当前位置： SCI文献检索 > BRITISH JOURNAL OF HAEMATOLOGY期刊下所有文献 > Differentiation of functional dendritic cells and macrophages from human peripheral blood monocyte precursors is dependent on expression of p21 (WAF1/CIP1) and requires iron.

Differentiation of functional dendritic cells and macrophages from human peripheral blood monocyte precursors is dependent on expression of p21 (WAF1/CIP1) and requires iron.

Abstract：

:Iron is required for monocyte/macrophage differentiation of HL-60 leukaemia cells. Differentiation requires induction of the cyclin-dependent kinase inhibitor p21 (WAF1/CIP1), and cell cycle arrest at the G1/S checkpoint. With iron depletion, p21 induction and differentiation are blocked. To establish the roles of iron and p21 in normal monocyte/macrophage differentiation, we examined generation of dendritic cells (DCs) and macrophages from peripheral monocytes. Monocytes were cultured with interleukin 4 and granulocyte-macrophage colony-stimulating factor (GM-CSF), then treated with lipopolysaccharide to produce DCs or with M-CSF to produce macrophages. Iron deprivation was induced by desferrioxamine (DF). Monocyte-derived DCs had characteristic phenotype and morphology, and stimulated proliferation of naïve allogeneic T lymphocytes. In contrast, DCs generated under iron deprivation were phenotypically undifferentiated and did not stimulate T cells. Similarly, macrophages expressed a characteristic phenotype and morphology, and phagocytosed latex beads, but macrophages generated under iron deprivation failed to develop a mature phenotype and had impaired phagocytosis. Iron deprivation blocked induction of p21 (WAF1/CIP1) expression in both DC and macrophage cultures. Furthermore, p21 antisense oligonucleotides, but not sense oligonucleotides, inhibited both DC and macrophage differentiation. These data indicate that a key role of iron in haematopoiesis is to support induction of p21 which, in turn, is required for DC and macrophage differentiation.

journal_name

Br J Haematol

journal_title

British journal of haematology

authors

Kramer JL,Baltathakis I,Alcantara OS,Boldt DH

doi

10.1046/j.1365-2141.2002.03498.x

subject

Has Abstract

pub_date

2002-06-01 00:00:00

pages

727-34

issue

3

eissn

0007-1048

issn

1365-2141

pii

3498

journal_volume

117

pub_type

杂志文章

相关文献

BRITISH JOURNAL OF HAEMATOLOGY文献大全
  • MDS-type abnormalities within myeloma signature karyotype (MM-MDS): only 13% 1-year survival despite tandem transplants.

    abstract：:Cytogenetic abnormalities (CA), especially of chromosome 13, have been used to identify a subgroup of previously untreated multiple myeloma (MM) patients with very poor prognosis despite high-dose therapy (HDT). We examined the prognostic implications of CA in 1000 MM patients receiving melphalan-based tandem autotran...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2141.2003.04455.x

    authors： Jacobson J,Barlogie B,Shaughnessy J,Drach J,Tricot G,Fassas A,Zangari M,Giroux D,Crowley J,Hough A,Sawyer J

    更新日期：2003-08-01 00:00:00

  • Prevalence of anti-FVIII antibodies in severe haemophilia A patients with inversion of intron 22.

    abstract：:The aim of our study was to investigate whether haemophilia A patients with inversion of intron 22 are at high risk for non-inhibitory anti-FVIII antibodies development detected by ELISA. It is known that patients with severe forms of haemophilia A are more likely to develop anti-FVIII antibodies. The incidence of inh...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2141.1997.1082922.x

    authors： Vianello F,Radossi P,Tison T,Dazzi F,Tagariello G,Davoli PG,Girolami A

    更新日期：1997-06-01 00:00:00

  • Identification of a tissue kallikrein in human polymorphonuclear leucocytes.

    abstract：:We have identified a tissue kallikrein in polymorphonuclear (PMN) leucocytes of normal human blood and bone marrow by immunocytochemistry, radioimmunoassay and enzymology. Immunoreactive tissue kallikrein was visualized in the mature neutrophil leucocytes and in immature forms such as metamyelocytes and myelocytes. No...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1989.tb07711.x

    authors： Figueroa CD,MacIver AG,Bhoola KD

    更新日期：1989-07-01 00:00:00

  • Clinical significance of Aspergillus fungaemia in patients with haematological malignancies and invasive aspergillosis.

    abstract：:The clinical significance of Aspergillus fungaemia in the setting of a deep-seated aspergillosis has not been clearly established. Among 107 microbiologically documented Aspergillus infections in patients with haematological diseases observed over a 17-year period, blood cultures grew Aspergillus species from 10 cases...

    journal_title：British journal of haematology

    pub_type： 杂志文章,评审

    doi：10.1046/j.1365-2141.2001.02901.x

    authors： Girmenia C,Nucci M,Martino P

    更新日期：2001-07-01 00:00:00

  • Impact of unrelated donor status on the incidence and outcome of cytomegalovirus infections after non-myeloablative allogeneic stem cell transplantation.

    abstract：:Little is known about the impact of cytomegalovirus (CMV) infections that occur after human leucocyte antigen (HLA)-matched unrelated donor (MUD) non-myleoablative haematopoietic stem cell transplantation (HCT). We analysed the incidence, onset and outcomes of CMV infections in 59 recipients of MUD and in 109 recipien...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2141.2003.04671.x

    authors： Junghanss C,Storb R,Maris MB,Carter RA,Sandmaier BM,Maloney DG,McSweeney PA,Corey L,Boeckh M

    更新日期：2003-11-01 00:00:00

  • CXCR4 mutation subtypes impact response and survival outcomes in patients with Waldenström macroglobulinaemia treated with ibrutinib.

    abstract：:Ibrutinib is associated with response rate of 90% and median progression-free survival (PFS) in excess of 5 years in Waldenström macroglobulinaemia (WM) patients. CXCR4 mutations are detected in 30-40% of patients with WM and associate with lower rates of response and shorter PFS to ibrutinib therapy. Both frameshift ...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/bjh.16088

    authors： Castillo JJ,Xu L,Gustine JN,Keezer A,Meid K,Dubeau TE,Liu X,Demos MG,Kofides A,Tsakmaklis N,Chen JG,Munshi M,Guerrera ML,Chan GG,Patterson CJ,Yang G,Hunter ZR,Treon SP

    更新日期：2019-11-01 00:00:00

  • A role for complement as the major opsonin in the sequestration of erythrocytes from elderly and young donors.

    abstract：:Erythrocytes from elderly donors (> 70 years), but not young donors (18-35 years), are shown to undergo sequestration in an in vitro erythrophagocytosis assay. Comparable levels of sequestration are observed for high density erythrocytes from young individuals and both low density and high density erythrocytes from el...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1993.tb04705.x

    authors： Shapiro S,Kohn D,Gershon H

    更新日期：1993-04-01 00:00:00

  • Absence of N-RAS point mutations in peripheral blood cells of patients with aplastic anaemia and paroxysmal nocturnal haemoglobinurea.

    abstract：:The myelodysplastic syndromes (MDS) have a significant frequency of evolution into acute myeloid leukaemia (AML). Approximately 30% of MDS patients show activating mutations of the N-RAS proto-oncogene, and these patients are at increased risk of leukaemic evolution. Long-term survivors of aplastic anaemia (AA) and pa...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1995.tb05411.x

    authors： White JR,Josten KM,Chopra R,Tooze J,Saso R,Gordon-Smith EC,Rutherford TR

    更新日期：1995-12-01 00:00:00

  • A new type of hereditary persistence of fetal haemoglobin (HPFH): HPFH Tunisia beta + (+C-200)G gamma.

    abstract：:Nd-HPFH are haematological conditions which are natural models to aid understanding of the haemoglobin (Hb) switch. In this paper we describe a new non-deletional hereditary persistence of fetal haemoglobin (nd-HPFH) associated with the highest Hb F level observed to date (up to 49% without haemopoietic stress). Seque...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：

    authors： Pissard S,M'rad A,Beuzard Y,Roméo PH

    更新日期：1996-10-01 00:00:00

  • Comparative outcomes of immunochemotherapy regimens in Waldenström macroglobulinaemia.

    abstract：:Comparative data on immunochemotherapy regimens for Waldenström macroglobulinaemia/lymphoplasmacytic lymphoma (WM/LPL) are lacking. We analysed overall survival (OS), risk of hospitalizations, transfusions and plasmapheresis in a population-based cohort of patients ≥65 years old initiating WM/LPL therapy in 1999-2013....

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/bjh.14828

    authors： Olszewski AJ,Chen C,Gutman R,Treon SP,Castillo JJ

    更新日期：2017-10-01 00:00:00

  • Pathogenesis of hepatic fibrosis in experimental iron overload.

    abstract：:Significant increases in prolyl hydroxylase activity, a key enzyme in the collagen biosynthetic pathway, were noted in the hepatic homogenates of iron-loaded animals as compared to controls. The increase in prolyl hydroxylase activity was seen without any light microscopic histologic evidence of cell necrosis or accum...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1985.tb02997.x

    authors： Weintraub LR,Goral A,Grasso J,Franzblau C,Sullivan A,Sullivan S

    更新日期：1985-02-01 00:00:00

  • A novel 7.9 kb deletion causing alpha+-thalassaemia in two independent families of Indian origin.

    abstract：:We describe the characterization of a novel 7.9 kb deletion that eliminated one of the duplicated alpha-globin genes, causing an alpha+-thalassaemia phenotype in two independent carriers of Suriname-Indian origin. The molecular characterization of the deletion breakpoint fragment revealed neither involvement of Alu re...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2141.2003.04060.x

    authors： Harteveld CL,van Delft P,Wijermans PW,Kappers-Klunne MC,Weegenaar J,Losekoot M,Giordano PC

    更新日期：2003-01-01 00:00:00

  • Treatment and prognosis of mature (non-anaplastic) T- and NK-cell lymphomas in childhood, adolescents, and young adults.

    abstract：:Paediatric non-Hodgkin lymphomas (pNHL) are a diverse group of malignancies characterised by nodal and/or extranodal involvement. Less common pNHL forms include those derived from mature T- and natural killer (NK) cells. Much of our current understanding of paediatric mature (non-anaplastic) T/NK-cell lymphomas with r...

    journal_title：British journal of haematology

    pub_type： 杂志文章,评审

    doi：10.1111/bjh.15772

    authors： Xavier AC,Suzuki R

    更新日期：2019-06-01 00:00:00

  • Molecular characterization of a normal Hb A2 beta-thalassaemia determinant in a Sardinian family.

    abstract：:In this study we have carried out haplotype analysis at the beta-globin gene cluster and defined the beta-thalassaemia mutations in a large Sardinian family, ascertained through a proband with thalassaemia major, in which several members were carriers of a beta-thalassaemia allele characterized by microcytosis, hypoch...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1987.tb02331.x

    authors： Oggiano L,Pirastu M,Moi P,Longinotti M,Perseu L,Cao A

    更新日期：1987-10-01 00:00:00

  • Human herpesvirus 8 DNA sequences are present in bone marrow from HIV-negative patients with lymphoproliferative disorders and from healthy donors.

    abstract：:Bone marrow (BM) from patients affected by multiple myeloma (MM), exhibiting monoclonal gammopathy of undetermined significance (MGUS) or with non-Hodgkin lymphoma (NHL) as well as from healthy donors were investigated for the presence of human herpesvirus-8 (HHV-8) DNA sequences. ORF 26 sequences were detected in 36-...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2141.2001.02702.x

    authors： Azzi A,Fanci R,De Santis R,Ciappi S,Paci C

    更新日期：2001-04-01 00:00:00

  • Residual expression of functional MHC class II molecules in twin brothers with MHC class II deficiency is cell type specific.

    abstract：:We examined major histocompatibility complex (MHC) class II expression in B cells, peripheral blood monocytes, activated T cells, epidermal Langerhans cells, monocyte-derived dendritic cells, dermal microvascular endothelial cells (DMEC) and fibroblasts of twin brothers with MHC class II deficiency. Although residual ...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2141.2001.03105.x

    authors： Wolf HM,Thon V,Gulle H,Lechleitner S,Eibl MM,Petzelbauer P

    更新日期：2001-11-01 00:00:00

  • Resistance to proteasome inhibitors and other targeted therapies in myeloma.

    abstract：:The number of novel therapies for the treatment of myeloma is rapidly increasing, as are the clinical trials evaluating them in combination with other novel and established therapies. Proteasome inhibitors, immunomodulatory agents and monoclonal antibodies are the most well known and studied classes of novel agents ta...

    journal_title：British journal of haematology

    pub_type： 杂志文章,评审

    doi：10.1111/bjh.15210

    authors： Wallington-Beddoe CT,Sobieraj-Teague M,Kuss BJ,Pitson SM

    更新日期：2018-07-01 00:00:00

  • The cytoskeletal linker protein moesin: decreased levels in Wiskott-Aldrich syndrome platelets and identification of a cleavage pathway in normal platelets.

    abstract：:The Wiskott-Aldrich syndrome (WAS) is a severe disease of platelets (small size, thrombocytopenia) and lymphocytes (immunodeficiency) arising from mutations of the X-chromosome gene WASP. Because of the prominent role of cytoskeletal abnormalities, particularly the paucity of surface microvilli, in the cellular pathol...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2141.1999.01508.x

    authors： Shcherbina A,Bretscher A,Rosen FS,Kenney DM,Remold-O'Donnell E

    更新日期：1999-07-01 00:00:00

  • Gene silencing by DNA methylation in haematological malignancies.

    abstract：:The past decade has seen an explosion of interest in the epigenetics of cancer, with an increasing understanding that this form of genomic modification plays a critical role in pathogenesis. The malignant phenotype results from a step-wise increase of both genetic abnormalities and epigenetic modifications, leading to...

    journal_title：British journal of haematology

    pub_type： 杂志文章,评审

    doi：10.1111/j.1365-2141.2007.06604.x

    authors： Boultwood J,Wainscoat JS

    更新日期：2007-07-01 00:00:00

  • Assessment of complement binding by anti-D and anti-M antibodies employing labelled antiglobulin antibodies.

    abstract：:The presence of small amounts of C3d on freshly obtained normal red blood cells (RBC) was demonstrated by a radio-labelled antiglobulin technique; this increased 1.5-fold after incubation in fresh normal serum. No significant further increase in bound C3d was demonstrated for cDE/cDE, CDe/CDe, -D-/-D- RBC maximally se...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1980.tb07150.x

    authors： Freedman J,Massey A,Chaplin H,Monroe MC

    更新日期：1980-06-01 00:00:00

  • Combined defect in membrane expression and activation of platelet GPIIb--IIIa complex without primary sequence abnormalities in myeloproliferative disease.

    abstract：:Defects in glycoprotein (GP)IIb-IIIa or in its activation may cause abnormal platelet aggregation and a bleeding diathesis. We report studies in a 67-year-old man with a myeloproliferative disease and markedly abnormal platelet responses. By flow cytometry, platelet binding of two complex-specific anti-GPIIb-IIIa mono...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：

    authors： Kaplan R,Gabbeta J,Sun L,Mao GF,Rao AK

    更新日期：2000-12-01 00:00:00

  • In vitro differentiation of myeloid progenitor cells in patients with eosinophilia.

    abstract：:Colony-forming cells of the granulocytic/macrophage ( CFCGM ) and eosinophilic ( CFCEo ) series were grown from bone marrow and/or peripheral blood of 20 patients with persistent eosinophilia mainly due to helminthic diseases and of 17 patients without eosinophilia. The semi-solid culture technique of haemopoietic cel...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：

    authors： Kern P,Dietrich M

    更新日期：1984-05-01 00:00:00

  • Megakaryopoiesis in patients with cyclic thrombocytopenia.

    abstract：:Megakaryopoiesis was examined in 10 patients (eight females and two males) with cyclic thrombocytopenia (CT) to investigate the underlying pathogenesis. Numbers of CFU-Meg and megakaryocytes and the mean cytoplasmic area (mean area) of megakaryocytes at the peak, nadir, ascent mid phase, and descent mid phase of the p...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1995.tb05267.x

    authors： Nagasawa T,Hasegawa Y,Kamoshita M,Ohtani K,Komeno T,Itoh T,Shinagawa A,Kojima H,Ninomiya H,Abe T

    更新日期：1995-09-01 00:00:00

  • Successful treatment of transfusion-associated graft-versus-host disease.

    abstract：:We present an immunocompetent patient with transfusion-associated graft-versus-host disease (GVHD), in which chimaerism of peripheral blood lymphocytes was demonstrated by analysis of a highly polymorphic genome. The patient was treated successfully with anti-CD3 monoclonal antibody, OKT3 and cyclosporin A. Although i...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1994.tb04838.x

    authors： Yasukawa M,Shinozaki F,Hato T,Takada K,Ishii Y,Tamai T,Takamatsu H,Shiobara S,Yoshizaki K,Fujita S

    更新日期：1994-04-01 00:00:00

  • In vitro Ig-synthesis and proliferative activity in multiple myeloma are stimulated by different growth factors.

    abstract：:Plasma cells isolated from bone marrow (BM) aspirates of 15 patients with active multiple myeloma (MM) were cultured and analysed for in vitro proliferative response and Ig-synthesis upon stimulation with interleukin-3 (IL-3), interleukin-4 (IL-4) and interleukin-6 (IL-6). The proliferative response, determined as Ki-...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1991.tb08086.x

    authors： Sonneveld P,Schoester M,de Leeuw K

    更新日期：1991-12-01 00:00:00

  • Lymphoid subsets and prognostic factors in multiple myeloma. Cooperative Group for the Study of Monoclonal Gammopathies.

    abstract：:In a uniform series of 170 untreated myeloma patients (MM) we investigated the distribution of T cell subsets in peripheral blood (PB) and their relationship with the most relevant disease characteristics, including survival. CD4 cells were significantly decreased both in percentage and absolute numbers (P less than 0...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1992.tb08137.x

    authors： San Miguel JF,González M,Gascón A,Moro MJ,Hernández JM,Ortega F,Jiménez R,Guerras L,Romero M,Casanova F

    更新日期：1992-03-01 00:00:00

  • Simultaneous inheritance of mutant isoenzymes of erythrocyte pyruvate kinase associated with chronic haemolytic anaemia.

    abstract：:The heterogeneity of pyruvate kinase (PK) deficiency associated with hereditary haemolytic anaemia is emphasized by studies of a kindred harbouring two distinct mutant forms of this enzyme, both of which were kinetically defective with markedly decreased affinities for the substrate, phosphoenolypyruvate. The two isoe...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.1976.tb00174.x

    authors： Pagila DE,Gray GR,Growe GH,Valentine WN

    更新日期：1976-09-01 00:00:00

  • Risk factors for early death in neonates with Down syndrome and transient leukaemia.

    abstract：:Transient leukaemia (TL) in neonates with Down syndrome (DS) is characterized by the transient appearance of blast cells in the peripheral blood that resolves spontaneously. Some TL patients die at an early age due to organ failure. Seventy DS patients with TL were studied retrospectively to identify clinical and labo...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1111/j.1365-2141.2008.07231.x

    authors： Muramatsu H,Kato K,Watanabe N,Matsumoto K,Nakamura T,Horikoshi Y,Mimaya J,Suzuki C,Hayakawa M,Kojima S

    更新日期：2008-08-01 00:00:00

  • Selective enhancement of thrombopoietic activity of PEGylated interleukin 6 by a simple procedure using a reversible amino-protective reagent.

    abstract：:We developed a novel method for the chemical modification of cytokines with synthetic polymers to increase the therapeutic efficacy of the former in vivo. A pH-reversible amino-protective reagent, dimethylmaleic anhydride (DMMAn), was used for modification of interleukin-6 (IL-6) with polyethylene glycol (PEG). The no...

    journal_title：British journal of haematology

    pub_type： 杂志文章

    doi：10.1046/j.1365-2141.2001.02508.x

    authors： Tsunoda S,Ishikawa T,Watanabe M,Kamada H,Yamamoto Y,Tsutsumi Y,Hirano T,Mayumi T

    更新日期：2001-01-01 00:00:00

  • A multi-centre study of therapeutic efficacy and safety of platelet components treated with amotosalen and ultraviolet A pathogen inactivation stored for 6 or 7 d prior to transfusion.

    abstract：:Bacteria in platelet components (PC) may result in transfusion-related sepsis (TRS). Pathogen inactivation of PC with amotosalen (A-PC) can abrogate the risk of TRS and hence facilitate storage to 7 d. A randomized, controlled, double-blinded trial to evaluate the efficacy and safety of A-PC stored for 6-7 d was condu...

    journal_title：British journal of haematology

    pub_type： 杂志文章,多中心研究,随机对照试验

    doi：10.1111/j.1365-2141.2011.08635.x

    authors： Lozano M,Knutson F,Tardivel R,Cid J,Maymó RM,Löf H,Roddie H,Pelly J,Docherty A,Sherman C,Lin L,Propst M,Corash L,Prowse C

    更新日期：2011-05-01 00:00:00