Abstract:
:Although rat models implanted with C6 glioma cells have been widely utilized for the assessment of new therapeutic modalities, a convenient in vivo model is still required. We implanted C6 glioma cells into the brains and the abdominal subcutaneous regions of Wistar rats, and evaluated the volumes of the growing tumors. The cultures of 5 x 10(6) cells had successfully formed tumors at 100% (52/52) of sites in the subcutaneous regions on day 5 after implantation. It was easy to measure the visible tumors with a sliding caliper. The tumor volume at the subcutaneous region reached a maximum volume (950 mm3+/-167 SE) on day 15, after which it diminished. In contrast, tumors implanted in the intracerebral region showed a maximum volume on day 20 after the implantation of 1 x 10(5) cultured cells. Pathological examinations of the subcutaneous and intracranial tumors on day 15 showed similar findings exhibiting high nuclear cell ratio, mitosis and pseudopalisading with small populations of GFAP positive cells. The results suggested that rat C6 glioma models with implantation into the subcutaneous abdomen are available from day 5 to day 15 as a convenient model for the assessment of anti-proliferative drugs. The usefulness and the limitations of the rat C6 glioma model are discussed in this manuscript.
journal_name
Neurol Resjournal_title
Neurological researchauthors
Watanabe K,Sakamoto M,Somiya M,Amin MR,Kamitani H,Watanabe Tdoi
10.1179/016164102101200221subject
Has Abstractpub_date
2002-07-01 00:00:00pages
485-90issue
5eissn
0161-6412issn
1743-1328journal_volume
24pub_type
杂志文章abstract::The present study was designed to investigate the beneficial effects of cornel iridoid glycoside (CIG), a main component extract from Cornus officinalis, on neurotrophin expression in mouse experimental autoimmune encephalomyelitis (EAE), a classical model of multiple sclerosis (MS). After EAE initiation, CIG was intr...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.2016.1200766
更新日期:2016-09-01 00:00:00
abstract::L-644,711 is a novel anion channel inhibitor which has previously been shown to decrease brain injury in two related rabbit models of cerebral ischaemia. We hypothesize two mechanisms of action of L-644,711 for its salutary effects on cerebral ischaemia: inhibition of neutrophil function and prevention of excitotoxin ...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1992.11740011
更新日期:1992-03-01 00:00:00
abstract::The endothelial lining of blood vessels plays an important role in the control of arterial tone by releasing a number of vasoactive factors. Among these, nitric oxide (NO) or a NO containing moiety, some cyclooxygenase products such as prostacyclin (PGl2) and a still hypothetical hyperpolarizing factor may induce dila...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1997.11740851
更新日期:1997-10-01 00:00:00
abstract:OBJECTIVES:Hourly neurologic assessments for traumatic brain injury (TBI) in the critical care setting are common practice but prolonged use may actually be harming patients through sleep deprivation. We reviewed practice patterns at our institution in order to gain insight into the role of frequent neurological assess...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/1743132813Y.0000000285
更新日期:2014-02-01 00:00:00
abstract:INTRODUCTION:Here, we investigated inflammatory signs of peripheral nerves in leptin-deficient obese ob/ob mice and the modulating effects of the exogenous iron load. METHODS:Ob/ob and ob/+ control mice were fed with high, standard, or low iron diet for four months. RESULTS:We found intraepidermal nerve fiber degener...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.2018.1564191
更新日期:2019-04-01 00:00:00
abstract::The possible role of nitric oxide (NO) on vibrissa-stimulated increase of regional cerebral cerebral blood flow (rCBF) and cerebral metabolic rate of glucose (rCMRglu) was investigated in conscious Wistar rats by using an inhibitor of NO synthase, NG-nitro-L-arginine (NOLAG) at a concentration of 30 mg/kg. In vivo aut...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1993.11740103
更新日期:1993-02-01 00:00:00
abstract::Recovery of motor function following stroke is believed to be supported, at least in part, by functional compensation involving residual neural tissue. The present study used a rodent model of focal ischemia and intracortical microstimulation (ICMS) to examine the behavioral and physiological effects of cortical stimu...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/016164103771953862
更新日期:2003-12-01 00:00:00
abstract::Localization of the pathological structures in relation to the surrounding anatomy and understanding of the surgical anatomy are probably the most important keys to successful neurosurgery. Image-guided surgery is an important tool for understanding an individual's anatomy and for precisely locating the lesion. Head r...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1998.11740481
更新日期:1998-01-01 00:00:00
abstract:OBJECTIVE:The role of inflammation in cognitive alterations in a post-operative setting is still not fully understood. Surgical interventions can cause systemic inflammations which eventually can induce neuroinflammation. However, the main causes of functional changes after surgery are still elusive. In this study, we ...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.2016.1242452
更新日期:2016-11-01 00:00:00
abstract::Previous work has shown that unsaturated fatty acid components of model membrane phospholipids in vitro, damaged via a free radical mechanism, are protected by the presence of cholesterol in these membranes. The participation of these membrane lipids in the pathogenesis of traumatic injury to brain was studied in vivo...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1981.11739608
更新日期:1981-01-01 00:00:00
abstract:OBJECTIVE:Surgical resection of complex glomus vagale tumors can be complicated by extensive blood loss and might require surgical sacrifice of an encased internal carotid artery. METHODS:A young patient presented with mass effect from glomus valage tumor. Computerized tomography angiography showed an encased interna...
journal_title:Neurological research
pub_type: 杂志文章,评审
doi:10.1179/1743132812Y.0000000036
更新日期:2012-07-01 00:00:00
abstract::Induction of stress proteins is thought to be important in the protection of cells from a variety of environmental insults including heat, hypoxia and ischemia. The aim of this study was to compare the mechanism of induction of heat shock protein 72 (HSP72) in primary cultures of murine cortical astrocytes by heat and...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1996.11740380
更新日期:1996-02-01 00:00:00
abstract::As early as 1 to 3 hr after cord transection, proliferation of many reactive oligodendrocytes was observed. Bundles of microfilaments and microtubules were observed in those cells that sent out long, complex pseudopodlike processes near the area of injury. These reactive oligodendrocytes may be comparable to Vaughn's ...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1980.11739566
更新日期:1980-01-01 00:00:00
abstract::Endovascular reconstruction of basilar artery (BA) apex aneurysms has been augmented by adjunctive techniques such as balloon and stent assistance. We present three cases of a wide-necked BA apex aneurysm involving the bilateral P1 segments of both posterior cerebral arteries (PCAs) treated by placement of BA to PCA s...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/016164104225013969
更新日期:2004-04-01 00:00:00
abstract:OBJECTIVES:One of the main obstacles of electrode implantation in epilepsy surgery is the electrode shift between implantation and the day of explantation. We evaluated this possible electrode displacement using intraoperative MRI (iopMRI) data and CT/MRI reconstruction. METHODS:Thirteen patients (nine female, four ma...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.2018.1484588
更新日期:2018-10-01 00:00:00
abstract::There have been reports that superior cervical ganglionectomy (SCG) is effective as a treatment for cerebral vasospasm following subarachnoid haemorrhage (SAH). On the other hand, denervation supersensitivity is supposed to play an important role in vasospasm. The two accounts contradict each other. To investigate thi...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.1988.11739836
更新日期:1988-09-01 00:00:00
abstract:OBJECTIVE:Repetitive magnetic stimulation (rMS) is mainly used in transcranial applications. Only a few works have described its potential peripheral use. The aim of this investigation was to determine if conditioning peripheral (paravertebral) rMS of the cervical nerve roots in a group of healthy subjects induces chan...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/174313208X297959
更新日期:2008-09-01 00:00:00
abstract::No marker that predicts accurately the time of occurrence of cerebral vasospasm due to subarachnoid hemorrhage (SAH) has been reported. In the present study, membrane-bound tissue factor (mTF) and myelin basic protein (MBP) concentrations in cerebrospinal fluid (CSF) were evaluated as a predictor of the time of occurr...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/016164101101199225
更新日期:2001-10-01 00:00:00
abstract::Objective: To assess the neuropsychological status of pediatric multiple sclerosis (MS) patients and its relationship with clinical variables in a longitudinal study.Methods: Patients with MS (n = 46) and age- and gender-matched healthy control subjects (HCs, n = 53) were given tests of non-verbal reasoning, attention...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.2019.1710417
更新日期:2020-02-01 00:00:00
abstract::Decreased metabolic rate may precede cognitive impairment in Alzheimer's disease (AD) and is thus an early occurring hallmark. Several observations in post-mortem brain indicate that activated neurons are better able to withstand aging and AD, a phenomenon paraphrased by us as 'use it or lose it'. Moreover, a number o...
journal_title:Neurological research
pub_type: 杂志文章,评审
doi:10.1179/016164103101201959
更新日期:2003-09-01 00:00:00
abstract:BACKGROUND:Diffusion-weighted imaging (DWI) abnormalities can frequently be detected after carotid endarterectomy (CEA) and carotid angioplasty with stent placement (CAS) of the carotid arteries. We looked for possible predictors for the development of DWI lesions during the intervention. METHODS:We investigated 41 pa...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/016164105X49391
更新日期:2006-07-01 00:00:00
abstract:OBJECTIVE:Cerebral small vessel disease (CSVD) describes a syndrome of neuroimaging, pathological, and associated clinical features caused by small intracranial vascular lesions, which commonly coexists with large artery atherosclerosis (LAA) and has been identified as a major cause of motor impairment over time. In th...
journal_title:Neurological research
pub_type: 杂志文章,评审
doi:10.1080/01616412.2018.1446283
更新日期:2018-05-01 00:00:00
abstract:INTRODUCTION:Cerebrospinal fluid (CSF) oligoclonal bands (OB) imply intrathecal immunoglobulin synthesis and B-cell immune process. There is scarce evidence of OB having a role in disease prognosis. The objective of the present study was to determine OB's prognostic value regarding disease progression. METHODS:Betwee...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/1743132812Y.0000000088
更新日期:2012-11-01 00:00:00
abstract::Endolymphatic sac tumors are rare, low-grade adenocarcinomas of the petrous bone. These tumors are highly vascularized and surgical removal is often challenging. We present a staged endovascular-neurosurgical approach consisting of stent-assisted angioplasty of the petrous carotid, selective endovascular embolization ...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/016164103101201436
更新日期:2003-04-01 00:00:00
abstract:OBJECTIVES:In order to avoid occurrence of post-operative hygromas in specific hydrocephalus patients being at high risk of overdrainage, a combination of programmable valve and anti-gravity device is widely recommended. We analyzed our series of hydrocephalus patients implanted with such a shunt configuration focusin...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/1743132811Y.0000000079
更新日期:2012-04-01 00:00:00
abstract::Sixty-five years old patient suffering from acute stroke was treated by rTPA intravenously. TCD monitoring of both middle cerebral arteries (MCA) was carried out simultaneously with administration of rTPA. Seven microemboli were found in right and four in left MCA. Duplex ultrasound, CT angiography and digital subtrac...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/174313208X289589
更新日期:2008-09-01 00:00:00
abstract:OBJECTIVE:This study investigated the role of matrix metalloproteinase-9 (MMP-9) in early brain injury after subarachnoid hemorrhage (SAH). METHOD:Sprague-Dawley male rats (n=36) weighing between 250 and 300 g were used. SAH was produced by injecting autologous arterial blood into the pre-chiasmatic cistern. MMP-9 pro...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/016164109X12478302362491
更新日期:2010-09-01 00:00:00
abstract::The purpose of this study was to determine the efficacy of nicardipine prolonged-release implants for preventing vasospasm in a canine SAH model in a dose-escalating placebo-controlled blind fashion. Drug-release kinetics of copoly(lactic/glycolic acid) pellet containing nicardipine were evaluated in vitro. In vivo, 1...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1080/01616412.2000.11740733
更新日期:2000-09-01 00:00:00
abstract:OBJECTIVES:We have investigated whether magnetic stimulation of the sural nerve can evoke a flexor reflex recorded from the ipsilateral short head of the biceps femoris muscle. METHODS:The sural nerve was subjected to magnetic stimulation as well as by single-pulse electrical stimulation in healthy subjects. RESULTS:...
journal_title:Neurological research
pub_type: 杂志文章,随机对照试验
doi:10.1179/1743132810Y.0000000026
更新日期:2011-07-01 00:00:00
abstract::Cyclic GMP (cGMP) mediates smooth muscle relaxation in the central nervous system. In subarachnoid hemorrhage (SAH), decreases in intrinsic nitric oxide (NO) cause cerebral vasospasms due to the regulation of cGMP formation by NO-mediated pathways. As phosphodiesterase type V (PDE V) selectively hydrolyzes cGMP, we hy...
journal_title:Neurological research
pub_type: 杂志文章
doi:10.1179/016164102101200447
更新日期:2002-09-01 00:00:00