Changes in sarcolemmal Ca entry and sarcoplasmic reticulum Ca content in ventricular myocytes from patients with end-stage heart failure following myocardial recovery after combined pharmacological and ventricular assist device therapy.


AIMS:Support with left ventricular assist devices (LVAD) improves cardiac performance in patients with end-stage heart failure. In some cases this strategy, combined with pharmacological treatment, has led to a clinical improvement which remained after LVAD explant. This study defines changes in Ca handling at the cellular level in failing left ventricular tissue taken at LVAD implant (LVAD core) and LVAD removal (post-LVAD). METHODS AND RESULTS:We studied cell size and Ca regulation in enzymatically dissociated cardiac myocytes. We used confocal microscopy and electrophysiological techniques to investigate the SR Ca content and major Ca movements across the sarcolemma during the action potential. We firstly recorded a significant reduction in cell capacitance and cell volume consistent with regression of cellular hypertrophy in post-LVAD myocytes compared with LVAD core myocytes. Ca entry via sarcolemmal Ca channels during the action potential using action potential voltage-clamping was significantly increased in post-LVAD myocytes compared with LVAD cores myocytes. Finally, SR Ca content (assessed by integrating the caffeine-induced Na/Ca exchanger transient inward current) in post-LVAD myocytes was also significantly increased compared with LVAD cores myocytes. CONCLUSIONS:These results show that in myocytes from patients after LVAD support there is more Ca entry to trigger Ca release and more SR Ca content, leading to improved contractile function.


Eur Heart J


European heart journal


Terracciano CM,Harding SE,Adamson D,Koban M,Tansley P,Birks EJ,Barton PJ,Yacoub MH




Has Abstract


2003-07-01 00:00:00














  • Experience of percutaneous transluminal coronary angioplasty in orthotopic cardiac transplant recipients.

    abstract::We evaluated the role of percutaneous transluminal coronary angioplasty (PTCA) in a series of orthotopic cardiac transplant recipients with severe epicardial coronary occlusive disease. Ten orthotopic cardiac transplant patients treated by PTCA up to March 1990 were reviewed. All had significant epicardial coronary ar...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Mullins PA,Shapiro LM,Aravot DA,Scott JP,Large SR,Wallwork J,Schofield PM

    更新日期:1991-11-01 00:00:00

  • Sex-dependent differences in left ventricular function and structure in chronic pressure overload.

    abstract::To evaluate gender-related differences in left ventricular (LV) structure and function in aortic stenosis, LV biplane cineangiography, micromanometry and endomyocardial biopsies were carried out in 56 patients with aortic stenosis and normal coronary arteries. Patients were divided into males (M: n = 35), and females ...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Villari B,Campbell SE,Schneider J,Vassalli G,Chiariello M,Hess OM

    更新日期:1995-10-01 00:00:00

  • Identification of a novel loss-of-function calcium channel gene mutation in short QT syndrome (SQTS6).

    abstract:AIMS:Short QT syndrome (SQTS) is a genetically determined ion-channel disorder, which may cause malignant tachyarrhythmias and sudden cardiac death. Thus far, mutations in five different genes encoding potassium and calcium channel subunits have been reported. We present, for the first time, a novel loss-of-function mu...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Templin C,Ghadri JR,Rougier JS,Baumer A,Kaplan V,Albesa M,Sticht H,Rauch A,Puleo C,Hu D,Barajas-Martinez H,Antzelevitch C,Lüscher TF,Abriel H,Duru F

    更新日期:2011-05-01 00:00:00

  • Heart rate variability and ischaemia in patients with coronary heart disease and stable angina pectoris; influence of drug therapy and prognostic value. TIBBS Investigators Group. Total Ischemic Burden Bisoprolol Study.

    abstract:AIMS:Determination of the influence of therapy with bisoprolol and nifedipine on the heart rate variability of patients from the Total Ischemic Burden Bisoprolol Study and examination of the prognostic value. METHODS AND RESULTS:Four hundred and twenty-two patients with stable angina were included. The heart rate vari...

    journal_title:European heart journal

    pub_type: 临床试验,杂志文章,多中心研究,随机对照试验


    authors: Weber F,Schneider H,von Arnim T,Urbaszek W

    更新日期:1999-01-01 00:00:00

  • Fibrinogen predicts ischaemic stroke and advanced atherosclerosis but not echolucent, rupture-prone carotid plaques: the Copenhagen City Heart Study.

    abstract:AIMS:Whether the association between fibrinogen and cardiovascular events reflects an association with advanced atherosclerosis in general, or rupture-prone plaques in particular, is unclear. We examined whether fibrinogen predicts incidence of ischaemic stroke, advanced atherosclerosis (measured as carotid artery sten...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Kofoed SC,Wittrup HH,Sillesen H,Nordestgaard BG

    更新日期:2003-03-01 00:00:00

  • Management and 6-month outcomes in elderly and very elderly patients with high-risk non-ST-elevation acute coronary syndromes: The Global Registry of Acute Coronary Events.

    abstract:AIMS:To test the hypothesis that increasing age in patients presenting with high-risk non-ST-segment elevation acute coronary syndromes (NSTE-ACS) does not adversely influence the benefit of an early invasive strategy on major adverse events at 6 months. METHODS AND RESULTS:We report clinical outcomes in young (<70), ...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Devlin G,Gore JM,Elliott J,Wijesinghe N,Eagle KA,Avezum A,Huang W,Brieger D,GRACE Investigators.

    更新日期:2008-05-01 00:00:00

  • Treatment of calcified aortic stenosis: surgery or percutaneous transluminal aortic valvuloplasty?

    abstract::A total of 546 patients with severe aortic stenosis (AS) were retrospectively reviewed to assess the efficacy of percutaneous transluminal valvuloplasty (PTV) and valve replacement (VR). Of these, 490 underwent VR between 1968 and 1986 (mean age 62 +/- 12 years, 71.7% were in NYHA class III or IV), 68.8% received mech...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Acar J,Vahanian A,Slama M,Cormier B,Michel PL,Luxereau P,Farah E,Leborgne O,Dermine P

    更新日期:1988-04-01 00:00:00

  • Prevalence and prognostic significance of cardiac arrhythmias detected by ambulatory electrocardiography in subjects 85 years of age.

    abstract::24 hour ambulatory electrocardiography was performed in a representative group of people born in 1897. Out of 73 people included in the study, 22 were without known heart disease, 15 had definite ischaemic heart disease (IHD) and 36 symptoms of possible cardiac origin. An R-R interval in excess of 2000 ms was seen in ...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Ingerslev J,Bjerregaard P

    更新日期:1986-07-01 00:00:00

  • Left ventricular outflow tract obstruction and sudden death risk in patients with hypertrophic cardiomyopathy.

    abstract:AIMS:Left ventricular outflow tract obstruction (LVOTO) is associated with reduced survival in patients with hypertrophic cardiomyopathy (HCM). The influence of LVOTO on survival from SD in relation to other recognized clinical risk markers is unknown. METHODS AND RESULTS:A total of 917 patients with HCM (554 males, 4...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Elliott PM,Gimeno JR,Tomé MT,Shah J,Ward D,Thaman R,Mogensen J,McKenna WJ

    更新日期:2006-08-01 00:00:00

  • Regional 99mTc-methoxyisobutyl-isonitrile-uptake at rest in patients with myocardial infarcts: comparison with morphological and functional parameters obtained from gradient-echo magnetic resonance imaging.

    abstract::It is not yet clear whether 99mTc-methoxyisobutyl-isonitrile (MIBI)-uptake is a reliable indicator of myocardial viability, and a threshold value, differentiating viable from scarred myocardium, in comparison to a morphological and functional standard of reference has not been defined. MIBI-uptake was quantified in 80...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Baer FM,Smolarz K,Theissen P,Voth E,Schicha H,Sechtem U

    更新日期:1994-01-01 00:00:00

  • Physiological parameters during the initial stages of cardiopulmonary exercise testing in patients with chronic heart failure. Their value in the assessment of clinical severity and prognosis.

    abstract:AIMS:To analyse the relationship between initial exercise parameters and peak oxygen consumption and prognosis in 96 patients with congestive heart failure. METHODS AND RESULTS:Comparison of responses during the initial 6 min of cardiopulmonary exercise testing (Naughton modified protocol) in patients stratified to th...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: de Vries RJ,van Veldhuisen DJ,Dunselman PH,van der Veer N,Crijns HJ

    更新日期:1997-12-01 00:00:00

  • An assessment of dobutamine echocardiography and end-diastolic wall thickness for predicting post-revascularization functional recovery in patients with chronic coronary artery disease.

    abstract:AIMS:This study investigated the functional outcome of wall-thinned, akinetic myocardium after revascularization and evaluated the accuracy of dobutamine echocardiography in predicting post-revascularization functional recovery with the assessment of end-diastolic wall thickness in chronic ischaemic patients. METHODS ...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Furukawa T,Haque T,Takahashi M,Kinoshita M

    更新日期:1997-05-01 00:00:00

  • Drug- and disease-induced changes of human cardiac beta 1- and beta 2-adrenoceptors.

    abstract::Cardiac beta-adrenoceptor density and subtype distribution has been determined in different kinds of heart failure. A decrease in cardiac beta-adrenoceptor function appears to be a general phenomenon in all kinds of heart failure. However, cardiac beta 1- and beta 2-adrenoceptors seem to be differentially affected in ...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Brodde OE,Zerkowski HR,Borst HG,Maier W,Michel MC

    更新日期:1989-06-01 00:00:00

  • Inhibitory effect of acute and chronic administration of digitalis on the sick sinus node.

    abstract::We have determined the effects of the acute administration of intravenous (i.v.) digitalis administration (0.8 mg of digoxin in 5-10 min) on the electrophysiological variables of 12 normal subjects (group 1), 12 patients with asymptomatic sinus node bradycardia (group 2) and 34 patients with symptomatic sinus node dys...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Bolognesi R,Benedini G,Ferrari R,Visioli O

    更新日期:1986-04-01 00:00:00

  • The early termination of clinical trials: causes, consequences, and control. With special reference to trials in the field of arrhythmias and sudden death. Task Force of the Working Group on Arrhythmias of the European Society of Cardiology.

    abstract::The early termination of clinical trials, for either benefit or harm, often generates undue enthusiasm or alarm. The enhanced publicity attending early termination of a trial promotes inappropriate interpretations because of the inherent difficulty of a prompt and comprehensive data review. Furthermore, the process of...

    journal_title:European heart journal

    pub_type: 杂志文章



    更新日期:1994-06-01 00:00:00

  • Effect of thrombolytic therapy on the risk of cardiac rupture and mortality in older patients with first acute myocardial infarction.

    abstract:AIMS:To evaluate the effect of thrombolysis on mortality and its causes in older patients with acute myocardial infarction (AMI). METHODS AND RESULTS:An analysis of 706 consecutive patients > or =75 years old with a first AMI enrolled in the PPRIMM75 registry showed that although there were important differences in ba...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Bueno H,Martínez-Sellés M,Pérez-David E,López-Palop R

    更新日期:2005-09-01 00:00:00

  • Long-term benefit-risk balance of drug-eluting vs. bare-metal stents in daily practice: does stent diameter matter? Three-year follow-up of BASKET.

    abstract:AIMS:To assess the long-term benefit-risk ratio of drug-eluting (DES) vs. bare-metal stents (BMS) relative to stent size. METHODS AND RESULTS:All 826 consecutive BASKET (BAsel Stent Kosten-Effektivitäts Trial) patients randomized 2:1 to DES vs. BMS were followed after 3 years. Data were analysed separately for patient...

    journal_title:European heart journal

    pub_type: 杂志文章,随机对照试验


    authors: Pfisterer M,Brunner-La Rocca HP,Rickenbacher P,Hunziker P,Mueller C,Nietlispach F,Leibundgut G,Bader F,Kaiser C,BASKET.

    更新日期:2009-01-01 00:00:00

  • Changes in cardiac function after effective treatment of hypertensive emergencies with i.v. clonidine.

    abstract::Clonidine administration by i.v. infusion in 12 patients with hypertension emergencies (diastolic blood pressure over 130 mmHg) resulted in the normalization of blood pressure (BP) in all patients. Lowering of BP was associated with a reduction in total and lower limb vascular resistance. Heart rate showed a slight an...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Masotti G,Scarti L,Poggesi L,Bisi G,Gallini C,Neri Serneri GG

    更新日期:1984-12-01 00:00:00

  • QT dispersion and RR variations on 12-lead ECGs in patients with congestive heart failure secondary to idiopathic dilated cardiomyopathy.

    abstract::Increased QT dispersion, which has been proposed as a marker of ventricular repolarization inhomogeneity, may predispose to ventricular arrhythmias. Data on QT dispersion in patients with congestive heart failure are scarce. In this study, conventional 12-lead ECGs were recorded in 135 consecutive patients with conges...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Fei L,Goldman JH,Prasad K,Keeling PJ,Reardon K,Camm AJ,McKenna WJ

    更新日期:1996-02-01 00:00:00

  • The immediate effect of nitroglycerin on total body blood volume distribution in patients with congestive heart failure: a non-invasive study.

    abstract::The effect of sublingually administered nitroglycerin (0.5 mg) on distribution of blood volume in 16 patients with congestive heart failure (NYHA class II and III), randomly assigned to placebo (N = 8) or nitroglycerin (N = 8) was studied 20 minutes after in vivo labelling of red blood cells with 99m-Tc pertechnetate....

    journal_title:European heart journal

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Schneider AJ,Teule GJ,Groeneveld AB,Nauta J,Luth WJ,Thijs LG

    更新日期:1987-10-01 00:00:00

  • The architecture of the left lateral atrial wall: a particular anatomic region with implications for ablation of atrial fibrillation.

    abstract:AIMS:We examined the left lateral ridge (LLR) between the orifices of the left pulmonary veins and the left atrial appendage for a better understanding of its structural composition relevant to ablations for atrial fibrillation (AF). METHODS AND RESULTS:The LLR and its surrounding areas were studied in 40 heart specim...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Cabrera JA,Ho SY,Climent V,Sánchez-Quintana D

    更新日期:2008-02-01 00:00:00

  • Long-term survival in severe heart failure in patients treated with enalapril. Ten year follow-up of CONSENSUS I.

    abstract:BACKGROUND:The CONSENSUS trial was the first study to show prognostic improvement by an ACE inhibitor. Patients in NYHA class IV heart failure were treated with enalapril or placebo. After study completion (average 183 days) all patients were offered open-label enalapril therapy. This paper reports on the survival at t...

    journal_title:European heart journal

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Swedberg K,Kjekshus J,Snapinn S

    更新日期:1999-01-01 00:00:00

  • Maintenance of vascular integrity: role of nitric oxide and other bradykinin mediators.

    abstract::In the blood-vessel wall, the endothelium plays a key functional role by generating several substances that modulate vascular smooth muscle tone, as well as growth, and platelet function. This review focuses on the role of the endothelial L-arginine/nitric oxide signal transduction pathway in the maintenance of vascul...

    journal_title:European heart journal

    pub_type: 杂志文章,评审


    authors: Cosentino F,Lüscher TF

    更新日期:1995-11-01 00:00:00

  • Prognostic role of echocardiography and brain natriuretic peptide in symptomatic breathless patients in the community.

    abstract:AIMS:Brain natriuretic peptide (BNP), left ventricular (LV) systolic function, and mitral filling pattern (MFP) are prognostic indicators in patients with heart failure (HF). This study evaluated the potential role of E/Ea for predicting cardiovascular (CV) events in patients with suspected HF. This non-invasive measur...

    journal_title:European heart journal

    pub_type: 杂志文章,随机对照试验


    authors: Whalley GA,Wright SP,Pearl A,Gamble GD,Walsh HJ,Richards M,Doughty RN

    更新日期:2008-02-01 00:00:00

  • Utilization of coronary angioplasty and cost of angioplasty disposables in 14 western European countries. European Angioplasty Survey Group.

    abstract::An inventory has been made of the practice of angioplasty in 14 European countries from 1985 onwards. The numbers of procedures performed varied in 1985 from 3 to 186 per million inhabitants, in 1991 from 52 to 716. All countries showed a steady increase, but the highest performer. Belgium, achieved only 55% of the nu...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: van den Brand M

    更新日期:1993-03-01 00:00:00

  • Creatine kinase-MB elevation after percutaneous coronary intervention predicts adverse outcomes in patients with acute coronary syndromes.

    abstract:AIM:To study the relationship between outcomes and peak creatine kinase (CK)-MB levels after percutaneous coronary intervention (PCI) in patients with non-ST-segment elevation acute coronary syndromes (NSTE ACS). METHODS AND RESULTS:Peak CK-MB ratios (peak CK-MB level/upper limit of normal [ULN]) after PCI were analys...

    journal_title:European heart journal

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Roe MT,Mahaffey KW,Kilaru R,Alexander JH,Akkerhuis KM,Simoons ML,Harrington RA,Tardiff BE,Granger CB,Ohman EM,Moliterno DJ,Lincoff AM,Armstrong PW,Van de Werf F,Califf RM,Topol EJ

    更新日期:2004-02-01 00:00:00

  • Unprotected left main revascularization in patients with acute coronary syndromes.

    abstract:AIMS:In acute coronary syndromes (ACS), the optimal revascularization strategy for unprotected left main coronary disease (ULMCD) has been little studied. The objectives of the present study were to describe the practice of ULMCD revascularization in ACS patients and its evolution over an 8-year period, analyse the pro...

    journal_title:European heart journal

    pub_type: 杂志文章,多中心研究


    authors: Montalescot G,Brieger D,Eagle KA,Anderson FA Jr,FitzGerald G,Lee MS,Steg PG,Avezum A,Goodman SG,Gore JM,GRACE Investigators.

    更新日期:2009-10-01 00:00:00

  • Functional characteristics of myocardial bridging. A combined angiographic and intracoronary Doppler flow study.

    abstract:AIMS:Combined quantitative coronary angiography and intracoronary Doppler flow velocity measurements were performed to study the underlying haemodynamic mechanisms leading to myocardial ischaemia in patients with myocardial bridging in the absence of coronary artery disease. METHODS AND RESULTS:In 42 symptomatic patie...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Schwarz ER,Klues HG,vom Dahl J,Klein I,Krebs W,Hanrath P

    更新日期:1997-03-01 00:00:00

  • Clinical and prognostic profile of patients with infective endocarditis who need urgent surgery.

    abstract:AIMS:Surgery in patients with infective endocarditis (IE) can be elective (upon completion of antibiotic treatment) or urgent (before antibiotic treatment has ended) when the clinical course is unfavourable. However, urgent surgery for left-sided endocarditis is associated with high mortality. The aims of this study we...

    journal_title:European heart journal

    pub_type: 杂志文章


    authors: Revilla A,López J,Vilacosta I,Villacorta E,Rollán MJ,Echevarría JR,Carrascal Y,Di Stefano S,Fulquet E,Rodríguez E,Fiz L,San Román JA

    更新日期:2007-01-01 00:00:00

  • Acute effects of angiotensin-converting enzyme inhibition on coronary vasomotion in hypertensive patients.

    abstract::Coronary vasodilator responses to pharmacological (acetylcholine) and physiological (cold pressor test and flow dependent dilation) stimuli are impaired in hypertensive patients. The ability of angiotensin-converting enzyme inhibitors to restore normal coronary responses in hypertension and the potential mechanisms in...

    journal_title:European heart journal

    pub_type: 杂志文章,评审


    authors: Nitenberg A,Antony I

    更新日期:1998-09-01 00:00:00