Enzymatic degradation of 2,6-dichlorophenol by horseradish peroxidase: UV-visible and mass spectrometry characterization of the reaction products [corrected].

Abstract:

:The reaction mechanism of the oxidation of 2,6-dichlorophenol (2,6-DCP) by horseradish peroxidase (HRP) and H2O2 has been investigated and the reaction products have been characterized by UV-visible and mass spectrometry. Evidence for the dimerization of 2,6-DCP to 3,3',5,5'-tetrachloro-4,4'-dihydroxybiphenyl and the subsequent fast oxidation of this product to the corresponding 3,3',5,5'-tetrachlorodiphenoquinone have been collected. The reaction rate was found to decrease markedly as soon as the pH was raised, with a clear inflection point at pH congruent with 6.6-6.9; it also resulted independent from H2O2 concentration. Since the pK(a) for 2,6-DCP is 6.80, the reaction rate might be influenced by the protonation state of the substrate.

journal_name

J Inorg Biochem

authors

Laurenti E,Ghibaudi E,Todaro G,Pia Ferrari R

doi

10.1016/s0162-0134(02)00488-9

subject

Has Abstract

pub_date

2002-09-30 00:00:00

pages

75-81

issue

1

eissn

0162-0134

issn

1873-3344

pii

S0162013402004889

journal_volume

92

pub_type

杂志文章
  • Physico-chemical properties of MnII complexes formed with cis- and trans-DO2A: thermodynamic, electrochemical and kinetic studies.

    abstract:SYNOPSIS:MnII complexes formed with cis- and trans-DO2A (DO2A=1,4,7,10-tetraazacyclododecane-1,4 (or 1,7) -diacetic acid) chelators were investigated by pH-potentiometry, 1H relaxometry, UV-vis spectrophotometry and cyclic voltammetry. The physico-chemical characteristics of MnII complexes of these structure isomers do...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2016.07.018

    authors: Garda Z,Forgács A,Do QN,Kálmán FK,Timári S,Baranyai Z,Tei L,Tóth I,Kovács Z,Tircsó G

    更新日期:2016-10-01 00:00:00

  • Palladium(II) complexes of 2-benzoylpyridine-derived thiosemicarbazones: spectral characterization, structural studies and cytotoxic activity.

    abstract::Palladium(II) complexes of 2-benzoylpyridine thiosemicarbazone (H2Bz4DH) and its N4-methyl (H2Bz4M) and N4-phenyl (H2Bz4Ph) derivatives were obtained and fully characterized. [Pd(2Bz4DH)Cl] (1) crystallizes in the monoclinic space group P21/c with a=11.671(1), b=10.405(1), c=13.124(1), beta=115.60(1) degrees and Z=4; ...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2004.11.022

    authors: Rebolledo AP,Vieites M,Gambino D,Piro OE,Castellano EE,Zani CL,Souza-Fagundes EM,Teixeira LR,Batista AA,Beraldo H

    更新日期:2005-03-01 00:00:00

  • Incorporation of copper into the yeast Saccharomyces cerevisiae. Identification of Cu(I)--metallothionein in intact yeast cells.

    abstract::Copper is an essential metal ion to many living organisms, including mammals, as it mediates a wide variety of important biochemical processes. At elevated concentrations, copper is extremely toxic to host cells. This paradoxical nature of copper has necessitated a highly regulated procedure for its cellular accumulat...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0162-0134(96)00216-4

    authors: Presta A,Stillman MJ

    更新日期:1997-06-01 00:00:00

  • The use of root growth and modelling data to investigate amelioration of aluminium toxicity by silicon in Picea abies seedlings.

    abstract::Three-week-old Picea abies seedlings were grown for 7 days in 100 microM aluminium (Al), combined with 1000 or 2000 microM silicon (Si). Solution pH was adjusted to 4.00, 4.25, 4.50, 4.75, or 5.00. In the absence of Si, solution pH had no effect on the decrease in root growth caused by 100 microM Al. Silicon did not a...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0162-0134(03)00181-8

    authors: Ryder M,Gérard F,Evans DE,Hodson MJ

    更新日期:2003-09-15 00:00:00

  • The adipogenic potential of Cr(III). A molecular approach exemplifying metal-induced enhancement of insulin mimesis in diabetes mellitus II.

    abstract::Insulin resistance is identified through numerous pathophysiological conditions, such as Diabetes mellitus II, obesity, hypertension and other metabolic syndromes. Enhancement of insulin action and\or its complete replacement by insulin-enhancing or insulin-mimetic agents seems to improve treatment of metabolic diseas...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2016.07.015

    authors: Tsave O,Yavropoulou MP,Kafantari M,Gabriel C,Yovos JG,Salifoglou A

    更新日期:2016-10-01 00:00:00

  • Copper(II) inhibits the formation of amylin amyloid in vitro.

    abstract::The amyloidogenic peptide amylin is found associated with pancreatic islet beta-cells and is implicated in the aetiology of type-2 diabetes mellitus. We have used fluorimetry and transmission electron microscopy to investigate in vitro the influence of Al(III), Fe(III), Zn(II) and Cu(II) on amylin amyloid formation un...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2007.09.010

    authors: Ward B,Walker K,Exley C

    更新日期:2008-02-01 00:00:00

  • Mimicking peroxidase activity by a polymer-supported oxidovanadium(IV) Schiff base complex derived from salicylaldehyde and 1,3-diamino-2-hydroxypropane.

    abstract::The polymer-supported oxidovanadium(IV) complex PS-[V(IV)O(sal-dahp)] (2) (PS=chloromethylated polystyrene crosslinked with 5% divinylbenzene, and H3sal-dahp=dibasic pentadentate ligand derived from salicylaldehyde and 1,3-diamino-2-hydroxypropane) was prepared from the corresponding monomeric oxidovanadium(IV) comple...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2015.01.012

    authors: Maurya MR,Chaudhary N,Avecilla F,Correia I

    更新日期:2015-06-01 00:00:00

  • Human ZIP1 is a major zinc uptake transporter for the accumulation of zinc in prostate cells.

    abstract::The prostate gland of humans and other animals accumulates a level of zinc that is 3-10 times greater than that found in other tissues. Associated with this ability to accumulate zinc is a rapid zinc uptake process in human prostate cells, which we previously identified as the hZIP1 zinc transporter. We now provide ad...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0162-0134(03)00249-6

    authors: Franklin RB,Ma J,Zou J,Guan Z,Kukoyi BI,Feng P,Costello LC

    更新日期:2003-08-01 00:00:00

  • Triphenyltin(IV) benzoates with diazenyl/imino scaffold exhibiting remarkable apoptosis mediated by reactive oxygen species.

    abstract::The cytotoxic potency of a series of triphenyltin(IV) compounds of general composition [Ph3Sn(Ln)] (1-6) has been probed in vitro employing MDA-MB-231 (human breast cancer) and HeLa (human cervical cancer) cell lines, where Ln=L1-3; isomeric 2/3/4-{(E)-2-[4-(dimethylamino)phenyl]diazenyl}benzoates and L4-6 are their c...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2017.04.020

    authors: Basu Baul TS,Dutta D,Duthie A,Prasad R,Rana NK,Koch B,Tiekink ERT

    更新日期:2017-08-01 00:00:00

  • The oxidation of rabbit liver metallothionein-II by 5,5'-dithiobis(2-nitrobenzoic acid) and glutathione disulfide.

    abstract::Because metallothionein (MT) may undergo thiol-disulfide or other redox reactions under certain cellular conditions, the partially and completely oxidized products of the reactions of Cd7-MT-II with the electrophile 5,5'-dithiobis(2-nitrobenzoic acid), ESSE, and oxidized glutathione, GSSG, were characterized. Reaction...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/0162-0134(93)80028-8

    authors: Savas MM,Shaw CF 3rd,Petering DH

    更新日期:1993-12-01 00:00:00

  • Desaturation of trans-octadecenoyl-acyl carrier protein by stearoyl-acyl carrier protein delta9 desaturase.

    abstract::Positional isomers of mono-unsaturated 18:1-ACP have been used as substrates for stearoyl-acyl carrier protein delta9 desaturase to test whether a C-H bond abstraction from either the C-9 or C-10 position could lead to rearranged products diagnostic for the production of an allylic radical intermediate. The reconstitu...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0162-0134(99)00199-3

    authors: Broadwater JA,Laundre BJ,Fox BG

    更新日期:2000-01-15 00:00:00

  • Cobalt(III)-ligated peptides as acyl acceptors in peptide synthesis catalyzed by chymotrypsin.

    abstract::The efficiency of cobalt(III)-ligated peptides as acceptor nucleophiles in acyl transfer reactions catalyzed by alpha-chymotrypsin was examined. A series of metallopeptides with the general formula [H-(Gly)n-OCo(NH3)5]2+ (1 < or = n < or = 4) was tested. The aminolysis rate of acyl-chymotrypsin was measured spectropho...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/0162-0134(94)00032-6

    authors: Andreasen MF,Bagger S,Sørensen AM,Wagner K

    更新日期:1995-03-01 00:00:00

  • The reaction between ferrous polyaminocarboxylate complexes and hydrogen peroxide: an investigation of the reaction intermediates by stopped flow spectrophotometry.

    abstract::The reactions of Fe(II)EDTA, Fe(II)DTPA, and Fe(II)HEDTA with hydrogen peroxide near neutral pH have been investigated. All these reactions have been assumed to proceed through an active intermediate, I1, (Formula: see text) where pac is one of the three polyaminocarboxylates mentioned above. I1, whether .OH radical o...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/0162-0134(87)80027-2

    authors: Rush JD,Koppenol WH

    更新日期:1987-03-01 00:00:00

  • Platinum(II) oxalato complexes with adenine-based carrier ligands showing significant in vitro antitumor activity.

    abstract::[Pt(L)(2)(ox)] (1), [Pt(2-OMeL)(2)(ox)] (2), [Pt(3-OMeL)(2)(ox)] (3), [Pt(2,3-diOMeL)(2)(ox)] (4), [Pt(2,4-diOMeL)(2)(ox)] (5), [Pt(3,4-diOMeL)(2)(ox)] (6) and [Pt(3,5-diOMeL)(2)(ox)].4H(2)O (7) platinum(II) oxalato (ox) complexes were synthesized using the reaction of potassium bis(oxalato)platinate(II) dihydrate wit...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2010.02.005

    authors: Starha P,Trávnícek Z,Popa I

    更新日期:2010-06-01 00:00:00

  • Structural and biological aspects of copper (II) complexes with 2-methyl-3-amino-(3H)-quinazolin-4-one.

    abstract::A series of new copper (II) complexes with 2-methyl-3-amino(3H)-quinazolin-4-one (MAQ) and various anions (Cl-, Br-, ClO(-)4, NO(-)3, SCN-, and SO(2-)4 was prepared. Their structures and properties were characterized by elemental analysis, IR, UV-Vis and ESR spectroscopy, molar conductivity, and magnetic moment measur...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0162-0134(96)00122-5

    authors: Ramadan AM

    更新日期:1997-02-15 00:00:00

  • Comparison of wild type neuronal nitric oxide synthase and its Tyr588Phe mutant towards various L-arginine analogues.

    abstract::Crystal structures of nitric oxide synthases (NOS) isoforms have shown the presence of a strongly conserved heme active-site residue, Tyr588 (numbering for rat neuronal NOS, nNOS). Preliminary biochemical studies have highlighted its importance in the binding and oxidation to NO of natural substrates L-Arg and N(omega...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2010.06.001

    authors: Giroud C,Moreau M,Sagami I,Shimizu T,Frapart Y,Mansuy D,Boucher JL

    更新日期:2010-10-01 00:00:00

  • Synergistic anion-directed coordination of ferric and cupric ions to bovine serum transferrin--an inorganic perspective.

    abstract::A series of new iron(III) and copper(II) complexes of bovine serum transferrin (BTf), with carbonate and/or oxalate as the synergistic anion, are presented. The complexes [Fe(2)(CO(3))(2)BTf], [Fe(2)(C(2)O(4))(2)BTf], [Cu(2)(CO(3))(2)BTf] and [Cu(C(2)O(4))BTf] were prepared by standard titrimetric techniques. The oxal...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2003.10.009

    authors: Shongwe MS,Smith R,Marques HM,van Wyk JA

    更新日期:2004-02-01 00:00:00

  • Ferrocenyl naphthalene diimides as tetraplex DNA binders.

    abstract::Seven ferrocenyl naphthalene diimide (FND) ligands were synthesized. Each had a higher affinity for tetraplex DNA than for either single- or double-stranded DNA. The FND binding affinities were >105M-1 in 0.10M AcOH-AcONa or AcOH-AcOK (pH5.5) containing 0.10M NaCl or KCl. The FNDs with the highest binding affinities f...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2016.11.020

    authors: Sato S,Takenaka S

    更新日期:2017-02-01 00:00:00

  • Iron--dioxygen-dependent changes to the biological activities of bleomycin.

    abstract::Antitumor antibiotic bleomycin can bind to and degrade DNA, both in vivo and in vitro. This DNA damaging property in vitro can be related to its ability to chelate ferrous ions under aerobic conditions leading to the formation of "active oxygens," which are thought to be directly responsible for the damage. At present...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/s0162-0134(00)80238-x

    authors: Gutteridge JM,Shute DJ

    更新日期:1981-12-01 00:00:00

  • Evaluation of the biological potential of ruthenium(II) complexes with cinnamic acid.

    abstract::In this work, we present the synthesis and characterization of five new ruthenium compounds with general formula [Ru(L)(dppb)(bipy)]PF6, where L = cinnamic acid derivatives, dppb = 1,4-bis(diphenylphosphino)butane and bipy = 2,2'-bipyridine. The cytotoxicity of the complexes was evaluated against human breast tumor ce...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2020.111021

    authors: Graminha AE,Honorato J,Dulcey LL,Godoy LR,Barbosa MF,Cominetti MR,Menezes AC,Batista AA

    更新日期:2020-05-01 00:00:00

  • Covalent attachment of the heme to Synechococcus hemoglobin alters its reactivity toward nitric oxide.

    abstract::The cyanobacterium Synechococcus sp. PCC 7002 produces a monomeric hemoglobin (GlbN) implicated in the detoxification of reactive nitrogen and oxygen species. GlbN contains a b heme, which can be modified under certain reducing conditions. The modified protein (GlbN-A) has one heme-histidine C-N linkage similar to the...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2017.09.018

    authors: Preimesberger MR,Johnson EA,Nye DB,Lecomte JTJ

    更新日期:2017-12-01 00:00:00

  • Spectroscopic characterization of genotoxic chromium(V) peptide complexes: Oxidation of Chromium(III) triglycine, tetraglycine and pentaglycine complexes.

    abstract::Evidence is growing that metabolites of Cr(III) dietary supplements are partially oxidized to carcinogenic Cr(VI) and Cr(V) in vivo. Hence, we examined oxidations of Cr(III) peptide (triglycine, tetraglycine and pentaglycine) complexes to Cr(VI) and Cr(V) by PbO2 at 37°C and physiological pH values between 3.85 and 7....

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2016.06.015

    authors: Headlam HA,Lay PA

    更新日期:2016-09-01 00:00:00

  • Factors that influence siderophoremediated iron bioavailability: catalysis of interligand iron (III) transfer from ferrioxamine B to EDTA by hydroxamic acids.

    abstract::Deferriferrioxamine B (H3DFB) is a linear trihydroxamic acid siderophore with molecular formula NH2(CH2)5[N(OH)C(O)(CH2)2C(O)NH(CH2)5]2N(OH)C(O)CH3 that forms a kinetically and thermodynamically stable complex with iron(III), ferrioxamine B. Under the conditions of our study (pH = 4.30, 25 degrees C), ferrioxamine B, ...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/0162-0134(83)85010-7

    authors: Monzyk B,Crumbliss AL

    更新日期:1983-08-01 00:00:00

  • The two modes of binding of Ru(phen)(2)dppz(2+) to DNA: thermodynamic evidence and kinetic studies.

    abstract::The binding of Ru(phen)(2)dppz(2+) (dppz=dipyrido[3,2-a:2',3'-c]phenazine) to DNA was investigated at pH 7.0 and 25 degrees C using stopped-flow and spectrophotometric methods. Equilibrium measurements show that two modes of binding, whose characteristics depend on the polymer to dye ratio (C(P)/C(D)), are operative. ...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2006.11.009

    authors: Biver T,Cavazza C,Secco F,Venturini M

    更新日期:2007-03-01 00:00:00

  • Hydrothermal synthesis of copper based nanoparticles: antimicrobial screening and interaction with DNA.

    abstract::Copper based nanoparticles (Cu-based NPs) of different compositions and sizes have been hydrothermally synthesized by varying the reaction time in the presence of the biocompatible surfactants polyoxyethylene (20) sorbitan laurate (Tween 20) and polyethylene glycol 8000 (PEG 8000). Effective control of the above synth...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2013.12.009

    authors: Giannousi K,Lafazanis K,Arvanitidis J,Pantazaki A,Dendrinou-Samara C

    更新日期:2014-04-01 00:00:00

  • Synthesis, characterization and cytotoxic activities of the [RuCl2(NO)(dppp)(L)]PF6 complexes.

    abstract::The synthesis and characterization of ruthenium compounds of the type [RuCl(2)(NO)(dppp)(L)]PF(6) [dppp=1,3-bis(diphenylphosphino)propane; L=pyridine, 4-methylpyridine, 4-phenylpyridine and dimethyl sulfoxide] are described. The complexes were characterized by elemental analysis, UV/Vis and infrared spectroscopy, cycl...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2009.12.015

    authors: Golfeto CC,Von Poelhsitz G,Selistre-de-Araújo HS,de Araujo MP,Ellena J,Castellano EE,Lopes LG,Moreira IS,Batista AA

    更新日期:2010-05-01 00:00:00

  • 1H, 13C NMR, and electronic absorption spectroscopic studies of the interaction of cyanide with aurothiomalate.

    abstract::The reaction between cyanide and aurothiomalate (Autm) has been studied by 1H and 13C NMR spectroscopy and by uv spectroscopy. At cyanide:Autm ratios greater than or equal to 2, aurocyanide, [Au(CN)2]-, is the sole product but was also produced at lower ratios. Two intermediates were also identified. These were a mixe...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/0162-0134(85)80010-6

    authors: Graham GG,Bales JR,Grootveld MC,Sadler PJ

    更新日期:1985-11-01 00:00:00

  • Intercalation of tetracationic metalloporphyrins and related compounds into DNA.

    abstract::Electron paramagnetic resonance (e.p.r.) studies of thin DNA-chelate films indicate that both the water soluble planar macrocyclic chelates, cobalt (II) tetra (2,3-N-methylpyridinium) porphyrazine (cobalt (II) TMTPPA), and vanadyl (II) 5,10,15,20-tetra-(4-methylpyridinium) porphyrin (Vo (II) TMTPyP) can bind to calf t...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/0162-0134(88)85021-9

    authors: Dougherty G

    更新日期:1988-10-01 00:00:00

  • Effect of redox partner binding on CYP101D1 conformational dynamics.

    abstract::We have compared the thermodynamics of substrate and redox partner binding of P450cam to its close homologue, CYP101D1, using isothermal titration calorimetry (ITC). CYP101D1 binds camphor about 10-fold more weakly than P450cam which is consistent with the inability of camphor to cause a complete low- to high-spin shi...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2018.02.013

    authors: Batabyal D,Poulos TL

    更新日期:2018-06-01 00:00:00

  • Molecular recognition patterns of 2-aminopurine versus adenine: a view through ternary copper(II) complexes.

    abstract::In contrast to the comprehensive structural information about metal complexes with adenine, the corresponding to its isomer 2-aminopurine (H2AP) is extremely poor. With the aim to rationalize the metal binding pattern of H2AP, we report the molecular and/or crystal structure of four novel compounds with various iminod...

    journal_title:Journal of inorganic biochemistry

    pub_type: 杂志文章

    doi:10.1016/j.jinorgbio.2011.05.009

    authors: Domínguez-Martín A,Choquesillo-Lazarte D,González-Pérez JM,Castiñeiras A,Niclós-Gutiérrez J

    更新日期:2011-08-01 00:00:00