Abstract:
:A synthetic oligodeoxynucleotide of 40 nucleotides corresponding to nucleotides 33-72 of the gene coding for the viral protein VP7 of rotavirus, was used as a nucleic acid probe to develop a non-radioactive hybridization method for rotavirus detection. The probe was labelled at the 3' end with biotin-7-dATP. The sensitivity and specificity of the dot blot hybridization assay for rotavirus detection was evaluated with 303 stool specimens. The results indicate that the hybridization assay has a higher sensitivity than both PAGE and EIA. Among the rotavirus strains tested 37 different electropherotypes were found. The results suggest that rotavirus diagnosis by dot hybridization using a non-radioactive probe may become routine laboratory procedure because it is simple, highly specific and very sensitive.
journal_name
Epidemiol Infectjournal_title
Epidemiology and infectionauthors
Fernández J,Sandino A,Yudelevich A,Avendaño LF,Venegas A,Hinrichsen V,Spencer Edoi
10.1017/s0950268800049621subject
Has Abstractpub_date
1992-02-01 00:00:00pages
175-84issue
1eissn
0950-2688issn
1469-4409journal_volume
108pub_type
杂志文章abstract::Changes in Helicobacter pylori seroprevalence were studied determining IgG and IgA antibodies of 408 randomly selected adults aged 15-74 years living in Vammala, Finland in 1973 and of 504 similarly selected subjects in 1994. Seroprevalence increased by age at both time points. The age-adjusted seroprevalence rate was...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/s0950268897007565
更新日期:1997-08-01 00:00:00
abstract::The isolation of Fusobacterium necrophorum present in small numbers in heavily contaminated material such as faeces or soil is hampered by the lack of an efficient selective medium and by the high minimum infective dose of the organism. A sensitive method for the detection and isolation of faecal strains of F. necroph...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/s0950268800048469
更新日期:1991-04-01 00:00:00
abstract::We investigated risk factors for severe acute lower respiratory infections (ALRI) among hospitalised children 8 months were at greater risk from influenza-associated ICU admissions and long hospital stay. Children with ADV had increased LOS across all ages. In the first 2 years of life, the effects of different viruse...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268818002017
更新日期:2018-10-01 00:00:00
abstract::We sought to systematically review the published literature describing the epidemiological aspects of the first wave of pandemic A(H1N1) 2009 influenza in the Southern Hemisphere. Fifteen studies were included in this review, originating from South America, Australia or New Zealand, and Africa. Across the different st...
journal_title:Epidemiology and infection
pub_type: 杂志文章,评审
doi:10.1017/S0950268810002037
更新日期:2011-01-01 00:00:00
abstract::Roughly half (54%) of the 910 young conscripts at a garrison in Finland were vaccinated with commercial influenza vaccines in autumn 1998. During the influenza outbreak in February 1999, 12 H3N2-subtype virus strains were isolated from vaccinated patients, and 11 such strains were isolated from unvaccinated patients. ...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/s0950268802007410
更新日期:2002-10-01 00:00:00
abstract::We describe microbiological, clinical and epidemiological aspects of a diphtheria outbreak that occurred in Maranhão, Brazil. The majority of the 27 confirmed cases occurred in partially (n = 16) or completely (n = 10) immunized children (n = 26). Clinical signs and characteristic symptoms of diphtheria such as cervic...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268814001241
更新日期:2015-03-01 00:00:00
abstract::Persistent infection with one or more highly oncogenic human papillomaviruses (HPVs) or high-risk-HPV (HR-HPV) is necessary but not a sufficient aetiological agent for the development of cervical neoplasia. A number of viral, host, environmental and behavioural factors are suggested to be associated with the progressi...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268818001668
更新日期:2018-10-01 00:00:00
abstract::Invasive group A streptococcal (GAS) disease is a condition of clinical and public health significance. We conducted epidemiological analyses to determine if the presence of gastrointestinal (GI) complaints (diarrhea and/or vomiting) early in the course of invasive GAS disease is associated with either of two severe o...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268809990938
更新日期:2010-04-01 00:00:00
abstract::Public Health England conducts enhanced national surveillance of tetanus, a potentially life-threatening vaccine-preventable disease. A standardized questionnaire was used to ascertain clinical and demographic details of individuals reported with clinically suspected tetanus. The 96 cases identified between 2001 and 2...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S095026881600128X
更新日期:2016-12-01 00:00:00
abstract::Three sources of data (general practice episode data from the Weekly Returns Service of the Royal College of General Practitioners, national hospital admission data for England and national mortality data by date of death) were examined separately in each winter (1994/1995 to 1999/2000) to investigate the impact of in...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/s0950268804003231
更新日期:2005-04-01 00:00:00
abstract::The purpose of this research was to determine whether trappers in northern Alaska acquired immunity to rabies virus from non-bite exposures while trapping and skinning arctic foxes (Alopex lagopus). In coastal Alaska recurring epizootics presumably provide trappers ample opportunity for contact with rabid animals. Ser...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/s0950268800051554
更新日期:1994-08-01 00:00:00
abstract::We investigated two sequential outbreaks of severe diarrhoea in two neighbouring villages of Orissa, in 2005. We conducted descriptive and matched case-control studies. The attack rates were 5.6% (n=62) and 5.2% (n=51), respectively, in the first and second villages. One death was reported in the second village (case ...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268808001611
更新日期:2009-06-01 00:00:00
abstract::Current methods of control recruitment for case-control studies can be slow (a particular issue for outbreak investigations), resource-intensive and subject to a range of biases. Commercial market panels are a potential source of rapidly recruited controls. Our study evaluated food exposure data from these panel contr...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268819000219
更新日期:2019-01-01 00:00:00
abstract::To determine if a prediction of epidemic cholera using climate data can be made, we performed autoregression analysis using the data recorded in Dhaka City, Bangladesh over a 20-year period (1983-2002) comparing the number of children aged <10 years who were infected with Vibrio cholerae O1 to the maximum and minimum ...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268807008175
更新日期:2008-01-01 00:00:00
abstract::Eradication of poliomyelitis most likely will occur. In fact, it is almost gone from the Western Hemisphere. Health workers in Sweden, Finland, and the Netherlands routinely vaccinate almost all children with the inactivated poliovaccines (IPV). Despite good vaccination coverage, polio can still occur. For example...
journal_title:Epidemiology and infection
pub_type: 杂志文章,评审
doi:10.1017/s0950268800049451
更新日期:1992-02-01 00:00:00
abstract::The objective of this study was to model the age-time-dependent incidence of hepatitis B while estimating the impact of vaccination. While stochastic models/time-series have been used before to model hepatitis B cases in the absence of knowledge on the number of susceptibles, this paper proposed using a method that fi...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268807008692
更新日期:2008-03-01 00:00:00
abstract::Two epidemics of acute, watery diarrhoea in villages in North Arcot district, India, were investigated. The attack rates were 10.03 and 15.53 per 100 population, the median duration was 5 days and enteric pathogens were present in 56.8% and 60.3% of specimens from the two villages, but no predominant pathogen was iden...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/s0950268801005799
更新日期:2001-08-01 00:00:00
abstract::Antimicrobial use in pig farming is influenced by a range of risk factors, including herd characteristics, biosecurity level, farm performance, occurrence of clinical signs and vaccination scheme, as well as farmers' attitudes and habits towards antimicrobial use. So far, the effect of these risk factors has been expl...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268818000742
更新日期:2018-06-01 00:00:00
abstract::During the 10-year period 1978-87 there were 48 outbreaks of food poisoning in Scottish hospitals affecting a total of 2287 persons of whom 12 died. This compared with 50 outbreaks during the previous 5 years (1973-77) when over 1500 persons and 7 deaths were recorded. Although the incidence of outbreaks has decreased...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/s095026880002954x
更新日期:1988-12-01 00:00:00
abstract::During a 3-year period Branhamella catarrhalis was isolated in significant numbers from 239 (1.3%) of 19,488 specimens of sputum sent for routine microbiological examination at a 700-bed general hospital. The majority of patients (83%) were over 60 years of age and 65% were male. There was a distinct seasonal variatio...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/s0950268800067947
更新日期:1987-10-01 00:00:00
abstract::Capture-recapture analysis has been used to evaluate infectious disease surveillance. Violation of the underlying assumptions can jeopardize the validity of the capture-recapture estimates and a tool is needed for cross-validation. We re-examined 19 datasets of log-linear model capture-recapture studies on infectious ...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268807008254
更新日期:2008-01-01 00:00:00
abstract::Multi-locus sequence typing was performed on 1003 Campylobacter jejuni isolates collected in a 2-year longitudinal study of 15 dairy farms and four sheep farms in Lancashire, UK. There was considerable farm-level variation in occurrence and prevalence of clonal complexes (CC). Clonal complexes ST61, ST21, ST403 and ST...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268810002736
更新日期:2011-11-01 00:00:00
abstract::The one-point MCA method is very simple to perform and useful as a screening test in diagnosing leptospirosis in routine clinical laboratories. The kit, sensitized with six serovars occurring in Japan, was also useful in detecting serum antibodies of patients with leptospirosis in China. ...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/s095026880006787x
更新日期:1987-10-01 00:00:00
abstract::Orf of the hand is an uncommon zoonotic infection caused by a dermotropic DNA virus that belongs to the Parapoxvirus genus of the family Poxviridae. It is transmitted to humans through contact with infected sheep and goats and is reported as an occupational disease. We report nine cases of human orf seen in the hands ...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/s0950268805003778
更新日期:2005-08-01 00:00:00
abstract::Effective primary prevention of congenital toxoplasmosis requires up to date information on locally relevant risk factors for infection in pregnant women. In Naples, risk factors for toxoplasma infection were compared in recently infected women (as assessed by detection of specific IgM in serum) and susceptible, IgG n...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/s0950268800052675
更新日期:1996-06-01 00:00:00
abstract::Streptococcus pyogenes (or Group A Streptococcus, GAS) is a Gram-positive human pathogen responsible for a diverse array of superficial, invasive and immune-related diseases. GAS infections have historically been diseases of poverty and overcrowding, and remain a significant problem in the developing world and in disa...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268818002285
更新日期:2018-08-15 00:00:00
abstract::As influenza vaccination is now widely recommended, randomized clinical trials are no longer ethical in many populations. Therefore, observational studies on patients seeking medical care for acute respiratory illnesses (ARIs) are a popular option for estimating influenza vaccine effectiveness (VE). We developed a pro...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268814002179
更新日期:2015-05-01 00:00:00
abstract::Transmission of bovine herpesvirus 1 (BHV1) within and between herds was studied on the island of Ameland, The Netherlands. There were 50 herds with 3300 head of cattle on the island. Herds were divided into three groups: (1) only containing seronegative cattle, (2) containing seronegative cattle and vaccinated seropo...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:
更新日期:2003-06-01 00:00:00
abstract::Horizontal transmission of Campylobacter jejuni was investigated in campylobacter-free broiler chicks. One hundred and twenty chicks housed individually, were provided with water containing 10(2)-10(9) c.f.u./ml C. jejuni. Colonization was rapid [47 of 73 (64%) positive cloacal cultures within 3 days and 65 of 73 (89%...
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/s0950268800054571
更新日期:1990-02-01 00:00:00
abstract::Although community-onset bloodstream infection (BSI) is recognized to be a major cause of morbidity and mortality, there is a paucity of population-based studies defining its overall burden. We conducted population-based laboratory surveillance for all community-onset BSI in the Calgary Health Region during 2000-2004....
journal_title:Epidemiology and infection
pub_type: 杂志文章
doi:10.1017/S0950268806007631
更新日期:2007-08-01 00:00:00