Synthetic ceramides induce growth arrest or apoptosis by altering cellular redox status.

Abstract:

:Reactive oxygen species (ROS) and ceramide are each partly responsible for the signal transduction of a variety of extracellular agents. Furthermore, the application of synthetic, short-chain ceramides mimics the cellular responses to these extracellular agents. However, the significance of ROS involvement in ceramide signaling pathways is poorly understood. Here we describe that the cellular responses to C2-/C6-ceramide of growth arrest in U937 monocytes and apoptosis in Jurkat T-cells are preceded by a rise in mitochondrial peroxide production. In Jurkat T-cells, this is associated with a large time- and dose-dependent loss of cellular glutathione. However, in U937 monocytes, glutathione loss is transient. Differences in the magnitude and kinetics of this alteration in cellular redox state associate with discrete outcomes, namely growth arrest or apoptosis.

journal_name

Arch Biochem Biophys

authors

Phillips DC,Allen K,Griffiths HR

doi

10.1016/s0003-9861(02)00496-4

subject

Has Abstract

pub_date

2002-11-01 00:00:00

pages

15-24

issue

1

eissn

0003-9861

issn

1096-0384

pii

S0003986102004964

journal_volume

407

pub_type

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