Abstract:
:Increases of individual beta tubulin isotypes in antimicrotubule drug resistant cell lines have been reported by several laboratories. We have previously described elevations in beta(III)and beta(IVa)isotypes in estramustine and paclitaxel resistant human prostate carcinoma cells. To investigate further the function of beta tubulin isotypes in antimicrotubule drug response, human prostate carcinoma cells that normally have very low to undetectable levels of beta(III)were stably transfected with beta(III)cDNA in pZeoSV system. An 18 bp haemagglutinin (HA) epitope tag was added at the 3' end prior to cloning into the vector. Cells were transfected with pZeoSV or pZeoSV-beta(III)plasmids and selected in the presence of Zeocin. Immunofluorescent staining of the transfectant cells have shown significant expression and incorporation of HA-tagged beta(III)tubulin into cellular microtubules. Quantitation of Western blots revealed the HA-tagged beta(III)levels to be approximately 7-fold higher than the vector control cells. RT-PCR analysis confirmed the increase at the transcript level and also revealed a collateral increase of beta(II)and beta(IVb)transcripts. Cell viability assays indicated that sensitivity of beta(III)transfected cells to various antimicrotubule agents was similar to vector transfected cells: IC50 values for estramustine, paclitaxel, colchicine and vinblastine were 4 microM, 4 nM, 22 nM and 2 nM, respectively for both cell lines. Thus, overexpression of beta(III)isotype in human prostate carcinoma cells by stable transfection failed to confer antimicrotubule drug resistance to these cells. Counterregulatory increases of endogenous beta(II)and beta(IVb)tubulin isotypes in these beta(III)transfected cells may be a compensatory mechanism used by the cells to overcome the effects of elevated beta(III)levels on the cellular microtubules. These results highlight the difficulty in isolating the contribution of single tubulin isotypes in drug response studies.
journal_name
Br J Cancerjournal_title
British journal of cancerauthors
Ranganathan S,McCauley RA,Dexter DW,Hudes GRdoi
10.1054/bjoc.2001.1956subject
Has Abstractpub_date
2001-09-01 00:00:00pages
735-40issue
5eissn
0007-0920issn
1532-1827pii
S0007092001919561journal_volume
85pub_type
杂志文章abstract::Tumorigenic mouse lung-derived type 2 cell lines have large reductions in both beta-adrenergic-stimulated cAMP production and ligand binding to beta-adrenergic receptors. These tumorigenic cells are also relatively insensitive to glucocorticoids. Because glucocorticoids regulate both beta-adrenergic receptor expressio...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1996.377
更新日期:1996-08-01 00:00:00
abstract:BACKGROUND:The Nottinghamshire Lymphoma Registry contains the details of all the patients diagnosed with lymphoma (since 1 January 1973) within a defined geographical area with a population of 1.1 million. It was therefore possible to study the outcome of treatment for Hodgkin's disease for three 10-year cohorts (1973-...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.228
更新日期:2012-07-24 00:00:00
abstract::Common warts could indicate cervical cancer susceptibility, as both are caused by human papillomavirus (HPV). Eczema was also investigated, as atopic eczema has been negatively associated with warts, but non-atopic eczema may be associated with compromised host defences, as observed in patients with HIV, suggesting in...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6600585
更新日期:2002-10-21 00:00:00
abstract::On the basis of preclinical data suggesting the possibility of maximising the efficacy of 5-fluorouracil and cisplatin by interferon, a pilot clinical trial was initiated in recurrent and/or metastatic head and neck cancer. Thirty-four patients were treated with cisplatin at 100 mg m-2, followed by 5-fluorouracil at 1...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/bjc.1994.72
更新日期:1994-02-01 00:00:00
abstract:BACKGROUND:There are currently two million cancer survivors in the United Kingdom, and in recent years this number has grown by 3% per annum. The aim of this paper is to provide long-term projections of cancer prevalence in the United Kingdom. METHODS:National cancer registry data for England were used to estimate can...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.366
更新日期:2012-09-25 00:00:00
abstract:BACKGROUND:Increased survival after cancer in young age has made long-term follow-up studies of high external validity important. In this national cohort study, we explored the impact of cancer in young age on reproduction and marital status in male survivors. METHODS:Hazard ratios (HRs) and relative risks (RRs) of re...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2015.455
更新日期:2016-02-02 00:00:00
abstract::The tolerability, anti-tumour activity and pharmacokinetic interaction of high-dose intravenous cyclosporin combined with intravenous etoposide was evaluated in children. Eighteen patients with recurrent or refractory tumours, all of whom had previously received etoposide, were treated with a combination of high-dose ...
journal_title:British journal of cancer
pub_type: 临床试验,杂志文章
doi:10.1038/bjc.1998.383
更新日期:1998-06-01 00:00:00
abstract::Male and female Swiss strain mice were put on a 0.03% thioacetamide diet at the age of 2 months. Control mice were kept on a stock diet. Control and treated mice were killed at the ages of 6, 9, 13 and 17 months. Progressive morphological, histological and biochemical alterations in the liver and tumour tissue of the ...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1970.60
更新日期:1970-09-01 00:00:00
abstract::We used the nationwide Swedish Family-Cancer Database with 2060 childhood brain tumours diagnosed in the period 1958-1996 to analyse the risk of this tumour by parental cancers and in siblings of childhood brain tumour probands. Groups of patients were compared by calculating standardized incidence ratios (SIRs) for b...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1054/bjoc.2000.1252
更新日期:2000-08-01 00:00:00
abstract::In this study, the immunohistochemical expression of a new inducible elastase inhibitor, SKALP (skin-derived anti-leucoproteinase)/elafin, in the tissue of squamous cell carcinoma and uninvolved oesophageal mucosa was studied using a polyclonal rabbit anti-serum against SKALP/elafin. The results were compared with the...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1997.511
更新日期:1997-01-01 00:00:00
abstract::The effects of local tumour radiation over the dose range 7.5-30 Gy on the growth and cell kinetics of rat hepatoma H-4-II-E have been investigated. A plot of growth delays against log surviving fraction was linear below a fraction of 0.03, but failed to extrapolate to the origin. Following a single dose of 15 Gy to t...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1980.283
更新日期:1980-10-01 00:00:00
abstract::Animal experiments remain essential to understand the fundamental mechanisms underpinning malignancy and to discover improved methods to prevent, diagnose and treat cancer. Excellent standards of animal care are fully consistent with the conduct of high quality cancer research. Here we provide updated guidelines on th...
journal_title:British journal of cancer
pub_type: 杂志文章,实务指引
doi:10.1038/sj.bjc.6605642
更新日期:2010-05-25 00:00:00
abstract::Soluble and nuclear oestrogen receptor status was determined in both the central and peripheral portions of tumour for 37 cases of adenocarcinoma of the endometrium. Of these, 29 had functional receptor in the peripheral biopsy, but only 19 retained functional receptor in the centre. Six of the 10 patients whose tumou...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1983.34
更新日期:1983-02-01 00:00:00
abstract::We investigated whether dietary intakes of total fat, monounsaturated fat (MUFA), polyunsaturated fat (PUFA) and saturated fat (SFA) were associated with breast cancer risk in a prospective cohort of 49 261 Swedish women (30-49 years at enrolment), which yielded 974 breast cancer cases by December 2005. Further, we ev...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6604033
更新日期:2007-12-03 00:00:00
abstract::We screened 145 HIV-infected non-pregnant women at a tertiary care centre in Lusaka, Zambia. Liquid-based cytology and human papillomavirus (HPV) genotyping with PGMY09/11 biotinylated primers (Roche Linear Array HPV genotyping test) maximised sensitivity of cytology and HPV assessments. Among high-risk (HR) types, HP...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6603737
更新日期:2007-05-07 00:00:00
abstract:BACKGROUND:The percentage of tumour stroma (TSP) has recently been reported to be a novel independent predictor of outcome in patients with a variety of common solid organ tumours. The aim of this study was to examine the relationship between TSP, clinicopathological characteristics and outcome in patients with invasiv...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2014.279
更新日期:2014-07-08 00:00:00
abstract::Ovulation is believed to contribute to the development of ovarian cancers that derive from the ovarian surface epithelium (OSE). The process of ovulation is synonymous with inflammation and inflammatory cytokines such as interleukin-1alpha (IL-1alpha) have recently been shown to induce both inflammatory and anti-infla...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602568
更新日期:2005-05-23 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1994.269
更新日期:1994-07-01 00:00:00
abstract::A total of 152 non-small cell lung cancers (NSCLC) were studied retrospectively to determine the relationship between epidermal growth factor receptor (EGF-R) status and the histological type, tumour size, nodal status and prognosis. EGF-R status was assessed on routinely embedded paraffin sections with an antibody to...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1989.156
更新日期:1989-05-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6602950
更新日期:2006-02-13 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/sj.bjc.6605544
更新日期:2010-03-02 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
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更新日期:1985-04-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2012.403
更新日期:2012-10-09 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2013.548
更新日期:2013-10-15 00:00:00
abstract:BACKGROUND:Bortezomib is a proteasome inhibitor with minimal clinical activity as a monotherapy in solid tumours, but its combination with other targeted therapies is being actively investigated as a way to increase its anticarcinogenic properties. Here, we evaluate the therapeutic potential of co-treatment with bortez...
journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.2011.546
更新日期:2012-01-17 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章,评审
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更新日期:2015-01-06 00:00:00
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journal_title:British journal of cancer
pub_type: 临床试验,杂志文章,多中心研究,随机对照试验
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更新日期:1997-01-01 00:00:00
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journal_title:British journal of cancer
pub_type: 杂志文章
doi:10.1038/bjc.1978.236
更新日期:1978-10-01 00:00:00
abstract::Major advances in cancer immunotherapy have dramatically expanded the potential to manipulate immune cells in cancer patients with metastatic disease to counteract cancer spread and extend patient lifespan. One of the most successful types of immunotherapy is the immune checkpoint inhibitors, such as anti-CTLA-4 and a...
journal_title:British journal of cancer
pub_type: 杂志文章,评审
doi:10.1038/s41416-020-01160-5
更新日期:2021-01-01 00:00:00
abstract:BACKGROUND:Some components of the Mediterranean diet have favourable effects on endometrial cancer, and the Mediterranean diet as a whole has been shown to have a beneficial role on various neoplasms. METHODS:We analysed this issue pooling data from three case-control studies carried out between 1983 and 2006 in vario...
journal_title:British journal of cancer
pub_type: 杂志文章,meta分析
doi:10.1038/bjc.2015.153
更新日期:2015-05-26 00:00:00