Abstract：
:The Thermotoga maritima aldolase gene has been cloned into a T7 expression vector and overexpressed in Escherichia coli. The preparation yields 470 UL(-1) of enzyme at a specific activity of 9.4 U mg(-1). During retroaldol cleavage of KDPG, the enzyme shows a k(cat) that decreases with decreasing temperature. A more than offsetting decrease in K(m) yields an enzyme that is more efficient at 40 degrees C than at 70 degrees C. The substrate specificity of the enzyme was evaluated in the synthetic direction with a range of aldehyde substrates. Although the protein shows considerable structural homology to KDPG aldolases from mesophilic sources, significant differences in substrate specificity exist. A preparative scale reaction between 2-pyridine carboxaldehyde and pyruvate provided product of the same absolute configuration as mesophilic enzymes, but with diminished stereoselectivity.
journal_name
Bioorg Med Chemjournal_title
Bioorganic & medicinal chemistryauthors
Griffiths JS,Wymer NJ,Njolito E,Niranjanakumari S,Fierke CA,Toone EJdoi
10.1016/s0968-0896(01)00307-8subject
Has Abstractpub_date
2002-03-01 00:00:00pages
545-50issue
3eissn
0968-0896issn
1464-3391pii
S0968089601003078journal_volume
10pub_type
杂志文章abstract：:Six new polyketides aplojaveediins A-F (1-6) were isolated from the endophytic fungus Aplosporella javeedii associated with the host plant Orychophragmus violaceus (Brassicaceae). The structures of the new metabolites were elucidated by analysis of their NMR and MS data. Compound 1 exhibited antifungal activity agains...
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更新日期：2009-01-01 00:00:00
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更新日期：2020-04-01 00:00:00
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更新日期：2013-10-15 00:00:00
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journal_title：Bioorganic & medicinal chemistry
pub_type： 杂志文章
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更新日期：2013-05-01 00:00:00
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journal_title：Bioorganic & medicinal chemistry
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