Proteome analysis of Escherichia coli K-12 by two-dimensional native-state chromatography and MALDI-MS.

abstract：:Protein translocation across the bacterial cytoplasmic membrane has been studied extensively in Escherichia coli. The identification of the components involved and subsequent reconstitution of the purified translocation reaction have defined the minimal constituents that allowed extensive biochemical characterization ...

journal_title：Molecular microbiology

authors： Manting EH,Driessen AJ

更新日期：2000-07-01 00:00:00

abstract：:Virstatin is a previously described small molecule inhibitor of Vibrio cholerae virulence. We have demonstrated that the molecule inhibits the activity of the transcriptional activator ToxT, thereby preventing elaboration of the toxin co-regulated pilus (TCP) and cholera toxin in vitro and in vivo in O1 strains of V. ...

journal_title：Molecular microbiology

authors： Shakhnovich EA,Sturtevant D,Mekalanos JJ

更新日期：2007-12-01 00:00:00

abstract：:The mechanistic details of the pathogenesis of Chlamydia, an obligate intracellular pathogen of global importance, have eluded scientists due to the scarcity of traditional molecular genetic tools to investigate this organism. Here we report a chemical biology strategy that has uncovered the first essential protease f...

journal_title：Molecular microbiology

authors： Gloeckl S,Ong VA,Patel P,Tyndall JD,Timms P,Beagley KW,Allan JA,Armitage CW,Turnbull L,Whitchurch CB,Merdanovic M,Ehrmann M,Powers JC,Oleksyszyn J,Verdoes M,Bogyo M,Huston WM

更新日期：2013-08-01 00:00:00

abstract：:In the intracellular pathogen Brucella abortus, the general stress response (GSR) signalling system determines survival under acute stress conditions in vitro, and is required for long-term residence in a mammalian host. To date, the identity of the Brucella sensor kinase(s) that function to perceive stress and direct...

journal_title：Molecular microbiology

authors： Kim HS,Willett JW,Jain-Gupta N,Fiebig A,Crosson S

更新日期：2014-11-01 00:00:00

abstract：:Burkholderia mallei, the aetiologic agent of glanders, causes a variety of illnesses in animals and humans ranging from occult infections to acute fulminating septicaemias. To better understand the role of lipopolysaccharide (LPS) in the pathogenesis of these diseases, studies were initiated to characterize the struct...

journal_title：Molecular microbiology

authors： Brett PJ,Burtnick MN,Snyder DS,Shannon JG,Azadi P,Gherardini FC

更新日期：2007-01-01 00:00:00

abstract：:Cell division in most bacteria is directed by FtsZ, a conserved tubulin-like GTPase that assembles forming the cytokinetic Z-ring and constitutes a target for the discovery of new antibiotics. The developmental regulator MciZ, a 40-amino acid peptide endogenously produced during Bacillus subtilis sporulation, halts cy...

journal_title：Molecular microbiology

authors： Araújo-Bazán L,Huecas S,Valle J,Andreu D,Andreu JM

更新日期：2019-04-01 00:00:00

abstract：:Fungal ribotoxins, such as mitogillin and the related Aspergillus toxins restrictocin and alpha-sarcin, are highly specific ribonucleases, which inactivate the ribosome enzymatically by cleaving the eukaryotic 28S RNA of the large ribosomal subunit at a single phosphodiester bond. The site of cleavage occurs between G...

journal_title：Molecular microbiology

authors： Kao R,Shea JE,Davies J,Holden DW

更新日期：1998-08-01 00:00:00

abstract：:Viable mutations affecting the 5'-phosphate sensor of RNase E, including R169Q or T170A, become lethal when combined with deletions removing part of the non-catalytic C-terminal domain of RNase E. The phosphate sensor is required for efficient autoregulation of RNase E synthesis as RNase E R169Q is strongly overexpres...

journal_title：Molecular microbiology

authors： Garrey SM,Mackie GA

更新日期：2011-06-01 00:00:00

abstract：:Two papers in this issue of Molecular Microbiology from Carol Gross and colleagues describe experiments that investigate how two alternative Escherichia coli sigma proteins recognize target promoter -10 regions. A combination of genetics, biochemistry and bioinformatics is used to show that determinants in two adjacen...

journal_title：Molecular microbiology

authors： Busby SJ

更新日期：2009-05-01 00:00:00

abstract：:Although SsrA(tmRNA)-mediated trans-translation is thought to maintain the translation capacity of bacterial cells by rescuing ribosomes stalled on messenger RNA lacking an in-frame stop codon, single disruption of ssrA does not crucially hamper growth of Escherichia coli. Here, we identified YhdL (renamed ArfA for al...

journal_title：Molecular microbiology

authors： Chadani Y,Ono K,Ozawa S,Takahashi Y,Takai K,Nanamiya H,Tozawa Y,Kutsukake K,Abo T

更新日期：2010-11-01 00:00:00

abstract：:Bacterial cytokinesis is orchestrated by an assembly of essential cell division proteins that form a supramolecular structure known as the divisome. DivIB and its orthologue FtsQ are essential members of the divisome in Gram-positive and Gram-negative bacteria respectively. DivIB is a bitopic membrane protein composed...

journal_title：Molecular microbiology

authors： Wadsworth KD,Rowland SL,Harry EJ,King GF

更新日期：2008-03-01 00:00:00

abstract：:The extracellular polysaccharide capsule is an essential virulence factor of Neisseria meningitidis, a leading cause of severe bacterial meningitis and sepsis. Serogroup B strains, the primary disease causing isolates in Europe and America, are encapsulated in alpha-2,8 polysialic acid (polySia). The capsular polymer ...

journal_title：Molecular microbiology

authors： Freiberger F,Claus H,Günzel A,Oltmann-Norden I,Vionnet J,Mühlenhoff M,Vogel U,Vann WF,Gerardy-Schahn R,Stummeyer K

更新日期：2007-09-01 00:00:00

abstract：:Gut bacteria recognize accessible glycan substrates within a complex environment. Carbohydrate binding modules (CBMs) of cell surface glycoside hydrolases often drive binding to the target substrate. Eubacterium rectale, an important butyrate-producing organism in the gut, consumes a limited range of substrates, inclu...

journal_title：Molecular microbiology

authors： Cockburn DW,Suh C,Medina KP,Duvall RM,Wawrzak Z,Henrissat B,Koropatkin NM

更新日期：2018-01-01 00:00:00

abstract：:N15 is a bacteriophage of Escherichia coli that resembles lambda, but, unlike lambda, it lysogenizes as a linear plasmid. We show that stable maintenance of this unusual plasmid-prophage depends on the parA and parB genes, relatives of the partition genes of F and P1 plasmids. ParB of N15, like its F- and P1-encoded h...

journal_title：Molecular microbiology

authors： Grigoriev PS,Lobocka MB

更新日期：2001-10-01 00:00:00

abstract：:The bacterial RNA-binding protein Hfq functions in post-transcriptional regulation of gene expression. There is evidence in a range of bacteria for specific subcellular localization of Hfq; however, the mechanism and role of Hfq localization remain unclear. Cyanobacteria harbour a subfamily of Hfq that is structurally...

journal_title：Molecular microbiology

authors： Schuergers N,Ruppert U,Watanabe S,Nürnberg DJ,Lochnit G,Dienst D,Mullineaux CW,Wilde A

更新日期：2014-05-01 00:00:00

abstract：:Quinone-based compounds have been exploited to treat infectious diseases and cancer, with such chemicals often functioning as inhibitors of key metabolic pathways or as prodrugs. Here, we screened an aziridinyl 1,4-benzoquinone (ABQ) library against the causative agents of trypanosomiasis, and cutaneous leishmaniasis,...

journal_title：Molecular microbiology

authors： Meredith EL,Kumar A,Konno A,Szular J,Alsford S,Seifert K,Horn D,Wilkinson SR

更新日期：2017-10-01 00:00:00

abstract：:NADPH oxidases (Nox) produce reactive oxygen species (ROS) in multicellular eukaryotic organisms. They trigger defense reactions ('oxidative burst') - in phagocytes and plant cells -, and are involved in a broad range of differentiation processes. Fungal Nox-complexes play a central role in vegetative, sexual and path...

journal_title：Molecular microbiology

authors： Marschall R,Tudzynski P

更新日期：2016-07-01 00:00:00

abstract：:In order to reveal functional properties of recombination involving short ssDNAs in hyperthermophilic archaea, we evaluated oligonucleotide-mediated transformation (OMT) in Sulfolobus acidocaldarius and Escherichia coli as a function of the molecular properties of the ssDNA substrates. Unmodified ssDNAs as short as 20...

journal_title：Molecular microbiology

authors： Grogan DW,Stengel KR

更新日期：2008-09-01 00:00:00

abstract：:In many streptococci, quorum sensing utilizes secreted, linear peptides that engage cognate receptors to coordinate gene expression among members of a local population. Streptococcus mutans employs the secreted peptides CSP and XIP to stimulate production of antimicrobial bacteriocins and to induce development of comp...

journal_title：Molecular microbiology

authors： Federle MJ,Morrison DA

更新日期：2012-10-01 00:00:00

abstract：:The classic expression pattern of the Fis global regulatory protein during batch culture consists of a high peak in the early logarithmic phase of growth, followed by a sharp decrease through mid-exponential growth phase until Fis is almost undetectable at the end of the exponential phase. We discovered that this patt...

journal_title：Molecular microbiology

authors： O Cróinín T,Dorman CJ

更新日期：2007-10-01 00:00:00

abstract：:A segment of the exotoxin A gene of Pseudomonas aeruginosa, coding for the N-terminal end of domain I and domain II of the toxin (ETA), was genetically fused to the diphtheria toxin gene of Corynebacterium diphtheriae, coding for the N-terminal end of A fragment of diphtheria toxin (DT). The resulting hybrid protein (...

journal_title：Molecular microbiology

authors： Guidi-Rontani C

更新日期：1992-05-01 00:00:00

abstract：:Advances in automated fluorescence microscopy have made snapshot and time-lapse imaging of bacterial cells commonplace, yet fundamental challenges remain in analysis. The vast quantity of data collected in high-throughput experiments requires a fast and reliable automated method to analyze fluorescence intensity and l...

journal_title：Molecular microbiology

authors： Cass JA,Stylianidou S,Kuwada NJ,Traxler B,Wiggins PA

更新日期：2017-03-01 00:00:00

abstract：:Trypanosoma cruzi, the etiologic agent of Chagas disease, has a complex life cycle in which four distinct developmental forms alternate between the insect vector and the mammalian host. It is assumed that replicating epimastigotes present in the insect gut are not infective to mammalian host, a paradigm corroborated b...

journal_title：Molecular microbiology

authors： Kessler RL,Contreras VT,Marliére NP,Aparecida Guarneri A,Villamizar Silva LH,Mazzarotto GACA,Batista M,Soccol VT,Krieger MA,Probst CM

更新日期：2017-06-01 00:00:00

abstract：:The human stomach pathogen Helicobacter pyloriattaches to healthy and inflamed gastric tissue through members of a paralogous family of 'Helicobacter outer membrane proteins' (Hops), including adhesins BabA, SabA, HopQ, LabA and HopZ. Hops share a conserved 25 kDa C-terminal region that is thought to form an autotrans...

journal_title：Molecular microbiology

authors： Coppens F,Castaldo G,Debraekeleer A,Subedi S,Moonens K,Lo A,Remaut H

更新日期：2018-10-01 00:00:00

abstract：:The Helicobacter pylori vacuolating cytotoxin (VacA) induces degenerative vacuolation of sensitive mammalian cell lines. Although evidence is accumulating that VacA enters cells and functions from an intracellular site of action, the biochemical mechanism by which VacA mediates cellular vacuolation has not been establ...

journal_title：Molecular microbiology

authors： Ye D,Blanke SR

更新日期：2002-03-01 00:00:00

abstract：:In the corn smut fungus Ustilago maydis, pathogenic development is controlled by the b mating type locus that encodes the two homeodomain proteins bE and bW. A heterodimer of bE and bW controls a large set of genes, either directly by binding to cis regulatory sequences or indirectly via a b-dependent regulatory casca...

journal_title：Molecular microbiology

authors： Reichmann M,Jamnischek A,Weinzierl G,Ladendorf O,Huber S,Kahmann R,Kämper J

更新日期：2002-11-01 00:00:00

abstract：:Arginine auxotrophs of the dinitrogen-fixing cyanobacterium Anabaena species strain PCC 7120 were isolated after ultraviolet light mutagenesis and penicillin enrichment. Two of these auxotrophs were complemented by a cosmid gene library of the wild-type strain established in Escherichia coli that was transferred en ma...

journal_title：Molecular microbiology

authors： Floriano B,Herrero A,Flores E

更新日期：1992-08-01 00:00:00

abstract：:We have shown previously that some particular mutations in bacteriophage Mu repressor, the frameshift vir mutations, made the protein very sensitive to the Escherichia coli ATP-dependent Clp protease. This enzyme is formed by the association between a protease subunit (ClpP) and an ATPase subunit. ClpA, the best chara...

journal_title：Molecular microbiology

authors： Mhammedi-Alaoui A,Pato M,Gama MJ,Toussaint A

更新日期：1994-03-01 00:00:00

abstract：:Pseudomonas aeruginosa chronically colonizing the lungs of cystic fibrosis (CF) patients undergoes fast evolution leading to clonal divergence. More than half of the genotypes of P. aeruginosa clone C isolates exclusively from CF lung infection exhibit large chromosomal inversions (LCIs). To analyse the impact of LCIs...

journal_title：Molecular microbiology

authors： Kresse AU,Dinesh SD,Larbig K,Römling U

更新日期：2003-01-01 00:00:00

abstract：:A fragment of mitochondrial DNA containing the Kluyveromyces lactis gene for valine-tRNA (tRNAVAL) was isolated as a multicopy suppressor of a respiratory-deficient mutant of this yeast. The mutant produced a truncated Cox14p because of a +1 frameshift mutation in COX14, a nuclear gene encoding a protein imported into...

journal_title：Molecular microbiology

authors： Fiori A,Francisci S,Falcone C

更新日期：2002-10-01 00:00:00