当前位置： SCI文献检索 > EUROPEAN JOURNAL OF HAEMATOLOGY期刊下所有文献 > Peripheral blood accessory cells modulate committed colony-forming units but not 5-week cobblestone-area-forming cell outgrowth from CD34+ cells.

Peripheral blood accessory cells modulate committed colony-forming units but not 5-week cobblestone-area-forming cell outgrowth from CD34+ cells.

Abstract：

:While cellular modulation in vitro of committed hematopoietic stem cell (HSC) growth has been known for some time, less is known about the effect of accessory cells (AC) on the growth of more immature HSC. We have examined the effect of peripheral blood (PB) AC on hematopoiesis by coculturing enriched PB CD34+ cells (>96% pure) with different quantities of CD34 cells (<1% contamination) harvested from 10 breast cancer patients. As expected colony growth was predominantly present in the CD34+ fractions, in which colony forming units granulocyte-macrophage (CFU-GM) varied between 89-3289/10(5) (median 1422/10(5) seeded cells) and week 5 cobblestone area forming cells (CAFC) between 64-1330/10(5) (median 427/10(5) seeded cells). Few CFU-GM (0-27/10(5) seeded cells) and no week 5 CAFC (0-1/10(5) seeded cells) were present in the CD34 fractions. The addition of PB CD34 cells to cultures of CD34+ cells resulted in a considerable variation in the cloning efficiency at the CFU-GM level, and the extent of modulation within the single patient was inconsistent between the different CD34+/CD34 cell mixtures. Overall the stimulatory effect was more pronounced than inhibition and on average the CFU-GM formation per CD34+ cell seeded increased 3 fold (stimulatory effect ranged between 3-17 fold and decreases between 2-10 fold). In contrast, the cloning efficiency at the week 5 CAFC level of differentiation remained unaffected by the addition of different amounts of CD34 cells (the stimulatory effect was maximally 3-fold and inhibition 3-fold). We conclude that while the CFU assay is modulated by the presence of AC, the CAFC assay is more robust and can be employed as a reliable and reproducible tool for HSC measurement.

journal_name

Eur J Haematol

journal_title

European journal of haematology

authors

Olesen G,Tønder H,Palshof T,Hokland P

doi

10.1034/j.1600-0609.2002.01520.x

subject

Has Abstract

pub_date

2002-02-01 00:00:00

pages

65-72

issue

2

eissn

0902-4441

issn

1600-0609

journal_volume

68

pub_type

杂志文章

相关文献

EUROPEAN JOURNAL OF HAEMATOLOGY文献大全
  • Causes of hypereosinophilia in 100 consecutive patients.

    abstract：BACKGROUND:Hypereosinophilia (HE, persistent peripheral blood eosinophilia > 1.5 × 109 /L) and hypereosinophilic syndrome (HES, HE with end-organ damage) are classified as primary (due to a myeloid clone), secondary (due to a wide variety of reactive causes), or idiopathic. Diagnostic evaluation of eosinophilia is chal...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/ejh.13437

    authors： Moller D,Tan J,Gauiran DTV,Medvedev N,Hudoba M,Carruthers MN,Dehghan N,van den Berghe J,Bruyère H,Chen LYC

    更新日期：2020-09-01 00:00:00

  • Expression of tartrate-resistant acid phosphatase in B-CLL treated with phorbol ester or phorbol ester plus calcium ionophore.

    abstract：:Cells from 3 patients with B-chronic lymphocytic leukaemia (B-CLL) and 1 with B-prolymphocytic leukaemia (B-PLL) were treated in vitro with the phorbol ester 12-0-tetradecanoylphorbol (TPA), the calcium ionophore A23187, or a combination of TPA and A23187. TPA induced the cells to adhere to the culture flask or to clu...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1600-0609.1988.tb01189.x

    authors： Drexler HG,Brenner MK,Gignac SM,Hoffbrand AV

    更新日期：1988-09-01 00:00:00

  • External validation and clinical evaluation of the International Prognostic Score of Thrombosis for Essential Thrombocythemia (IPSET-thrombosis) in a large cohort of Chinese patients.

    abstract：OBJECTIVES:In patients with essential thrombocythemia (ET), vascular complications contribute to both morbidity and mortality. To better predict the occurrence of thrombotic events, an International Prognostic Score of thrombosis for ET (IPSET-thrombosis) was recently developed. We hereby presented an external validati...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/ejh.12275

    authors： Fu R,Xuan M,Lv C,Zhang L,Li H,Zhang X,Zhang D,Sun T,Xue F,Liu X,Liang H,Zhang L,Yang R

    更新日期：2014-06-01 00:00:00

  • Congenital haemolytic anaemia in a low birth weight infant due to congenital stomatocytosis.

    abstract：:A baby girl born at 31 weeks gestation showed severe haemolytic anaemia and hyperbilirubinaemia which led to exchange transfusion within the first 12 hours of life. There was no blood group incompatibility between mother and child but there was a marked stomatocytosis of the baby's red blood cells. Family history reve...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1600-0609.1991.tb00554.x

    authors： Huppi PS,Ott P,Amato M,Schneider H

    更新日期：1991-07-01 00:00:00

  • Direct-acting oral anticoagulants: pharmacology, indications, management, and future perspectives.

    abstract：:In recent years, several direct-acting oral anticoagulants (DOAC) have become available for use in Europe and other regions in indications related to prophylaxis and treatment of venous and arterial thromboembolism. They include the oral direct thrombin inhibitor dabigatran etexilate (Pradaxa, Boehringer Ingelheim) an...

    journal_title：European journal of haematology

    pub_type： 杂志文章,评审

    doi：10.1111/ejh.12610

    authors： Gómez-Outes A,Suárez-Gea ML,Lecumberri R,Terleira-Fernández AI,Vargas-Castrillón E

    更新日期：2015-11-01 00:00:00

  • Allogeneic hematopoietic cell transplantation for paroxysmal nocturnal hemoglobinuria.

    abstract：BACKGROUND:Although allogeneic hematopoietic cell transplantation (HCT) has a potential to cure patients with paroxysmal nocturnal hemoglobinuria (PNH), appropriate indication and conditioning regimen for HCT have not been established. PATIENTS AND METHODS:Between July 1999 and December 2001, five patients with PNH un...

    journal_title：European journal of haematology

    pub_type： 临床试验,杂志文章

    doi：10.1034/j.1600-0609.2003.00097.x

    authors： Lee JL,Lee JH,Lee JH,Choi SJ,Kim S,Seol M,Lee YS,Chi HS,Park CJ,Kim WK,Lee JS,Lee KH

    更新日期：2003-08-01 00:00:00

  • An Arg545----Cys545 substitution mutation of the von Willebrand factor in type IIB von Willebrand's disease.

    abstract：:Type IIB is a special variant of von Willebrand's disease, characterized by an abnormal von Willebrand factor which shows an increased interaction with platelets. This interaction sometimes causes platelet aggregation and thrombocytopenia in vivo. It involves the glycoprotein-Ib (GPIb) receptor on platelets and corres...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1600-0609.1991.tb01858.x

    authors： Donnér M,Andersson AM,Kristoffersson AC,Nilsson IM,Dahlbäck B,Holmberg L

    更新日期：1991-11-01 00:00:00

  • A comparison of fluorine-18 fluoro-deoxyglucose PET and technetium-99m sestamibi in assessing patients with multiple myeloma.

    abstract：OBJECTIVE:The extent of disease in patients with multiple myeloma or related conditions may be difficult to assess. In previous small studies, both FDG-PET (PET) and Tc-99m sestamibi scans (MIBI) have identified sites of occult disease in myeloma. METHODS:We reviewed the results for patients at our institution who hav...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.0902-4441.2004.00177.x

    authors： Mileshkin L,Blum R,Seymour JF,Patrikeos A,Hicks RJ,Prince HM

    更新日期：2004-01-01 00:00:00

  • Hematologic and molecular spontaneous remission following sepsis in acute monoblastic leukemia with translocation (9;11): a case report and review of the literature.

    abstract：:Spontaneous remission in patients with acute myeloid leukemia (AML) is a rarely reported phenomenon of usually short duration. The etiology remains unclear, but an association with preceding blood transfusions or bacterial infections has been reported. Triggered immune responses are suggested to play a potential role ...

    journal_title：European journal of haematology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1600-0609.2004.00248.x

    authors： Müller CI,Trepel M,Kunzmann R,Lais A,Engelhardt R,Lübbert M

    更新日期：2004-07-01 00:00:00

  • Engraftment of human myelodysplastic syndrome derived cell line in transgenic severe combined immunodeficient (TG-SCID) mice expressing human GM-CSF and IL-3.

    abstract：:A transgenic SCID (TG-SCID) mouse expressing the human cytokines interleukin-3 (IL-3) and granulocyte-macrophage colony-stimulating factor (GM-CSF) has been generated with the aim of making a model system allowing the in vivo proliferation of human hematopoietic cells. Using TG-SCID mice expressing high levels (30-35 ...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1600-0609.1998.tb01067.x

    authors： Kim DK,Kojima M,Fukushima T,Miyasaka M,Nakauchi H

    更新日期：1998-08-01 00:00:00

  • Identification and characterization of the first large deletion of the MYH9 gene associated with MYH9 disorders.

    abstract：:MYH9 disorders are autosomal dominant macrothrombocytopenias with leukocyte inclusion bodies. Single point mutations in the protein-coding sequence of the MYH9 gene are the most common cause. So far no large gene deletion/insertion and splicing defects have been reported. Conventional DNA sequencing of each MYH9-codin...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1600-0609.2008.01046.x

    authors： Kunishima S,Matsushita T,Hamaguchi M,Saito H

    更新日期：2008-06-01 00:00:00

  • Homoharringtonine affects the JAK2-STAT5 signal pathway through alteration of protein tyrosine kinase phosphorylation in acute myeloid leukemia cells.

    abstract：OBJECTIVES:Homoharringtonine (HHT) was efficient in therapying patients with acute myeloid leukemia (AML) in China, but little is known about the mechanism of its action. As the abnormal activation of JAK2 associated pathway is important to AML, we try to explore the effect of HHT on JAK2-STAT pathway in AML cells, thu...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1600-0609.2008.01116.x

    authors： Tong H,Ren Y,Zhang F,Jin J

    更新日期：2008-10-01 00:00:00

  • Characterization of a new human plasma cell leukemia cell line UHKT-944.

    abstract：OBJECTIVE:A new interleukin-6 (IL-6)-dependent plasma cell leukemia cell line UHKT-944 was established from bone marrow cells derived from a 55-yr-old man with plasma cell leukemia. RESULTS:The cell line possesses phenotypic characteristics of plasma cells including the production of a monoclonal immunoglobulin IgA1-k...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/ejh.12505

    authors： Vyhlídalová I,Uherková L,Pleschnerová M,Špička I,Březinová J,Michalová K,Čermáková K,Polanská V,Jedelský PL,Hamšíková E,Kuželová K,Stöckbauer P

    更新日期：2015-10-01 00:00:00

  • The lipocalin 24p3, which is an essential molecule in IL-3 withdrawal-induced apoptosis, is not involved in the G-CSF withdrawal-induced apoptosis.

    abstract：:Many hematopoietic cells undergo apoptosis when deprived of specific cytokines. Lipocalin 24p3, reported to be induced in hematopoietic cells by interleukin 3 (IL-3) depletion, induces hematopoietic cell apoptosis despite the presence of IL-3. As granulocyte colony stimulating factor (G-CSF) depletion also induces the...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.0902-4441.2003.00160.x

    authors： Kamezaki K,Shimoda K,Numata A,Aoki K,Kato K,Takase K,Nakajima H,Ihara K,Haro T,Ishikawa F,Imamura R,Miyamoto T,Nagafuji K,Gondo H,Hara T,Harada M

    更新日期：2003-12-01 00:00:00

  • Factors influencing release of granulocyte-macrophage colony-stimulating activity from human mononuclear phagocytes.

    abstract：:Mononuclear phagocytes play an important role in the regulation of hematopoiesis, not only by producing regulatory monokines such as prostaglandins, tumor necrosis factor and interleukin-1 (IL-1), but also by the production of colony-stimulating activity (CSA). Previously, we have demonstrated that granulocyte-macroph...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1600-0609.1988.tb00209.x

    authors： Fibbe WE,Kluck PM,Duinkerken N,Voogt PJ,Willemze R,Falkenburg JH

    更新日期：1988-10-01 00:00:00

  • Novel G6B gene variant causes familial autosomal recessive thrombocytopenia and anemia.

    abstract：OBJECTIVE:To characterize the underlying genetic and molecular defects in a consanguineous family with lifelong blood disorder manifested with thrombocytopenia (low platelets count) and anemia. METHODS:Genetic linkage analysis, exome sequencing, and functional genomics were carried out to identify and characterize the...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/ejh.12819

    authors： Melhem M,Abu-Farha M,Antony D,Madhoun AA,Bacchelli C,Alkayal F,AlKhairi I,John S,Alomari M,Beales PL,Alsmadi O

    更新日期：2017-03-01 00:00:00

  • BCL3 rearrangement, amplification and expression in diffuse large B-cell lymphoma.

    abstract：AIMS:Aim of the study is to investigate diffuse large B-cell lymphoma (DLBCL) for the presence of BCL3 gene rearrangement and protein expression and to correlate these with immunophenotypic subsets of DLBCL. We aimed to investigate the pathogenetic implication of BCL3 in DLBCL. METHODS AND RESULTS:Tissue microarray se...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1600-0609.2011.01684.x

    authors： Ibrahim HA,Amen F,Reid AG,Naresh KN

    更新日期：2011-12-01 00:00:00

  • HIV+ MALT lymphoma remission induced by highly active antiretroviral therapy alone.

    abstract：:MALT lymphoma is usually described in association with Helicobacter pylori, HCV, HHV8, Campylobacter jejuni or in a setting of overreactive immunity. In HIV(+) patients, MALT lymphoma is most commonly described in children. We describe here an original case of HIV(+) MALT lymphoma with bronchial, conjuctival and laryn...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1600-0609.2004.00339.x

    authors： Girard T,Luquet-Besson I,Baran-Marszak F,Raphaël M,Boué F

    更新日期：2005-01-01 00:00:00

  • A fatal case of enoxaparin induced skin necrosis and thrombophilia.

    abstract：:Skin necrosis caused by heparins is a rare complication. We report a case of a 71-yr-old white woman who developed painful diffuse skin lesions, most probably related to enoxaparin treatment. Other causes of skin necrosis, including heparin induced thrombocytopenia, disseminated intravascular coagulation, protein C/pr...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1600-0609.2006.00668.x

    authors： Nadir Y,Mazor Y,Reuven B,Sarig G,Brenner B,Krivoy N

    更新日期：2006-08-01 00:00:00

  • Assessment of the value of treatment with granulocyte colony-stimulating factor in children with acute lymphoblastic leukemia: a randomized clinical trial.

    abstract：:The present trial was designed to test the effects of G-CSF on the duration of the second phase of induction chemotherapy in children with newly diagnosed acute lymphoblastic leukemia (ALL). A total of 32 patients were assigned randomly to a group that received (14 patients; group A) or a group that did not receive (1...

    journal_title：European journal of haematology

    pub_type： 临床试验,杂志文章,随机对照试验

    doi：10.1111/j.1600-0609.1995.tb01816.x

    authors： Dibenedetto SP,Ragusa R,Ippolito AM,Lo Nigro L,Di Cataldo A,D'Amico S,Miraglia V

    更新日期：1995-08-01 00:00:00

  • Comparison of poly- and monoclonal antibodies for determination of B-cell clonal excess in an routine clinical laboratory.

    abstract：:Flow cytometry (FCM) has gained wide use in the determination of clonality in B-cell lymphoproliferative diseases and many methodological variations exist. We have compared the suitability of a) dual fluorochrome (FITC/PE)-labelled monoclonal antibodies, b) single fluorochrome (FITC)-labelled monoclonal antibodies and...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1600-0609.1992.tb01793.x

    authors： Agrawal YP,Hämäläinen E,Mahlamäki EK,Aho H,Nousianinen T,Lahtinen R,Penttilä IM

    更新日期：1992-01-01 00:00:00

  • Primary osteomyelofibrosis and an XX-male genotype.

    abstract：:A 62-yr-old man with two healthy daughters was diagnosed with osteomyelofibrosis. To our surprise, a female XX-karyotype was observed in bone marrow and confirmed in PHA-stimulated T-lymphocytes from peripheral blood. Further molecular genetic investigation revealed a submicroscopic translocation between the short arm...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/ejh.12555

    authors： Schanz J,Haase D,Steuernagel P,Shirneshan K,Bäsecke J

    更新日期：2015-09-01 00:00:00

  • Cost-effectiveness analysis of interventions for prevention of invasive aspergillosis among leukemia patients during hospital construction activities.

    abstract：OBJECTIVES:Invasive Aspergillosis (IA) is a serious problem among hematological patients and it is associated with high mortality. This situation can worsen at times of hospital construction, however there are several preventive measures available. This work aims to define the cost-effectiveness of some of these interv...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/ejh.12991

    authors： Combariza JF,Toro LF,Orozco JJ,Arango M

    更新日期：2018-02-01 00:00:00

  • Differential expression of CD25 and HC2 antigens on subtypes of acute myeloid leukemias.

    abstract：:The presence of CD25 and HC2 antigens in 66 different patients with acute myeloid leukemia (AML) was investigated. The expression of both antigens was observed in 32% of AML cells. Dual fluorescence staining experiments performed in 5 AML patient cells showed that CD25 and HC2 antigens were simultaneously expressed in...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1600-0609.1989.tb01467.x

    authors： Padrós MR,Salamone MC,Zunszain PA,Fainboim L

    更新日期：1989-05-01 00:00:00

  • Whole blood folate values in pernicious anaemia: relation to treatment.

    abstract：:27 patients with pernicious anaemia, followed for a long period, were consecutively treated with three different vitamin B12 preparations, while during intervening period no therapy was given until signs of B12 deficiency developed. After vitamin B12 treatment, a peak whole blood folate value some 70% higher than the ...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1600-0609.1987.tb00161.x

    authors： Magnus EM

    更新日期：1987-07-01 00:00:00

  • BAALC and WT1 expressions from diagnosis to hematopoietic stem cell transplantation: consecutive monitoring in adult patients with core-binding-factor-positive AML.

    abstract：:No consecutive analysis of BAALC and WT1 expressions associated with core-binding factor AML (CBF-AML) from diagnosis to hematopoietic stem cell transplantation (HSCT) has yet been reported. We investigated BAALC and WT1 expressions using a method of real-time quantitative polymerase chain reaction (RQ-PCR) at diagnos...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/ejh.12142

    authors： Yoon JH,Kim HJ,Shin SH,Yahng SA,Lee SE,Cho BS,Eom KS,Kim YJ,Lee S,Min CK,Cho SG,Kim DW,Lee JW,Min WS,Park CW,Lim JH

    更新日期：2013-08-01 00:00:00

  • Anagrelide influences thrombotic risk, and prolongs progression-free and overall survival in essential thrombocythaemia vs hydroxyurea plus aspirin.

    abstract：OBJECTIVE:We report an extension study of patients with essential thrombocythaemia (ET) in the Hungarian Myeloproliferative Neoplasm (HUMYPRON) Registry, which demonstrated that over 6 years anagrelide significantly decreased the number of patients experiencing minor arterial and minor venous thrombotic events (TEs) vs...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/ejh.13459

    authors： Kellner A,Dombi P,Illes A,Demeter J,Homor L,Ercsei I,Simon Z,Karadi E,Herczeg J,Gy Korom V,Gasztonyi Z,Szerafin L,Udvardy M,Egyed M

    更新日期：2020-10-01 00:00:00

  • Prevalence and risk factors for Pulmonary Hypertension associated with chronic Myeloproliferative Neoplasms.

    abstract：OBJECTIVES:Pulmonary hypertension (PH) is commonly reported in Philadelphia-chromosome negative myeloproliferative neoplasms (MPNs) including polycythemia vera (PV), essential thrombocythemia (ET), and myelofibrosis (MF). PH may be diagnosed directly by right heart catheterization (RHC) or estimated by transthoracic ec...

    journal_title：European journal of haematology

    pub_type： 杂志文章

    doi：10.1111/ejh.13543

    authors： Ferrari A,Scandura J,Masciulli A,Krichevsky S,Gavazzi A,Barbui T

    更新日期：2021-02-01 00:00:00

  • The molecular pathogenesis of the myelodysplastic syndromes.

    abstract：:Recent studies have greatly illuminated the genomic landscape of the myelodysplastic syndromes (MDS), and the pace of discovery is accelerating. The most common mutations found in MDS occur in genes involved in RNA splicing (including SF3B1, SRSF2, U2AF1, and ZRSR2) and epigenetic modification (including TET2, ASXL1, ...

    journal_title：European journal of haematology

    pub_type： 杂志文章,评审

    doi：10.1111/ejh.12515

    authors： Pellagatti A,Boultwood J

    更新日期：2015-07-01 00:00:00

  • Autoimmune hemolytic anemia associated with the use of immune checkpoint inhibitors for cancer: 68 cases from the Food and Drug Administration database and review.

    abstract：BACKGROUND:Immune checkpoint inhibitors (CPI) are widely used in modern oncology and have improved the prognosis of lung cancer, malignant melanoma, and other malignancies. Unlike cytotoxic chemotherapy, drugs such as nivolumab, pembrolizumab, and ipilimumab are associated with immune-related adverse effects. We recent...

    journal_title：European journal of haematology

    pub_type： 杂志文章,meta分析,评审

    doi：10.1111/ejh.13187

    authors： Tanios GE,Doley PB,Munker R

    更新日期：2019-02-01 00:00:00