Abstract:
:1. Murine left atrium lacks inotropic beta(2)-adrenoceptor function. We investigated whether beta(2)-adrenoceptors are involved in the cardiostimulant effects of (-)-adrenaline on spontaneously beating right atria and paced right ventricular myocardium of C57BL6 mice. We also studied a negative inotropic effect of (-)-adrenaline. 2. Sinoatrial tachycardia, evoked by (-)-adrenaline was resistant to blockade by beta(2)-selective ICI 118,551 (50 nM) but antagonized by beta(1)-selective CGP 20712A (300 nM). This pattern was unaffected by pretreatment with pertussis toxin (PTX, 600 microg kg(-1) i.p. 24 h) which reversed carbachol-evoked bradycardia to tachycardia. 3. Increases of ventricular force by (-)-adrenaline and (-)-noradrenaline were not blocked by ICI 118,551 but antagonized by CGP 20712A. 4. Under blockade of beta-adrenoceptors, (-)-adrenaline and (-)-noradrenaline depressed ventricular force (-logIC(50)M=7.7 and 6.9). The cardiodepressant effects of (-)-adrenaline were antagonized by phentolamine (1 microM) and prazosin (1 microM) but not by (-)-bupranolol (1 microM). Prazosin potentiated the positive inotropic effects of (-)-adrenaline (in the absence of beta-blockers) from -logEC(50)M=6.2 - 6.8. 5. PTX-treatment reduced carbachol-evoked depression of ventricular force in the presence of high catecholamine concentrations. Inhibition of ventricular function of G(i) protein was verified by 82% reduction of in vitro ADP-ribosylation. PTX-treatment tended to increase the positive inotropic potency of (-)-adrenaline under all conditions investigated, including the presence of ICI 118,551. 6. (-)-Adrenaline causes murine cardiostimulation through beta(1)-adrenoceptors but not through beta(2)-adrenoceptors. The negative inotropic effects of (-)-adrenaline are mediated through ventricular alpha(1)-adrenoceptors but not through beta(3)-adrenoceptors. Both G(i) protein and alpha(1)-adrenoceptors restrain (-)-adrenaline-evoked increases in right ventricular force mediated through beta(1)-adrenoceptors.
journal_name
Br J Pharmacoljournal_title
British journal of pharmacologyauthors
Heubach JF,Rau T,Eschenhagen T,Ravens U,Kaumann AJdoi
10.1038/sj.bjp.0704700subject
Has Abstractpub_date
2002-05-01 00:00:00pages
217-29issue
2eissn
0007-1188issn
1476-5381journal_volume
136pub_type
杂志文章abstract::1. Endothelial cells, under given circumstances, can initiate contraction (constriction) of the vascular smooth muscle cells that surround them. Such endothelium-dependent, acute increases in contractile tone can be due to the withdrawal of the production of nitric oxide, to the production of vasoconstrictor peptides ...
journal_title:British journal of pharmacology
pub_type: 杂志文章,评审
doi:10.1038/sj.bjp.0706042
更新日期:2005-02-01 00:00:00
abstract::Two prostaglandin endoperoxide analogues, EP 035 and EP 157, behave as specific thromboxane receptor antagonists on isolated smooth muscle preparations such as rabbit aorta, dog saphenous vein and guinea-pig trachea. However, in human platelet-rich plasma (PRP) they produce an unsurmountable block of aggregation induc...
journal_title:British journal of pharmacology
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doi:10.1111/j.1476-5381.1986.tb10196.x
更新日期:1986-03-01 00:00:00
abstract::1. The central and regional cardiovascular responses to intravenous (0.3, 1.0, 3.0 and 10.0 micrograms kg-1 min-1) and intracoronary (0.3, 0.9, 3.0 and 4.5 micrograms kg-1 min-1) infusions of elgodipine, a phenyldihydropyridine, and its solvent were studied in anaesthetized pigs. 2. Elgodipine (i.v.) caused dose-depen...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1990.tb14708.x
更新日期:1990-02-01 00:00:00
abstract::1. Morphine, oxotremorine and physostigmine showed antinociceptive activity in mice using the hot plate reaction time test.2. The action of morphine, but not that of oxotremorine, was antagonized by naloxone and by nalorphine, whereas the effect of physostigmine was unaffected by naloxone and increased by nalorphine.3...
journal_title:British journal of pharmacology
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doi:10.1111/j.1476-5381.1971.tb07205.x
更新日期:1971-12-01 00:00:00
abstract::1. The mechanisms of action of semotiadil fumarate, a novel Ca2+ antagonist, were examined by measuring the cytosolic Ca2+ level ([Ca2+]i) and force of contraction in porcine coronary arteries, and by determining [3H]-pyrilamine binding to bovine cerebellar membranes. 2. Semotiadil or verapamil (0.1 and 1 microM) inhi...
journal_title:British journal of pharmacology
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更新日期:1995-03-01 00:00:00
abstract::An attractive alternative to the use of direct agonists at the cannabinoid receptor type 1 (CB1) in the control of neuropathic pain may be to potentiate the actions of endogenous cannabinoids. Thus, the effects of AM404, an inhibitor of anandamide uptake, were assessed in an experimental model of neuropathic pain in r...
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pub_type: 杂志文章
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更新日期:2006-08-01 00:00:00
abstract::Preclinical and clinical data suggest that a modification in GABA(B) receptor expression and function may contribute to the symptoms of major depression and the response to antidepressants. This includes laboratory animal experiments demonstrating that antidepressants modify brain GABA(B) receptor expression and funct...
journal_title:British journal of pharmacology
pub_type: 杂志文章,评审
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更新日期:2011-01-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:1986-01-01 00:00:00
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更新日期:2012-06-01 00:00:00
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journal_title:British journal of pharmacology
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更新日期:2014-11-01 00:00:00
abstract::(1) UNC-49B and UNC-49C are gamma-aminobutyric acid (GABA) receptor subunits encoded by the Caenorhabditis elegans unc-49 gene. UNC-49B forms a homomeric GABA receptor, or can co-assemble with UNC-49C to form a heteromeric receptor. The pharmacological properties of UNC-49B homomers and UNC-49B/C heteromers were inves...
journal_title:British journal of pharmacology
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doi:10.1038/sj.bjp.0705119
更新日期:2003-03-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1980.tb07937.x
更新日期:1980-10-01 00:00:00
abstract::1. In circular smooth muscle of the guinea-pig gastric fundus, transmural nerve stimulation evoked a cholinergic excitatory junction potential (e.j.p.), and blockade of the e.j.p. by atropine revealed a non-adrenergic non-cholinergic (NANC) inhibitory junction potential (i.j.p.). 2. The amplitude of the e.j.p. was inc...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1996.tb15290.x
更新日期:1996-03-01 00:00:00
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doi:10.1038/sj.bjp.0704023
更新日期:2001-04-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1972.tb08146.x
更新日期:1972-11-01 00:00:00
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pub_type: 评论,杂志文章
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更新日期:2011-12-01 00:00:00
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更新日期:1999-01-01 00:00:00
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更新日期:2001-01-01 00:00:00
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doi:10.1111/j.1476-5381.1995.tb15030.x
更新日期:1995-08-01 00:00:00
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journal_title:British journal of pharmacology
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更新日期:2004-06-01 00:00:00
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journal_title:British journal of pharmacology
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doi:10.1111/j.1476-5381.1992.tb14392.x
更新日期:1992-07-01 00:00:00
abstract::The effects of aconitine, an Aconitum alkaloid, on spontaneous inhibitory and excitatory postsynaptic currents (IPSCs and EPSCs respectively) were investigated in the mechanically dissociated rat ventromedial hypothalamic (VMH) neurons in which native presynaptic nerve terminals remained intact. Under current-clamp co...
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更新日期:2002-02-01 00:00:00
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journal_title:British journal of pharmacology
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更新日期:1998-06-01 00:00:00
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journal_title:British journal of pharmacology
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doi:10.1038/sj.bjp.0707394
更新日期:2007-10-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:2003-09-01 00:00:00
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journal_title:British journal of pharmacology
pub_type: 杂志文章
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更新日期:2009-01-01 00:00:00
abstract::1. The effect of NMDA-receptor stimulation on phosphoinositide signalling in response to the metabotropic glutamate receptor agonist 1-aminocyclopentane-1S,3R-dicarboxylic acid (1S,3R-ACPD) has been examined in neonatal rat cerebral cortex slices. 2. Total [3H]-inositol phosphate ([3H]-InsPx) accumulation, in the pres...
journal_title:British journal of pharmacology
pub_type: 杂志文章
doi:10.1111/j.1476-5381.1994.tb13057.x
更新日期:1994-05-01 00:00:00
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pub_type: 杂志文章
doi:10.1038/sj.bjp.0701602
更新日期:1998-01-01 00:00:00