Apoptosis of CD34+ cells after incubation with sera of leukopenic patients with systemic lupus erythematosus.

Abstract:

:Leukopenia and anaemia are observed in about a fifth of all patients with systemic lupus erythematosus (SLE) and may be due either to the destruction of blood cells or their decreased production. The former may be humoral or cell-mediated or result from apoptosis of peripheral blood cells. Several observations suggest the occurrence of the latter reduced in vitro proliferation of pluripotent bone marrow progenitors from the bone marrow aspirates of SLE patients,reduced counts of CD34+ cells in bone marrow aspirates in SLE patients, apoptosis of lymphopoietic progenitors and apoptosis of bone marrow cells. The aim of our study was to investigate whether humoral factors may induce suppression of haematopoiesis by increased apoptosis of CD34+ cells. For this purpose, we incubated allogeneic CD34+-enriched cells with sera of 18 leukopenic SLE patients. Apoptosis was induced by four of 18 sera. This effect was independent of complement-inhibition and FAS-blockade. Although reduced proliferation of autologous pluripotent bone marrow progenitors has been attributed to an IgG serum inhibitor, removal of IgG from these four proapoptotic sera had no effect on apoptosis of allogeneic CD34+ cells. The proapoptotic effect was associated with high titres of anti-dsDNA antibodies and low haemoglobin concentrations, but not with high titres of antinuclear antibodies, TNF-alpha and IFN-alpha of the sera tested.

journal_name

Lupus

journal_title

Lupus

authors

Tiefenthaler M,Bacher N,Linert H,Mühlmann O,Hofer S,Sepp N,Amberger A,Geisen F,Obermoser G,Konwalinka G

doi

10.1191/0961203303lu423oa

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

471-8

issue

6

eissn

0961-2033

issn

1477-0962

journal_volume

12

pub_type

杂志文章

相关文献

LUPUS文献大全
  • Protein S deficiency revealed by skin necrosis in a patient with lupus.

    abstract::Protein S deficiency is rare in systemic lupus erythematosus (SLE) and is generally associated with the presence of antiphospholipid (APL) antibodies. Lack of protein S can cause skin necrosis, but when it does it is generally in response to warfarin exposure. In this article, we describe the case of a patient who had...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203319843345

    authors: Plana-Pla A,Bielsa Marsol I,Ferrandiz Foraster C

    更新日期:2019-06-01 00:00:00

  • Primary antiphospholipid antibody syndrome presenting with adrenal insufficiency in a child: case report and review of literature.

    abstract::A 7-year-old boy presented with adrenal insufficiency. He subsequently developed venous thrombosis in the limbs and was diagnosed with primary antiphospholipid syndrome (PAPS) based on clinical and laboratory parameters. Both adrenals were normal on imaging. He required thrombolysis and anticoagulation. The progressiv...

    journal_title:Lupus

    pub_type: 杂志文章,评审

    doi:10.1177/0961203310397965

    authors: Bhakhri BK,Katewa S,Sharma R,Mahajan S

    更新日期:2011-10-01 00:00:00

  • Clinical significance of phospholipid-dependent anti-beta 2-glycoprotein I (beta 2-GPI) antibodies in systemic lupus erythematosus.

    abstract::We investigated the clinical significance of IgG phospholipid-dependent anti-beta 2-glycoprotein I (beta 2-GPI) antibodies in patients with SLE. The study population consisted of 140 patients with SLE. Sera were examined for IgG phospholipid-dependent anti-beta 2-GPI antibodies by ELISA. IgG phospholipid-dependent ant...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/096120339500400609

    authors: Kaburaki J,Kuwana M,Yamamoto M,Kawai S,Matsuura E,Ikeda Y

    更新日期:1995-12-01 00:00:00

  • Naturally occurring anti-idiotypic antibodies reactive with anti-DNA antibodies in systemic lupus erythematosus.

    abstract::The levels of 'putative' anti-idiotypic antibodies reactive with F(ab')2 fragments of affinity purified DNA binding antibodies from five SLE patients were measured in the serum of active and inactive systemic lupus erythematosus (SLE) patients, their relatives, spouses and healthy non-related individuals. Serum anti-i...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1191/096120398678919958

    authors: Williams WM,Isenberg DA

    更新日期:1998-01-01 00:00:00

  • Vitamin D and cutaneous lupus erythematosus: effect of vitamin D replacement on disease severity.

    abstract:BACKGROUND:The main vitamin D source is exposure to ultraviolet radiation, which aggravates cutaneous lupus erythematosus (CLE). OBJECTIVES:The aims of this study were to identify variables associated with lower serum 25-hydroxyvitamin D [25(OH)D] levels in CLE patients and assess the effect of vitamin D restoration o...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203314522338

    authors: Cutillas-Marco E,Marquina-Vila A,Grant WB,Vilata-Corell JJ,Morales-Suárez-Varela MM

    更新日期:2014-06-01 00:00:00

  • Primary anetoderma: a cutaneous sign of antiphospholipid antibodies.

    abstract::Although a few reports in recent years have suggested that patients with antiphospholipid antibodies (aPL) are prone to developing primary anetoderma (PA), it is still unclear how often aPL are detected in unselected PA patients. We studied nine consecutive PA patients for the presence of autoimmune antibodies and dis...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1191/0961203303lu403oa

    authors: Hodak E,Feuerman H,Molad Y,Monselise Y,David M

    更新日期:2003-01-01 00:00:00

  • SLEDAI-2K Responder Index 50 captures 50% improvement in disease activity over 10 years.

    abstract:OBJECTIVES:To determine the frequency and the time to complete recovery identified by Systemic Lupus Erythematosus Disease Activity Index 2000 (SLEDAI-2K) and the time to partial recovery identified by the SLEDAI-2K Responder Index 50 (SRI-50) in three laboratory systems over 10 years. METHODS:This is a retrospective ...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203312454344

    authors: Touma Z,Gladman DD,Ibañez D,Urowitz MB

    更新日期:2012-10-01 00:00:00

  • Vitamin D insufficiency in a large female SLE cohort.

    abstract::The objective of this study was to determine the vitamin D status and its relationship with disease and therapy features and with bone mineral density in women with systemic lupus erythematosus. Non-pregnant systemic lupus erythematosus women with dual-energy X-ray absorptiometry and vitamin D measurements performed b...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203309345775

    authors: Toloza SM,Cole DE,Gladman DD,Ibañez D,Urowitz MB

    更新日期:2010-01-01 00:00:00

  • Sulpha allergy in lupus patients: a clinical perspective.

    abstract::Systemic lupus erythematosus is a chronic, relapsing autoimmune disease that can affect multiple organ systems. An increased prevalence of drug allergy has been reported in lupus patients compared with the general population. Using a cohort of 417 lupus patients, we found a history of sulpha allergy in 27.3% of patien...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203307086640

    authors: Jeffries M,Bruner G,Glenn S,Sadanandan P,Carson CW,Harley JB,Sawalha AH

    更新日期:2008-03-01 00:00:00

  • Twenty-year follow-up: an unusual case of nephropathy of antiphospholipid syndrome.

    abstract::Nephropathy of antiphospholipid antibody syndrome (NAPS) is an increasingly well-recognized aspect of antiphospholipid syndrome. The most characteristic histopathology is that of thrombotic microangiopathy, and thrombosis occurring in the renal vasculature is thought to be the initiating event. Other less common patho...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203308098629

    authors: Lin YQ,Lakshminarayanan S,Yamase H,Palmisano J,Parke A

    更新日期:2009-05-01 00:00:00

  • Diagnostic ANCA algorithms in daily clinical practice: evidence, experience, and effectiveness.

    abstract::Detection of antineutrophil cytoplasmic antibodies (ANCA) for ANCA-associated vasculitides (AAV) is based on indirect immunofluorescence (IIF) on ethanol-fixed neutrophils and reactivity toward myeloperoxidase (MPO) and proteinase 3 (PR3). According to the international consensus for ANCA testing, presence of ANCA sho...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203316640921

    authors: Avery TY,Bons J,van Paassen P,Damoiseaux J

    更新日期:2016-07-01 00:00:00

  • Affinity purified human antiphospholipid antibodies bind normal term placenta.

    abstract::Antiphospholipid antibodies (aPL) are associated with an increased incidence of fetal loss, but the pathophysiology remains unclear. One mechanism may involve the binding of aPL directly to the placenta where they may initiate placental thrombosis and infarction. We have developed an immunofluorescent technique to det...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1191/096120399678840756

    authors: Donohoe S,Kingdom JC,Mackie IJ

    更新日期:1999-01-01 00:00:00

  • Repeated miscarriages in patients with antiphospholipid syndrome and subjected to in vitro fertilization: the importance of preimplantation genetic diagnosis.

    abstract::The aim of this study was to investigate two patients with antiphospholipid syndrome (APS) who suffered from multiple repeated pregnancy losses of probably genetically impaired embryos. Sera from these patients contained high levels of IgG antibodies against cardiolipin, IgG and IgM phosphatidyl inositol, IgG phosphat...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203312442581

    authors: Ulcova-Gallova Z

    更新日期:2012-06-01 00:00:00

  • Urinary biomarkers for lupus nephritis: the role of the vascular cell adhesion molecule-1.

    abstract::Renal involvement is one of the main complications of systemic lupus erythematosus, causing a significant impact on patients' morbidity and mortality. Renal biopsy is still the gold standard of diagnosis, but it has many limitations. In this sense, several recent studies aim to identify biomarkers that not only predic...

    journal_title:Lupus

    pub_type: 杂志文章,评审

    doi:10.1177/0961203319826695

    authors: Gasparin AA,Pamplona Bueno de Andrade N,Hax V,Tres GL,Veronese FV,Monticielo OA

    更新日期:2019-03-01 00:00:00

  • European registry of babies born to mothers with antiphospholipid syndrome: a result update.

    abstract::The registry is a prospective, European, multicentric, longitudinal study, which follows a cohort of children born to mothers with antiphospholipid syndrome (APS). It was started in 2003. In this report, we update the results obtained from the study of 110 mothers and 112 children (two twin births). Eighty per cent of...

    journal_title:Lupus

    pub_type: 杂志文章,多中心研究

    doi:10.1177/0961203309106951

    authors: Boffa MC,Lachassinne E,Boinot C,De Carolis S,Rovere-Querini P,Avcin T,Biasini-Rebaioli C,Le Toumelin P,Aurousseau MH,Tincani A

    更新日期:2009-09-01 00:00:00

  • Anti-glycan antibodies as biomarkers for diagnosis and prognosis.

    abstract::Glycans (sugars or carbohydrates) are predominant surface components of cells such as erythrocytes, immune cells and microorganisms. As such, they give rise to high levels of anti-glycan antibodies of all classes. Antibodies to certain defined mono, di and oligosaccharides that are common in bacterial, fungal and para...

    journal_title:Lupus

    pub_type: 杂志文章,评审

    doi:10.1191/0961203306lu2331oa

    authors: Dotan N,Altstock RT,Schwarz M,Dukler A

    更新日期:2006-01-01 00:00:00

  • Biologics targeting type I interferons in SLE: A meta-analysis and systematic review of randomised controlled trials.

    abstract:OBJECTIVE:The feed-forward loop of type I interferons (IFNs) production and subsequent immunopathology of systemic lupus erythematosus (SLE) has been hypothesised to be disrupted with inhibition of IFNα or type I IFN receptor subunit 1 (IFNAR). This systematic review and meta-analysis present the treatment efficacy and...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203320959702

    authors: Koh JWH,Ng CH,Tay SH

    更新日期:2020-12-01 00:00:00

  • Recurrent urinary tract infections and bladder dysfunction in systemic lupus erythematosus.

    abstract::To assess bladder function in systemic lupus erythematosus (SLE) patients with recurrent urinary tract infections (UTIs). A convenience sample of consecutive patients with SLE (American College of Rheumatology criteria), with recurrent UTIs (>/=3 events in the preceding 12 months), without history of central nervous s...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203308093458

    authors: Duran-Barragan S,Ruvalcaba-Naranjo H,Rodriguez-Gutierrez L,Solano-Moreno H,Hernandez-Rios G,Sanchez-Ortiz A,Ramos-Remus C

    更新日期:2008-12-01 00:00:00

  • Disease activity, cumulative damage and quality of life in systematic lupus erythematosus: results of a cross-sectional study.

    abstract::The relationship between disease activity, cumulative damage and self-reported quality of life was examined in 96 patients with Systematic Lupus Erythematosus (SLE). Disease activity was measured by the SLE Disease Activity Index (SLEDAI) and cumulative damage by the Systematic Lupus International Cooperating Clinics/...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/096120339700600305

    authors: Hanly JG

    更新日期:1997-01-01 00:00:00

  • Infant perinatal thrombosis and antiphospholipid antibodies: a review.

    abstract::Perinatal thrombosis in infants born to mothers with antiphospholipid antibodies (aPL) is a rare event, but with risk of death or severe sequelae. We analysed 16 infants with such perinatal thrombosis reported in the literature in the last 20 years. Thromboses were arterial (13/16), mostly strokes (8/16). Hydrops feta...

    journal_title:Lupus

    pub_type: 杂志文章,评审

    doi:10.1177/0961203307079039

    authors: Boffa MC,Lachassinne E

    更新日期:2007-01-01 00:00:00

  • Sensitivity and specificity of autoantibody tests in the differential diagnosis of lupus nephritis.

    abstract::Several studies have investigated the potential of various autoantibody tests in the diagnosis of systemic lupus erythematosus (SLE). Many lupus patients initially present with glomerulonephritis. In that clinical situation the main differential diagnosis are other forms of glomerulonephritis. In this study the diagno...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203309345753

    authors: Heidenreich U,Mayer G,Herold M,Klotz W,Stempfl Al-Jazrawi K,Lhotta K

    更新日期:2009-12-01 00:00:00

  • Genetically determined mannan-binding lectin deficiency is of minor importance in determining susceptibility to severe infections and vascular organ damage in systemic lupus erythematosus.

    abstract::Deficiency of mannan-binding lectin (MBL) has been reported to impact susceptibility to severe infections and atherosclerosis in systemic lupus erythematosus (SLE). In this study, MBL gene polymorphisms were analysed in 143 SLE patients and the frequency of severe infections and organ damage according to SLICC/ACR Dam...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/09612033070160040201

    authors: Jönsen A,Gullstrand B,Güner N,Bengtsson AA,Nived O,Truedsson L,Sturfelt G

    更新日期:2007-01-01 00:00:00

  • Autoantibodies in systemic lupus erythematosus: comparison of historical and current assessment of seropositivity.

    abstract::Systemic lupus erythematosus (SLE) is characterized by multiple autoantibodies and complement activation. Recent studies have suggested that anti-nuclear antibody (ANA) positivity may disappear over time in some SLE patients. Anti-double-stranded DNA (dsDNA) antibody titers and complement levels may vary with time and...

    journal_title:Lupus

    pub_type: 历史文章,杂志文章

    doi:10.1177/0961203310385738

    authors: Ippolito A,Wallace DJ,Gladman D,Fortin PR,Urowitz M,Werth V,Costner M,Gordon C,Alarcón GS,Ramsey-Goldman R,Maddison P,Clarke A,Bernatsky S,Manzi S,Bae SC,Merrill JT,Ginzler E,Hanly JG,Nived O,Sturfelt G,Sanchez-Gu

    更新日期:2011-03-01 00:00:00

  • Standardization of lupus anticoagulant. The Lupus Anticoagulant Sensitivity Index (LASI).

    abstract::Results for lupus anticoagulant (LA) are expressed as ratio of patient-to-normal clotting times (LA-ratio) according to the equation LA-ratio = (Patient(Clotting time)/Normal(Clotting time)). However, numerical results vary according to the method used for testing, thus making difficult the between-method and between-...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203312438114

    authors: Tripodi A,Chantarangkul V,Pengo V

    更新日期:2012-06-01 00:00:00

  • False-positive IgM for CMV in pregnant women with autoimmune disease: a novel prognostic factor for poor pregnancy outcome.

    abstract::Our aims were to assess the frequency of false-positive IgM antibodies for cytomegalovirus in pregnant women with autoimmune diseases and in healthy women (controls) and to determine their relationship with pregnancy outcome. Data from 133 pregnancies in 118 patients with autoimmune diseases and from 222 pregnancies i...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203310361350

    authors: De Carolis S,Santucci S,Botta A,Garofalo S,Martino C,Perrelli A,Salvi S,Degennaro V,de Belvis A,Ferrazzani S,Scambia G

    更新日期:2010-06-01 00:00:00

  • Objectively measured physical activity and its influence on physical capacity and clinical parameters in patients with primary Sjögren's syndrome.

    abstract::Objective The objectives of this paper are to objectively measure habitual physical activity levels in patients with primary Sjögren's syndrome (pSS) with mild disease activity and to determine to which extent it may be associated with physical capacity and function and clinical features. Methods In this cross-section...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/0961203316674819

    authors: Dassouki T,Benatti FB,Pinto AJ,Roschel H,Lima FR,Augusto K,Pasoto S,Pereira RMR,Gualano B,de Sá Pinto AL

    更新日期:2017-06-01 00:00:00

  • Autoimmune thyroid disorders in SLE in Singapore.

    abstract::One hundred and twenty-nine patients with systemic lupus erythematosus were screened for thyroid function tests. Of these, 8.9% had hyperthyroidism and 3.9% Hashimoto's thyroiditis. Euthyroid sick syndrome was present in 47.8% of patients. There was a high prevalence of antimicrosomal and antithyroglobulin antibodies ...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/096120339300200109

    authors: Boey ML,Fong PH,Lee JS,Ng WY,Thai AC

    更新日期:1993-02-01 00:00:00

  • Association between shortened telomere length and systemic lupus erythematosus: a meta-analysis.

    abstract::Objective We aimed to evaluate the relationship between telomere length and systemic lupus erythematosus (SLE). Methods PUBMED and EMBASE databases were searched; meta-analyses were performed comparing telomere length in SLE patients and healthy controls, and on SLE patients in subgroups based on ethnicity, sample typ...

    journal_title:Lupus

    pub_type: 杂志文章,meta分析

    doi:10.1177/0961203316662721

    authors: Lee YH,Jung JH,Seo YH,Kim JH,Choi SJ,Ji JD,Song GG

    更新日期:2017-03-01 00:00:00

  • Microchimerism and human autoimmune diseases.

    abstract::Cells traffic in both directions between the fetus and the mother during pregnancy. Recent studies indicate that a low level of fetal cells commonly persists in the maternal circulation for years after pregnancy completion. The harboring of DNA or cells from another individual at low levels is called microchimerism. C...

    journal_title:Lupus

    pub_type: 杂志文章,评审

    doi:10.1191/0961203302lu271oa

    authors: Nelson JL

    更新日期:2002-01-01 00:00:00

  • Antiphospholipid syndrome and the idiotypic network.

    abstract::To study whether monoclonal anticardiolipin antibodies (aCL), derived from patients with antiphospholipid syndrome (APS), have similar pathogenic potential, we have employed an experimental model of antiphospholipid syndrome. Monoclonal aCL were produced by the combined method of EBV transformation and somatic cell hy...

    journal_title:Lupus

    pub_type: 杂志文章

    doi:10.1177/096120339500400307

    authors: Bakimer R,Blank M,Kosashvilli D,Ichikawa K,Khamashta MA,Hughes GR,Koike T,Shoenfeld Y

    更新日期:1995-06-01 00:00:00