Development of a heminested polymerase chain reaction assay for the detection of Haemophilus ducreyi in clinical specimens.

Abstract:

:Detection of Haemophilus ducreyi in genital ulcer specimens by culture lacks sensitivity. To enhance detection, a heminested polymerase chain reaction (PCR) assay was developed targeting the nucleotide sequence of a gene, designated p27, which encodes for a 27 kDa H. ducreyi-specific protein. The p27 PCR assay detected all (37/37) H. ducreyi strains tested and gave no amplified product from DNA extracts of any of 31 other microorganisms, from 30 non-genital ulcer specimens, or from 29 urethral and vaginal swab specimens collected from non-chancroid STD patients. In genital ulcer disease specimens, compared to combined positive results obtained by culture and a previously described PCR assay, the p27 PCR assay showed a sensitivity of 91% (48/53). The p27 PCR assay provides a specific and a sensitive detection of H. ducreyi in clinical specimens.

journal_name

Int J STD AIDS

authors

Tekle-Michael T,Van Dyck E,Abdellati S,Laga M

doi

10.1258/0956462011924425

subject

Has Abstract

pub_date

2001-12-01 00:00:00

pages

797-803

issue

12

eissn

0956-4624

issn

1758-1052

journal_volume

12

pub_type

杂志文章
  • The uptake of HIV and syphilis testing in a nurse-delivered service during Gay Pride events.

    abstract::Novel HIV testing strategies may improve the uptake of the test. The aim of the present study was to investigate the uptake of HIV testing during Birmingham Pride events in 2009 and 2010. The project was a collaboration between Whittall Street Clinic and Healthy Gay Life. This was a nurse-delivered service that was of...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/ijsa.2012.012023

    authors: Manavi K,Williams G,Newton R

    更新日期:2012-12-01 00:00:00

  • Impact of antiretroviral dosing frequency and pill burden on adherence among newly diagnosed, antiretroviral-naive HIV patients.

    abstract::There are few data on the impact of antiretroviral therapy (ART) regimen factors on adherence in ART-naïve HIV patients on contemporary once- or twice-daily regimens. Ninety-nine newly diagnosed patients in a prospective observational cohort study completed a visual analogue scale to assess their ART adherence. Adhere...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/ijsa.2011.011292

    authors: Buscher A,Hartman C,Kallen MA,Giordano TP

    更新日期:2012-05-01 00:00:00

  • Emerging sexually transmitted viral infections: 1. Review of Ebola virus disease.

    abstract::This is the first in a series of articles reviewing four viral infections, Ebola virus, Zika virus, human T-cell lymphotropic virus, type 1 and hepatitis C virus, with an emphasis on recent advances in our understanding of their sexual transmission. With current day speed and ease of travel it is important for staff i...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章,评审

    doi:10.1177/0956462417703572

    authors: Caswell RJ,Manavi K

    更新日期:2017-11-01 00:00:00

  • Penile carcinoma arising in balanitis xerotica obliterans.

    abstract::Squamous cell carcinoma of the penis is an uncommon cancer, though in one study it accounted for 90% of all penile cancers. Its association with balanitis xerotica obliterans (BXO) is a rare though recognized occurrence. We describe a case of a 46-year-old Caucasian male who first presented to our open-access clinic w...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/095646206775455658

    authors: Goolamali SI,Pakianathan M

    更新日期:2006-02-01 00:00:00

  • Surveillance of an infectious syphilis outbreak in south London, UK: a comparison of reporting systems.

    abstract::Rising heterosexual syphilis in south London in 2002 prompted Local Enhanced Syphilis Surveillance (LESS) in five of the 10 genitourinary medicine clinics. LESS reported a fall in heterosexual infectious syphilis in 2004 that was corroborated by the National Enhanced Syphilis Surveillance (NESS). However, mandatory cl...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/ijsa.2008.008216

    authors: Menon-Johansson AS,Young Y,Maguire H,Trail P,Ward H

    更新日期:2009-03-01 00:00:00

  • Genital elephantiasis and sexually transmitted infections - revisited.

    abstract::Genital elephantiasis is an important medical problem in the tropics. It usually affects young and productive age group, and is associated with physical disability and extreme mental anguish. The majority of cases are due to filariasis; however, a small but significant proportion of patients develop genital elephantia...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章,评审

    doi:10.1258/095646206775809150

    authors: Gupta S,Ajith C,Kanwar AJ,Sehgal VN,Kumar B,Mete U

    更新日期:2006-03-01 00:00:00

  • The use of complementary and alternative medicine by persons with HIV infection in Europe.

    abstract::Between June 1996-September 1997 and December 1998-December 1999, two surveys using an anonymous questionnaire were carried out in Europe among persons living with HIV infection. The questionnaire included questions on use of antiretrovirals, complementary or alternative medicines. Vitamins/minerals were taken by 528 ...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/095646203322387929

    authors: Colebunders R,Dreezen C,Florence E,Pelgrom Y,Schrooten W,Eurosupport Study Group.

    更新日期:2003-10-01 00:00:00

  • Herpes zoster and HIV infection in Tanzania.

    abstract::Two hundred consecutive patients with herpes zoster attending the skin clinic at the Kilimanjaro Christian Medical Centre (KCMC) were examined and checked for HIV infection. They ranged in age from 10 months to 86 years with the majority in their 20s and 30s. The dermatomes involved were thoracic (97), trigeminal (50)...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/0956462001915804

    authors: Naburi AE,Leppard B

    更新日期:2000-04-01 00:00:00

  • Blood-taking practices and needle-stick injuries in house officers.

    abstract::The blood-taking practices and prevalence of needle-stick injuries were recorded for 46 house officers working at the two largest teaching hospitals in Sheffield and 38 house officers working at three hospitals in London. Wearing gloves when performing venesection or inserting an intravenous cannula was generally perc...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1177/095646249100200109

    authors: Woolley PD,Palfreeman AJ,Patel R,Talbot MD,Samarasinghe PL

    更新日期:1991-01-01 00:00:00

  • Particular clinical presentations of psoriasis in HIV patients.

    abstract::Patients with HIV infection have a higher propensity for infectious, inflammatory, neoplastic and metabolic disorders. The link with psoriasis vulgaris is explained by well-known pathophysiological phenomena and can be observed at any stage of infection. The aim of our study was to characterize the clinical spectrum o...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/ijsa.2011.010525

    authors: Fernandes S,Pinto GM,Cardoso J

    更新日期:2011-11-01 00:00:00

  • Genital elephantiasis.

    abstract::Genital elephantiasis (esthiomene), which is the dramatic end-result of lymphatic obstruction, is rather rare. Although mainly associated with filariasis and sexually transmitted diseases, such as lymphogranuloma venereum and donovanosis, it could also be an uncommon complication of tubercular lymphadenitis, a common ...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/095646202760029886

    authors: Sarkar R,Kaur C,Thami GP,Kanwar AJ

    更新日期:2002-06-01 00:00:00

  • Can data from HIV voluntary counselling and testing be used to assess the impact of public health interventions? A literature review.

    abstract::Routinely collected data from clinical sites offering voluntary counselling and testing (VCT) for HIV diagnosis have been used to evaluate the impact of public health interventions; however, there has been considerable diversity in strategies. To gain an understanding of the outcome of these evaluations and provide th...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章,评审

    doi:10.1258/ijsa.2009.009005

    authors: Guy RJ,Prybylski D,Fairley CK,Hellard ME,Kaldor JM

    更新日期:2009-06-01 00:00:00

  • Fatal Kaposi's sarcoma-associated immune reconstitution following HAART initiation.

    abstract::This unique report describes a fatal case of immune reconstitution syndrome associated with Kaposi's sarcoma (KS). This case also illustrates the possibility of cryptic visceral KS with minimal cutaneous disease, as well as the potential for differential responses of cutaneous and visceral KS to treatment. ...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/0956462052932647

    authors: Crane HM,Deubner H,Huang JC,Swanson PE,Harrington RD

    更新日期:2005-01-01 00:00:00

  • Sexual behaviour in the city of Lisbon.

    abstract::This study of sexual behaviour in the city of Lisbon is based on data obtained from 2 random samples, one of individuals aged 16-20 (n = 400) and another of individuals over the age of 20 (n = 400). Samples have been stratified by district, gender and age. Data were obtained by personal interview and by questionnaires...

    journal_title:International journal of STD & AIDS

    pub_type: 临床试验,杂志文章,随机对照试验

    doi:10.1177/095646249500600108

    authors: Amaro F,Dantas AM,Teles Lda C

    更新日期:1995-01-01 00:00:00

  • What are the needs of asylum seekers attending an STI clinic and are they significantly different from those of British patients?

    abstract::To elicit the needs of asylum seekers attending the Royal Hallamshire genitourinary medicine clinic for the first time in 2002 and whether these differed from British patients, a search of patient records for 2002 identified 43 asylum seekers (21 female, 22 male) who were paired with 43 British patients matched by age...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/0956462041558230

    authors: Rogstad KE,Dale H

    更新日期:2004-08-01 00:00:00

  • Lack of behavioural risk factors for squamous intraepithelial lesions (SIL) in HIV-infected women.

    abstract::HIV-infected women have a high prevalence of abnormal Papanicolaou smears and cervical intraepithelial neoplasia. A multiparametric analysis of epidemiological and behavioural risk factors has been performed in a cohort of 204 HIV-infected women in an outpatient clinic with the aim to investigate risk factors associat...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/0956462971920316

    authors: Heard I,Jeannel D,Bergeron C,Saada M,Henrion R,Kazatchkine MD

    更新日期:1997-06-01 00:00:00

  • Human papillomavirus type-distribution in condylomata acuminata of mainland China: a meta-analysis.

    abstract::The aim of this work was to study the human papillomavirus (HPV) type-distribution in condylomata acuminata (CA) of mainland China and to estimate the potential role of HPV prophylactic vaccines for CA in mainland China. Forty-three studies using polymerase chain reaction to detect HPV were included in this meta-analy...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章,meta分析

    doi:10.1258/ijsa.2008.008040

    authors: Wang H,Qiao YL

    更新日期:2008-10-01 00:00:00

  • Are there any differences between patients attending a genitourinary medicine clinic with appointments and those without?

    abstract::The aim of this study is to identify the demographic and diagnostic characteristics of patients attending a genitourinary medicine clinic with booked appointments and compare them with those of walk-in attendees. During a period of three months, 749 patients were seen with new episodes; 61% of them had booked appointm...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/ijsa.2008.008308

    authors: Elawad BB

    更新日期:2009-03-01 00:00:00

  • Fuckbuddy partnerships among men who have sex with men - a marker of sexually transmitted infection risk.

    abstract::'Fuckbuddies' are a type of regular sexual partner with whom men have ongoing sexual contact, generally in the absence of romantic attachment. We surveyed 989 men who have sex with men (MSM) at the Melbourne Sexual Health Centre, Australia, with the aim of determining the frequency of 'fuckbuddy' partnerships among se...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1177/0956462417717647

    authors: Cornelisse VJ,Fairley CK,Phillips T,Walker S,Chow EP

    更新日期:2018-01-01 00:00:00

  • Unrealistic optimism about becoming infected with HIV: different causes in different populations.

    abstract::People tend to believe that their chance of experiencing undesirable events is lower and their chance of experiencing desirable events is higher than that of the average person like them. Two explanatory models of such 'unrealistic optimism' (UO) have been proposed: While the motivational account holds that UO serves ...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/095646206775809169

    authors: Gold RS

    更新日期:2006-03-01 00:00:00

  • Romantic love and sexually transmitted infection acquisition: hypothesis and review.

    abstract::Rates of sexually transmitted infections (STI) in the UK continue to increase. A discrepancy between knowledge and awareness of STIs, and sexual behaviour appears to be ubiquitous throughout the world. We hypothesize that human beings are biologically programmed to fall in love and bond in powerful relationships, whic...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章,评审

    doi:10.1258/0956462054944435

    authors: Goldmeier D,Richardson D

    更新日期:2005-09-01 00:00:00

  • Detecting asymptomatic Trichomonas vaginalis in females using the BD ProbeTec™ Trichomonas vaginalis Qx nucleic acid amplification test.

    abstract::Trichomonas vaginalis (TV) rates in women are increasing and many are asymptomatic. Nucleic acid amplification tests (NAATs) are becoming the 'gold standard' for diagnosis. We aimed to establish our asymptomatic TV rates by testing all women attending Oxfordshire's Sexual Health service, regardless of symptoms, using ...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1177/0956462416649888

    authors: Lord E,Newnham T,Dorrell L,Jesuthasan G,Clarke L,Jeffery K,Sherrard J

    更新日期:2017-03-01 00:00:00

  • Unexpected high prevalence of herpes simplex virus (HSV) type 2 seropositivity and HSV genital shedding in pregnant women living in an East Paris suburban area.

    abstract::Both herpes simplex virus type 2 (HSV-2) seroprevalence and the proportion of HSV-1 genital ulcers are increasing in industrialized countries. The consequences of these epidemiological changes, in pregnant women in France, for both the genital shedding of HSV and vertical transmission, have been poorly evaluated. The ...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/095646207781568457

    authors: LeGoff J,Saussereau E,Boulanger MC,Chemin C,Si-Mohamed A,Bélec L,Maisonneuve L

    更新日期:2007-09-01 00:00:00

  • Use of the Internet for home testing for Chlamydia trachomatis in Sweden: who are the users?

    abstract::Sweden was the first country to introduce online Chlamydia trachomatis testing for both men and women and this article provides information about the user characteristics and which risk groups are reached with this method. During the years 2005-2007 a questionnaire was administered with a response rate of 86% (n = 602...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/ijsa.2011.011030

    authors: Novak D,Novak M

    更新日期:2012-02-01 00:00:00

  • Spectrum of opportunistic infections in hospitalized HIV-infected patients in Phnom Penh, Cambodia.

    abstract::The aim of the study was to provide more comprehensive data on the clinical characteristics of hospitalized AIDS patients in Cambodia. Chart review of 381 HIV-infected patients admitted to a public hospital in Phnom Penh, Cambodia between December 1999 and May 2000 was performed. The in-hospital mortality rate was 43....

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/095646203765371312

    authors: Senya C,Mehta A,Harwell JI,Pugatch D,Flanigan T,Mayer KH

    更新日期:2003-06-01 00:00:00

  • Depression and HIV risk behaviors among adolescent girls and young women seeking family planning services in Western Kenya.

    abstract::We assessed prevalence of human immunodeficiency virus (HIV) risk behaviors and depressive symptoms among adolescent girls and young women (AGYW) aged 15-24 years attending four public family planning clinics in Western Kenya from January to June 2019. Moderate-to-severe depression (MSD) was defined as a Center for Ep...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1177/0956462420920423

    authors: Larsen A,Kinuthia J,Lagat H,Sila J,Abuna F,Kohler P,John-Stewart G,Pintye J

    更新日期:2020-06-01 00:00:00

  • Characterizing viral subtypes to assess patterns of HIV transmission.

    abstract::We characterized HIV-1 subtypes among 204 persons newly diagnosed with HIV in Ontario from 2003 to 2005 using samples from the Canadian HIV Strain and Drug Resistance Surveillance Program. We examined HIV-1 subtype by demographic characteristics and exposure category, and determined independent predictors of infection...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/ijsa.2009.009303

    authors: Rank C,Njihia JG,Remis RS,Shah L,Swantee C,Brooks JI,Jayaraman GC,Archibald CP

    更新日期:2012-10-01 00:00:00

  • Secondary syphilis masquerading as lupus vulgaris in an HIV-infected patient: A diagnosis suggested by histology.

    abstract::We report a case of secondary syphilis mimicking lupus vulgaris in an HIV-infected patient. A 21-year-old Brazilian man presented with a two-month history of asymptomatic cutaneous lesions accompanied by fever and fatigue. Dermatological evaluation revealed an erythematous, crusted, large plaque on the neck with the '...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1177/0956462418795590

    authors: Genovese G,Nazzaro G,Coggi A,Gianotti R,Ramoni S,Cusini M

    更新日期:2018-12-01 00:00:00

  • Seasonal variation in mortality of Pneumocystis jirovecii pneumonia in HIV-infected patients.

    abstract::A seasonal variation in the presentation of Pneumocystis jirovecii pneumonia (PCP) has been reported and a previous study from this centre noted a seasonal variation in mortality rates. This study examined seasonal influences (including climatic factors) within-host factors (clinical and laboratory-derived variables),...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1258/ijsa.2010.010148

    authors: Miller RF,Evans HE,Copas AJ,Huggett JF,Edwards SG,Walzer PD

    更新日期:2010-07-01 00:00:00

  • Provision of HIV test results by telephone is both safe and efficient for men who have sex with men.

    abstract::The aim of this study was to assess the impact of delivering HIV test results by telephone on HIV testing and subsequent risk behaviour of men, as well as saving on clinic consultation time. It was conducted at the Melbourne Sexual Health Centre, the main public sexual health clinic servicing Victoria, Australia. In 2...

    journal_title:International journal of STD & AIDS

    pub_type: 杂志文章

    doi:10.1177/0956462415623912

    authors: Bissessor M,Bradshaw CS,Fairley CK,Chen MY,Chow EP

    更新日期:2017-01-01 00:00:00