Abstract:
:The ENOD40 gene, found in several leguminous and non-leguminous plant species is expressed in the pericycle of legume roots early in the nodulation process, adjacent to the protoxylem poles, but before the cortical cells divide to form the nodule itself. The ENOD40 transcript contains only short open reading frames which give rise to short peptides with a signaling function. The current work reports the cloning and analysis of ENOD40 genes from perennial ryegrass (Lolium perenne) and barley (Hordeum vulgare). Specific polymerase chain reaction (PCR) techniques lead to the isolation of a 659 bp cDNA encoding an ENOD40 homologue, designated LpE-NOD40, from a Lolium perenne stem cDNA library. In addition, a partial ENOD40 cDNA of 384 bp was isolated from barley (Hordeum vulgare) by RT-PCR cloning. The LpENOD40 transcript encodes a putative dodecapeptide, similar to that identified in ENOD40s from leguminous plants and other dicots, and also to ENOD40s from monocots. The coding sequences of ryegrass and barley ENOD40 are represented by a short open reading frame of 12 amino acids. These show a high degree of similarity to each other and to other ENOD40 sequences from monocots. The corresponding genomic DNA from a genomic ryegrass lambda library revealed that the LpENOD40 gene contains no introns. Southern blot analysis shows that the ryegrass genome contains a single copy, possibly two copies, of the gene. Alignment of the ENOD40 cDNA sequences from ryegrass and barley revealed high (77%) nucleotide homology. The ENOD40 peptides are highly conserved, not only among monocots but also on comparison with the dicot peptides. The amino acid identity of region I from ryegrass with its counterparts in maize, barley and rice is 92, 83 and 75% respectively. Expression analysis by RT-PCR demonstrates that a high level of LpENOD40 gene transcript was found expressed in stem tissue, while a lower level was detected in leaves and only a very low expression in flowers of perennial ryegrass. No LpENOD40 transcript was detected in roots.
journal_name
J Plant Physioljournal_title
Journal of plant physiologyauthors
Larsen Kdoi
10.1078/0176-1617-00962subject
Has Abstractpub_date
2003-06-01 00:00:00pages
675-87issue
6eissn
0176-1617issn
1618-1328pii
S0176-1617(04)70452-6journal_volume
160pub_type
杂志文章abstract::Increasing soil salinity is often associated with accelerated ethylene production in plants, leading to overall growth reduction. The salt-tolerant 1-aminocyclopropane-1-carboxylic acid (ACC) deaminase-producing PGPR may alleviate salt stress by reducing the production of stress ethylene. In this study, a salt-toleran...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2018.10.010
更新日期:2018-12-01 00:00:00
abstract::Four-day-old etiolated cucumber seedlings (Cucumis sativus L.) were transferred to cool-white-fluorescent light (15 mumol m-2 s-1) for 1 h and 24 hours and etiochloroplasts and chloroplasts were isolated from developing cotyledons. Plastids were fractionated to stroma, envelope and thylakoid or inner membranes and the...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1078/0176-1617-00848
更新日期:2003-01-01 00:00:00
abstract::The uptake of liposomal contents into Catharanthus roseus protoplasts has been quantitated. Hi.phest uptake was obtained from positively charged vesicles (1.09% of vesicle contents per 10(7) protoplasts) in the presence of 10% w/v polyethylene glycol 4000. Significant uptake was also observed from negatively charged v...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(84)80014-0
更新日期:1984-11-01 00:00:00
abstract::Needle differentiation is a very complex process associated with the formation of a mature photosynthetic organ. From meristem differentiation to leaf maturation, gene control must play an important role switching required genes on and off to define tissue functions, with the epigenetic code being one of the main regu...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.09.018
更新日期:2010-03-15 00:00:00
abstract::The application of nitrification inhibitors (NIs) together with nitrogen fertilizers in grasslands is an effective alternative to reduce nitrate leaching and nitrogenous gases emissions to the atmosphere. Nevertheless, the use of NIs increases the amount of ammonium available for the plant that, due to its reported to...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2006.11.013
更新日期:2007-12-01 00:00:00
abstract::Plants resistant to triazine-type herbicides are known to be altered in their photosystem II reaction center. Serine at site 264 in D1 protein is replaced by glycine. The measurements of chlorophyll a fluorescence excitations with a variable number of saturating flashes in Chenopodium album plants show characteristic ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2009.04.011
更新日期:2009-10-15 00:00:00
abstract::Drought is one of the stresses that limit the yield of barley. Despite extensive studies focused on the issue, the molecular mechanism of the response to drought is still not fully understood. In our previous study, we proposed drought-induced signal perception controlled by actin filaments (AFs). To test this hypothe...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.02.006
更新日期:2016-04-01 00:00:00
abstract::The effect of subculture interval and total culture age on plant regeneration from serially subcultured, leaf derived embryogenic callus cultures of Pennisetum purpureum (Napier grass) was determined. During 38 weeks of culture both embryogenic callus proliferation and the yield of plants was maximal for callus from f...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(84)80028-0
更新日期:1984-12-01 00:00:00
abstract::Ion-selective microelectrodes are a powerful tool in studying adaptive responses of plant cells and tissues to various abiotic stresses. However, application of this technique in Na(+) flux measurements was limited due to poor selectivity for Na(+) ions of commercially available Na(+) cocktails. Often, these cocktails...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.12.006
更新日期:2011-07-01 00:00:00
abstract::Pokkah boeng disease (PBD) is a foliar disease causing severe losses in sugarcane crop production. Research into resistance mechanisms against the causal agent, Fusarium verticillioides, is particularly important for farmers and researchers. This work based on the comprehensive analysis of metabolic, proteomic, and bi...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2020.153207
更新日期:2020-08-01 00:00:00
abstract::We investigated the effects of short-term salinity stress and spermidine application to salinized nutrient solution on polyamine metabolism and various stress defense reactions in the roots of two cucumber (Cucumis sativus L.) cultivars, Changchun mici and Jinchun No. 2. Seedlings grown in nutrient solution salinized ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2007.11.006
更新日期:2008-10-09 00:00:00
abstract::No significant changes were detected in the proportions of the various monosaccharides present in the cell wall polysaccharides of the peripheral cell layers on the upper and lower sides of sunflower hypocotyls which had undergone gravicurvature. The incorporation of radioactive glucose into the major classes of polys...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(85)80164-4
更新日期:1985-02-01 00:00:00
abstract::Physiological diversification reflects adaptation for specific environmental challenges. As the major physiological process that provides plants with carbon and energy, photosynthesis is under strong evolutionary selection that gives rise to variability in nearly all parts of the photosynthetic apparatus. Here, we dis...
journal_title:Journal of plant physiology
pub_type: 杂志文章,评审
doi:10.1016/j.jplph.2014.07.024
更新日期:2015-01-01 00:00:00
abstract::Measurements of chlorophyll a fluorescence, nutrient and trace elements, total protein content and malonyldialdehyde in leaves of Arabidopsis thaliana between 1 and 192 h after treatment with 0, 1 or 3 mM 2-3H-benzoxazolinone (BOA), together with imaging of chlorophyll a fluorescence and of the distributions of hydrog...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.11.011
更新日期:2011-06-15 00:00:00
abstract::Cadmium (Cd) is a non-essential trace element and its environmental concentrations are approaching toxic levels, especially in some agricultural soils. Understanding how and where Cd is stored in plants is important for ensuring food safety. In this study, we examined two plant species that differ in the distribution ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2012.07.011
更新日期:2012-12-15 00:00:00
abstract::In C4 plants, phosphoenolpyruvate carboxykinase (PEPCK) plays a key role in the C4 cycle. PEPCK is also involved in gluconeogenesis and is conserved in both lower and higher organisms, including in animals and plants. A phylogenic tree constructed from PEPCK sequences from bacteria to higher plants indicates that the ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.02.008
更新日期:2017-06-01 00:00:00
abstract::Plants are able to acclimate to highly fluctuating light environment and evolved a short- and long-term light acclimatory responses, that are dependent on chloroplasts retrograde signalling. In this review we summarise recent evidences suggesting that the chloroplasts act as key sensors of light intensity changes in a...
journal_title:Journal of plant physiology
pub_type: 杂志文章,meta分析,评审
doi:10.1016/j.jplph.2013.06.005
更新日期:2013-11-15 00:00:00
abstract::Ascorbic acid oxidase (AAO) is a plant blue-copper protein catalyzing dioxygen reduction to water using ascorbic acid as the electron donor. In spite of extensive molecular characterization the physiological role of AAO is still uncertain. Abundant mRNA, protein and activity of AAO were observed in illuminated leaves ...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.09.016
更新日期:2007-01-01 00:00:00
abstract::Plant lipid transfer proteins (LTPs) are low-molecular-mass proteins whose biological function still remains elusive. They are synthesized with a signal peptide that drives them to the secretory pathway. We have previously described the occurrence of an apoplastic LTP named Ha-AP10, present in sunflower seeds. Using a...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2008.11.005
更新日期:2009-05-15 00:00:00
abstract::Nodule conductance to O2 diffusion has been involved as a major factor of the inhibition of N2 fixation by soil salinity that severely reduces the production of grain legumes. In order to determine the effect of this constraint on the nodule conductance, oxygen uptake by the nodulated roots of Cicer arietinum was meas...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2006.05.016
更新日期:2007-08-01 00:00:00
abstract::Total phenolics and the profile of phenolic acids and flavonoids were investigated in the roots and leaves of Ulmus laevis cultured on the medium with inorganic and organic arsenic - As(III), As(V) and DMA(V) at 0.06mM and their equimolar combinations. Further, the accumulation of salicylic acid (free and glucoside-bo...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.09.013
更新日期:2018-01-01 00:00:00
abstract::The assimilation and availability of nitrogen in its different forms can significantly affect the response of primary productivity under the current atmospheric alteration and soil degradation. An elevated CO2 concentration (e[CO2]) triggers changes in the efficiency and efficacy of photosynthetic processes, water use...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2016.05.020
更新日期:2016-08-01 00:00:00
abstract::Strigolactones (SLs) or closely related molecules were recently identified as phytohormones, acting as long-distance branching factors that suppress growth of pre-formed axillary buds in the shoot. The SL signaling pathways and light appear to be connected, as SLs were shown to induce light-regulated pathways and to m...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2011.05.022
更新日期:2011-11-01 00:00:00
abstract::Nod factors (Lipo-chitooligosaccharides, or LCOs) act as bacteria-to-plant signal molecules that modulate early events of the Bradyrhizobium-soybean symbiosis. It is known that low root zone temperature inhibits the early stages of this symbiosis; however, the effect of low soil temperature on bacteria-to-plant signal...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2005.04.029
更新日期:2006-01-01 00:00:00
abstract::After a basic fluorescent and incandescent illumination, a period of incandescent illumination was interrupted by 3 hours of darkness at different times, the total light period always being 16 h. After these Day Interruption (DI) treatments of various durations the plants were transferred to long day (LD) or continuou...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/S0176-1617(84)80008-5
更新日期:1984-12-01 00:00:00
abstract::Sorghum is a C4 plant adapted to semi-arid environments, and characterized by high water-use efficiency. To better understand the molecular and physiological basis of drought response the sorghum genotype IS19453, selected as a drought tolerant line during field trials, was evaluated in a "dry-down" experiment under c...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2013.12.014
更新日期:2014-04-15 00:00:00
abstract::Both H(2)O(2) and NO are involved in multiple physiological responses in plants. Metallothionein (MT) can bind heavy metal ions and reduce metal toxicity. Copper toxicity has become a major problem with increasing agricultural and environmental pollution. Here, we investigated the possible roles of ROS, NO and metallo...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.04.007
更新日期:2010-10-15 00:00:00
abstract::We previously isolated Nicotiana benthamiana matrix metalloprotease 1 (NMMP1) from tobacco leaves. The NMMP1 gene encodes a highly conserved, Zn-containing catalytic protease domain that functions as a factor in the plant's defense against bacterial pathogens. Expression of NMMP1 was strongly induced during interactio...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2017.08.010
更新日期:2017-11-01 00:00:00
abstract::Plants regulate a number of primary metabolites, including carbohydrates, organic acids, and amino acids, in response to UV-B radiation. Therefore, it is essential to understand the time-dependent response of rice plants to UV-B stress. This study focused on the response of plants to UV-B at different leaf development...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2018.02.007
更新日期:2018-09-01 00:00:00
abstract::Pandanus fascicularis is dioecious with the female plant producing a non-scented fruit while the male produces a flower rich in volatiles. The essential oil extracted from the flowers is economically exploited as a natural flavouring agent as well as for its therapeutic properties. Molecular dissection of this distinc...
journal_title:Journal of plant physiology
pub_type: 杂志文章
doi:10.1016/j.jplph.2010.01.008
更新日期:2010-07-15 00:00:00