Abstract:
:Estrogen sulfotransferase (EST) catalyzes the sulfoconjugation and inactivation of the steroid hormone estrogen. It is known previously that EST is expressed abundantly in Leydig cells of the testis. We recently have shown that male mice with targeted EST gene disruption developed age related Leydig cell and seminiferous tubule abnormalities as a consequence of increased local estrogen stimulation. In the same study, we also found that epididymal sperm isolated from the mutant mice had significantly reduced motility, but whether this reflected impaired epididymal function or was secondary to the testicular lesions was not known. The purpose of the current study was to investigate if EST is normally present in the mouse epididymis and/or other parts of the male reproductive tract where, as in testis, it may play a role in regulating local estrogen homeostasis. We describe here that EST is expressed in the epithelium of corpus and cauda but not caput regions of the mouse epididymis. It is also expressed in the luminal epithelium and smooth muscle cells of the vas deferens but was present at very low levels, if at all, in the prostate or seminal vesicle/ coagulating gland. Hypophysectomy, castration, and epididymal ligation experiments, together with the use of an androgen receptor antagonist, established that EST expression in the epididymis and vas deferens is critically dependent on pituitary hormone(s) and androgen but not on other factors in the testicular fluid. Administration of exogenous estradiol to mice with surgically ligated epididymis resulted in a more pronounced reduction in sperm motility in EST mutant mice than in wild-type mice. We conclude that EST is discretely expressed and regulated in the male reproductive tract and plays a physiological role in maintaining the functional integrity of the epididymis by regulating luminal estrogen homeostasis.
journal_name
Endocrinologyjournal_title
Endocrinologyauthors
Tong MH,Song WCdoi
10.1210/endo.143.8.8943subject
Has Abstractpub_date
2002-08-01 00:00:00pages
3144-51issue
8eissn
0013-7227issn
1945-7170journal_volume
143pub_type
杂志文章相关文献
ENDOCRINOLOGY文献大全abstract::A serum-free, hormone- and factor-supplemented, defined medium was developed which maintains functional activity of primary cultures of adult islet cells and continuous islet cell lines. Medium supplements examined included proteose peptone (PP), transferrin (TrFe), insulin-like growth factor-I (IGF-I), an insulinotro...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-126-4-1895
更新日期:1990-04-01 00:00:00
abstract::To study the interaction of TSH receptor (TSHR) autoantibodies with receptor protein; it is necessary first to express the receptor in the proper conformation including the formation of correct disulfide bridges. However, the reducing environment of the Escherichia coli (E. coli) cytoplasm prevents the generation of p...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.2.4920
更新日期:1997-02-01 00:00:00
abstract::Rats were made unilaterally cryptorchid by cutting the gubernaculum testis at birth. At 100 days age, the rats were injected with 600 IU/kg hCG. A biphasic testosterone response was seen in the scrotal (Scr) testis in response to hCG, with maxima at 1 h and 3 days after injection. The acute peak of testosterone was of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-2-550
更新日期:1984-08-01 00:00:00
abstract::Kisspeptin regulates reproductive events, including puberty and ovulation, primarily via GnRH neurons. Prolonged treatment of prepubertal striped bass females with kisspeptin (Kiss) 1 or Kiss2 peptides failed to enhance puberty but suggested a gnrh-independent pituitary control pathway. Kiss2 inhibited, but Kiss1 stim...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2013-1894
更新日期:2014-05-01 00:00:00
abstract::A rare 20K isoform of GH-V (here abbreviated as GHv) was discovered in 1998. To date, only 1 research article has characterized this isoform in vivo, observing that GHv treatment in male high-fat fed rats had several GH-like activities, but unlike GH lacked diabetogenic and lactogenic activities and failed to increase...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa097
更新日期:2020-08-01 00:00:00
abstract::Pancreatic beta-cells and HIT-T15 cells exhibit oscillations of cytosolic calcium ([Ca2+]i) that are dependent on glucose metabolism and appear to trigger pulsatile insulin secretion. Significantly, differences in the pattern of this [Ca2+]i oscillatory activity may have important implications for our understanding of...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.135.1.8013370
更新日期:1994-07-01 00:00:00
abstract::Inhibin was first characterized in mammals as a gonadal dimeric protein that inhibited pituitary follicle-stimulating hormone (FSH) secretion. As in mammals, the inhibin-specific α subunit (INHA/Inha/inha) has also been characterized in teleosts; however, its functions and physiological importance in fish reproduction...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endocr/bqaa184
更新日期:2020-12-01 00:00:00
abstract::Circulating angiotensin II (ANGII) elicits water intake and activates the hypothalamic-pituitary-adrenal (HPA) axis by stimulating angiotensin type 1 receptors (AT1Rs) within circumventricular organs. The subfornical organ (SFO) and the organum vasculosum of the lamina terminalis (OVLT) are circumventricular organs th...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2008-0477
更新日期:2008-12-01 00:00:00
abstract::The effect of beta-endorphin upon plasma arg8-vasopressin release was studied in vivo in rabbits and in vitro in rat neural lobes. Following intravenous administration of 200 mug/kg synthetic beta-endorphin plasma AVP rose significantly by five minutes after injection and remained elevated for twenty-five minutes comp...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-101-5-1643
更新日期:1977-11-01 00:00:00
abstract::Up-regulation of maternal islet function is essential to accommodate the increased demand for insulin during pregnancy. Previously, we suggested that lactogenic activity regulates islet function during pregnancy. However, this hypothesis was based on the effect of homologous PRLs on islets, since the homologous placen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.132.2.8425500
更新日期:1993-02-01 00:00:00
abstract::Ghrelin is a peptide hormone primarily produced in the previously unidentified X/A endocrine cells of the stomach. Extensive studies have focused on the effects of ghrelin on growth hormone release and appetite regulation. However, the mechanisms regulating ghrelin secretion are less understood. In the present study, ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2012-1040
更新日期:2012-08-01 00:00:00
abstract::Starvation depresses thyroid gland function. In addition, the peripheral turnover of thyroxine (T4) is reduced, in part due to decreased fecal elimination of T4. The present studies were performed to determine if starvation also affects the deiodinative pathway for T4 degradation. Rats were isotopically equilibrated w...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-96-6-1525
更新日期:1975-06-01 00:00:00
abstract::The effects of cilostazol, a dual inhibitor of type 3 phosphodiesterase and adenosine uptake, on ion currents were investigated in pituitary GH(3) cells and pheochromocytoma PC12 cells. In whole-cell configuration, cilostazol (10 microm) reversibly increased the amplitude of Ca(2+)-activated K(+) current [I(K(Ca))]. C...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-1430
更新日期:2004-03-01 00:00:00
abstract::The present study determined whether the reduction in serum progesterone (P4) concentrations which follows the administration of the antiestrogen ethamoxytriphetol [1-(rho-2-diethylaminoethoxyphenyl)1-phenyl-2-rho-methoxyphenyl ethanol (MER-25)] to pregnant baboons reflects a decline in placental and/or luteal functio...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-5-1717
更新日期:1984-11-01 00:00:00
abstract::The role of the paraventricular nucleus-dorsal anterior hypothalamus (PVN-DAHA) in the control of anterior pituitary gland secretion of FSH and LH in castrated male and female rats was examined. Bilateral radiofrequency lesions of the PVN-DAHA in chronically ovariectomized (OVX) rats lowered plasma FSH levels by 33% (...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-115-6-2473
更新日期:1984-12-01 00:00:00
abstract::We have shown that nonlactogenic rat (r) GH is far more potent than rPRL in inducing rat mammary development. To determine the relative roles of GH and PRL in mammary development and their mechanisms of action, we have compared the abilities of a group of native and mutant GHs, PRLs, and placental lactogens (PLs) to i...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.4.8404600
更新日期:1993-10-01 00:00:00
abstract::Although the main role of prolactin (PRL) in pregnant rodents is to sustain progesterone production by the corpus luteum, progesterone treatment of PRL or PRL receptor (PRL-R) null mice is unable to prevent fetal loss. We have previously shown that the rat decidua is a site of PRL production and action. In this report...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2006-1643
更新日期:2007-05-01 00:00:00
abstract::Estrogen increases the alpha 1-adrenergic contractile sensitivity of the rabbit uterus. Since estrogen treatment increases prostaglandin (PG) production by the perfused rabbit uterus, and PGs contribute to the alpha 1-adrenergic contractile response, we postulated that estrogen's effects on PG production or response m...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-3-1436
更新日期:1991-09-01 00:00:00
abstract::Synthesis of uteroglobin in rabbit uterine epithelial cells during early pregnancy is induced by progesterone and apparently suppressed by estrogen. To assess whether the steroid hormones exert regulatory effects on the expression of the uteroglobin gene, we performed transcriptional rate assays in endometrial nuclei ...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-112-3-871
更新日期:1983-03-01 00:00:00
abstract::Bovine TSH (bTSH) was subjected to reverse phase HPLC using a Synchropak RP-P C18 column. Elution was with a linear gradient of 0-50% 1-propranol in 0.1% trifluoroacetic acid. Individual fractions were assayed for TSH bioactivity by the cultured thyroid cell cAMP response assay. Optimum separation of biologically acti...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-116-4-1379
更新日期:1985-04-01 00:00:00
abstract::Although mothers against dpp (MAD) and its related proteins (MADR) are believed to be important components of the cell signaling pathway for the transforming growth factor beta (TGFbeta) superfamily, the presence and regulation of these signaling molecules in ovarian cells by TGFbeta is not known. In the present studi...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.138.9.5376
更新日期:1997-09-01 00:00:00
abstract::Acute nephrectomy seriously impairs hypovolemic adrenal epinephrine (E) release in the anesthetized dog. That systemically delivered angiotensin II totally restores E release to acutely anephric dogs is equally clear, but the dose-response relationship of this angiotensin II effect is not known. Adrenal secretion rate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-117-5-1920
更新日期:1985-11-01 00:00:00
abstract::Steady state levels of the mRNAs for alpha 1B, beta 1- and beta 2-adrenergic receptors (alpha 1BAR, beta 1AR, beta 2AR) were quantified by DNA excess solution hybridization assays in the heart, lungs, and liver of rats. Tissues for RNA extraction were obtained from euthyroid and thyroidectomized rats and from thyroide...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-2-1116
更新日期:1991-08-01 00:00:00
abstract::Recent evidence has suggested that a circadian rhythm exists for plasma beta-endorphin-like immunoreactivity. The purpose of the present study was to examine the long term effects of surgical trauma on plasma beta-endorphin dynamics. Blood samples for RIA were obtained from female baboons every 4 h for three 48-h peri...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-120-5-1734
更新日期:1987-05-01 00:00:00
abstract::Mammary epithelial cells isolated from pregnant, nonlactating heifers were grown in vitro using collagen substrates. Using these systems, the truncated form of insulin growth factor-1 (IGF-1) (des-3-IGF-1), IGF-1, and IGF-2 all stimulated a significant (0.5 to 1 fold) increase in cell proliferation (des-3-IGF-1 greate...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-129-2-671
更新日期:1991-08-01 00:00:00
abstract::The fate of cell-bound [125I]iodo-human GH ([125I]iodo-hGH) was studied in monolayer cultures of hepatocytes from pregnant and nonpregnant rats. Both the binding of [125I]iodo-hGH and its degradation were significantly higher in cells from pregnant than from nonpregnant rats. The positive correlation between the numbe...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-114-5-1475
更新日期:1984-05-01 00:00:00
abstract::The present experiments investigated the time course of maternal modulation of GH secretion and examined the possible role of milk in the regulation of GH secretion in neonatal rats. Serum GH concentrations in neonatal rats were high at birth and declined over time postpartum. Separation of rat pups from their mothers...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo-125-3-1326
更新日期:1989-09-01 00:00:00
abstract::IGF-I and IGF-II play important roles in growth and development via interactions with cell-surface receptors; however, in nature, IGFs are sequestered by at least six soluble, high-affinity IGF-binding proteins (IGFBPs), namely IGFBPs 1-6. Herein, we demonstrate that the stromal cell-derived extracellular matrix-degra...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2003-0636
更新日期:2004-02-01 00:00:00
abstract::In the course of previous studies using continuous monitoring of the electrophysiological correlates of GnRH pulse generator activity, characterized by episodic increases in hypothalamic multiunit electrical activity (MUA volley), it was noticed that the nocturnal slowing of pulse generator frequency was an acute phen...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/endo.133.4.8404590
更新日期:1993-10-01 00:00:00
abstract::The neuropeptide kisspeptin (Kiss1) is integral to the advent of puberty and the generation of cyclical LH surges. Although many complex actions of Kiss1 are known, the mechanisms governing the processing/regulation of this peptide have not been unveiled. The metallo enzyme, endopeptidase 24.15 (thimet oligopeptidase)...
journal_title:Endocrinology
pub_type: 杂志文章
doi:10.1210/en.2015-1580
更新日期:2016-02-01 00:00:00