Bioassays based on molecular nanomechanics.

Abstract:

:Recent experiments have shown that when specific biomolecular interactions are confined to one surface of a microcantilever beam, changes in intermolecular nanomechanical forces provide sufficient differential torque to bend the cantilever beam. This has been used to detect single base pair mismatches during DNA hybridization, as well as prostate specific antigen (PSA) at concentrations and conditions that are clinically relevant for prostate cancer diagnosis. Since cantilever motion originates from free energy change induced by specific biomolecular binding, this technique is now offering a common platform for label-free quantitative analysis of protein-protein binding, DNA hybridization DNA-protein interactions, and in general receptor-ligand interactions. Current work is focused on developing "universal microarrays" of microcantilever beams for high-throughput multiplexed bioassays.

journal_name

Dis Markers

journal_title

Disease markers

authors

Majumdar A

doi

10.1155/2002/856032

subject

Has Abstract

pub_date

2002-01-01 00:00:00

pages

167-74

issue

4

eissn

0278-0240

issn

1875-8630

journal_volume

18

pub_type

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