Rotavirus detection by dot blot hybridization assay using a non-radioactive synthetic oligodeoxynucleotide probe.

Abstract:

:A synthetic oligodeoxynucleotide of 40 nucleotides corresponding to nucleotides 33-72 of the gene coding for the viral protein VP7 of rotavirus, was used as a nucleic acid probe to develop a non-radioactive hybridization method for rotavirus detection. The probe was labelled at the 3' end with biotin-7-dATP. The sensitivity and specificity of the dot blot hybridization assay for rotavirus detection was evaluated with 303 stool specimens. The results indicate that the hybridization assay has a higher sensitivity than both PAGE and EIA. Among the rotavirus strains tested 37 different electropherotypes were found. The results suggest that rotavirus diagnosis by dot hybridization using a non-radioactive probe may become routine laboratory procedure because it is simple, highly specific and very sensitive.

journal_name

Epidemiol Infect

authors

Fernández J,Sandino A,Yudelevich A,Avendaño LF,Venegas A,Hinrichsen V,Spencer E

doi

10.1017/s0950268800049621

subject

Has Abstract

pub_date

1992-02-01 00:00:00

pages

175-84

issue

1

eissn

0950-2688

issn

1469-4409

journal_volume

108

pub_type

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