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Directed evolution of N-acetylneuraminic acid aldolase to catalyze enantiomeric aldol reactions.

Abstract：

:Expanding the scope of substrate specificity and stereoselectivity is of current interest in enzyme catalysis. Using error-prone PCR for in vitro directed evolution, the Neu5Ac aldolase from Escherichia coli has been altered to improve its catalytic activity toward enantiomeric substrates including N-acetyl-L-mannosamine and L-arabinose to produce L-sialic acid and L-KDO, the mirror-image sugars of the corresponding naturally occurring D-sugars. The first generation variant containing two mutations (Tyr98His and Phe115Leu) outside the (alpha,beta)(8)-barrel active site exhibits an inversion of enantioselectivity toward KDO and the second generation variant contains an additional amino acid change Val251Ile outside the alpha,beta-barrel active site that improves the enantiomeric formation of L-sialic acid and L-KDO. The X-ray structure of the triple mutant epNanA.2.5 at 2.3A resolution showed no significant difference between the wild-type and the mutant enzymes. We probed the potential structural 'hot spot' of enantioselectivity with saturation mutagenesis at Val251, the mutated residue most proximal to the Schiff base forming Lys165. The selected variant had an increase in k(cat) via replacement with another hydrophobic residue, leucine. Further sampling of a larger sequence space with error-prone PCR selected a third generation variant with significant improvement in L-KDO catalysis and a complete reversal of enantioselectivity.

journal_name

Bioorg Med Chem

journal_title

Bioorganic & medicinal chemistry

authors

Wada M,Hsu CC,Franke D,Mitchell M,Heine A,Wilson I,Wong CH

doi

10.1016/s0968-0896(03)00052-x

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

2091-8

issue

9

eissn

0968-0896

issn

1464-3391

pii

S096808960300052X

journal_volume

11

pub_type

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