Abstract:
INTRODUCTION:We recently established a method of isolation and primary monolayer culture of porcine pancreatic endocrine cells that involves the use of nicotinamide. AIM:To obtain genetic information on cultured porcine endocrine cells and to examine cell function in relation to insulin secretion during long-term culture. METHODOLOGY:Gene expression of insulin and several transcription factors, including PDX-1, Beta2/NeuroD, Pax6, and Nkx6.1, was assessed by reverse transcription-polymerase chain reaction analysis, and the insulin protein level was estimated by immunohistochemistry and enzyme assay during a 12-week period. RESULTS:During the culture period, insulin accumulation in the medium at 5 weeks had decreased by almost half the level of accumulation in the first week. In contrast to the alteration of secretory function, insulin gene expression was maintained for at least 12 weeks, and regulatory transcription factors were expressed at the same levels until 9 weeks. These observations suggest that gene expression is not involved in the cause of decreased baseline insulin secretion. Moreover, although the insulin response to high glucose and potassium loading was maintained, the magnitude of the responses to both stimuli was attenuated in the late period of culture. Insulin secretion tended to decrease in our culture system, and the secretory response to pharmacological stimulation was attenuated despite maintenance of messenger RNA expression of insulin and other islet-specific genes for at least 9 weeks in vitro. CONCLUSION:These findings indicate that cell integrity is maintained and that the alteration in insulin secretion must be explained by another mechanism.
journal_name
Pancreasjournal_title
Pancreasauthors
Tsuchiya M,Tsuchiya K,Iwami Y,Ohgawara Hdoi
10.1097/00006676-200301000-00012subject
Has Abstractpub_date
2003-01-01 00:00:00pages
71-5issue
1eissn
0885-3177issn
1536-4828pii
00006676-200301000-00012journal_volume
26pub_type
杂志文章相关文献
PANCREAS文献大全abstract:OBJECTIVES:Many patients with small bowel neuroendocrine tumors (SBNETs) have multifocal tumors (MFTs), but the frequency of MFTs has varied widely across SBNET studies. It is also unclear whether patients with MFTs have more advanced disease or worse clinical course than do those with unifocal SBNETs. We set out to de...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000911
更新日期:2017-10-01 00:00:00
abstract:OBJECTIVES:Understanding the effect of lymph node metastasis (LNM) on prognosis in pancreatic neuroendocrine neoplasm is helpful for surgery and follow-up. In this study, we investigated the significance of LNM in well-differentiated pancreatic neuroendocrine tumors (PanNETs) according to the World Health Organization ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000001355
更新日期:2019-08-01 00:00:00
abstract::The risk of developing postoperative complications following pancreatic resection depends mainly on how difficult it is to perform a proper pancreaticointestinal anastomosis. We have evaluated the serum pancreolauryl test, a rapid tubeless pancreatic function test, as a simple preoperative predictor of the degree of p...
journal_title:Pancreas
pub_type: 杂志文章
doi:
更新日期:1996-08-01 00:00:00
abstract::The effect on endogenous beta-endorphins of a new synthetic protease inhibitor was studied in acute pancreatitis. Pancreatitis was induced by the injection of autologous bile mixed with trypsin into the main pancreatic duct after ligation of the accessory duct. Plasma beta-endorphin concentrations and cardiovascular f...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199107000-00011
更新日期:1991-07-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to investigate whether 6 candidate serum miRNAs and their interactions with serum folate level were associated with the risk for pancreatic cancer (PC). METHOD:A hospital-based case-control study including 74 incident PC cases and 74 controls was conducted. Serum folate and miRNAs ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000204
更新日期:2015-01-01 00:00:00
abstract::The mechanism of tissue alteration in chronic pancreatitis (CP) is still unclear. Different hypotheses have been discussed, including increasing oxidant stress in the acinar cells, often as a result of exposure to xenobiotics. To evaluate the role of oxidative stress in CP, the authors investigated the expression of t...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200105000-00009
更新日期:2001-05-01 00:00:00
abstract::Solid pseudopapillary tumors (SPTs) are a rare pancreatic neoplastic lesion. Familial aggregation has not been reported in this disease. The objectives of this study were to report the history, clinicopathological features, and gene mutations of 3 familial cases of SPT. Three female cases of SPT presented in 1 family....
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3182554276
更新日期:2013-01-01 00:00:00
abstract::A new model is proposed for pancreatic enzyme secretion in response to food protein intake in rats. We have found a novel peptide in rat bile-pancreatic juice, which exhibits a trypsin-sensitive, cholecystokinin (CCK)-releasing activity. The amino acid sequence of the peptide purified from rat bile-pancreatic juice is...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-198812000-00013
更新日期:1988-01-01 00:00:00
abstract:OBJECTIVE:To develop a ¹³C-labeled substrate breath test by defining the optimal ¹³C-labeled substrate, substrate dose, test meal, and duration of the test and evaluating its accuracy for the diagnosis of pancreatic exocrine insufficiency (PEI) in chronic pancreatitis (CP). METHODS:Five consecutive prospective compara...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000434
更新日期:2016-02-01 00:00:00
abstract::The application of a commercially available enzyme immunoassay method for the determination of lipase concentration in human duodenal fluid is reported. Variations in the test procedure have been investigated. A sample incubation and a conjugate incubation of 1 h at 37 degrees C are necessary. There is substantial agr...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-198902000-00014
更新日期:1989-01-01 00:00:00
abstract::This review critically analyzes how machine learning is being used to support clinical decision-making in the management of potentially resectable pancreatic cancer. Following PRISMA (Preferred Reporting Items for Systematic Reviews and Meta-analyses) guidelines, electronic searches of MEDLINE, Embase, PubMed, and Coc...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000001312
更新日期:2019-05-01 00:00:00
abstract:OBJECTIVES:Presentation of pancreatic adenocarcinoma (PC) as acute pancreatitis (AP), association of chronic pancreatitis (CP) with PC, and role of inflammation in PC carcinogenesis are well recognized. We hypothesized that inflammatory changes associated with remote history of AP (≥2 years before PC diagnosis) would r...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000001160
更新日期:2018-11-01 00:00:00
abstract::Pancreatic acinar cells within the islets of Langerhans in human pancreas were investigated both histopathologically and electron microscopically. Acinar cells inside the islets of Langerhans were observed in all 50 adult cases investigated. Between such acinar cells and endocrine tissues, no reticular fiber was obser...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199409000-00004
更新日期:1994-09-01 00:00:00
abstract:OBJECTIVES:The endogenous immune response is influenced by the stimulation of the vagal nerve. Stimulation or ablation has a direct impact on the release of pro- and anti-inflammatory mediators. In the progression of acute pancreatitis from local to systemic disease, these mediators play a pivotal role. This study eval...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0b013e3182a85c21
更新日期:2014-01-01 00:00:00
abstract::To determine the role of endogenous pancreatic polypeptide (PP) as a physiological inhibitor of pancreatic secretion, normal rabbit serum (control) or rabbit PP-antiserum was administered intravenously to dogs with chronic esophageal, gastric, and pancreatic fistulas. In all dogs tested, sham-feeding and ordinary feed...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-198709000-00008
更新日期:1987-01-01 00:00:00
abstract::Protein phosphatases have recently been recognized to represent an important, independently regulated portion of cellular signaling cascades. Although reversible phosphorylation of multiple pancreatic proteins has been described, suggesting a role for these enzymes, little is known about the characteristics of protein...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199407000-00002
更新日期:1994-07-01 00:00:00
abstract::Islet amyloid polypeptide (IAPP) is synthesized in islet B cells and stored in the secretory granules. We examined whether IAPP and insulin are released in parallel in humans. Arginine hydrochloride (5 g) was injected intravenously at three glucose levels in 11 healthy 58-year-old female subjects. In the fasting state...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199508000-00015
更新日期:1995-08-01 00:00:00
abstract:OBJECTIVES:Amylase level in drains (ALD) has been proposed as a predictor for the development of a clinically significant pancreatic fistula (CS-PF) in patients undergoing a major pancreatic surgery. This study aimed to determine if the ALD in patients who developed a CS-PF after pancreatoduodenectomy is higher than th...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000060
更新日期:2014-04-01 00:00:00
abstract::Hepatocyte growth factor (HGF) is a widely expressed growth factor secreted by cells of mesenchymal origin, which has been shown to be involved in growth processes of multiple cell types. The HGF receptor, the product of the c-met protooncogene, is expressed mainly by epithelial cells. Increased expression of the HGF ...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199707000-00005
更新日期:1997-07-01 00:00:00
abstract:OBJECTIVES:This study aimed to compare severity of acute pancreatitis (AP) and mortality rates between transferred and nontransferred patients and to determine the factors that influence the decision to transfer. METHODS:A retrospective analysis coding a statewide administrative database in Maryland was conducted. Sev...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000179
更新日期:2014-10-01 00:00:00
abstract::The influence of the neuropeptide galanin, present in intrapancreatic nerve endings, on the endocrine pancreas is well known. The most potent effect of galanin is inhibition of insulin release. Little is known of its effect on the exocrine pancreas. Whether galanin plays a role in the regulation of exocrine pancreatic...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199211000-00001
更新日期:1992-01-01 00:00:00
abstract:OBJECTIVE:Beacon gene expression is elevated in the hypothalamus of the Israeli sand rat (Psammomys obesus), an animal that is used as a polygenic animal model of obesity and NIDDM. We performed studies aimed at investigating the expression of beacon mRNA and protein in pancreatic islets of the rat and the possible bea...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200408000-00003
更新日期:2004-08-01 00:00:00
abstract::Chronic pancreatitis (CP) is one condition in which epidemiologic studies have demonstrated a definite association with pancreatic adenocarcinoma (PAC). The pathophysiologic and molecular events that either predispose to the development of, or potentiate the growth of, PAC are unknown. Mutation of the codon 12 K-ras g...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200004000-00005
更新日期:2000-04-01 00:00:00
abstract::The insulin-like growth factor-II (IGF-II) receptor (IGF-IIR) is a single-chain transmembrane protein identical to the mannose-6-phosphate receptor. In the present study we examined IGF-IIR expression in normal and cancerous human pancreatic tissues. In the normal pancreas, moderately strong IGF-IIR immunoreactivity w...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199711000-00006
更新日期:1997-11-01 00:00:00
abstract:OBJECTIVES:This study aimed to evaluate the usefulness of the 48-hour fasting test and insulin surrogates followed by a glucagon stimulatory test (GST) for the diagnosis of insulinoma. METHODS:Thirty-five patients with suspected insulinoma who underwent 48-hour fasting test and GST were retrospectively included in our...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000772
更新日期:2017-04-01 00:00:00
abstract:OBJECTIVE:The aim of the study was to investigate the biochemical and functional properties of a rat acellular pancreatic bioscaffolds (APBs). METHODS:Fresh pancreata from 10 rats were soaked and perfused through portal veins using Easy-Load Digital Drive peristaltic pumps. The histological structure, extracellular ma...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000001123
更新日期:2018-09-01 00:00:00
abstract::Pancreatic duct fragments were isolated from rat and hamster pancreas and were cultured in an agarose matrix for up to 8 weeks (rat) or 20 weeks (hamster). The fragments consisted predominantly of duct epithelium, lesser numbers of stromal and atrophied acinar cells, and small numbers of islet cells. Hamster ducts ave...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-198707000-00010
更新日期:1987-01-01 00:00:00
abstract::In pancreas, the activities of several sex steroid-transforming enzymes have been reported. Data have been obtained in perfused organs, total tissue homogenates, and subcellular organelles. These data, concurrent with the description of the presence of ligand-regulated steroid receptors, as well as the sexually dimorp...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-199907000-00006
更新日期:1999-07-01 00:00:00
abstract:OBJECTIVES:Pancreatoduodenectomy is feasible also in patients with locally advanced pancreatic adenocarcinoma (PA) nowadays. Data on risk and survival analysis of palliative pancreatic resections followed by gemcitabine-based chemotherapy (Cx) are limited. METHODS:Between 2000 and 2009, a total of 45 patients had prim...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/MPA.0000000000000365
更新日期:2015-08-01 00:00:00
abstract:INTRODUCTION:Peroxisome proliferator-activated receptors (PPARs) are a family of ligand-activated nuclear transcription factors belonging to the nuclear hormone receptors. Troglitazone, a specific ligand for PPAR-gamma is shown to regulate not only lipids and glucose metabolism, but also cell cycle, differentiation, an...
journal_title:Pancreas
pub_type: 杂志文章
doi:10.1097/00006676-200204000-00015
更新日期:2002-04-01 00:00:00