Abstract:
:A new approach is reported that combines synchrotron radiolysis and mass spectrometry to probe the structure of proteins. Hydroxyl radicals produced upon the radiolysis of protein solutions using synchrotron light modify amino acid side-chains on millisecond timescales. This results in the formation of stable oxidation products where the level of oxidation at the reactive residues is influenced by the accessibility of their side-chains to the bulk solvent. The aromatic and sulphur-containing residues have been found to react preferentially in accord with previous peptide studies. The sites of oxidation have been determined by tandem mass spectrometry. The rate of oxidation at these reactive markers has been measured for a number of proteolytic peptides as a function of exposure time based on the relative proportion of modified and unmodified peptide ions detected by mass spectrometry. Oxidation rates correlate closely with a theoretical measure of the accessibility of residue side-chains to the solvent in the native protein structure. This approach can distinguish the relative accessibility of the tryptophan residue side-chains of lysozyme at positions 62 and 123 from each other and all other tryptophan residues, and phenylalanine at position 34 from phenylalanine residues at positions 3 and 38 based upon their rates of oxidation.
journal_name
Int J Radiat Bioljournal_title
International journal of radiation biologyauthors
Kiselar JG,Maleknia SD,Sullivan M,Downard KM,Chance MRdoi
10.1080/09553000110094805subject
Has Abstractpub_date
2002-02-01 00:00:00pages
101-14issue
2eissn
0955-3002issn
1362-3095journal_volume
78pub_type
杂志文章abstract::Experimental data for acute radiation-induced skin reactions are reviewed. These show that for dose fractionation schedules with gaps, repopulation is initiated after a lag period. After this lag period, the isoeffective dose for a given level of skin reaction first increases rapidly, but then slows. The timing of the...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553000310001600907
更新日期:2003-07-01 00:00:00
abstract::A comparison has been made of the in vitro light-activated drug cytotoxicities of two different porphyrin compounds, Photofrin II and TPPS4. An early passage human colon adenocarcinoma cell line, WiDr, has been exposed to either drug for 24 h, the excess drug washed from the cells and the cells irradiated with light u...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553008814552061
更新日期:1988-10-01 00:00:00
abstract::A review is presented to the program of education and training setup within the DoReMi Network of Excellence. DoReMi was funded by Euratom under the EU 7th Framework Programme to coordinate the EU research into risks from low-dose ionizing radiation. It was seen to be necessary to form a network of expert institutions...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2018.1454616
更新日期:2019-01-01 00:00:00
abstract:PURPOSE:Terrorist attacks, with their intent to maximize psychological and economic damage as well as inflicting sickness and death on given targeted populations, are an ever-growing worldwide concern in government and public sectors as they become more frequent, violent, and sensational. If given the chance, it is lik...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553002.2017.1332440
更新日期:2017-09-01 00:00:00
abstract:PURPOSE:The radiation-induced bystander effect (RIBE) has important implications for the efficiency of radiotherapy but the underlying role of cellular metabolism is widely unknown. The roles of synthesis of cytochrome c oxidase 2 (SCO2), a key effector for respiratory chain, and related signaling factors in α-particle...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2013.817706
更新日期:2013-12-01 00:00:00
abstract:PURPOSE:We computed probabilities to have multiple double-strand breaks (DSB), which are produced in DNA on a regional scale, and not in close vicinity, in volumes matching the size of DNA damage foci, of a large chromatin loop, and in the physical volume of DNA containing the HPRT (human hypoxanthine phosphoribosyltra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000802499212
更新日期:2008-11-01 00:00:00
abstract:PURPOSE:To study the influence of TGF-beta1 and TGF-beta-neutralizing antibodies on the clonogenic activity and terminal differentiation of rat lung fibroblasts following radiation exposure. MATERIAL AND METHODS:Early passage rat lung fibroblasts were used in this study. Colony formation assays were applied to determi...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138501
更新日期:2000-04-01 00:00:00
abstract:PURPOSE:To investigate physical and biophysical properties of proton tracks 1 keV-300 MeV using Monte Carlo track structure methods. MATERIALS AND METHODS:We present model calculations for cross sections and methods for simulations of full-slowing-down proton tracks. Protons and electrons were followed interaction-by-...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2010.518204
更新日期:2011-02-01 00:00:00
abstract:PURPOSE:In case of a large-scale radiation accident when hundreds of people may be exposed, it is important to distinguish the severely exposed individuals (> or =1 gray), who require early medical treatment, from those less exposed. The aim of our study was to develop a quick population triage method based on automate...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553000903264481
更新日期:2010-01-01 00:00:00
abstract::Survival of HT29 cells was measured after irradiation with single doses of X-rays (0.05-5 Gy) and neutrons (0.025-1.5 Gy), using a Dynamic Microscopic Imaging Processing Scanner (DMIPS) with which individual cells can be accurately located in tissue culture flasks, their positions recorded, and after an appropriate in...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314450831
更新日期:1993-05-01 00:00:00
abstract:PURPOSE:Since previous research found an increase in the rate of aneuploidies in human lymphocytes exposed to radiofrequencies, it seems important to perform further studies. The objective of this study was then to investigate whether the exposure to RF (radiofrequency) radiation similar to that emitted by mobile phone...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2011.542543
更新日期:2011-04-01 00:00:00
abstract::The effects of a low dose (0.1-20 mGy) preirradiation with X-rays followed by a higher dose (2 Gy) of the same radiation on the recovery of the genetic damage induced as dominant lethals in mature oocytes (stage 14) of different strains of Drosophila melanogaster were investigated. The response was shown to be depende...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414550501
更新日期:1994-04-01 00:00:00
abstract::Ionizing radiation induces both quantitative and qualitative changes in the lymphoid cells of both man and experimental animals, including inhibition of antibody responses. However, the cellular basis of this immunological lesion is not clear. In the present study, groups of mice were exposed to 2.0 Gy gamma-rays or s...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009514550531
更新日期:1995-04-01 00:00:00
abstract::A study has been made of the intrinsic radiosensitivity of peripheral blood lymphocytes from normal individuals. Cell survival following in vitro high (1.55 Gy min-1) and low (0.0098 Gy min-1) dose-rate irradiation was obtained for single lymphocyte samples from three individuals in six experiments. Despite wide inter...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009314551571
更新日期:1993-10-01 00:00:00
abstract:PURPOSE:To investigate the effect of gamma radiation-induced alterations in antioxidant defence of radioresistant Sf9 insect cells. MATERIALS AND METHODS:Sf9 cells were irradiated at doses ranging from 0.5-200 Gy. Lipid peroxidation and protein carbonylation levels were observed at 4 h post-exposure along with reduced...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553002.2015.1054958
更新日期:2015-01-01 00:00:00
abstract:PURPOSE:To resolve the difficulty in counting merged DNA damage foci in high-LET (linear energy transfer) ion-induced patterns. MATERIALS AND METHODS:The analysis of patterns of RIF (radiation-induced foci) produced by high-LET Fe and Ti ions were conducted by using a Monte Carlo model that combines the heavy ion trac...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.3109/09553001003717175
更新日期:2010-06-01 00:00:00
abstract::The chemical instability of DNA under physiological conditions requires that cells have highly developed processes for repairing stochastic single-strand damage. It is proposed here that provided ionizing-radiation-induced single-strand damage does not occur at a rate sufficient to perturb the dynamic steady state bet...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009114552421
更新日期:1991-10-01 00:00:00
abstract:PURPOSE:To investigate the role of tumour versus non-tumour factors in the specific response to serotonin (5-HT) of tumour-feeding arterioles (TFA). MATERIALS AND METHODS:Using mouse models of intra-vital microscopy, the response to topical administration of 5-HT was studied in arterioles feeding tumours: fibrosarcoma...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530098141519
更新日期:1998-09-01 00:00:00
abstract:PURPOSE:We aimed for a quantitative evaluation that justifies guidelines for evacuation which take into consideration both the human and economic costs. To the best of our knowledge, such an evaluation has not been performed yet. The present guidelines published by the International Atomic Energy Agency (IAEA) are prob...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2020.1779962
更新日期:2020-11-01 00:00:00
abstract:PURPOSE:To follow plasma Flt3-ligand (FL) concentrations in irradiated animals in order to evaluate it as an indicator of bone marrow damage for the management of accidental radiation-induced aplasia. MATERIALS AND METHODS:Non-human primates were irradiated at doses ranging from 2 to 8 Gy, using whole- or partial-body...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110043711
更新日期:2001-06-01 00:00:00
abstract::Single crystals of cytosine hydrochloride with thiocytosine as an impurity were found suitable for the study of a possible new mechanism of long-range migration of energy deposited by ionizing radiation. In a crystal containing thiocytosine, two kinds of chlorine-containing paramagnetic centres are present that are co...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009414550891
更新日期:1994-07-01 00:00:00
abstract:PURPOSE:To investigate the hypothesis that the developmental phase at which an individual encounters radiation damage affects its long-term sensitivity to a subsequent tumourigenic insult. MATERIALS AND METHODS:Either the pregnant C57B16 mouse was exposed to 137Cs gamma-rays at 4 or 15 days post-conception (embryonic ...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000139032
更新日期:2000-01-01 00:00:00
abstract:PURPOSE:Effects of radiation on the differentiation of C2C12 myoblasts were studied using cell cultures in which the cells differentiated into either muscle cells or osteoblasts. MATERIALS AND METHODS:A mouse skeletal myoblast cell line, C2C12, was cultured and irradiated at confluence. Muscle differentiation was esti...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530000138745
更新日期:2000-03-01 00:00:00
abstract::In the last decade the 'comet assay' or 'single cell gel electrophoresis assay' has been established as a sensitive method for the detection of DNA damage and the measurement of its recovery. The results published in the literature have often been obtained with different methods for comet structure measurement. In mos...
journal_title:International journal of radiation biology
pub_type:
doi:10.1080/095530097143220
更新日期:1997-10-01 00:00:00
abstract::Determinations of cell sensitivity in terms of survival fraction after doses employed in clinical radiation therapy, say 1-3 Gy, are of increasing interest to clinicians as they provide direct experimental data which can be employed without reference to models of cell inactivation. SF2 values are expected ultimately t...
journal_title:International journal of radiation biology
pub_type: 杂志文章,评审
doi:10.1080/09553008914551971
更新日期:1989-11-01 00:00:00
abstract:PURPOSE:To compare experimental yields of single strand breaks (SSB) and double strand breaks (DSB) induced in plasmid DNA in aqueous solution by alpha-particles and Al(K) ultrasoft X-rays (USX) with the corresponding yields, generated via computer simulations, for a range of mean diffusion distances of the hydroxyl ra...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553000110069308
更新日期:2001-10-01 00:00:00
abstract:PURPOSE:Gene therapy combined with radiation has shown promising potential for the treatment of tumors. This paper aimed to clarify the synergistic effect of radiotherapy combined with the bladder cancer tissue-specific oncolytic adenovirus (Ad-PSCAE-UPII-E1A) on bladder cancer cells and to study the underlying synergy...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553002.2017.1231942
更新日期:2017-02-01 00:00:00
abstract::Electron transfer to 5-bromouracil (5-BrU) from nucleobase (N) electron adducts (and their protonated forms) has been studied by product analysis and pulse radiolysis. When an electron is transferred to 5-BrU, the ensuing 5-BrU radical anion rapidly loses a bromide ion; the uracilyl radical thus formed reacts with add...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/09553009214552451
更新日期:1992-11-01 00:00:00
abstract::This study was designed to compare radiosensitization by the oral platinum compound JM216 with cisplatin. RIF1 mouse tumour cells were treated at various doses and at various exposure times with JM216 and irradiated 15 min before the end of drug exposure. The fraction of cells surviving treatment was assessed by colon...
journal_title:International journal of radiation biology
pub_type: 杂志文章
doi:10.1080/095530097142834
更新日期:1997-12-01 00:00:00
abstract:PURPOSE:To examine whether a simulated mobile telephone transmission at 915 MHz has an effect on cognitive function in man. MATERIALS AND METHODS:Thirty-six subjects in two groups were each given two training sessions and then three test sessions in a randomized three-way cross-over design. About 1 W mean power at 915...
journal_title:International journal of radiation biology
pub_type: 临床试验,杂志文章,随机对照试验
doi:10.1080/095530099140375
更新日期:1999-04-01 00:00:00