Abstract：
:There is a large body of evidence indicating important interactions between the adenosine and opioid systems in regulating pain at both the spinal and supraspinal level. Mice lacking the mu-opioid receptor (MOR) gene have been successfully developed and the animals show complete loss of analgesic responses to morphine as well as differences in pain sensitivity. To investigate if there are any compensatory alterations in adenosine systems in mutant animals, we have carried out quantitative autoradiographic mapping of A(1) and A(2A) adenosine receptors and nitrobenzylthioinosine (NBTI) sensitive adenosine transporters in the brains and spinal cords of wild type, heterozygous and homozygous mu-opioid receptor knockout mice. Adjacent coronal sections were cut from the brains and spinal cords of +/+, +/- and -/- mice for the determination of binding of [3H]DPCPX, [3H]CGS21680 or [3H]NBTI to A(1) and A(2A) adenosine receptors and NBTI-sensitive adenosine transporters, respectively. A small but significant reduction in [3H]DPCPX and [3H]NBTI binding was detected in mutant mice brains but not in spinal cords. No significant change in A(2A) binding was detected in mu-opioid receptor knockout brains. The results suggest there may be functional interactions between mu-receptors and A(1) adenosine receptors as well as NBTI-sensitive adenosine transporters in the brain but not in the spinal cord.
journal_name
Brain Resjournal_title
Brain researchauthors
Bailey A,Matthes H,Kieffer B,Slowe S,Hourani SM,Kitchen Idoi
10.1016/s0006-8993(02)02536-2subject
Has Abstractpub_date
2002-07-05 00:00:00pages
68-79issue
1eissn
0006-8993issn
1872-6240pii
S0006899302025362journal_volume
943pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Experiments were carried out to determine if stimuli which augment preganglionic nerve activity to sympathetic neurons, and thereby cause trans-synaptic induction, increase the retrograde transport of nerve growth factor (NGF). It was found that nerve activity had no effect on retrograde transport of [125I]NGF. It was...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90701-7
更新日期：1979-12-14 00:00:00
abstract：:Headache medicine is primarily dependent on patients' subjective reports of pain, which are assessed at diagnosis and throughout the duration of treatment. There is a need for an objective, quantitative biological measurement of headache pain severity. In this study, quantitative sensory testing (QST) was conducted vi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.12.043
更新日期：2013-03-01 00:00:00
abstract：:In the present experiment we use a rat model of traumatic brain injury to evaluate the ability of low-pressure hyperbaric oxygen therapy (HBOT) to improve behavioral and neurobiological outcomes. The study employed an adaptation of the focal cortical contusion model. 64 Male Long-Evans rats received unilateral cortica...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.06.105
更新日期：2007-10-12 00:00:00
abstract：:The ventral tegmental area has been suggested as a possible site of action for the rewarding effect of opiates on the basis of the fact that local morphine injections in this but not other regions are rewarding. That this is a necessary and not just a sufficient site of opiate rewarding action was suggested by the fac...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(83)91232-5
更新日期：1983-01-03 00:00:00
abstract：:The two human brain beta-galactosidases were solubilized and fractionated by Sephadex G-200 gel filtration, free from each other. Substrate specificities of the two enzymes were examined for galactosylceramide, lactosyl-[N-stearoyl]ceramide, lactosyl-[N-lignoceroyl]ceramide, galactosyl-N-acetylgalactosaminyl-[N-stearo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(77)90298-0
更新日期：1977-02-18 00:00:00
abstract：:In the present studies, we have evaluated the effects of N-[4-(2-¿[(3-Chlorophenyl)methyl]amino¿ethyl)phenyl]-2-thiophenecarbo ximidamide dihydrochloride (ARL 17477) on recombinant human neuronal NOS (nNOS) and endothelial NOS (eNOS). We then carried out pharmacokinetic studies and measured cortical nitric oxide synth...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02471-9
更新日期：2000-07-21 00:00:00
abstract：:Physical activity has been proposed as a behavior intervention that promotes mental health and some of the benefits induced by exercise have been related to the glutamatergic system. Indeed, glutamate is the most abundant excitatory neurotransmitter in brain. Thus, we evaluated if voluntary exercise in mice could modu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2005.09.038
更新日期：2005-12-14 00:00:00
abstract：:Fast cyclic voltammetry (FCV) at carbon fibre microelectrodes was used to monitor stimulated noradrenaline (NA) efflux in slices of the ventral part of the rat bed nucleus of stria terminalis (BSTV) superfused with artificial cerebrospinal fluid at 32 degrees C. NA efflux was evoked by local electrical stimulation (tr...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(93)91498-h
更新日期：1993-04-02 00:00:00
abstract：:Enkephalin immunoreactive neurons within the nucleus tractus solitarius (NTS) were found to project to the parabrachial nucleus of the cat with the use of a combination of immunohistochemistry and retrograde transport of horseradish peroxidase. Double labelled neurons were located in the medial, parvocellular and comm...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91521-1
更新日期：1988-03-01 00:00:00
abstract：:In order to establish possible stimulatory effects of increasing plasma concentrations of substance P (SP) on the arginine vasopressin (AVP) and/or oxytocin (OT) secretion, successively increasing doses of SP(0.5, 1 and 1.5 pmol/kg-1/min-1; each dose for 20 min) were infused in 7 normal men. Plasma AVP and OT levels w...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(92)90387-o
更新日期：1992-01-08 00:00:00
abstract：:Studies related to brain oscillations pervade the neuroscience literature. Functional correlates of oscillations in the delta, theta, alpha, beta and gamma frequency windows are interpreted in several ways. However, only a small number of reports indicate the need for overall interpretations by integrating all type of...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2008.06.102
更新日期：2008-10-15 00:00:00
abstract：:Electrical stimulation of the mesencephalic locomotor region (MLR) in the precollicular-postmammillary transected cat is known to induce controlled locomotion on a treadmill. We have been able to induce and block locomotion in this preparation by using localized infusions of transmitters and their agonists and antagon...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90006-x
更新日期：1985-03-18 00:00:00
abstract：:The effects of forced swimming for 30 min on extracellular 5-hydroxytryptamine (5-HT) levels were examined in five brain regions in rats using in vivo microdialysis. A single dialysis probe was implanted under surgical anesthesia into either the striatum, ventral hippocampus, frontal cortex, amygdala, or lateral septu...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00349-u
更新日期：1995-06-05 00:00:00
abstract：:Prolonged seizures have long been known to be associated with cell injury and cell death in brain. Such seizure-related neuronal injury has been assumed to be mediated by glutamate, the same excitatory amino acid in the central nervous system which propagates the seizure itself. Elevated extracellular concentrations o...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00658-0
更新日期：1996-10-21 00:00:00
abstract：:Previous work has shown that bilateral injection of as little as 10 microgram of cycloheximide (CHX) into the amygdala, but not into the internal capsule, caused a time-dependent disruption of long-term retention of passive avoidance training. Under these conditions, protein synthesis in the entire brain was inhibited...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(81)91178-1
更新日期：1981-03-23 00:00:00
abstract：:The possible involvement of the nitric oxide (NO)-cyclic GMP (cGMP)-protein kinase G (PKG) pathway on bovine lactoferrin (BLF)-induced spinal antihyperalgesic activity was elucidated in sciatic nerve injured rats. Intrathecal BLF reduced thermal hyperalgesia in a dose-dependent manner. Pretreatment with NG-L-nitro-arg...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.03.004
更新日期：2008-05-13 00:00:00
abstract：:Previous studies have indicated that central sensitization is a state of increased excitability of nociceptive neurons in the spinal dorsal horn following peripheral tissue injury and/or inflammation and astrocytes play an important role in the central sensitization. The current study investigated the role of amino ac...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2012.02.018
更新日期：2012-04-17 00:00:00
abstract：:We studied whether, similarly to the auditory modality, short-period temporal integration processes occur in vision. Event-related potentials (ERP) were recorded for occasional stimulus omissions from sequences of patterned visual stimuli. A posterior negative component emerged only when the constant stimulus onset as...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.05.034
更新日期：2006-08-09 00:00:00
abstract：:The immunocytochemical localization of the enzyme choline acetyltransferase (ChAT) was examined in the guinea pig organ of Corti to determine if both lateral and medial systems of efferents would show immunoreactive labeling for this specific enzyme marker of cholinergic neurons. Cochleae were also examined after lesi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90242-2
更新日期：1985-07-08 00:00:00
abstract：:Ion channels are integral membrane proteins that allow passive diffusion of ions across membranes. In neurons and in other excitable cells, the harmonious coordination between the numerous types of ion channels shape and propagate electrical signals. Increased accumulation of reactive oxidative species (ROS), and subs...
journal_title：Brain research
pub_type： 杂志文章,评审
doi：10.1016/j.brainres.2016.02.046
更新日期：2016-05-15 00:00:00
abstract：:The purpose of this study was to identify the functional fields activated in relation to the self-paced proximal and distal arm movements. The regional cerebral blood flow (rCBF) was measured with positron emission tomography (PET) and 15O-labelled H2O (H2(15)O) in eight healthy subjects. All subjects performed the fo...
journal_title：Brain research
pub_type： 临床试验,杂志文章
doi：10.1016/0006-8993(96)00032-7
更新日期：1996-04-15 00:00:00
abstract：:We sought to characterize regional heterogeneity of astrocytes using adrenergic receptor sites as cellular markers. Primary cultures made from 6 regions of neonatal rat brain consisted almost exclusively of astrocytes. Membranes from astrocytes cultured 1-3 weeks were prepared for radioligand binding assays of beta- a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91027-e
更新日期：1990-05-28 00:00:00
abstract：:To examine the role played by the basal forebrain cholinergic system in cortical activation, neuronal activity was investigated in the globus pallidus and substantia innominata of urethane-anaesthetized rats during large cortical slow waves and spontaneous or elicited low voltage fast activity. An effort was made to i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91521-6
更新日期：1987-12-22 00:00:00
abstract：:Our recent researches have identified increased expression of miR-21-5p in rats brain following traumatic brain injury (TBI), which protected against blood-brain barrier (BBB) damage. To further study the mechanism underlying the role of miR-21-5p on alleviating BBB damage after TBI, we performed the scratch injury mo...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.07.015
更新日期：2016-11-01 00:00:00
abstract：:Initial studies found glutamate injury to murine spinal cultures (14-17 days in vitro) to reflect contributions of both NMDA and AMPA/kainate receptors. Subsequent experiments found the spinal cultures to be more sensitive than cortical cultures to injury from prolonged low level kainate exposures, and, unlike cortica...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00532-u
更新日期：1995-08-21 00:00:00
abstract：:The properties of inward-rectifying membrane currents in immature rat olfactory cortex neurones (postnatal day (P) 10-22) were analysed using whole-cell patch-clamp recordings. In 78% of cells (40/51), injection of hyperpolarizing current pulses elicited graded electrotonic potentials showing a slowly developing sag i...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)01067-1
更新日期：1998-12-14 00:00:00
abstract：:Amphetamine releases dopamine through a transporter-mediated mechanism. The purpose of this report was to further our understanding of the intracellular pool from which amphetamine releases dopamine: the cytoplasmic pool, the vesicular pool, or both. Rats were treated with D-amphetamine (AMPH) (1.0 or 10.0 mg/kg) or a...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(98)00720-3
更新日期：1998-09-21 00:00:00
abstract：:The cloned 5-HT3 receptor from NCB-20 neuroblastoma cells was expressed in Xenopus oocytes. In these oocytes, 5-HT, the selective 5-HT3 receptor agonists, 2-methyl-5-HT and m-chlorophenylbiguanide activated an inward current which was sensitive to the specific 5-HT3 receptor antagonist LY278584. Cocaine (0.1 to 10 mic...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(94)01316-a
更新日期：1995-03-06 00:00:00
abstract：:Previous studies found that injection of the GABA uptake inhibitor nipecotic acid into the nucleus tractus solitarius (NTS) increases arterial pressure. This effect of nipecotic acid was not antagonized by the selective GABAA receptor blocking agent bicuculline, suggesting that the action of nipecotic acid was mediate...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91227-8
更新日期：1990-09-03 00:00:00
abstract：:The role of mu opioid receptors in the nucleus raphe dorsalis (DR) in the control of apomorphine-induced aggression was studied in rats. Administration in the DR of a selective mu opioid receptor agonist, (D-Ala2,N-Me-Phe4,Gly5-ol)-enkephalin (DAGO), in doses ranging from 0.01 to 1 microgram/0.5 microliter, dose-depen...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90706-3
更新日期：1989-05-29 00:00:00
