Time course of seroconversion by HerpeSelect ELISA after acquisition of genital herpes simplex virus type 1 (HSV-1) or HSV-2.

Abstract:

BACKGROUND:HerpeSelect HSV-1 and HSV-2 ELISAs are glycoprotein G-based, type-specific antibody detection tests that are approved by the US Food and Drug Administration for diagnosis of genital herpes. GOAL:The goal was to determine seroconversion times by means of HerpeSelect ELISAs. STUDY DESIGN:Four-hundred thirteen sera from 113 patients with recently acquired genital herpes were tested by HerpeSelect ELISAs and Western blot (WB). Thirty-one patients had primary genital HSV-1 (group 1), 56 had primary HSV-2 (group 2), and 26 had prior HSV-1 antibodies and newly acquired HSV-2 (group 3). RESULTS:Median interval from onset of symptoms to seroconversion was 25 days, as determined by HerpeSelect HSV-1, versus 33 days by WB for group 1; 21 days by HerpeSelect HSV-2 versus 40 days by WB (group 2; P = 0.0005); and 23 days by HerpeSelect HSV-2 ELISA versus 47 days by WB (group 3; P = 0.02). In long-term follow-up, transient reversion to HerpeSelect negativity occurred in 3 of 31 HSV-1-infected subjects (10%) and in 2 of 82 HSV-2-infected subjects (2%). CONCLUSION:Seroconversion to HSV-2 was determined faster by HerpeSelect than by WB.

journal_name

Sex Transm Dis

authors

Ashley-Morrow R,Krantz E,Wald A

doi

10.1097/00007435-200304000-00007

subject

Has Abstract

pub_date

2003-04-01 00:00:00

pages

310-4

issue

4

eissn

0148-5717

issn

1537-4521

pii

00007435-200304000-00007

journal_volume

30

pub_type

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