A three-residue signal confers localization of a reporter protein in the inner nuclear membrane.

abstract：:The post-translational modification of histones has been implicated in the regulation of cellular lifespan. Previously, we reported that cellular aging is associated with increased ubiquitylation of histone H2B and methylation of histone H3 at lysines 4 and 79 in yeast telomeric heterochromatin. Here, we show the anta...

journal_title：Biochemical and biophysical research communications

authors： Ryu HY,Rhie BH,Ahn SH

更新日期：2014-03-28 00:00:00

abstract：:Ritonavir, an inhibitor of HIV-1 protease, has been reported to also inhibit the Ca2+-dependent cysteine protease, calpain. We have investigated these claims with an in vitro study of the effect of ritonavir on the m-calpain and mu-calpain isoforms. Ritonavir failed to block either autolytic or hydrolytic calpain acti...

journal_title：Biochemical and biophysical research communications

authors： Cuerrier D,Nie Z,Badley AD,Davies PL

更新日期：2005-02-04 00:00:00

abstract：:Interactions between C1q and other subunits of C1 were analyzed by sucrose gradient ultracentrifugation. A zone of dilute, radioiodine labelled C1q was sedimented through uniform concentrations of either C1r2C1s2, C1r2, C1r2 or C1s(2). The dissociation constants were found to be 3 x 10(-9) M and 6 x 10(-9) M for C1r2C...

journal_title：Biochemical and biophysical research communications

authors： Lakatos S

更新日期：1987-12-16 00:00:00

abstract：:Aldosterone and osmotic stress are well known to regulate the epithelial Na(+) channel (ENaC)-mediated Na(+) transport in renal epithelial cells. However, we have no information on how aldosterone and osmotic stress interact on stimulation of ENaC-mediated Na(+) transport in renal epithelium. In the present report, we...

journal_title：Biochemical and biophysical research communications

authors： Niisato N,Taruno A,Marunaka Y

更新日期：2007-09-14 00:00:00

abstract：:We designed an experimental approach to differentiate the kinetics of protein binding to a lipid membrane from the kinetics of the associated conformational change in the protein. We measured the fluorescence intensity of the single Trp6 in chicken liver bile acid-binding protein (L-BABP) as a function of time after m...

journal_title：Biochemical and biophysical research communications

authors： Galassi V,Nolan V,Villarreal MA,Perduca M,Monaco HL,Montich GG

更新日期：2009-05-15 00:00:00

abstract：:To identify the source of arachidonic acid utilized for eicosanoid production, rabbit alveolar macrophages were challenged with 1.0 microM 12-O-tetradecanoylphorbol-13-acetate (TPA) or 3.8 microM Ca+2 ionophore A23187 for 3 h. Upon stimulation with TPA, a loss of [3H]arachidonic acid from phosphatidylcholine, phosphat...

journal_title：Biochemical and biophysical research communications

authors： Cochran FR,Connor JR,Roddick VL,Waite BM

更新日期：1985-07-31 00:00:00

abstract：:The death of nigral neurons in Parkinson's disease is thought to involve the formation of the endogenous neurotoxin, 5-S-cysteinyl-dopamine. In the present study, we show that the polyphenols, (+)-catechin and caffeic acid, which contain a catechol moiety, inhibit tyrosinase-induced formation of 5-S-cysteinyl-dopamine...

journal_title：Biochemical and biophysical research communications

authors： Vauzour D,Vafeiadou K,Spencer JP

更新日期：2007-10-19 00:00:00

abstract：:The nuclear-mitochondrial stringent response was examined in isonuclear rho+, 21S rRNA-containing rho-, and rho o strains of S. cerevisiae. By 30 min after nutritional downshift, nuclear rDNA transcription falls to 15% of control levels congruently in all strains, as assayed via whole-cell RNA or by hybrid selection o...

journal_title：Biochemical and biophysical research communications

authors： McEntee CM,Cantwell R,Thomas LC,Hudson AP

更新日期：1989-10-16 00:00:00

abstract：:Zinc-finger antiviral protein (ZAP) is a recently isolated host antiviral factor that inhibits the replication of many viruses such as Moloney murine leukemia virus (MLV) and Sindbis virus (SIN) by preventing the accumulation of viral mRNA in the cytoplasm. ZAP comprises four CCCH zinc-finger motifs, the second and fo...

journal_title：Biochemical and biophysical research communications

authors： Jeong MS,Kim EJ,Jang SB

更新日期：2010-06-04 00:00:00

abstract：:Protein markers induced by hormones are the necessary probes to study hormone regulation of gene expression. We have previously shown that ACTH was able to induce one of these markers in the cytosol of the rat adrenal: protein E (Dazord et al, Biochem. J., 176, 233-239, 1978). In this paper by using adrenal mRNA from ...

journal_title：Biochemical and biophysical research communications

authors： Langlois-Gallet D,Dazord A

更新日期：1984-03-30 00:00:00

abstract：:The role of small ras homologous GTP-binding proteins in the regulation of smooth muscle contractility has become increasingly apparent but there is still little information about the presence of these proteins in human uterine smooth muscle. Messenger RNAs for p21-activated protein kinase isoforms (PAK1, PAK2, and PA...

journal_title：Biochemical and biophysical research communications

authors： Moore F,Da Silva C,Wilde JI,Smarason A,Watson SP,López Bernal A

更新日期：2000-03-16 00:00:00

abstract：:The phenothiazine antipsychotic drug chlorpromazine, a potent calmodulin antagonist, inhibited human natural killer cell-mediated cytotoxicity in a dose-dependent manner. This inhibition of natural killer cell activity was not due to interference with conjugate formation, nor was it due to toxicity to the natural kill...

journal_title：Biochemical and biophysical research communications

authors： Steele TA,Brahmi Z

更新日期：1988-09-15 00:00:00

abstract：:The cobalt(II) derivative of cucumber basic blue copper protein "plantacyanin" has been prepared. The visible absorption, circular dichroic and magnetic circular dichroic spectra of Co(II)-plantacyanin are similar to those of Co(II)-plastocyanin, indicating that the stereochemistry of Co(II) is tetrahedral and at leas...

journal_title：Biochemical and biophysical research communications

authors： Sakurai T

更新日期：1986-09-30 00:00:00

abstract：:Whereas sitosterol and 24(28)-methylene cycloartanol were competitive inhibitors (with Ki = 26 microM and 14 microM, respectively), 24(R,S)-25-epiminolanosterol was found to be a potent non-competitive inhibitor (Ki = 3.0 nM) of the S-adenosyl-L-methionine-C-24 methyl transferase from sunflower embryos. Because the gr...

journal_title：Biochemical and biophysical research communications

authors： Nes WD,Janssen GG,Norton RA,Kalinowska M,Crumley FG,Tal B,Bergenstrahle A,Jonsson L

更新日期：1991-05-31 00:00:00

abstract：:Dynamic shaping of the antibody repertoire is essential for effective immunity. We describe here a novel approach for clarifying how the antibody repertoire is shaped over time for development of a specific immune response. We obtained over 500 immunoglobulin G1 clones harboring VH186.2 from the spleen, bone marrow, a...

journal_title：Biochemical and biophysical research communications

authors： Furukawa A,Furukawa K,Azuma T

更新日期：2004-06-25 00:00:00

abstract：:Corticosterone methyloxidase I (CMO I) deficiency is an autosomal recessive disorder of aldosterone biosynthesis. To determine further the molecular genetic basis of CMO I deficiency, a patient of Turkish origin that suffered from CMO I deficiency was studied. Nucleotide sequencing of the PCR-amplified exons from the ...

journal_title：Biochemical and biophysical research communications

authors： Nomoto S,Massa G,Mitani F,Ishimura Y,Miyahara K,Toda K,Nagano I,Yamashiro T,Ogoshi S,Fukata J,Onishi S,Hashimoto K,Doi Y,Imura H,Shizuta Y

更新日期：1997-05-19 00:00:00

abstract：:Schistosomiasis represents one of the most prevalent parasitic infections affecting over 249 million people worldwide. The pathological damage is mainly caused by the eggs laid by female schistosomes. Zinc finger proteins (ZFPs) usually play critical roles in many biological functions. In this study, we cloned, identi...

journal_title：Biochemical and biophysical research communications

authors： Zhang R,Li J,Xiang M,Hong Q,Xu B,Hu W

更新日期：2018-07-02 00:00:00

abstract：:A secondary structure has been predicted for the hemorrhagic metalloproteases using a method developed in Zurich that extracts structural information from patterns of conservation and variation in homologous protein sequences. This prediction tests the limits of the method when applied to a small number of homologous ...

journal_title：Biochemical and biophysical research communications

authors： Gerloff DL,Jenny TF,Knecht LJ,Benner SA

更新日期：1993-07-15 00:00:00

abstract：:ARD1 protein is a mammalian gene product homologous to a yeast Ard1p (Arrest defective 1 protein) acetyltransferase. Although two alternative splicing products of ARD1, ARD1(235) and ARD1(225), were reported in mouse, only ARD1(235) orthologue was reported in humans. Here we show that ARD1(225) is not expressed in hum...

journal_title：Biochemical and biophysical research communications

authors： Chun KH,Cho SJ,Choi JS,Kim SH,Kim KW,Lee SK

更新日期：2007-02-02 00:00:00

abstract：:Human monomorphic and polymorphic arylamine acetyltransferases (EC 2.3.1.5) were expressed in monkey kidney COS-1 cells and used to study the N- and O-acetylation of a number of carcinogenic amines and their N-hydroxy metabolites. The monomorphic enzyme N-acetylated the aromatic amines, 2-aminofluorene and 4-aminobiph...

journal_title：Biochemical and biophysical research communications

authors： Minchin RF,Reeves PT,Teitel CH,McManus ME,Mojarrabi B,Ilett KF,Kadlubar FF

更新日期：1992-06-30 00:00:00

abstract：:Diallyl trisulfide (DATS), one of the volatile constituents of garlic oil, has been reported to possess antioxidant, anti-inflammatory, and anti-carcinogenic properties. In this study, DATS (10μmol) given orally for 7days before and for another 7days after starting administration of 2.5% dextran sulfate sodium (DSS) i...

journal_title：Biochemical and biophysical research communications

authors： Lee HJ,Lee HG,Choi KS,Surh YJ,Na HK

更新日期：2013-07-26 00:00:00

abstract：:The diagnostic significance of phosphoglycerate mutase (PGM) B-type isozyme activity capable of being inducible under hypoxia at the gene level as a serum marker for cerebral stroke was investigated. The normal level (mean +/- 2 SD) in human serum was determined to be 38 +/- 18 units/L. Within 2 h after the onset of c...

journal_title：Biochemical and biophysical research communications

authors： Hayashi T,Matuo Y

更新日期：2001-10-05 00:00:00

abstract：:During the late stage of homologous recombination in prokaryotes, RuvA binds to the Holliday junction intermediate and executes branch migration in association with RuvB. The RuvA subunits form two distinct complexes with the Holliday junction: complex I with the single RuvA tetramer on one side of the four way juncti...

journal_title：Biochemical and biophysical research communications

authors： Mayanagi K,Fujiwara Y,Miyata T,Morikawa K

更新日期：2008-01-11 00:00:00

abstract：:Hematopoiesis is a complex process tightly controlled by sets of transcription factors in a context-dependent and stage-specific manner. Smad2/3 transcription factor plays a central role in differentiation and survival of erythroid cells. Here we report that follistatin-like 1 (FSTL1) treatment impairs hemin-induced e...

journal_title：Biochemical and biophysical research communications

authors： Wu J,Dong Y,Teng X,Cheng M,Shen Z,Chen W

更新日期：2015-10-30 00:00:00

abstract：:The effect of endothelin-1 (ET-1) on secretion and synthesis of rat atrial natriuretic peptide (rANP) as well as its mRNA levels was studied in primary cultures of neonatal rat atrial cardiocytes. ET-1 dose-dependently (10(-10)-10(-7) M) increased media and cellular rANP-like immunoreactivity as well as its cytoplasmi...

journal_title：Biochemical and biophysical research communications

authors： Fukuda Y,Hirata Y,Taketani S,Kojima T,Oikawa S,Nakazato H,Kobayashi Y

更新日期：1989-11-15 00:00:00

abstract：:To clarify the role of hepatocyte growth factor (HGF) in embryonic lung development, organoids from fetal rat lung were cultured in collagen gels with or without HGF antisense oligonucleotides. Cyst-like structures formed within 24 h in organoids isolated from fetuses after 14 days' gestation, but this was abolished b...

journal_title：Biochemical and biophysical research communications

authors： Itakura A,Kurauchi O,Morikawa S,Okamura M,Furugori K,Mizutani S

更新日期：1997-12-08 00:00:00

abstract：:We have examined the steady-state levels of intermediate filament mRNA in amyotrophic lateral sclerosis using the RNAse protection assay (NFL, NFM, NFH; corrected against GAPDH) or by PCR (peripherin, alpha-internexin, nestin, and vimentin; corrected against beta-actin). Significant elevations of NFL and peripherin mR...

journal_title：Biochemical and biophysical research communications

authors： Strong MJ,Leystra-Lantz C,Ge WW

更新日期：2004-04-02 00:00:00

abstract：:A chemical inhibitor library of 84 compounds was screened to investigate the signaling pathway(s) leading to activation of Nrf2 in response to nitric oxide (NO). We identified the protein kinase C delta (PKCdelta) inhibitor rottlerin as the only compound that reduced NO-induced ARE-luciferase reporter activity and dim...

journal_title：Biochemical and biophysical research communications

authors： Zhang J,Hung AC,Ng PY,Nakayama K,Hu Y,Li B,Porter AG,Dhakshinamoorthy S

更新日期：2009-09-04 00:00:00

abstract：:The complete covalent structure of the gamma 2 subunit of bovine brain G proteins was determined by peptide mapping using matrix-assisted laser desorption ionization and electrospray ionization mass spectrometry and by partial sequencing using tandem mass spectrometry. Fragments were identified in proteolytic digests ...

journal_title：Biochemical and biophysical research communications

authors： Wilcox MD,Schey KL,Busman M,Hildebrandt JD

更新日期：1995-07-17 00:00:00

abstract：:Skinned muscle fibers prepared from fetal rabbit heart (28 days of gestation) showed a marked resistance to acidic pH in the Ca(2+) regulation of force generation, compared to the fibers prepared from adult heart. SDS-PAGE and immunoblot analysis showed that the slow skeletal troponin I was predominantly expressed in ...

journal_title：Biochemical and biophysical research communications

authors： Morimoto S,Goto T

更新日期：2000-01-27 00:00:00