Analysis of gene expression and insulin secretion by monolayer-forming adult porcine pancreatic endocrine cells.

Abstract:

INTRODUCTION:We recently established a method of isolation and primary monolayer culture of porcine pancreatic endocrine cells that involves the use of nicotinamide. AIM:To obtain genetic information on cultured porcine endocrine cells and to examine cell function in relation to insulin secretion during long-term culture. METHODOLOGY:Gene expression of insulin and several transcription factors, including PDX-1, Beta2/NeuroD, Pax6, and Nkx6.1, was assessed by reverse transcription-polymerase chain reaction analysis, and the insulin protein level was estimated by immunohistochemistry and enzyme assay during a 12-week period. RESULTS:During the culture period, insulin accumulation in the medium at 5 weeks had decreased by almost half the level of accumulation in the first week. In contrast to the alteration of secretory function, insulin gene expression was maintained for at least 12 weeks, and regulatory transcription factors were expressed at the same levels until 9 weeks. These observations suggest that gene expression is not involved in the cause of decreased baseline insulin secretion. Moreover, although the insulin response to high glucose and potassium loading was maintained, the magnitude of the responses to both stimuli was attenuated in the late period of culture. Insulin secretion tended to decrease in our culture system, and the secretory response to pharmacological stimulation was attenuated despite maintenance of messenger RNA expression of insulin and other islet-specific genes for at least 9 weeks in vitro. CONCLUSION:These findings indicate that cell integrity is maintained and that the alteration in insulin secretion must be explained by another mechanism.

journal_name

Pancreas

journal_title

Pancreas

authors

Tsuchiya M,Tsuchiya K,Iwami Y,Ohgawara H

doi

10.1097/00006676-200301000-00012

subject

Has Abstract

pub_date

2003-01-01 00:00:00

pages

71-5

issue

1

eissn

0885-3177

issn

1536-4828

pii

00006676-200301000-00012

journal_volume

26

pub_type

杂志文章

相关文献

PANCREAS文献大全
  • Is Multifocality an Indicator of Aggressive Behavior in Small Bowel Neuroendocrine Tumors?

    abstract:OBJECTIVES:Many patients with small bowel neuroendocrine tumors (SBNETs) have multifocal tumors (MFTs), but the frequency of MFTs has varied widely across SBNET studies. It is also unclear whether patients with MFTs have more advanced disease or worse clinical course than do those with unifocal SBNETs. We set out to de...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000000911

    authors: Choi AB,Maxwell JE,Keck KJ,Bellizzi AJ,Dillon JS,OʼDorisio TM,Howe JR

    更新日期:2017-10-01 00:00:00

  • Significance of Lymph Node Metastasis in Resectable Well-differentiated Pancreatic Neuroendocrine Tumor.

    abstract:OBJECTIVES:Understanding the effect of lymph node metastasis (LNM) on prognosis in pancreatic neuroendocrine neoplasm is helpful for surgery and follow-up. In this study, we investigated the significance of LNM in well-differentiated pancreatic neuroendocrine tumors (PanNETs) according to the World Health Organization ...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000001355

    authors: Harimoto N,Hoshino K,Muranushi R,Hagiwara K,Yamanaka T,Ishii N,Tsukagoshi M,Igarashi T,Tanaka H,Watanabe A,Kubo N,Araki K,Hosouchi Y,Suzuki H,Arakawa K,Hirai K,Fukazawa T,Ikota H,Shirabe K

    更新日期:2019-08-01 00:00:00

  • The risk of pancreaticointestinal anastomosis can be predicted preoperatively.

    abstract::The risk of developing postoperative complications following pancreatic resection depends mainly on how difficult it is to perform a proper pancreaticointestinal anastomosis. We have evaluated the serum pancreolauryl test, a rapid tubeless pancreatic function test, as a simple preoperative predictor of the degree of p...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:

    authors: Friess H,Malfertheiner P,Isenmann R,Kühne H,Beger HG,Büchler MW

    更新日期:1996-08-01 00:00:00

  • Effect of a new synthetic protease inhibitor on beta-endorphin release during acute pancreatitis in dogs.

    abstract::The effect on endogenous beta-endorphins of a new synthetic protease inhibitor was studied in acute pancreatitis. Pancreatitis was induced by the injection of autologous bile mixed with trypsin into the main pancreatic duct after ligation of the accessory duct. Plasma beta-endorphin concentrations and cardiovascular f...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199107000-00011

    authors: Satake K,Hiura A,Ha SS,Nishiwaki H,Umeyama K

    更新日期:1991-07-01 00:00:00

  • Interaction of Serum microRNAs and Serum Folate With the Susceptibility to Pancreatic Cancer.

    abstract:OBJECTIVES:The aim of this study was to investigate whether 6 candidate serum miRNAs and their interactions with serum folate level were associated with the risk for pancreatic cancer (PC). METHOD:A hospital-based case-control study including 74 incident PC cases and 74 controls was conducted. Serum folate and miRNAs ...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000000204

    authors: Tian Y,Xue Y,Ruan G,Cheng K,Tian J,Qiu Q,Xiao M,Li H,Yang H,Wang L

    更新日期:2015-01-01 00:00:00

  • Increased expression of glutathione S-transferase-pi in the islets of patients with primary chronic pancreatitis but not secondary chronic pancreatitis.

    abstract::The mechanism of tissue alteration in chronic pancreatitis (CP) is still unclear. Different hypotheses have been discussed, including increasing oxidant stress in the acinar cells, often as a result of exposure to xenobiotics. To evaluate the role of oxidative stress in CP, the authors investigated the expression of t...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-200105000-00009

    authors: Ulrich AB,Schmied BM,Matsuzaki H,Lawson TA,Friess H,Andrén-Sandberg A,Büchler MW,Pour PM

    更新日期:2001-05-01 00:00:00

  • Three female familial cases of solid pseudopapillary tumors with a protease serine 1 gene mutation.

    abstract::Solid pseudopapillary tumors (SPTs) are a rare pancreatic neoplastic lesion. Familial aggregation has not been reported in this disease. The objectives of this study were to report the history, clinicopathological features, and gene mutations of 3 familial cases of SPT. Three female cases of SPT presented in 1 family....

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0b013e3182554276

    authors: Gou S,Yu J,Wang C,Liu T,Cui P,Li X

    更新日期:2013-01-01 00:00:00

  • Pancreatic enzyme secretion mediated by novel peptide: monitor peptide hypothesis.

    abstract::A new model is proposed for pancreatic enzyme secretion in response to food protein intake in rats. We have found a novel peptide in rat bile-pancreatic juice, which exhibits a trypsin-sensitive, cholecystokinin (CCK)-releasing activity. The amino acid sequence of the peptide purified from rat bile-pancreatic juice is...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-198812000-00013

    authors: Iwai K,Fushiki T,Fukuoka S

    更新日期:1988-01-01 00:00:00

  • Development and Diagnostic Accuracy of a Breath Test for Pancreatic Exocrine Insufficiency in Chronic Pancreatitis.

    abstract:OBJECTIVE:To develop a ¹³C-labeled substrate breath test by defining the optimal ¹³C-labeled substrate, substrate dose, test meal, and duration of the test and evaluating its accuracy for the diagnosis of pancreatic exocrine insufficiency (PEI) in chronic pancreatitis (CP). METHODS:Five consecutive prospective compara...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000000434

    authors: Domínguez-Muñoz JE,Nieto L,Vilariño M,Lourido MV,Iglesias-García J

    更新日期:2016-02-01 00:00:00

  • An enzyme immunoassay for the determination of lipase in human duodenal fluid.

    abstract::The application of a commercially available enzyme immunoassay method for the determination of lipase concentration in human duodenal fluid is reported. Variations in the test procedure have been investigated. A sample incubation and a conjugate incubation of 1 h at 37 degrees C are necessary. There is substantial agr...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-198902000-00014

    authors: Hafkenscheid JC,Hessels M,Kerstens PJ,Lamers CB

    更新日期:1989-01-01 00:00:00

  • Personalized Pancreatic Cancer Management: A Systematic Review of How Machine Learning Is Supporting Decision-making.

    abstract::This review critically analyzes how machine learning is being used to support clinical decision-making in the management of potentially resectable pancreatic cancer. Following PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) guidelines, electronic searches of MEDLINE, Embase, PubMed, and Coc...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000001312

    authors: Bradley A,van der Meer R,McKay C

    更新日期:2019-05-01 00:00:00

  • Prior History of Pancreatitis Accelerates the Development of Pancreatic Adenocarcinoma.

    abstract:OBJECTIVES:Presentation of pancreatic adenocarcinoma (PC) as acute pancreatitis (AP), association of chronic pancreatitis (CP) with PC, and role of inflammation in PC carcinogenesis are well recognized. We hypothesized that inflammatory changes associated with remote history of AP (≥2 years before PC diagnosis) would r...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000001160

    authors: Phillips AE,Shah N,Borhani AA,Yadav D,Brand RE

    更新日期:2018-11-01 00:00:00

  • Pancreatic acinar cells in adult human islets of Langerhans.

    abstract::Pancreatic acinar cells within the islets of Langerhans in human pancreas were investigated both histopathologically and electron microscopically. Acinar cells inside the islets of Langerhans were observed in all 50 adult cases investigated. Between such acinar cells and endocrine tissues, no reticular fiber was obser...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199409000-00004

    authors: Suda K,Hosokawa Y,Kuroda J,Yuminamochi T,Ishii Y,Nakazawa K

    更新日期:1994-09-01 00:00:00

  • Pharmacological cholinergic stimulation as a therapeutic tool in experimental necrotizing pancreatitis.

    abstract:OBJECTIVES:The endogenous immune response is influenced by the stimulation of the vagal nerve. Stimulation or ablation has a direct impact on the release of pro- and anti-inflammatory mediators. In the progression of acute pancreatitis from local to systemic disease, these mediators play a pivotal role. This study eval...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0b013e3182a85c21

    authors: Schneider L,Jabrailova B,Soliman H,Hofer S,Strobel O,Hackert T,Büchler MW,Werner J

    更新日期:2014-01-01 00:00:00

  • Immunoneutralization of circulating pancreatic polypeptide and pancreatic secretion.

    abstract::To determine the role of endogenous pancreatic polypeptide (PP) as a physiological inhibitor of pancreatic secretion, normal rabbit serum (control) or rabbit PP-antiserum was administered intravenously to dogs with chronic esophageal, gastric, and pancreatic fistulas. In all dogs tested, sham-feeding and ordinary feed...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-198709000-00008

    authors: Konturek SJ,Bielański W,Kwiecień N,Konturek J,Czarnobilski K

    更新日期:1987-01-01 00:00:00

  • Characterization of protein serine/threonine phosphatases in rat pancreas and development of an endogenous substrate-specific phosphatase assay.

    abstract::Protein phosphatases have recently been recognized to represent an important, independently regulated portion of cellular signaling cascades. Although reversible phosphorylation of multiple pancreatic proteins has been described, suggesting a role for these enzymes, little is known about the characteristics of protein...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199407000-00002

    authors: Lutz MP,Pinon DI,Miller LJ

    更新日期:1994-07-01 00:00:00

  • Effects of arginine on the secretion of insulin and islet amyloid polypeptide in humans.

    abstract::Islet amyloid polypeptide (IAPP) is synthesized in islet B cells and stored in the secretory granules. We examined whether IAPP and insulin are released in parallel in humans. Arginine hydrochloride (5 g) was injected intravenously at three glucose levels in 11 healthy 58-year-old female subjects. In the fasting state...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199508000-00015

    authors: Larsson H,Ahrén B

    更新日期:1995-08-01 00:00:00

  • Amylase level in drains after pancreatoduodenectomy as a predictor of clinically significant pancreatic fistula.

    abstract:OBJECTIVES:Amylase level in drains (ALD) has been proposed as a predictor for the development of a clinically significant pancreatic fistula (CS-PF) in patients undergoing a major pancreatic surgery. This study aimed to determine if the ALD in patients who developed a CS-PF after pancreatoduodenectomy is higher than th...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000000060

    authors: Ceroni M,Galindo J,Guerra JF,Salinas J,Martínez J,Jarufe N

    更新日期:2014-04-01 00:00:00

  • c-met expression in pancreatic cancer and effects of hepatocyte growth factor on pancreatic cancer cell growth.

    abstract::Hepatocyte growth factor (HGF) is a widely expressed growth factor secreted by cells of mesenchymal origin, which has been shown to be involved in growth processes of multiple cell types. The HGF receptor, the product of the c-met protooncogene, is expressed mainly by epithelial cells. Increased expression of the HGF ...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199707000-00005

    authors: Kiehne K,Herzig KH,Fölsch UR

    更新日期:1997-07-01 00:00:00

  • A population-based evaluation of severity and mortality among transferred patients with acute pancreatitis.

    abstract:OBJECTIVES:This study aimed to compare severity of acute pancreatitis (AP) and mortality rates between transferred and nontransferred patients and to determine the factors that influence the decision to transfer. METHODS:A retrospective analysis coding a statewide administrative database in Maryland was conducted. Sev...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000000179

    authors: Anand G,Hutfless SM,Akshintala VS,Khashab MA,Lennon AM,Makary MA,Hirose K,Andersen DK,Kalloo AN,Singh VK

    更新日期:2014-10-01 00:00:00

  • Stimulatory and inhibitory effects of galanin on exocrine and endocrine rat pancreas.

    abstract::The influence of the neuropeptide galanin, present in intrapancreatic nerve endings, on the endocrine pancreas is well known. The most potent effect of galanin is inhibition of insulin release. Little is known of its effect on the exocrine pancreas. Whether galanin plays a role in the regulation of exocrine pancreatic...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199211000-00001

    authors: Rünzi M,Müller MK,Schmid P,von Schönfeld J,Goebell H

    更新日期:1992-01-01 00:00:00

  • Expression of the beacon gene in the rat pancreatic islets: opposite effects of beacon (47-73) protein (ubiquitin-like protein 5) on insulin secretion in vivo and insulin release by isolated islets.

    abstract:OBJECTIVE:Beacon gene expression is elevated in the hypothalamus of the Israeli sand rat (Psammomys obesus), an animal that is used as a polygenic animal model of obesity and NIDDM. We performed studies aimed at investigating the expression of beacon mRNA and protein in pancreatic islets of the rat and the possible bea...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-200408000-00003

    authors: Nowak KW,Ruciński M,Kaczmarek P,Szkudelski T,Malendowicz LK

    更新日期:2004-08-01 00:00:00

  • Tumor angiogenesis in chronic pancreatitis and pancreatic adenocarcinoma: impact of K-ras mutations.

    abstract::Chronic pancreatitis (CP) is one condition in which epidemiologic studies have demonstrated a definite association with pancreatic adenocarcinoma (PAC). The pathophysiologic and molecular events that either predispose to the development of, or potentiate the growth of, PAC are unknown. Mutation of the codon 12 K-ras g...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-200004000-00005

    authors: Banerjee SK,Zoubine MN,Mullick M,Weston AP,Cherian R,Campbell DR

    更新日期:2000-04-01 00:00:00

  • Altered expression of insulin-like growth factor II receptor in human pancreatic cancer.

    abstract::The insulin-like growth factor-II (IGF-II) receptor (IGF-IIR) is a single-chain transmembrane protein identical to the mannose-6-phosphate receptor. In the present study we examined IGF-IIR expression in normal and cancerous human pancreatic tissues. In the normal pancreas, moderately strong IGF-IIR immunoreactivity w...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199711000-00006

    authors: Ishiwata T,Bergmann U,Kornmann M,Lopez M,Beger HG,Korc M

    更新日期:1997-11-01 00:00:00

  • Diagnostic Performance of 48-Hour Fasting Test and Insulin Surrogates in Patients With Suspected Insulinoma.

    abstract:OBJECTIVES:This study aimed to evaluate the usefulness of the 48-hour fasting test and insulin surrogates followed by a glucagon stimulatory test (GST) for the diagnosis of insulinoma. METHODS:Thirty-five patients with suspected insulinoma who underwent 48-hour fasting test and GST were retrospectively included in our...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000000772

    authors: Ueda K,Kawabe K,Lee L,Tachibana Y,Fujimori N,Igarashi H,Oda Y,Jensen RT,Takayanagi R,Ito T

    更新日期:2017-04-01 00:00:00

  • The Research of Acellular Pancreatic Bioscaffold as a Natural 3-Dimensional Platform In Vitro.

    abstract:OBJECTIVE:The aim of the study was to investigate the biochemical and functional properties of a rat acellular pancreatic bioscaffolds (APBs). METHODS:Fresh pancreata from 10 rats were soaked and perfused through portal veins using Easy-Load Digital Drive peristaltic pumps. The histological structure, extracellular ma...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000001123

    authors: Wang X,Li YG,Du Y,Zhu JY,Li Z

    更新日期:2018-09-01 00:00:00

  • Biochemical and histochemical characterization of cultured rat and hamster pancreatic ducts.

    abstract::Pancreatic duct fragments were isolated from rat and hamster pancreas and were cultured in an agarose matrix for up to 8 weeks (rat) or 20 weeks (hamster). The fragments consisted predominantly of duct epithelium, lesser numbers of stromal and atrophied acinar cells, and small numbers of islet cells. Hamster ducts ave...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-198707000-00010

    authors: Githens S,Finley JJ,Patke CL,Schexnayder JA,Fallon KB,Ruby JR

    更新日期:1987-01-01 00:00:00

  • Synthesis of steroids in pancreas: evidence of cytochrome P-450scc activity.

    abstract::In pancreas, the activities of several sex steroid-transforming enzymes have been reported. Data have been obtained in perfused organs, total tissue homogenates, and subcellular organelles. These data, concurrent with the description of the presence of ligand-regulated steroid receptors, as well as the sexually dimorp...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-199907000-00006

    authors: Morales A,Cuéllar A,Ramírez J,Vilchis F,Díaz-Sánchez V

    更新日期:1999-07-01 00:00:00

  • Cytoreductive Surgery for Pancreatic Cancer Improves Overall Outcome of Gemcitabine-Based Chemotherapy.

    abstract:OBJECTIVES:Pancreatoduodenectomy is feasible also in patients with locally advanced pancreatic adenocarcinoma (PA) nowadays. Data on risk and survival analysis of palliative pancreatic resections followed by gemcitabine-based chemotherapy (Cx) are limited. METHODS:Between 2000 and 2009, a total of 45 patients had prim...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/MPA.0000000000000365

    authors: Bahra M,Pratschke J,Klein F,Neuhaus P,Boas-Knoop S,Puhl G,Denecke T,Pullankavumkal JR,Sinn M,Riess H,Pelzer U

    更新日期:2015-08-01 00:00:00

  • Troglitazone stimulates pancreatic growth in normal rats.

    abstract:INTRODUCTION:Peroxisome proliferator-activated receptors (PPARs) are a family of ligand-activated nuclear transcription factors belonging to the nuclear hormone receptors. Troglitazone, a specific ligand for PPAR-gamma is shown to regulate not only lipids and glucose metabolism, but also cell cycle, differentiation, an...

    journal_title:Pancreas

    pub_type: 杂志文章

    doi:10.1097/00006676-200204000-00015

    authors: Jia DM,Otsuki M

    更新日期:2002-04-01 00:00:00