Abstract:
:Detection of Haemophilus ducreyi in genital ulcer specimens by culture lacks sensitivity. To enhance detection, a heminested polymerase chain reaction (PCR) assay was developed targeting the nucleotide sequence of a gene, designated p27, which encodes for a 27 kDa H. ducreyi-specific protein. The p27 PCR assay detected all (37/37) H. ducreyi strains tested and gave no amplified product from DNA extracts of any of 31 other microorganisms, from 30 non-genital ulcer specimens, or from 29 urethral and vaginal swab specimens collected from non-chancroid STD patients. In genital ulcer disease specimens, compared to combined positive results obtained by culture and a previously described PCR assay, the p27 PCR assay showed a sensitivity of 91% (48/53). The p27 PCR assay provides a specific and a sensitive detection of H. ducreyi in clinical specimens.
journal_name
Int J STD AIDSjournal_title
International journal of STD & AIDSauthors
Tekle-Michael T,Van Dyck E,Abdellati S,Laga Mdoi
10.1258/0956462011924425subject
Has Abstractpub_date
2001-12-01 00:00:00pages
797-803issue
12eissn
0956-4624issn
1758-1052journal_volume
12pub_type
杂志文章abstract:SUMMARY:Point-of-care microscopy is the gold standard for the diagnosis of vaginal discharge in genitourinary (GU) medicine clinics but not used in primary care settings and reproductive health clinics to which many patients present. In our GU medicine clinic setting, we conducted an audit to assess the utility of micr...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/ijsa.2008.008212
更新日期:2008-12-01 00:00:00
abstract::The objective of the study was to determine the association of neurocognitive impairment with health-related quality of life (HRQoL) in patients receiving highly active antiretroviral therapy (HAART). Seventy subjects were cross-sectionally analysed with a standardized neuropsychological test battery and a questionnai...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/095646204773557794
更新日期:2004-04-01 00:00:00
abstract::Domestic violence (DV) occurs frequently within the UK, with one in four women and one in six men experiencing DV during their life. DV is the leading cause of morbidity for women aged 19-44 years and is associated with sexual health problems. However, few data exist on the prevalence of DV in UK genitourinary (GU) me...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1177/0956462412472799
更新日期:2013-05-01 00:00:00
abstract::Self-inserted vaginal tampons for the molecular diagnosis of non-ulcerative STIs were evaluated. Cervical and vaginal swabs, tampons and urines were collected from 185 first-time antenatal clinic attendees. Cultures and nucleic acid amplification assays (NAA) were performed. The sensitivity of PCR on tampons for Trich...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/095646204322764271
更新日期:2004-02-01 00:00:00
abstract::This study investigated the epidemiological and clinical characteristics of hepatitis B virus (HBV) in HIV-infected adults at the time of antiretroviral therapy (ART) initiation in Guangdong province, China. A total of 2793 HIV-infected adults were enrolled between January 2004 and September 2011. Demographic data and...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1177/0956462415600570
更新日期:2016-09-01 00:00:00
abstract::We assessed the validity of self-reported sex and condom use by comparing self-reports with prostate-specific antigen (PSA) detection in a prospective study of 210 female sex workers in Mombasa, Kenya. Participants were interviewed on recent sexual behaviours at baseline and 12-month follow-up visits. At both visits, ...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/095646207779949899
更新日期:2007-01-01 00:00:00
abstract::Neisseria gonorrhoeae (GC) and Chlamydia trachomatis (CT) are two of the most prevalent bacterial sexually transmitted infections in the UK. Although the high burden of extra-genital infections with GC and CT in men who have sex with men has been well established, a significant number of extra-genital site infections ...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1177/0956462414543120
更新日期:2015-06-01 00:00:00
abstract::Patients were recruited for hypnotherapy from a clinic for patients with frequently recurrent genital herpes simplex virus (rgHSV). Psychological and immunological parameters were measured 6 weeks prior to hypnotherapy and 6 weeks afterwards, during which time each patient kept a diary of symptoms of rgHSV. Following ...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/0956462991913420
更新日期:1999-11-01 00:00:00
abstract::This study assessed lubricant use during receptive anal intercourse (RAI) among Peruvian men who have sex with men (MSM) and willingness to use a hypothetical rectal microbicide (RM) formulated as a lubricant to prevent HIV infection. Data were collected from 843 Peruvian MSM for the 2008 HIV Sentinel Surveillance usi...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/ijsa.2010.010134
更新日期:2010-08-01 00:00:00
abstract::In Ireland the incidence of sexually transmitted infections (STIs) is steadily increasing while the number of new HIV-diagnoses in men who have sex with men has more than doubled in the past decade. This study investigated the prevalence of STIs in asymptomatic HIV-infected men who have sex with men (MSM) attending a ...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1177/0956462414521165
更新日期:2014-09-01 00:00:00
abstract::Home treatment with podophyllotoxin or imiquimod are commonly prescribed therapies for anogenital warts. It is important to ascertain if patients are locating all lesions for treatment and if they know when they are clear of them. We set out to assess patients' ability to determine the number and location of their gen...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1177/095646240401500508
更新日期:2004-05-01 00:00:00
abstract::Cervical cancer is the leading incident cancer and the main cause of cancer-related mortality among women in sub-Saharan Africa. Furthermore, HIV-infected women are at a higher risk of developing cervical cancer than HIV-negative women. The purpose of this study was to distinguish differences in characteristics of HIV...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1177/0956462415606252
更新日期:2016-10-01 00:00:00
abstract::Toxoplasma encephalitis (TE) is usually diagnosed in advanced stages of HIV infection when the CD4+ count is <100-200 cells/µl. A 55-year-old woman with HIV/AIDS, well controlled on antiretroviral therapy (ART), CD4+ count in the 300 cells/μl range for >1 year presented with acute onset of headache, nausea and vomitin...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1177/0956462413506891
更新日期:2014-04-01 00:00:00
abstract::The aim of this study was to investigate the factors relating to CD4 level at HIV diagnosis and HIV testing behaviour. Participants were newly diagnosed patients (n = 654) in Japan from 2000 to 2005. Around 75% of participants were diagnosed at hospital and clinics. Mean CD4 counts at diagnosis through voluntary HIV t...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/ijsa.2009.009493
更新日期:2012-04-01 00:00:00
abstract::Between June 1996-September 1997 and December 1998-December 1999, two surveys using an anonymous questionnaire were carried out in Europe among persons living with HIV infection. The questionnaire included questions on use of antiretrovirals, complementary or alternative medicines. Vitamins/minerals were taken by 528 ...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/095646203322387929
更新日期:2003-10-01 00:00:00
abstract::Squamous cell carcinoma of the penis is an uncommon cancer, though in one study it accounted for 90% of all penile cancers. Its association with balanitis xerotica obliterans (BXO) is a rare though recognized occurrence. We describe a case of a 46-year-old Caucasian male who first presented to our open-access clinic w...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/095646206775455658
更新日期:2006-02-01 00:00:00
abstract::Little is known about the incidence of bacterial sexually transmitted infections (STIs) among HIV-infected versus HIV-uninfected adolescents. This secondary analysis of a national, multisite study included adolescents aged 12-18 years who were behaviourally HIV-infected (n = 346) or HIV-uninfected but at-risk (n = 182...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1177/0956462412472425
更新日期:2013-02-01 00:00:00
abstract::There are few data on the impact of antiretroviral therapy (ART) regimen factors on adherence in ART-naïve HIV patients on contemporary once- or twice-daily regimens. Ninety-nine newly diagnosed patients in a prospective observational cohort study completed a visual analogue scale to assess their ART adherence. Adhere...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/ijsa.2011.011292
更新日期:2012-05-01 00:00:00
abstract::In order to evaluate the occurrence of hepatotoxicity in patients treated with antiretroviral therapy (ART) who switch protease inhibitor (PI), and the role of viral hepatitis in its development, we performed a retrospective study on 182 HIV patients treated with ART for 24 months. The presence of hepatitis viruses an...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/0956462053057585
更新日期:2005-02-01 00:00:00
abstract::High annual figures of sexually transmitted infections (STIs) are diagnosed in the Netherlands despite significant efforts to control them. Herein, we analyse trends and determinants of STI diagnoses, co-infections, and sexual risks among visitors of 26 STI clinics between 2007 and 2011. We recorded increased positivi...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1177/0956462413491736
更新日期:2014-01-01 00:00:00
abstract::The objectives were, first, to determine the sensitivity and specificity of the Osmetech Microbial Analyser (OMA) for the diagnosis of bacterial vaginosis (BV) and, secondly, to determine the factors that interfere with the performance of the test. Women presenting to a genitourinary medicine clinic underwent routine ...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/095646203321156881
更新日期:2003-02-01 00:00:00
abstract::The objective of the study was to compare the impact of oral and i.v. ganciclovir on resource use and direct health care costs, from the perspective of the UK National Health Service (NHS). The analytical framework used was cost analysis. The sources of data were an open, randomized clinical trial, and additional rese...
journal_title:International journal of STD & AIDS
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
doi:10.1258/0956462961918383
更新日期:1996-10-01 00:00:00
abstract::We describe a recent outbreak of syphilis in young heterosexuals in the north west of England. A cluster of 12 cases of syphilis (7 primary and 5 early latent) was identified in Rochdale in heterosexuals aged 20 or under. Nine were women. Five were asymptomatic at presentation. This outbreak occurred in a group not us...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/ijsa.2011.011031
更新日期:2011-09-01 00:00:00
abstract::In Jamaica, where homosexuality is criminalized, scant research has examined associations between sexual stigma and HIV infection. The study objective was to examine correlates of HIV infection among men who have sex with men (MSM) in Jamaica. We conducted a cross-sectional tablet-based survey with MSM in Jamaica usin...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1177/0956462417717652
更新日期:2018-01-01 00:00:00
abstract::Sexually transmitted infections (STIs) in Vietnam have been increasing. Control of STIs among female sex workers (FSWs) is important in controlling the epidemic. Effective STI control requires that physicians are skilful in taking sexual history for FSW patients. Three hundred and seventy-one physicians responded to a...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1177/0956462414529553
更新日期:2015-01-01 00:00:00
abstract::The aim of this work was to study the human papillomavirus (HPV) type-distribution in condylomata acuminata (CA) of mainland China and to estimate the potential role of HPV prophylactic vaccines for CA in mainland China. Forty-three studies using polymerase chain reaction to detect HPV were included in this meta-analy...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章,meta分析
doi:10.1258/ijsa.2008.008040
更新日期:2008-10-01 00:00:00
abstract::Pubic hair grooming is common in many countries, but little is known about this practice in Africa. Grooming has been positively associated with self-reported sexually transmitted infections (STIs). This study aimed to investigate the prevalence and safety of pubic hair grooming in two South African settings. In KwaZu...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1177/0956462420941709
更新日期:2020-10-01 00:00:00
abstract::This paper describes the effect of HIV prevention intervention among female sex workers in Delhi. Over a 2-year period we found a marked increase in condom use with little increase in HIV seroprevalence. The prevalence of HIV infection did not increase during the study period (1 positive among 701 in 1988 vs 1 positiv...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1177/095646249400500113
更新日期:1994-01-01 00:00:00
abstract::Cytomegalovirus (CMV) colitis is a well recognized complication of advanced HIV disease and is only rarely diagnosed in patients with normal immune function. A case of CMV colitis occurring in early HIV infection is described. Although CMV infection is normally confined to patients with advanced HIV disease, it is pos...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/0956462001915183
更新日期:2000-11-01 00:00:00
abstract::Blood lipids and high-sensitivity C-reactive protein (hsCRP) are used to assess cardiovascular disease (CVD) risk. We evaluated in a cross-sectional design the relationship of hsCRP to markers of liver function (aspartate and alanine transaminases [AST and ALT, respectively]), CVD risk factors and HIV-disease progress...
journal_title:International journal of STD & AIDS
pub_type: 杂志文章
doi:10.1258/ijsa.2007.007207
更新日期:2008-06-01 00:00:00