Abstract：
:The objective was to further test the hypothesis that aluminum (Al) citrate transport across the blood-brain barrier is mediated by a monocarboxylate transporter (MCT). Speciation calculations showed that Al citrates were the predominant Al species under the conditions employed. Al citrate did not inhibit lactate uptake and was not taken up by the rat erythrocyte, suggesting it does not serve as an effective substrate for either MCT1 or the anion exchanger. Studies were conducted with b.End5 cells derived from mouse brain endothelial cells to identify the properties of the carrier(s) mediating Al citrate transport. Western blot analysis of b.End5 cells showed expression of the transferrin receptor and MCT1, but not MCT2 or MCT4. Uptake of Al citrate was approximately 70% faster than citrate. Citrate and Al citrate uptake were sodium independent. Citrate uptake increased at pH 6.9 compared to 7.4, whereas Al citrate uptake did not. Al citrate uptake was reduced by inhibitors of mitochondrial respiration and oxidative phosphorylation, suggesting ATP dependence, but not by ouabain, suggesting no role for Na/K-ATPase. Uptake was not affected by alpha-ketoglutarate or malonate, substrates for the dicarboxylate carrier. Many substrates and inhibitors of MCT1 and organic anion transporters reduced Al citrate uptake into b.End5 cells. BSP and fluorescein, organic anion transporter substrates/inhibitors, inhibited Al citrate uptake. We conclude that Al citrate transport across the blood-brain barrier is carrier-mediated, involving either an uncharacterized MCT isoform expressed in the brain such as MCT7 or MCT8 and/or one of the many members of the organic anion transporting protein family, some of which are known to be expressed at the blood-brain barrier.
journal_name
Brain Resjournal_title
Brain researchauthors
Yokel RA,Wilson M,Harris WR,Halestrap APdoi
10.1016/s0006-8993(02)02234-5subject
Has Abstractpub_date
2002-03-15 00:00:00pages
101-10issue
1-2eissn
0006-8993issn
1872-6240pii
S0006899302022345journal_volume
930pub_type
杂志文章相关文献
BRAIN RESEARCH文献大全abstract：:Methylazoxymethanol acetate (MAM)-induced cortical hypoplasia resulted in a 2-fold increase in the concentration of norepinephrine and its metabolite, 3-methoxyl-4-hydroxyphenylglycol sulfate, and a 26% decrease in the Bmax for the beta-receptor ligand, [3H]dihydroalprenolol (DHA) in the rat cortex. Chronic treatment ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(82)90195-x
更新日期：1982-08-01 00:00:00
abstract：:Morphologically distinct subsets of mesotelencephalic neurons were sought following retrograde transport of Fluoro-Gold from iontophoretic injections relatively restricted to the medial shell, core or rostral pole subterritories of the nucleus accumbens. The diameter and lengths of Fluoro-Gold immunolabeled dendrites ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00556-6
更新日期：1995-08-14 00:00:00
abstract：:In acute cats deeply anesthetized with Nembutal, monosynaptic excitatory postsynaptic potentials (EPSPs) triggered by stimulation of the ventrolateral (VL) thalamic nucleus and the pes pedunculus were recorded in large pyramidal tract cells (PT cells). Deep anesthesia, low intensities of stimulation and an averaging t...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(79)90598-5
更新日期：1979-01-05 00:00:00
abstract：:The recent indications of specialized lateralization of cardiovascular regulation within the right and left posterior insular cortex of the rat, suggest the possibility of transcallosal connectivity between these regions. This has not been previously demonstrated using physiological techniques. Extracellular neural re...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(00)02068-0
更新日期：2000-04-28 00:00:00
abstract：:The effects of partial metabolic inhibition (induced by 2 h exposure to low concentrations of cyanide (NaCN)) on the glutamate receptor agonist alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA)-induced excitotoxicity and elevation of free cytoplasmic Ca2+ levels ([Ca2+]i) were studied in glucose-deprived pri...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)01123-2
更新日期：1998-01-01 00:00:00
abstract：:The characteristics of synaptic transmission in whole embryonic avian sympathetic ganglia have been examined by intracellular recording. Neurons in lumbar paravertebral ganglia of chick embryos exhibit both fast nicotinic excitatory postsynaptic potentials (EPSPs) and non-cholinergic slow EPSPs. Fast nicotinic transmi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(85)90073-2
更新日期：1985-09-01 00:00:00
abstract：:When the axons of goldfish retinal ganglion cells are severed the cell bodies undergo a series of changes as the axons regenerate. These changes begin to reverse when the axons start to innervate the tectum and by 3 months after the lesion the cell bodies have nearly returned to normal. When the axons projecting to th...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)90824-9
更新日期：1987-10-13 00:00:00
abstract：:Nitric oxide synthase (NOS) activity was examined in forebrain, cerebellum and optic lobes of adult domestic fowl, having a hereditary primary generalized convulsive disorder. NOS was approximately 2-fold higher in only the forebrain of adult epileptic fowl compared to non-epileptic (carrier) hatchmates. A significant...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(96)00595-1
更新日期：1996-10-07 00:00:00
abstract：:The present study provides evidence for the presence of an endogenous ligand for the phencyclidine (PCP) receptor of mammalian brain. Partially purified bovine hippocampal extracts potently and dose dependently inhibit binding to PCP receptors of [3H]N-(1-[2-thienyl]-cyclohexyl)piperidine (TCP), a highly potent and sp...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(87)91499-5
更新日期：1987-07-21 00:00:00
abstract：:Metabotropic glutamate receptors (mGluRs) are thought to mediate diverse processes in brain including synaptic plasticity and excitotoxicity. These receptors are often divided into three groups by their pharmacological profiles. [3H]Glutamate binding in the presence of compounds selective for ionotropic glutamate rece...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/s0006-8993(97)00818-4
更新日期：1997-10-31 00:00:00
abstract：:Early adverse events have been shown to increase the incidence of interstitial cystitis/painful bladder syndrome in adulthood. Despite high clinical relevance and reports of stress-related symptom exacerbation, animal models investigating the contribution of early life stress to female urological pain are lacking. We ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2016.02.039
更新日期：2016-05-15 00:00:00
abstract：:The binding of the adenosine receptor agonists, [3H]N-ethylcarboxamidoadenosine (NECA) and [3H]cyclohexyladenosine (CHA) to membrane preparations and to cryostat sections of the rat brain was examined. The xanthine derivative, 1,3-dipropyl-8-cyclopentylxanthine (DPCPX) was ca. 500-fold more effective at A1 than at A2 ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90589-5
更新日期：1986-03-19 00:00:00
abstract：:Transport of the hexapeptide dalargin across the blood-brain barrier was accomplished using a nanoparticle formulation. The formulation consisted of dalargin bound to poly(butyl cyanoacrylate) nanoparticles by sorption, coated with polysorbate 80. Intravenous injection of this formulation to mice resulted in an analge...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00023-j
更新日期：1995-03-13 00:00:00
abstract：:Previous studies have revealed that there are increases in the incorporation of [3H]amino acids into protein in the denervated neuropil of the dentate gyrus during periods of reactive synaptogenesis. The present study evaluates whether the increase in incorporation reflects an increase in protein synthetic machinery (...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0169-328x(87)90032-5
更新日期：1987-09-01 00:00:00
abstract：:The connectivity of the stellate ganglion of Octopus was investigated using cobalt and horseradish peroxidase (HRP) tracers applied to severed preganglionic and postganglionic nerves. Centripetal cells, defined as cells in the ganglion which send their axons back toward the brain, were discovered by both methods. Appl...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(80)90583-1
更新日期：1980-02-17 00:00:00
abstract：:Previous studies have found that the primary change in the brain of chronic alcoholics is a selective loss of white matter in cerebral hemispheres. It is unknown whether the loss of white matter is due to alcohol-induced degeneration of axons in white matter, and this hypothesis has never been tested. We used a newly ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2004.09.035
更新日期：2004-12-17 00:00:00
abstract：:Muscle spindles are induced during embryonic development by contact between sensory axons and undifferentiated myotubes. Neonatal guinea pigs deprived of nerve growth factor in utero can have only 15-20% of the normal number of dorsal root ganglion cells. This study demonstrates that such neonates have normal numbers ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)90945-6
更新日期：1988-03-22 00:00:00
abstract：:Microtubule organization in type I hair cells has been investigated during the synaptogenesis of vestibular receptors in mammals. The different steps in the maturation of the synapse between the hair cell and the nerve chalice were: a slight symmetrical membrane densification; the appearance of synaptic bodies alongsi...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(86)90161-6
更新日期：1986-02-01 00:00:00
abstract：:Male Long-Evans rats were administered nicotine bitartrate or sodium tartrate either during adolescence (p29-43) or adulthood (p80-94). Route of administration was via subcutaneously implanted osmotic pump (initial dose 2.0 mg/kg/day, free base). Five weeks following nicotine administration, brains were processed for ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.03.019
更新日期：2007-06-02 00:00:00
abstract：:Previous studies from our laboratory suggest that apolipoprotein (apoE), a lipid transporting protein, facilitates olfactory nerve regeneration. We have shown that apoE is enriched in the olfactory nerve and around the glomeruli of the olfactory bulb (OB). The studies reported herein were undertaken to identify possib...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2006.12.036
更新日期：2007-03-16 00:00:00
abstract：:The concentration of soluble glial fibrillary acidic (GFA) protein and the specific activity of glutamine synthetase (GS) were estimated in 11 central nervous system (CNS) regions of the 90-day-old rat. Marked differences were observed in the regional distribution of these astrocyte marker proteins. The striatum and s...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(85)90708-5
更新日期：1985-04-01 00:00:00
abstract：:The quantitative autoradiographic method with L-(35S)methionine was used to determine the effects of 1-acute (4h) and 2-chronic (14 days) immobilization stress followed by one week of recovery. Acute stress induced a significant decrease in methionine incorporation into proteins in 17 of the 35 brain structures examin...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(95)00046-s
更新日期：1995-03-27 00:00:00
abstract：:The effect of dopamine (DA) on the calcium-activated potassium conductance underlying the slow afterhyperpolarization (AHP) which follows a train of action potentials in hippocampal pyramidal cells was studied utilizing the in vitro hippocampal slice preparation. Bath-applied DA (1-100 microM) significantly reduced th...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(86)90773-0
更新日期：1986-08-06 00:00:00
abstract：:In urethane-anesthetized rats, high frequency stimulation was applied to the medial perforant pathway at various time intervals (50, 100, 200, 350 and 500 ms) following stimulation of the same pathway by a single pulse of equal intensity. Recordings of dentate gyrus granule cell evoked responses were made to investiga...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(88)91358-3
更新日期：1988-01-12 00:00:00
abstract：:The blood-nerve barrier consists of continuous layers of cells linked by tight junctions and includes the endothelial cells which line the endoneurial capillaries and the perineurial cells which surround fascicles of nerve fibers. A facilitated transport carrier protein allows D-glucose to penetrate the barrier. To de...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(90)91115-w
更新日期：1990-01-29 00:00:00
abstract：:Parkin Co-Regulated Gene (PACRG) is a novel gene that is oriented in a head-to-head array with parkin, and expression of the two genes is regulated by a shared bi-directional promoter. Mutations in parkin are the most common cause of early-onset autosomal recessive Parkinson's disease, however the function of PACRG an...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2008.01.050
更新日期：2008-03-27 00:00:00
abstract：:Histone proteins from purified nuclei of neonatal and adult mouse retinas were analyzed and compared utilizing SDS-polyacrylamide gel electrophoresis. Identical procedures were applied to examine the histones extracted from the brains of the same animals. In the newborn and mature retina and brain, 8 histone fractions...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0165-3806(87)90150-7
更新日期：1987-06-01 00:00:00
abstract：:YM-14673 is a thyrotropin-releasing hormone (TRH) analog that has a modification of the N-terminal (pyroglutamyl) moiety of the tripeptide. It is longer acting than TRH and has substantially greater potency with regard to central facilitatory activity. In the present studies, the effect of YM-14673 was examined in rat...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/0006-8993(89)90509-x
更新日期：1989-05-08 00:00:00
abstract：:Previous studies have suggested that substance P (SP) plays a critical role in the development of brain oedema and functional deficits following traumatic brain injury and that SP receptor antagonism may improve outcome. No studies have described such a role in ischemic stroke. The present study characterized the effe...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2011.03.066
更新日期：2011-06-01 00:00:00
abstract：:Glutamate is the main excitatory neurotransmitter in the central nervous system. This amino acid mediates learning and memory processes acting through ionotropic and metabotropic receptor binding. Metabotropic glutamate receptors (mGluRs) are G protein-coupled receptors that stimulate phospholipase C (PLC) or inhibit ...
journal_title：Brain research
pub_type： 杂志文章
doi：10.1016/j.brainres.2007.01.010
更新日期：2007-06-11 00:00:00
