Abstract:
:The base pair stack within double helical DNA provides an effective medium for charge transport. The DNA pi-stack mediates oxidative DNA damage over long molecular distances in a reaction that is exquisitely sensitive to the sequence-dependent conformation and dynamics of DNA. A mixture of tunneling and hopping mechanisms have been proposed to account for this long-range chemistry, which is gated by dynamical variations within the stack. Electrochemical sensors have also been developed, based upon the sensitivity of DNA charge transport to base pair stacking, and these sensors provide a completely new approach to diagnosing single base mismatches in DNA and monitoring protein-DNA interactions electrically. DNA charge transport, furthermore, may play a role within the cell and, indeed, oxidative damage to DNA from a distance has been demonstrated in the cell nucleus. As a result, the biological consequences of and opportunities for DNA-mediated charge transport now require consideration.
journal_name
Curr Opin Struct Bioljournal_title
Current opinion in structural biologyauthors
Boon EM,Barton JKdoi
10.1016/s0959-440x(02)00327-5subject
Has Abstractpub_date
2002-06-01 00:00:00pages
320-9issue
3eissn
0959-440Xissn
1879-033Xpii
S0959440X02003275journal_volume
12pub_type
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