Abstract：
:Four different fluorochromes were injected into adjacent cervical spinal cord segments, 1 unique tracer per segment. Each tracer, Fluoro-Gold, Fast Blue, Diamidino Yellow dihydrochloride and Propidium Iodide, was taken up by axonal terminals and transported intra-axonally in a retrograde direction to the cell bodies. Some, though by no means all, of these axons were stem axons with terminals in 2, 3 or 4 of the injected spinal segments. Hence as many as 4 different fluorescent tracers could be discerned simultaneously within individual neuronal somata of origin using fluorescent microscopy. These results extend the possibilities for multiple interconnection determinations within the central nervous system. Specifically, the potential for individual neurons of a nucleus to project collateral branches of a stem axon to as many as 4 different central nervous system nuclei now can be studied simultaneously using these 4 fluorescent tracers.
journal_name
J Neurosci Methodsjournal_title
Journal of neuroscience methodsauthors
Akintunde A,Buxton DFdoi
10.1016/0165-0270(92)90039-gsubject
Has Abstractpub_date
1992-10-01 00:00:00pages
15-22issue
1-2eissn
0165-0270issn
1872-678Xpii
0165-0270(92)90039-Gjournal_volume
45pub_type
杂志文章abstract：BACKGROUND:The construction of multichannel micro-electrode arrays (MEA) generally requires complex and expensive procedures. Here we describe a simple, cheap, flexible method of linear MEA construction. NEW METHOD:Our method allows manufacture of linear MEA (cross section ∼ 375 × 250 μm with 12 electrodes) of any des...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.109011
更新日期：2021-01-15 00:00:00
abstract：:Phalen's test, with more than 100 degrees of elbow flexion, is not always comfortable for patients with elbow pathology. Therefore, we suggest a modification of the test with the patient having both arms resting on a pillow or arm-holders in a relaxed position, the hands floating at the end, and the examiner passively...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2007.12.019
更新日期：2008-05-15 00:00:00
abstract：:The biological and clinical relevance of axonal transport has driven the development of a variety of new approaches to its study, including the generation of fluorescence or brightfield movies of moving cargoes within axons. Kymograph analysis is a simple and effective tool used to analyze axonal transport in neurons....
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.05.013
更新日期：2011-08-15 00:00:00
abstract：:A method is described for the construction of simple, robust electrodes suitable for obtaining long-term chronic recordings of electromyogram activity from delicate muscles of small experimental animals. The electrodes are equipped with small, gold-plated barbed tips which have the form of harpoons that may be pushed ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(90)90081-p
更新日期：1990-07-01 00:00:00
abstract：:The electrochemical technique of differential pulse voltammetry (DPV) with micro-biosensors has been used for a number of years to monitor in vivo and in situ changes in the extracellular concentration of cerebral ascorbic acid, as well as that of the metabolites of dopamine (DA) and serotonin (5-HT). We have recently...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90094-t
更新日期：1992-05-01 00:00:00
abstract：BACKGROUND:The misallocation of treatment-variance on the wrong component has been discussed in the context of temporal principal component analysis of event-related potentials. There is, until now, no rotation-method that can perfectly recover Wood and McCarthy's prototypes without making use of additional information...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.11.011
更新日期：2018-02-01 00:00:00
abstract：:Hydraulically coupled volume microejection (HCVM) was employed to overcome known drawbacks of conventional pressure ejection of drugs in isolated brain slices and other tissues. For HCVM, a solution-filled glass micropipette is connected to a motor-driven, liquid-filled syringe by low-compliance, liquid-filled tubing,...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2006.01.007
更新日期：2006-09-15 00:00:00
abstract：:Over the past few years there has been an increased interest in studying the underlying neural mechanism of attention and cognitive brain activity. This paper aims towards identifying and analyzing distinct responses in an auditory working memory paradigm, as independent components with variable latency, frequency and...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2011.02.001
更新日期：2011-04-30 00:00:00
abstract：:A new simple technique for the accurate modification of microelectrode and micropipette tips by breaking has been developed. The breaking is done by longitudinally inserting a micropipette into a larger previously broken pipette for stability and by applying a force on the micropipette to cause it to break. Tips will ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(83)90106-1
更新日期：1983-09-01 00:00:00
abstract：:We describe a novel method for retrogradely labeling specific neuronal populations using voltage-sensitive dyes. Styryl dyes were injected into the ventral roots of the isolated embryonic chick spinal cord. After waiting several hours, the dye labeled motoneurons and autonomic preganglionic neurons. Neuronal cell bodi...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(96)00108-2
更新日期：1996-12-28 00:00:00
abstract：:We describe a visual assay of neuronal electrophysiologic status for use with cultured neurons, based on the exclusion of propidium iodide (PI) by intact cellular membranes. We use this fluorescent dye, which binds to nucleic acids, at concentrations suitable for long-term exposure to neurons without toxicity. We corr...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(90)90007-3
更新日期：1990-01-01 00:00:00
abstract：BACKGROUND:The human auditory brainstem frequency-following response (FFR) is an objective measure used to investigate the brainstem's encoding ability of sounds. Traditionally, FFRs are recorded under close-field conditions (earphones), but free-field stimulations (loudspeaker) have yet to be attempted, which would in...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2017.01.016
更新日期：2017-03-15 00:00:00
abstract：:Studies of the neural control of movement often require or benefit from long-term recording of EMG activity from large numbers of muscles involved in a particular motor task. While chronic recording of EMG activity has been described in a number of previous monkey studies, the number of muscles recorded has been somew...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00155-2
更新日期：2000-03-15 00:00:00
abstract：:A microfluidic chip for culturing neurons and spatially isolating axons from somas is presented for use with visually guided whole-cell electrophysiological measurements. A modular design consisting of detachable and re-sealable layers is used to satisfy the requirements of both long-term neuron culturing as well as e...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2012.10.013
更新日期：2013-01-30 00:00:00
abstract：BACKGROUND:Despite extensive environmental standardization and the use of genetically and microbiologically defined mice of similar age and sex, individuals of the same mouse inbred strain commonly differ in quantitative traits. This is a major issue as it affects the quality of experimental results. Standard analysis ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2020.108810
更新日期：2020-09-01 00:00:00
abstract：:A computer model of the hippocampal CA1 area, which receives synaptic inputs from CA3 neurons via the Schaffer collaterals, was constructed. Pyramidal cells (PC) and two types of interneurons were represented by compartmental models, and mechanisms of feed-forward inhibition (FFI) and recurrent inhibition were incorpo...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(00)00334-4
更新日期：2001-01-15 00:00:00
abstract：:Several neuronal tracing substances were applied to the cut ends of leech cephalic nerves and the resulting backfills into the subesophageal ganglion (sbEG) were mapped. A 12 h incubation in 3 kDa dextrans conjugated either to a fluorochrome or to biotin (subsequently tagged with peroxidase) was satisfactory. In separ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：
更新日期：1996-08-01 00:00:00
abstract：:In the last few years quite powerful complete computer systems have become available that fill many laboratory needs at low cost (US $3000-10,000). This development is based on the use of microcomputers, which now have capabilities that were previously associated with minicomputers. Due to the rapidly expanding use of...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(82)90044-9
更新日期：1982-01-01 00:00:00
abstract：:Cobalamin (Cbl) utilization as a cofactor for methionine synthase and methylmalonyl-CoA mutase is dependent on the transport of Cbl through lysosomes and its subsequent delivery to the cytosol and mitochondria. We speculated that neuropathological conditions that impair lysosomal function (e.g., age-related lipofuscin...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.04.008
更新日期：2013-07-15 00:00:00
abstract：:Matrix metalloproteinases (MMPs) play an important role in the pathogenesis of ischaemic stroke. In particular, the mature forms of MMPs 2 and 9 have similar sizes and share gelatine as a common substrate. Both MMPs are upregulated in ischaemic stroke and play detrimental roles during stroke pathogenesis. Throughout t...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.03.007
更新日期：2013-05-30 00:00:00
abstract：:We have attempted to demonstrate the technical requirements and performance of a microscopical assay using a densitometric application of image analysis to measure immunohistochemical stain intensity. Not surprisingly, the techniques required were more demanding than those used for the quantification of field and obje...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(92)90048-i
更新日期：1992-10-01 00:00:00
abstract：:A sensitive procedure for simultaneous determination of in vivo rates of tryptophan hydroxylation and levels of serotonin (5-HT) in discrete rat brain nuclei is described. Rates of tryptophan hydroxylation are estimated by 5-hydroxytryptophan (5-HTP) accumulation following l-aromatic amino acid decarboxylase inhibitio...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(82)90015-2
更新日期：1982-07-01 00:00:00
abstract：:Functional magnetic resonance imaging (FMRI) can provide detailed images of human brain that reflect localized changes in cerebral blood flow and oxygenation induced by sensory, motor, or cognitive tasks. This review presents methods for gradient-recalled echo-planar functional magnetic resonance imaging (FMRI). Also ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/0165-0270(94)90191-0
更新日期：1994-10-01 00:00:00
abstract：:Electrical potentials recorded extracellularly from the sinus gland of the isopod, Oniscus asellus, were stored on video tape with the aid of a digital-audio (DA) processor and a video cassette recorder (VCR). The DA processor transforms the analog signal to digital pulses of equal amplitude and converts these pulses ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(85)90058-5
更新日期：1985-10-01 00:00:00
abstract：BACKGROUND:Current assessment of sport-related concussion involves a multifaceted approach including neurocognitive testing, symptom report, and balance assessment. Lacking from this paradigm is a truly objective measure of the physiological changes associated with concussion. NEW METHOD:A device called the Brain Acou...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2013.09.003
更新日期：2014-01-15 00:00:00
abstract：BACKGROUND:PET allows the measurement of CBF, CBV and CMRO2 in human and plays an important role in the diagnosis of pathologic conditions and clinical research. On the other hand, in animal studies, there is no optical imaging system for evaluating changes in CBF and CBV, and oxygen metabolism, from the same brain are...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2014.08.022
更新日期：2014-11-30 00:00:00
abstract：:The neocortex is the site of origin of several forms of acquired epilepsy. Here we provide a brief review of experimental models that were recently developed to study neocortical epileptogenesis as well as some major results obtained with these methods. Most of neocortical seizures appear to be nocturnal and it is kno...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章,评审
doi：10.1016/j.jneumeth.2015.08.030
更新日期：2016-02-15 00:00:00
abstract：:We describe the fabrication and use of a new type of extracellular micro-electrode array mounted on a flexible transparent polyimide substrate that can be rapidly moved from one part of a culture of vertebrate neurons (rat nodose) to another, which permits co-culture of glia under the neurons and is easily and rapidly...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/s0165-0270(01)00509-x
更新日期：2002-02-15 00:00:00
abstract：:Attempts to correlate the amount of bilateral brain injury with behavioral changes can be complicated if the lesions are bilaterally unsymmetrical in volume. Further complications are introduced if unilateral lesions are ineffective in producing behavioral changes. An index, W%, is proposed which is based on the produ...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/0165-0270(84)90046-3
更新日期：1984-11-01 00:00:00
abstract：BACKGROUND:Automated segmentation of brain structures is an important task in structural and functional image analysis. We developed a fast and accurate method for the striatum segmentation using deep convolutional neural networks (CNN). NEW METHOD:T1 magnetic resonance (MR) images were used for our CNN-based segmenta...
journal_title：Journal of neuroscience methods
pub_type： 杂志文章
doi：10.1016/j.jneumeth.2016.10.007
更新日期：2016-12-01 00:00:00
