Suppression of a nuclear frameshift mutation by a mitochondrial tRNA in the yeast Kluyveromyces lactis.

Abstract:

:A fragment of mitochondrial DNA containing the Kluyveromyces lactis gene for valine-tRNA (tRNAVAL) was isolated as a multicopy suppressor of a respiratory-deficient mutant of this yeast. The mutant produced a truncated Cox14p because of a +1 frameshift mutation in COX14, a nuclear gene encoding a protein imported into mitochondria which is necessary for respiration (Fiori et al. 2000 Yeast 16: 307-314). We report here that the mitochondrial tRNAVAL gene, when transformed into K. lactis cells, is transcribed outside mitochondria and suppresses the frameshift mutation in COX14 restoring the correct reading frame during translation of its mRNA. In fact, using histidine tagging, the existence of a suppressed Cox14p of normal length was demonstrated in mutants expressing the mt-tRNAVAL from the nucleus. Suppression could occur through a non-canonical four base pairing between the tRNAVAL and the mutated mRNA or through slippage of ribosomes during translation. This is a new case of informational suppression in that the suppression of a chromosomal mutation is not caused by a second mutation but to a mislocalization/expression of a mt-tRNA.

journal_name

Mol Microbiol

journal_title

Molecular microbiology

authors

Fiori A,Francisci S,Falcone C

doi

10.1046/j.1365-2958.2002.03147.x

subject

Has Abstract

pub_date

2002-10-01 00:00:00

pages

169-77

issue

1

eissn

0950-382X

issn

1365-2958

pii

3147

journal_volume

46

pub_type

杂志文章
  • Regulation of the CYB2 gene expression: transcriptional co-ordination by the Hap1p, Hap2/3/4/5p and Adr1p transcription factors.

    abstract::Expression of the Saccharomyces cerevisiae nuclear gene CYB2 encoding the mitochondrial enzyme L-(+)-lactate-cytochrome c oxidoreductase (EC 1.2.2.3) is subject to several strict metabolic controls at the transcriptional level: repression due to glucose fermentation, derepression by ethanol, induction by lactate and i...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2000.02065.x

    authors: Ramil E,Agrimonti C,Shechter E,Gervais M,Guiard B

    更新日期:2000-09-01 00:00:00

  • Heterologous expression and self-assembly of the S-layer protein SbsA of Bacillus stearothermophilus in Escherichia coli.

    abstract::The cell surface of Bacillus stearothermophilus PV72 is covered by a regular surface layer (S-layer) composed of single species of protein, SbsA, with a molecular weight of 130,000. Recently, the sequence of the corresponding gene (sbsA) has been determined. The SbsA coding region including the signal sequence was clo...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1996.386918.x

    authors: Kuen B,Sára M,Lubitz W

    更新日期:1996-02-01 00:00:00

  • Magnesium depletion triggers production of an immune modulating diterpenoid in Mycobacterium tuberculosis.

    abstract::Mycobacterium tuberculosis (Mtb) is the causative agent of the human disease Tuberculosis, and remains a worldwide health threat responsible for ∼1.7 million deaths annually. During infection, Mtb prevents acidification of the engulfing phagosome, thus blocking endocytic progression and eventually leading to stable re...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2011.07545.x

    authors: Mann FM,VanderVen BC,Peters RJ

    更新日期:2011-03-01 00:00:00

  • Characterization of the diphtheria toxin receptor-binding domain.

    abstract::The carboxyl-terminal region of diphtheria toxin (DT) has been analysed in order to determine regions of receptor recognition. Biochemical cleavage of the toxin with hydroxylamine (HA) was used to generate the peptides HA9DT (residues 454-535), HA6DT (residues 482-535), and HA3DT (residues 454-481). Characterization o...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1993.tb01149.x

    authors: Rolf JM,Eidels L

    更新日期:1993-02-01 00:00:00

  • Phosphatases modulate the bistable sporulation gene expression pattern in Bacillus subtilis.

    abstract::Summary Spore formation in the Gram-positive bacterium Bacillus subtilis is a last resort adaptive response to starvation. To initiate sporulation, the key regulator in this process, Spo0A, needs to be activated by the so-called phosphorelay. Within a sporulating culture of B. subtilis, some cells initiate this develo...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2005.04659.x

    authors: Veening JW,Hamoen LW,Kuipers OP

    更新日期:2005-06-01 00:00:00

  • Cloning and sequence analysis of linear plasmid telomeres of the bacterium Borrelia burgdorferi.

    abstract::Borrelia burgdorferi, the Lyme disease agent, has double-stranded linear plasmids with covalently closed ends. DNA at the ends, or telomeres, of two linear plasmids of B. burgdorferi strain B31 was examined. Telomeric sequences from both ends of a 16 kb linear plasmid and from one end of a 49 kb linear plasmid were cl...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1990.tb00651.x

    authors: Hinnebusch J,Bergström S,Barbour AG

    更新日期:1990-05-01 00:00:00

  • Eeyarestatin 24 impairs SecYEG-dependent protein trafficking and inhibits growth of clinically relevant pathogens.

    abstract::Eeyarestatin 1 (ES1) is an inhibitor of endoplasmic reticulum (ER) associated protein degradation, Sec61-dependent Ca2+ homeostasis and protein translocation into the ER. Recently, evidence was presented showing that a smaller analog of ES1, ES24, targets the Sec61-translocon, and captures it in an open conformation t...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.14589

    authors: Steenhuis M,Koningstein GM,Oswald J,Pick T,O'Keefe S,Koch HG,Cavalié A,Whitehead RC,Swanton E,High S,Luirink J

    更新日期:2020-08-15 00:00:00

  • Pkn9, a Ser/Thr protein kinase involved in the development of Myxococcus xanthus.

    abstract::The Myxococcus xanthus gene, pkn9, encodes a protein that contains significant homology with eukaryotic Ser/Thr protein kinases. The pkn9 gene was singled out of a previously identified family of kinase genes by amplification techniques that displayed differences in kinase gene expression during selected periods of th...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1997.d01-1871.x

    authors: Hanlon WA,Inouye M,Inouye S

    更新日期:1997-02-01 00:00:00

  • The periplasmic domains of Vibrio cholerae ToxR and ToxS are forming a strong heterodimeric complex independent on the redox state of ToxR cysteines.

    abstract::The transmembrane protein ToxR plays a key role in the virulence expression system of Vibrio cholerae. The activity of ToxR is dependent on its periplasmic sensor domain (ToxRp) and on the inner membrane protein ToxS. Herein, we present the Nuclear Magnetic Resonance NMR solution structure of the sensory ToxRp contain...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.14673

    authors: Gubensäk N,Wagner GE,Schrank E,Falsone FS,Berger TMI,Pavkov-Keller T,Reidl J,Zangger K

    更新日期:2020-12-23 00:00:00

  • Identifying a novel connection between the fungal plasma membrane and pH-sensing.

    abstract::The mechanisms by which micro-organisms sense and internalize extracellular pH signals are not completely understood. One example of a known external pH-sensing process is the fungal-specific Rim/Pal signal transduction pathway. Fungi, such as the opportunistic pathogen Cryptococcus neoformans, use Rim signaling to se...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.13998

    authors: Brown HE,Ost KS,Esher SK,Pianalto KM,Saelens JW,Guan Z,Andrew Alspaugh J

    更新日期:2018-08-01 00:00:00

  • Novel coordination of lipopolysaccharide modifications in Vibrio cholerae promotes CAMP resistance.

    abstract::In the environment and during infection, the human intestinal pathogen Vibrio cholerae must overcome noxious compounds that damage the bacterial outer membrane. The El Tor and classical biotypes of O1 V. cholerae show striking differences in their resistance to membrane disrupting cationic antimicrobial peptides (CAMP...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.13835

    authors: Herrera CM,Henderson JC,Crofts AA,Trent MS

    更新日期:2017-11-01 00:00:00

  • RNase E autoregulates its synthesis in Escherichia coli by binding directly to a stem-loop in the rne 5' untranslated region.

    abstract::RNase E autoregulates its production in Escherichia coli by governing the decay rate of rne (RNase E) mRNA. It does so by a mechanism that is dependent in part on hp2, a cis-acting stem-loop within the rne 5' untranslated region. In principle, hp2 could function either as a cleavage site for RNase E or as a binding si...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2009.06662.x

    authors: Schuck A,Diwa A,Belasco JG

    更新日期:2009-04-01 00:00:00

  • HobA--a novel protein involved in initiation of chromosomal replication in Helicobacter pylori.

    abstract::Replication of the bacterial chromosome is initiated by the binding of the DnaA protein to a unique DNA region, called oriC. Many regulatory factors in numerous species act by controlling the ability of DnaA to bind and unwind DNA, but the Helicobacter pylori genome does not contain homologues to any of these factors....

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2007.05853.x

    authors: Zawilak-Pawlik A,Kois A,Stingl K,Boneca IG,Skrobuk P,Piotr J,Lurz R,Zakrzewska-Czerwińska J,Labigne A

    更新日期:2007-08-01 00:00:00

  • Supercoiling of an excised genomic island represses effector gene expression to prevent activation of host resistance.

    abstract::The plant pathogen Pseudomonas syringae pv. phaseolicola, which causes halo blight disease of beans, contains a 106 kb genomic island PPHGI-1. PPHGI-1 carries a gene, avrPphB, which encodes an effector protein that triggers a resistance response in certain bean cultivars. Previous studies have shown that when PPHGI-1 ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.14111

    authors: Neale HC,Jackson RW,Preston GM,Arnold DL

    更新日期:2018-11-01 00:00:00

  • Back to log phase: sigma S as a global regulator in the osmotic control of gene expression in Escherichia coli.

    abstract::It is now well established that the sigma S subunit of RNA polymerase is a master regulator in a complex regulatory network that governs the expression of many stationary-phase-inducible genes in Escherichia coli. In this review, more recent findings will be summarized that demonstrate that sigma S also acts as a glob...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1046/j.1365-2958.1996.511405.x

    authors: Hengge-Aronis R

    更新日期:1996-09-01 00:00:00

  • The frequency of expression of pyelonephritis-associated pili is under regulatory control.

    abstract::The Escherichia coli urinary tract isolate C1212 contains two pyelonephritis-associated pili (pap) DNA sequences designated here as pap-17 and pap-21. Each of these pap sequences encodes antigenically-distinct pilin monomers, pilin-17 and pilin-21, respectively. Most individual strain C1212 cells isolated from a singl...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1987.tb01940.x

    authors: Low D,Robinson EN Jr,McGee ZA,Falkow S

    更新日期:1987-11-01 00:00:00

  • Cell-surface-associated polypeptides CshA and CshB of high molecular mass are colonization determinants in the oral bacterium Streptococcus gordonii.

    abstract::The human oral bacterium Streptococcus gordonii expresses, on the cell surface, two antigenically related high-molecular-mass polypeptides denoted CshA and CshB, encoded by genes at separate chromosomal loci. The precursor form of CshA is composed of four distinct segments: (i) a 41-amino-acid residue leader peptide, ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1994.tb01311.x

    authors: McNab R,Jenkinson HF,Loach DM,Tannock GW

    更新日期:1994-11-01 00:00:00

  • Interplay of transcriptional and small RNA-dependent control mechanisms regulates chitosugar uptake in Escherichia coli and Salmonella.

    abstract::Escherichia coli and Salmonella can use chitin-derived oligosaccharides as carbon and nitrogen sources. Chitosugars traverse the outer membrane through a dedicated chitoporin, ChiP, and are transported across the cytoplasmic membrane by the chitobiose transporter (ChbBCA). Previous work revealed that synthesis of the ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12573

    authors: Plumbridge J,Bossi L,Oberto J,Wade JT,Figueroa-Bossi N

    更新日期:2014-05-01 00:00:00

  • Sequence, exon-intron organization, transcription and mutational analysis of prnA, the gene encoding the transcriptional activator of the prn gene cluster in Aspergillus nidulans.

    abstract::The prnA gene codes for a transcriptional activator that mediates proline induction of four other genes involved in proline utilization as a nitrogen and/or carbon source in Aspergillus nidulans. In this paper, we present the genomic and cDNA sequence and the transcript map of prnA. The PrnA protein belongs to the Zn ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1998.00801.x

    authors: Cazelle B,Pokorska A,Hull E,Green PM,Stanway G,Scazzocchio C

    更新日期:1998-04-01 00:00:00

  • TUP1 disruption in Cryptococcus neoformans uncovers a peptide-mediated density-dependent growth phenomenon that mimics quorum sensing.

    abstract::Cryptococcus neoformans is a pathogenic yeast that causes life-threatening meningoencephalitis and grows well on mycological media regardless of inoculum size. Interestingly, a deletion of the global repressor TUP1 in C. neoformans uncovered a density-dependent growth phenotype reminiscent of the quorum-sensing phenom...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2007.05666.x

    authors: Lee H,Chang YC,Nardone G,Kwon-Chung KJ

    更新日期:2007-05-01 00:00:00

  • Identification, cloning and sequence analysis of the Bacillus sphaericus 1593 41.9 kD larvicidal toxin gene.

    abstract::A number of strains of the widespread aerobic soil bacterium, Bacillus sphaericus, possess crystalline inclusions of a toxin lethal to a variety of insect (larvae) which are vectors of major tropical diseases. Partial amino acid sequence data from one strain, B. sphaericus 2362 have permitted us to design oligonucleot...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1987.tb00511.x

    authors: Hindley J,Berry C

    更新日期:1987-09-01 00:00:00

  • The two faces of Janus: virulence gene regulation by CovR/S in group A streptococci.

    abstract::The group A streptococcus (GAS) causes a variety of human diseases, including toxic shock syndrome and necrotizing fasciitis, which are both associated with significant mortality. Even the superficial self-limiting diseases caused by GAS, such as pharyngitis, impose a significant economic burden on society. GAS can ca...

    journal_title:Molecular microbiology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2958.2007.05649.x

    authors: Churchward G

    更新日期:2007-04-01 00:00:00

  • Endoplasmic reticulum localized PerA is required for cell wall integrity, azole drug resistance, and virulence in Aspergillus fumigatus.

    abstract::GPI-anchoring is a universal and critical post-translational protein modification in eukaryotes. In fungi, many cell wall proteins are GPI-anchored, and disruption of GPI-anchored proteins impairs cell wall integrity. After being synthesized and attached to target proteins, GPI anchors undergo modification on lipid mo...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/mmi.12626

    authors: Chung D,Thammahong A,Shepardson KM,Blosser SJ,Cramer RA

    更新日期:2014-06-01 00:00:00

  • Molecular mechanisms of virstatin resistance by non-O1/non-O139 strains of Vibrio cholerae.

    abstract::Virstatin is a previously described small molecule inhibitor of Vibrio cholerae virulence. We have demonstrated that the molecule inhibits the activity of the transcriptional activator ToxT, thereby preventing elaboration of the toxin co-regulated pilus (TCP) and cholera toxin in vitro and in vivo in O1 strains of V. ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.2007.05984.x

    authors: Shakhnovich EA,Sturtevant D,Mekalanos JJ

    更新日期:2007-12-01 00:00:00

  • Differential expression of mal genes under cAMP and endogenous inducer control in nutrient-stressed Escherichia coli.

    abstract::LamB glycoporin has an important general role in carbohydrate uptake during growth at low extracellular sugar concentrations. lamB and mal regulon induction during glucose starvation and glucose-limited continuous culture was investigated using lacZ fusions. A low-level burst of lamB induction occurred upon entry into...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1995.tb02397.x

    authors: Notley L,Ferenci T

    更新日期:1995-04-01 00:00:00

  • A gene encoding sn-glycerol 3-phosphate dehydrogenase (NAD+) complements an osmosensitive mutant of Saccharomyces cerevisiae.

    abstract::Osmoregulatory mutants of Saccharomyces cerevisiae with a defect in their capacity to readjust the cell volume/buoyant density after osmotically induced dehydration were enriched by density gradient centrifugation. Colonies derived from cells that remained dense after dehydration were screened for sensitivity to high ...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1993.tb00980.x

    authors: Larsson K,Ansell R,Eriksson P,Adler L

    更新日期:1993-12-01 00:00:00

  • Glucose-induced cAMP signalling in yeast requires both a G-protein coupled receptor system for extracellular glucose detection and a separable hexose kinase-dependent sensing process.

    abstract::In Saccharomyces cerevisiae, glucose activation of cAMP synthesis requires both the presence of the G-protein-coupled receptor (GPCR) system, Gpr1-Gpa2, and uptake and phosphorylation of the sugar. In a hxt-null strain that lacks all physiologically important glucose carriers, glucose transport as well as glucose-indu...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.2000.02125.x

    authors: Rolland F,De Winde JH,Lemaire K,Boles E,Thevelein JM,Winderickx J

    更新日期:2000-10-01 00:00:00

  • A gene involved in both protein secretion during growth and starvation-induced development encodes a subunit of the NADH:ubiquinone oxidoreductase in Myxococcus xanthus.

    abstract::The secretion of numerous proteins during vegetative growth of Myxococcus xanthus, and the multicellular development cycle induced upon starvation of these bacteria, are partially interrelated in so far as mutants impaired in extracellular protein production are unable to undergo development. We have cloned and sequen...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2958.1997.2901655.x

    authors: Laval-Favre K,Letouvet-Pawlak B,Friedrich T,Alexandre J,Guespin-Michel JF

    更新日期:1997-03-01 00:00:00

  • Entamoeba histolytica: electron-dense granule secretion, collagenase activity and virulence are altered in the cytoskeleton mutant BG-3.

    abstract::HM-1:IMSS, a pathogenic strain of Entamoeba histolytica, and its mutant BG-3, identified by resistance to cytochalasin D, were tested for their capacity to: (i) secrete electron-dense granules; (ii) adhere and digest native type I collagen gels; and (iii) produce liver abscesses in new-born hamsters. The results demon...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1994.tb00356.x

    authors: Serrano JJ,de la Garza M,Moreno MA,Tovar R,León G,Tsutsumi V,Muñoz ML

    更新日期:1994-02-01 00:00:00

  • gltF, a member of the gltBDF operon of Escherichia coli, is involved in nitrogen-regulated gene expression.

    abstract::We report here the construction and analysis of insertional mutations in each of the three genes of the gltBDF operon and the nucleotide sequence of the region downstream from gltD. Two open reading frames were identified, the first of which corresponds to gltF. The gltB and gltD genes code for the large and small sub...

    journal_title:Molecular microbiology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2958.1992.tb01450.x

    authors: Castaño I,Flores N,Valle F,Covarrubias AA,Bolivar F

    更新日期:1992-09-01 00:00:00