Development of a heminested polymerase chain reaction assay for the detection of Haemophilus ducreyi in clinical specimens.

Abstract:

:Detection of Haemophilus ducreyi in genital ulcer specimens by culture lacks sensitivity. To enhance detection, a heminested polymerase chain reaction (PCR) assay was developed targeting the nucleotide sequence of a gene, designated p27, which encodes for a 27 kDa H. ducreyi-specific protein. The p27 PCR assay detected all (37/37) H. ducreyi strains tested and gave no amplified product from DNA extracts of any of 31 other microorganisms, from 30 non-genital ulcer specimens, or from 29 urethral and vaginal swab specimens collected from non-chancroid STD patients. In genital ulcer disease specimens, compared to combined positive results obtained by culture and a previously described PCR assay, the p27 PCR assay showed a sensitivity of 91% (48/53). The p27 PCR assay provides a specific and a sensitive detection of H. ducreyi in clinical specimens.

journal_name

Int J STD AIDS

authors

Tekle-Michael T,Van Dyck E,Abdellati S,Laga M

doi

10.1258/0956462011924425

subject

Has Abstract

pub_date

2001-12-01 00:00:00

pages

797-803

issue

12

eissn

0956-4624

issn

1758-1052

journal_volume

12

pub_type

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