Abstract:
PURPOSE:To investigate the presence of the fibronectin isoform containing the extradomain B (B-FN), a marker-protein of angiogenesis, in surgically excised human choroidal neovascular membranes (CNVM) to evaluate whether B-FN could be used as a therapeutic target for specific antibody-photosensitizer immunoconjugates. DESIGN:Laboratory investigation. METHODS:The setting was an institutional practice. The study population consisted of 15 eyes (15 patients) with CNVM undergoing membrane excision (four eyes with age-related macular degeneration, seven with pathologic myopia and four with multifocal choroiditis). The control group consisted of eight eye bank eyes (four subjects) without choroidal neovascularization. Light microscopic immunohistochemistry on cryostat sections of tissues was obtained. B-FN was detected by a human recombinant antibody, CGS-1, and compared with immunostaining for endothelial cells with factor VIII-related antigen. The main outcome measure was the presence of CGS-1 positively stained cells or areas of the extracellular matrix. Staining of CGS-1 was scored on a scale from 0 to 3. RESULTS:Fourteen of 15 neovascular membranes stained strongly with CGS-1 (score 2 or 3). One membrane from a patient with pathologic myopia was negatively stained (score 0). CGS-1 positive staining was detected around endothelial cells and in the extracellular matrix of CNVMs. The retina of eyes without choroidal neovascularization was negative with CGS-1 in all eight donor eyes, while the choroid contained some weakly CGS-1 positive cells (score 0 and 1, respectively). CONCLUSIONS:The extradomain B is abundantly expressed in CNVMs, but its expression is more restricted in eyes harboring no apparent choroidal neovascularization. In the future, B-FN might serve as a target for the delivery of antibody-photosensitizer immunoconjugates to newly developed vessels to enhance the selectivity of photodynamic therapy.
journal_name
Am J Ophthalmoljournal_title
American journal of ophthalmologyauthors
Nicolò M,Birò A,Cardillo-Piccolino F,Castellani P,Giovannini A,Mariotti C,Zingirian M,Neri D,Zardi Ldoi
10.1016/s0002-9394(02)01839-1subject
Has Abstractpub_date
2003-01-01 00:00:00pages
7-13issue
1eissn
0002-9394issn
1879-1891pii
S0002939402018391journal_volume
135pub_type
杂志文章abstract::We analyzed 34 sets of optic disk photographs from 19 patients with glaucoma who had been followed up for ten years to evaluate changes in the position of the vasculature of the optic disks over time. Each photograph was analyzed by an image analyzer's edge-defining program for optic disk analysis and by three experie...
journal_title:American journal of ophthalmology
pub_type: 杂志文章
doi:10.1016/s0002-9394(14)74101-7
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abstract::Two homosexual men, 35 and 42 years old, had bilateral acute angle-closure glaucoma in association with the acquired immune deficiency syndrome. In one patient, the angle-closure attack was the initial manifestation of AIDS. Choroidal effusion with secondary anterior rotation of the ciliary body at the scleral spur ap...
journal_title:American journal of ophthalmology
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journal_title:American journal of ophthalmology
pub_type: 临床试验,杂志文章,随机对照试验
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journal_title:American journal of ophthalmology
pub_type: 杂志文章,多中心研究
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更新日期:2008-06-01 00:00:00
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更新日期:1994-03-15 00:00:00
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journal_title:American journal of ophthalmology
pub_type: 杂志文章
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更新日期:2004-07-01 00:00:00
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journal_title:American journal of ophthalmology
pub_type: 临床试验,杂志文章
doi:
更新日期:1981-10-01 00:00:00
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更新日期:2007-04-01 00:00:00
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journal_title:American journal of ophthalmology
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journal_title:American journal of ophthalmology
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更新日期:2002-11-01 00:00:00
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journal_title:American journal of ophthalmology
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journal_title:American journal of ophthalmology
pub_type: 杂志文章
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更新日期:2006-06-01 00:00:00
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journal_title:American journal of ophthalmology
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journal_title:American journal of ophthalmology
pub_type: 杂志文章
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更新日期:2007-02-01 00:00:00
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journal_title:American journal of ophthalmology
pub_type: 杂志文章
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更新日期:1993-01-01 00:00:00