Abstract:
:The mouse knockout of the estrogen receptor alpha (ERalpha) gene, known as alphaERKO, has been extensively used for several years to study the role and function of ERalpha. Residual estradiol binding capacity in uterine tissue of 5-10% raised doubts if this knockout is a genuine null mutation of ERalpha. Although alternatively spliced ERalpha mRNA variants in the alphaERKO mouse were reported previously, the corresponding protein isoforms have not been detected to date. Here we show that a variant ERalpha protein, 61 kDa in size, is expressed in the uterine tissue of alphaERKO mice as a result of an alternative splicing. The transactivation capability of this protein is cell dependent and can be as high as 75% of the wild type ERalpha.
journal_name
J Mol Endocrinoljournal_title
Journal of molecular endocrinologyauthors
Kos M,Denger S,Reid G,Korach KS,Gannon Fdoi
10.1677/jme.0.0290281subject
Has Abstractpub_date
2002-12-01 00:00:00pages
281-6issue
3eissn
0952-5041issn
1479-6813journal_volume
29pub_type
杂志文章abstract::cDNA clones coding for rabbit prolactin were isolated from a pituitary library using a rat prolactin RNA probe. One cDNA contained 873 bases including the entire coding sequence of rabbit prolactin, its signal peptide and the 5' and 3' untranslated regions of 44 and 145 nucleotides respectively. The deduced amino acid...
journal_title:Journal of molecular endocrinology
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