Defensive role of pleural mesothelial cell sialomucins in tumor metastasis.

Abstract:

STUDY OBJECTIVES:Sialomucin complex (SMC) is a heterodimeric glycoprotein, and is found on the surfaces of the mesothelia of the pleura, pericardium, and peritoneum. Sialomucins play a significant role in adhesion as well as in defense. In this study, we hypothesized that pleural mesothelial cells (PMCs) express SMC and thus prevent the adherence of ovarian cancer cells (HTB-77) to the pleura. METHODS:PMCs were plated, and the adherence of HTB-77 cells was observed using a cytofluor. The PMC monolayer was pretreated with sialidase, and HTB-77 adherence was observed using cytofluor. In another set of HTB-77 cells, adherence was observed when the PMC monolayer was pretreated with supernatants of HTB-77 cells. Last, supernatants of HTB-77 cells were assayed for sialidase activity. RESULTS:The removal of SMC by sialidase greatly increased the adherence of HTB-77 cells to the PMC monolayer, which was statistically significant (p < 0.05). Similar results were obtained when the PMC monolayer was pretreated with the supernatants of HTB-77 cells. Supernatants of HTB-77 cells showed the presence of sialidase. CONCLUSIONS:The presence of SMC on the PMC acts as a defense layer, and its removal by sialidase increases the susceptibility of the PMC layer to the adherence of malignant cells and to increased metastasis. HTB-77 cells also express sialidase, which by its action on the monolayer aids in the adherence of tumor cells to the pleural surface.

journal_name

Chest

journal_title

Chest

authors

Sharma RK,Mohammed KA,Nasreen N,Hardwick J,Van Horn RD,Ramirez-Icaza C,Antony VB

subject

Has Abstract

pub_date

2003-08-01 00:00:00

pages

682-7

issue

2

eissn

0012-3692

issn

1931-3543

pii

S0012-3692(15)33356-0

journal_volume

124

pub_type

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