Improved method for measurement of inorganic phosphate in serum with a centrifugal analyzer.

Abstract:

:A direct mehtod [Clin. Chim. Acta 46, 113 (1973)] for determination of inorganic phosphate in serum was adapted for use with a centrifugal analyzer. Contamination is minimized and analysis rate maximized by doing the reaction in the reagent wells of the transfer disc and by utilizing the high-speed spectrophotometric and data-reduction capabilities of the centrifugal analyzer. Hemolysis, icterus, and moderate lipemia cause no interference. Grossly lipemic sera and sera from patients with plasma cell dyscrasias can be analyzed by incorporating appropriate blanking and dilution techniques. The method exhibits excellent sensitivity and precision and results correlate well with those from a continuous-flow procedure.

journal_name

Clin Chem

journal_title

Clinical chemistry

authors

Wentz PW,Savory J,Cross RE

subject

Has Abstract

pub_date

1976-02-01 00:00:00

pages

257-60

issue

2

eissn

0009-9147

issn

1530-8561

journal_volume

22

pub_type

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