Abstract:
:Experimental Doxorubicin-exposure in utero is correlated with foetal oesophageal atresia, tracheo-oesophageal fistula, axial alterations. While gastrointestinal and respiratory defects have been largely investigated, only sporadic data have been published to date on notochordal and vertebral defects. The aim of this work was the study of the genesis of chordal and vertebral abnormalities in rat embryos and foetuses exposed to Doxorubicin and the study of their correlation with oesophageal and tracheal defects. For this purpose, pregnant rats were i.p. injected with saline (control) or with 4 mg/Kg b.w. Doxorubicin on days 9.5 and 10.5 of gestation. Embryos and foetuses were morphologically analysed on days 10.5-15 and 16, 18, 20 of gestation respectively, fixed in formaldehyde and histologically processed. Slides were routinely stained with haematoxylin-eosin (11-15 days post coitum embryos and all foetuses) or specifically stained with aniline blue for the staining of basal laminae (10.5 days post coitum embryos). Moreover, some foetuses at term (20 days post coitum) were processed for bone and cartilage staining. The data obtained in the present work confirm the specificity of Doxorubicin in inducing gastro-intestinal and tracheal defects, describe the genesis of these defects step by step, describe the type and the genesis of notochordal abnormalities and their fate and exclude the role of Doxorubicin in inducing axial skeletal malformations.
journal_name
Histol Histopatholjournal_title
Histology and histopathologyauthors
Menegola E,Broccia ML,Di RFdoi
10.14670/HH-17.31subject
Has Abstractpub_date
2002-01-01 00:00:00pages
31-8issue
1eissn
0213-3911issn
1699-5848journal_volume
17pub_type
杂志文章abstract::Representative tumour sections from 468 patients with invasive breast cancer were immunostained for cyclooxygenase-2 (COX-2) and evaluated. The relationships between COX-2 expression, clinical outcome and various clinicopathological variables, including tumour vascularity and disseminated tumour cells (DTC) in the bon...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-27.1315
更新日期:2012-10-01 00:00:00
abstract:BACKGROUND:Honey and olive oil are natural products that have high nutritional values, and therapeutic properties. Cytotoxic drugs, like methotrexate (MTX) are used to treat malignancies in tumour cells; however, these drugs also have serious side effects that could threaten the patient's life. AIM:To evaluate the pot...
journal_title:Histology and histopathology
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doi:10.14670/HH-18-126
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abstract::The nerve fiber distribution in the epidermis of the hairless rat skin was studied light microscopically by means of zinc iodide-osmium tetroxide staining. Two different morphological types of free nerve fiber endings could be detected: clusters of relatively thick nerve fibers stretched up through the spinous layer u...
journal_title:Histology and histopathology
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doi:10.14670/HH-15.493
更新日期:2000-04-01 00:00:00
abstract::Macrophages are important effector cells in cell-mediated immunity against intracellular infection. Among cytokines that macrophages are able to release are IL-12 and TNF alpha. IL-12 is a critical linker between the innate and adaptive cell-mediated immunity, capable of Th1 differentiation and IFN gamma release by T ...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-15.199
更新日期:2000-01-01 00:00:00
abstract::A proportion of anaplastic large cell tumours is difficult to classify on sections of routinely processed, paraffin-embedded tissue. Differentiation into large cell lymphoma, carcinoma, melanoma or sarcoma is important in order to assess prognosis and proper treatment. Although the use of immunohistochemistry has been...
journal_title:Histology and histopathology
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abstract::This paper reports the histopathological findings in the eyes of a 26-year-old female patient diagnosed with systemic lupus erythematosus (SLE) with peripheral neuropathy. The patient had no significant ocular problems. She died of pneumonia after two years of suffering. The eyeballs were procured at autopsy and the r...
journal_title:Histology and histopathology
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doi:10.14670/HH-20.373
更新日期:2005-04-01 00:00:00
abstract::The vacuolar-type H(+)-ATPases (V-ATPases) are a family of multi-subunit ATP-dependent proton pumps involved in diverse cellular processes, including acid/base homeostasis, receptor-mediated endocytosis, processing of proteins and signaling molecules, targeting of lysosomal enzymes, and activation of various degradati...
journal_title:Histology and histopathology
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doi:10.14670/HH-25.1611
更新日期:2010-12-01 00:00:00
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journal_title:Histology and histopathology
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doi:10.14670/HH-27.529
更新日期:2012-04-01 00:00:00
abstract::Cancer is the subject of intense research around the world, but many questions about how the disease works remain unanswered. How exactly does cancer start and how do tumours grow? In fact, at present there are ten times more anticancer drugs being tested in clinical trials than there were 15 years ago. However, many ...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-27.157
更新日期:2012-02-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-22.399
更新日期:2007-04-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-18.111
更新日期:2003-01-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1996-07-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:
更新日期:1997-10-01 00:00:00
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journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1995-10-01 00:00:00
abstract::Versican, a large extracellular matrix proteoglycan accumulates in tumor stroma and plays a key role in both malignant transformation and tumor progression. Increased versican expression has been observed in a wide range of malignant tumors, and has been associated with both cancer relapse and poor patient outcomes in...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-28.701
更新日期:2013-06-01 00:00:00
abstract::Mitochondrial transcription factor A (mtTFA) plays a crucial role in both the transcription and maintenance of mitochondrial DNA. A high expression of mtTFA has been demonstrated in several solid tumors, and is closely associated with cancer cell survival/apoptosis and growth. However, its expression pattern in pancre...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-30.193
更新日期:2015-02-01 00:00:00
abstract::Hexachlorobenzene (HCB) is a fungicide and a pollutant of increasing concern in industrialized regions of the world. Reproductive failure is one of the effects of HCB upon mammals. Alteration of cell shape in the ovary surface epithelium (OSE) of Cynomolgus monkeys following oral administration of HCB was observed in ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1991-10-01 00:00:00
abstract::The effects of cadmium exposure on the protein secretory functions of cultured neonatal rat hepatocytes were analyzed by both two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and electron microscopy. [35S]Methionine-labelled protein secretion was significantly depressed by cadmium exposure in a dose-depend...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1991-01-01 00:00:00
abstract::Voltage-activated Ca2+ channels play an important role in synaptic transmission, signal processing and development. The immunohistochemical localization of Cav1.2 (alpha1C) and Cav2.3 (alpha1E) Ca2+ channels was studied in the developing and adult mouse organ of Corti using subunit-specific antibodies and fluorescent ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-18.1115
更新日期:2003-10-01 00:00:00
abstract::The terms construct or tissue equivalent refer to neotissue produced by tissue engineering techniques. The elements forming the construct are scaffolds on which cells are "recreated" to form an engineered-tissue sensitive to certain cell signals. The ability of the cells to expand and differentiate on the scaffold is ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-20.891
更新日期:2005-07-01 00:00:00
abstract::Earlier we reported that atherosclerotic lesions of apoE-deficient mice contained cells which stained positively with anti-S-100 antibody and that cells exhibiting the ultrastructural features of dendritic cells were present in the aortic lesions. These observations suggested that dendritic cells might be involved in ...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-16.801
更新日期:2001-07-01 00:00:00
abstract::F10, a subline of the B16 mouse melanoma cell line, is itself the parent of the more metastatic BL6 line. BL6 cells differ from F10 cells by an alteration of the gene encoding the B56gamma regulatory subunit of protein phosphatase 2A (PP2A), which results in mRNA encoding a truncated variant of the subunit (deltagamma...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-19.391
更新日期:2004-04-01 00:00:00
abstract:BACKGROUND:Expression of hypoxia-related tissue factors in 1p-aberrant oligodendroglial neoplasms diminishes patient outcome. Differentiated embryo-chondrocyte expressed gene 1 (DEC1) has been described as novel hypoxia-related tissue factor. In our study, we assessed the expression of DEC1 in 1p aberrant oligodendrogl...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-20.1173
更新日期:2005-10-01 00:00:00
abstract::The resistance to airflow that develops in most obstructive processes takes place in the small airways. The aim of the present paper is to describe bronchial hysteresis morphometrically in a respiratory cycle model. As a working hypothesis, it is proposed that the changes that take place in the respiratory tract durin...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-18.19
更新日期:2003-01-01 00:00:00
abstract::The immunohistochemical distribution of 15 blood coagulation factors in the germinal centers (GCs) of human Peyer's patches (PPs) was studied. Although factor VIII, active alpha-thrombin, and fibrinogen were hardly evident in the GCs, the majority of coagulation factors, such as kallikrein, high-molecular-weight kinin...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1992-04-01 00:00:00
abstract::Our study aimed to investigate the association of Pyruvate Kinase isozyme type M2 (PKM2) with radiation resistance in locally advanced cervical squamous cell carcinoma (LACSCC). We retrospectively reviewed 132 female patients who received primary radiation therapy to treat LACSCC at Federation Internationale of Gyneco...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-11-627
更新日期:2015-11-01 00:00:00
abstract::Griffonia simplicifolia agglutinin I-B4 (GSAI-B4) has broader specificity for B antigen variants and can recognize the antigens in a wide variety of human tissues. Thus, the concentration range of GSAI-B4 required for staining and the susceptibility of staining to alpha-galactosidase digestion is presumed to correlate...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:
更新日期:1997-04-01 00:00:00
abstract::A novel family of evolutionally conserved neuronal guidance cues, including ligands (i.e., Slit, netrin, epherin, and semaphorin) and their corresponding receptors (i.e., Robo, DCC/Unc5, Eph and plexin/ neuropilin), has been identified to play a crucial role in axon pathfinding and branching as well as neuronal cell m...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-25.1181
更新日期:2010-09-01 00:00:00
abstract::Telocytes (TCs) represent a new distinct type of interstitial cells found in many organs, including lungs. TCs are mainly defined by a small cellular body from which arise very long (hundreds of micrometers) extensions named telopodes. During the last years, TCs were characterized in respect with their microRNA profil...
journal_title:Histology and histopathology
pub_type: 杂志文章,评审
doi:10.14670/HH-11-807
更新日期:2016-12-01 00:00:00
abstract::Vascular endothelial cells play a critical role in the maintenance of endometrial homeostasis. Indeed many pathological conditions causing abnormal endometrial bleeding including progestin only contraception, hormone replacement therapy, endometrial polyps, myomas, hyperplasia and cancer are associated with aberrant a...
journal_title:Histology and histopathology
pub_type: 杂志文章
doi:10.14670/HH-20.719
更新日期:2005-07-01 00:00:00