Abstract:
BACKGROUND:Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is expressed by immune cells and has been shown to play an important role in tumor surveillance due to its ability to induce apoptosis in various transformed cells. Interferon gamma (IFN-gamma), a multipotent cytokine with broad stimulatory effects on anti-tumoral immune reactions, may exert its cytotoxic activity directly on tumor cells or indirectly via stimulation of effector cells. This study was designed to determine the effect of IFN-gamma on TRAIL-mediated apoptosis in human colon carcinoma cell lines. MATERIALS AND METHODS:Cytotoxicity was assessed by MTT-assay. Expression of death receptors was measured by reverse transcriptase polymerase chain reaction. Apoptosis was assessed by caspase-8 immunoblot, DNA fragmentation and morphological studies. RESULTS:Treatment with TRAIL resulted in detectable cytotoxicity within 5 hours and was enhanced in a dose-dependent manner. When cells were pretreated with IFN-gamma, the cytotoxic effect of TRAIL increased significantly. Treated cells showed a typical apoptotic morphology that was accompanied by internucleosomal cleavage of DNA. Up-regulation of caspase-8 expression and activation were detected as a result of pretreatment with IFN-gamma and subsequent apoptosis mediated by TRAIL. CONCLUSION:Our results demonstrated that IFN-gamma sensitises human colon carcinoma cells to TRAIL-mediated apoptosis, partly by elevated caspase-8 expression.
journal_name
Anticancer Resjournal_title
Anticancer researchauthors
Langaas V,Shahzidi S,Johnsen JI,Smedsrød B,Sveinbjørnsson Bsubject
Has Abstractpub_date
2001-11-01 00:00:00pages
3733-8issue
6Aeissn
0250-7005issn
1791-7530journal_volume
21pub_type
杂志文章abstract::The neurogenic rat tumour BT4A was serially transplanted to the dorsum of the right hind leg of BD IX rats. The tumours were treated with water bath heating at 44.0 degrees C for one hour alone or combined with bleomycin or BCNU, both given intraperitoneally at a dose of 20 mg/kg. Bleomycin alone had no effect on tumo...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1982-11-01 00:00:00
abstract::Bisphosphonates are strong inhibitors of osteoclastic bone resorption in both benign and malignant bone diseases. The nitrogen-containing bisphosphonates (N-BPs) have strong cytotoxicity via inhibition of protein prenylation in the mevalonate pathway, and also demonstrate direct cytostatic and proapoptotic effects on ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-04-01 00:00:00
abstract::An adenovirus type 2-transformed rat embryo brain cell line (F 17 A2AS) and two of its subclones (F 17 CI, F 17 C2) have been studied using chromosome banding and flow cytometric techniques. In determining the modal chromosome number, a bimodal distribution was observed in each of the cell lines. In each case the firs...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1984-11-01 00:00:00
abstract::Screening for hereditary tumours provides a basis for secondary prevention. In 1985, the Foundation for the Detection of Hereditary Tumours was established in The Netherlands to coordinate screening in families with hereditary tumours. In the initial period of four years a large number of families were collected. Regi...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1990-03-01 00:00:00
abstract:BACKGROUND:Resistance to paclitaxel (PTX) is a major concern in treating ovarian cancer. Thus, agents effective in taxane-resistant tumours are highly sought. It has recently been shown that albendazole (ABZ) is a potent inhibitor of cell proliferation, angiogenesis and tumour growth. This study was designed to examine...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2009-10-01 00:00:00
abstract::Para-aminobenzoic acid (PABA) has been previously reported as being an inhibitor of DDP toxicity, and its use did not result in any observable loss in antitumor activity of DDP. The following studies investigated the effect of PABA on the pharmacokinetics and urinary excretion of cis-diamminedichloroplatinum(II) (DDP)...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1995-11-01 00:00:00
abstract:BACKGROUND/AIM:Changes in epidermal growth factor receptor (EGFR) are commonly found in cancer progression, signaling a poor outcome in patients. In the present study, we aimed to investigate whether nimotuzumab could be of benefit for cholangiocarcinoma (CCA) treatment. MATERIALS AND METHODS:The expression of EGFR wa...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11729
更新日期:2017-07-01 00:00:00
abstract:BACKGROUND:Active hexose-correlated compound (AHCC) is an extract of a basidiomycete mushroom that enhances the therapeutic effects and reduces the side-effects of chemotherapy. Our previous studies demonstrated that heat-shock protein 27 (HSP27) was involved in gemcitabine-resistance of pancreatic cancer cells and it ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2015-11-01 00:00:00
abstract::There is sparse information about cancer in the axillary tail of Spence (CATS). Eight hundred and thirty-nine patients with breast cancer were retrospectively studied for the occurrence of CATS. Ten patients were identified based on detection by imaging studies. A tendency towards stage II or III disease, and estrogen...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2012-09-01 00:00:00
abstract:BACKGROUND/AIM:Cancer incidence and mortalities are growing worldwide, therefore research and development of more effective and less invasive treatments, such as photodynamic therapy, are needed. Herein, we investigated the methylene blue (MB) photoactivation effects in lung epithelial cells (BEAS-2B) and lung adenocar...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.13522
更新日期:2019-07-01 00:00:00
abstract:BACKGROUND:C-reactive protein (CRP) is the prototypical biomarker of inflammation. Genetic variations within the CRP gene have been shown to be associated with alterations of CRP expression and prognosis in cancer patients. The purpose of this study was to evaluate the association between four polymorphisms of the CRP ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-06-01 00:00:00
abstract:BACKGROUND/AIM:FAM92A1-289 is recognized as one of the newly-discovered putative oncogenes. This study was performed to reveal its oncogenic functions in human cervical carcinoma cells. MATERIALS AND METHODS:The FAM92A1-289+ cell line was established with knock-in technique and selected by puromycin-resistance screeni...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.11090
更新日期:2016-10-01 00:00:00
abstract::The cell proliferation activity of sixteen childhood primitive neuroectodermal tumors (PNETs) was observed immunocytochemically, to determine the cell kinetics and cell proliferation activity of these relatively undifferentiated, malignant brain tumors. Two mouse anti-human monoclonal antibodies (MoABs) were employed ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Small cell lung cancer (SCLC) cells express cholinergic nicotinic receptors on their membranes, and the stimulatory effect on cell growth of nicotine has been described in cell cultures. We investigated three SCLC cell lines, all showing high levels of fluorescence intensity when labeled with FITC alpha-bungarotoxin i...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1996-11-01 00:00:00
abstract:BACKGROUND:Neolymphangiogenesis, a process of lymphatic vessel development in neoplastic tissue, may be a key event in the transmission of cancer cells into lymph nodes. The current study examined the relationship between lymphatic vessel density (LVD) measured by podoplanin (D2-40) expression, clinicopathological para...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2014-06-01 00:00:00
abstract:BACKGROUND/AIM:Despite numerous studies, the etiology of chronic lymphocytic leukemia (CLL) remains unknown. A hypothesis of autoantigen stimulation in leukemic clone selection might explain 'stereotypy' of B-cell receptors. In healthy cells, cofilin-1 (CFL1) has multiple functions. Its role was described in several ma...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14653
更新日期:2020-11-01 00:00:00
abstract::The aims of this study were to evaluate the pharmacokinetics, tolerability and hematopoietic toxicity of mitoxantrone in elderly women. Thirteen patients with advanced breast cancer, median age of 73 years, received escalating doses of mitoxantrone 8, 10, 12 and 14 mg/m2 on day 1, q 21. There was a linear relationship...
journal_title:Anticancer research
pub_type: 临床试验,杂志文章
doi:
更新日期:1999-01-01 00:00:00
abstract::Recurrent breast cancer (BC) tends to show an aggressive behavior and the number of cases of long-term survival is limited. Two rare cases of recurrent breast cancer were treated with a multidisciplinary approach. The outcome of the two cases indicated an excellent survival period of more than 13 years after recurrenc...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract:BACKGROUND:In order to decrease surgery-related morbidity, we evaluated the reliability of the evaluation of lymph node metastasis in patients with uterine corpus cancer by positron-emission tomography (PET) with 2-[(18)F]fluoro-2-deoxy-D-glucose (FDG) before surgical staging. MATERIALS AND METHODS:Patients with newly...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-09-01 00:00:00
abstract::Pro- and anti-apoptotic factors and intracellular signaling pathways are targets for therapeutic development of anticancer agents. Carboxyamido-triazole (CAI) is an inhibitor of transmembrane calcium influx and intracellular calcium-requiring signal transduction pathways. The present study investigates the effects of ...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2004-09-01 00:00:00
abstract:BACKGROUND:Solar ultraviolet-B (UVB) and vitamin D have been found inversely correlated with cancer incidence, mortality, and survival rates in many studies. MATERIALS AND METHODS:In this work, two sets of cancer data were used: age-adjusted cancer incidence and mortality rates in 2002 for 21 Western developed countri...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2006-07-01 00:00:00
abstract::Mechanisms of tumor growth are concerned with the possibility that neoplasms are disturbed in their response to growth factors. Interleukin 2 (IL 2) is secreted by activated T-cells and is required for autocrine T-cell growth. Controversy exists as to the functional capacity of T-lymphocytes in patients with B-cell ch...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1990-07-01 00:00:00
abstract:AIM:To evaluate the influence on relapse-free survival (RFS) and overall survival (OS) of a transitory protective stoma (TPS) at the time of complete cytoreductive surgery for advanced-stage ovarian cancer. PATIENTS AND METHODS:From our Institution, we retrospectively selected patients from 2006 to 2011 with ovarian c...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.12379
更新日期:2018-03-01 00:00:00
abstract::The interaction between sodium 5,6-benzylidene-L-ascorbate (SBA) and dopamine was investigated by three different parameters: radical intensity, prooxidant action and cytotoxic activity. Under alkaline conditions, SBA and dopamine produced doublet and quartet ESR signals, respectively. The addition of increasing conce...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1998-09-01 00:00:00
abstract:AIM:To assess whether prognostic factors in male (MBC) and female (FBC) breast cancer have similar impact on survival. PATIENTS AND METHODS:Charts for men and women diagnosed with breast cancer referred to the London Regional Cancer Program (LRCP) were reviewed. Patients with distant metastatic diseases were excluded....
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2013-05-01 00:00:00
abstract::Game theory can be a powerful tool for generating testable hypotheses concerning biological systems. We present a simple game that has many features analogous to a developing cellular system. The game mimics random turning on of genes in cells. Despite the randomness explicit in the mechanism, the game nonetheless res...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-11-01 00:00:00
abstract:BACKGROUND/AIM:Gefitinib and erlotinib were shown to be particularly effective in a clinically selected subpopulation of non-small cell lung cancer patients (NSCLC): adenocarcinoma histology, non-smoking status, Asian origin and female gender have been associated with improved clinical benefit compared to the unselecte...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND/AIM:MicroRNAs (miRNAs) regulate the development of colorectal cancer (CRC). We aimed to investigate miRNAs and their relation to cancer-related signaling pathways in site-specific CRC. MATERIALS AND METHODS:We used a total of 24 left- and right-sided Finnish CRC samples (discovery cohort) and The Cancer Gen...
journal_title:Anticancer research
pub_type: 杂志文章
doi:10.21873/anticanres.14360
更新日期:2020-07-01 00:00:00
abstract:BACKGROUND:A recent study suggested that the p53Arg (at residue 72) homozygous genotype could be a potential genetic risk factor for cervical cancer among white women. To confirm this result we examined the proportion of p53 genotypes in a larger number of patients with cervical cancer and in patients with squamous int...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1999-05-01 00:00:00
abstract::Drug resistance eventually limits the effectiveness of antiestrogens in breast cancer treatment. Pharmacological reversal of this refractoriness has been attempted with R-Verapamil, a well tolerated calcium channel blocker. This drug significantly decreased the incidence of lung foci after intravenous seeding of the R...
journal_title:Anticancer research
pub_type: 杂志文章
doi:
更新日期:1991-03-01 00:00:00