Abstract:
:The present article reviews interesting cationic liposomes (cationic transfection lipids) with novel cationic cholesterol derivatives, a new strategy in gene transfection developed by our group and the presently accepted molecular mechanism of gene transfection. Use of confocal laser scanning microscopy and atomic force microscopy in elucidating the molecular mechanism of gene transfection by cationic liposomes is also reviewed using examples from our own work. As delineated below, both the confocal laser scanning microscopic and the atomic force microscopic results advocate for the involvement of the sequential three steps in gene transfection mediated by the cationic liposomes: endocytotic internalization of the lipoplexes (liposome-DNA complexes) into the target cells, endosome-lysosome fusion whereby the DNA gets released from the liposomes and moves towards the nucleus of the target cells and microtubule organization apparently involved in trafficking the transfected foreign genes to lysosomes. Furthermore, the present article also reviews couple of important strategies in gene transfection namely, use of liposomes made from biosurfactants and harnessing efficient gene transfection by activating the membrane-bound receptor molecules.
journal_name
Curr Med Chemjournal_title
Current medicinal chemistryauthors
Nakanishi Mdoi
10.2174/0929867033457395subject
Has Abstractpub_date
2003-07-01 00:00:00pages
1289-96issue
14eissn
0929-8673issn
1875-533Xjournal_volume
10pub_type
杂志文章,评审abstract::The lung is a unique organ in terms of its direct exposure to high levels of oxygen and reactive compounds. Several parenchymal lung diseases (e.g. emphysema associated with smoking and a number of fibrotic lung disorders) have been proposed to be due to the exposure of the lung to exogenous irritants leading to local...
journal_title:Current medicinal chemistry
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journal_title:Current medicinal chemistry
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journal_title:Current medicinal chemistry
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journal_title:Current medicinal chemistry
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journal_title:Current medicinal chemistry
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journal_title:Current medicinal chemistry
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journal_title:Current medicinal chemistry
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abstract::More than five decades since the Discovery of Penicillin, the chemistry and biological activity of b-lactams continue to attract the wide spread attention of research workers. Owing to high efficacy and extremely safe toxicological profile, they are agents of choice in the current therapeutic index for the bacterial i...
journal_title:Current medicinal chemistry
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更新日期:1999-03-01 00:00:00
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更新日期:2010-01-01 00:00:00
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更新日期:2017-01-01 00:00:00
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更新日期:1998-10-01 00:00:00
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journal_title:Current medicinal chemistry
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更新日期:2008-01-01 00:00:00
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更新日期:2020-01-01 00:00:00
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更新日期:2013-01-01 00:00:00
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journal_title:Current medicinal chemistry
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更新日期:2014-01-01 00:00:00
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更新日期:2009-01-01 00:00:00