Abstract：
:A genome-wide computational screen was performed to identify tumor-associated alternative RNA splicing isoforms. A BLAST algorithm was used to compare 11,014 genes from RefSeq with 3,471,822 human expressed sequence tag sequences. The screen identified 26,258 alternative splicing isoforms of which 845 were significantly associated with human cancer, and 54 were specifically associated with liver cancer. Furthermore, canonical GT-AG splice junctions were used significantly less frequently in the alternative splicing isoforms in tumors. Reverse transcription-PCR experiments confirmed association of the alternative splicing isoforms with tumors. These results suggest that alternative splicing may have potential as a diagnostic marker for cancer.
journal_name
Cancer Resjournal_title
Cancer researchauthors
Wang Z,Lo HS,Yang H,Gere S,Hu Y,Buetow KH,Lee MPsubject
Has Abstractpub_date
2003-02-01 00:00:00pages
655-7issue
3eissn
0008-5472issn
1538-7445journal_volume
63pub_type
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