters.

    abstract::Resistance and relapse are still primary causes that result in poor effectiveness of chemotherapy in malignant gliomas. Therefore, development of new therapeutic strategies requires the identification of key molecular pathways regulating chemoresistance. We previously found that abnormal high expression of the Tie2 re...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/onc.2009.103

    authors: Martin V,Xu J,Pabbisetty SK,Alonso MM,Liu D,Lee OH,Gumin J,Bhat KP,Colman H,Lang FF,Fueyo J,Gomez-Manzano C

    更新日期:2009-06-18 00:00:00

  • Targeted expression of HGF/SF in mouse mammary epithelium leads to metastatic adenosquamous carcinomas through the activation of multiple signal transduction pathways.

    abstract::Overexpression of hepatocyte growth factor (HGF), also called scatter factor (SF), and its receptor c-Met are associated with poor prognosis for cancer patients. In particular, breast cancer cells can produce HGF that acts in a paracrine as well as in an autocrine manner. Therefore, HGF and c-Met are putative targets ...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1207063

    authors: Gallego MI,Bierie B,Hennighausen L

    更新日期:2003-11-20 00:00:00

  • Nuclear import of LASP-1 is regulated by phosphorylation and dynamic protein-protein interactions.

    abstract::LASP-1 is a multidomain protein predominantly localized at focal contacts, where it regulates cytoskeleton dynamics and cell migration. However, in different tumor entities, a nuclear LASP-1 accumulation is observed, thought to have an important role in cancer progression. Until now, the molecular mechanisms that cont...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/onc.2012.216

    authors: Mihlan S,Reiß C,Thalheimer P,Herterich S,Gaetzner S,Kremerskothen J,Pavenstädt HJ,Lewandrowski U,Sickmann A,Butt E

    更新日期:2013-04-18 00:00:00

  • Functional inactivation of Cdk9 through oligomerization chain reaction.

    abstract::The oligomerization chain reaction (OCR) strategy is a recently described technique for inactivation of target proteins that function as homoassociate complexes. This novel strategy is based on the fusion of self-associating coiled-coil (CC) domain of the nuclear factor promyelocytic leukemia (PML) to target proteins....

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1206785

    authors: Napolitano G,Mazzocco A,Fraldi A,Majello B,Lania L

    更新日期:2003-07-31 00:00:00

  • Identification of genes associated with tumorigenesis and metastatic potential of hypopharyngeal cancer by microarray analysis.

    abstract::Head and neck squamous cell carcinoma (HNSCC) is the sixth most common cancer among men in the developed world. There is a need, for both clinical and scientific reasons, to find markers to identify patients with aggressive disease as early as possible, and to understand the events leading to malignant transformation ...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1207345

    authors: Cromer A,Carles A,Millon R,Ganguli G,Chalmel F,Lemaire F,Young J,Dembélé D,Thibault C,Muller D,Poch O,Abecassis J,Wasylyk B

    更新日期:2004-04-01 00:00:00

  • BRCA1 interacts with acetyl-CoA carboxylase through its tandem of BRCT domains.

    abstract::Germ-line alterations in BRCA1 are associated with an increased susceptibility to breast and ovarian cancer. BRCA1 is a 220-kDa protein that contains a tandem of two BRCA1 C-Terminal (BRCT) domains. Among missense and nonsense BRCA1 mutations responsible for family breast cancer, some are located into the BRCT tandem ...

    journal_title:Oncogene

    pub_type: 杂志文章

    doi:10.1038/sj.onc.1205915

    authors: Magnard C,Bachelier R,Vincent A,Jaquinod M,Kieffer S,Lenoir GM,Venezia ND

    更新日期:2002-10-03 00:00:00