当前位置： SCI文献检索 > JOURNAL OF NEUROTRAUMA期刊下所有文献 > Small shifts in craniotomy position in the lateral fluid percussion injury model are associated with differential lesion development.

Small shifts in craniotomy position in the lateral fluid percussion injury model are associated with differential lesion development.

Abstract：

:Previous studies have shown that location and direction of injury may affect outcome in experimental models of traumatic brain injury. Significant variability in outcome data has also been noted in studies using the lateral fluid percussion brain injury model (FPI) in rats. In recent studies from our laboratory, we observed considerable variability in localization and severity of tissue damage as a function of small changes in craniotomy position. To further address this issue, we examined the relationship between craniotomy position and brain lesion size/location in rats subjected to moderate FPI (2.28 +/- 0.18 atmospheres). With placement of a 5-mm craniotomy adjacent to the sagittal suture, there was both ipsilateral and contralateral damage as detected at 3 weeks posttrauma using T2-weighted magnetic resonance imaging (MRI). The MRI lesions were generally restricted to the hippocampus and subcortical layers. Shifting of the craniotomy site laterally was associated with increased ipsilateral tissue damage and a greater cortical component that correlated with distance from the sagittal suture. In contrast, the contralateral MRI lesion did not change significantly in size or location unless the center of the craniotomy was placed more than 3.5 mm from the sagittal suture, under which condition contralateral damage could no longer be detected. Ipsilateral tissue damage as determined from the MRI scans was linearly correlated to motor outcome but not with cognitive outcome as assessed by the Morris Water Maze. We conclude that craniotomy position is critical in determining extent and location of tissue injury produced during the lateral FPI model in rats. Addressing such potential variability is essential for studies that address either injury mechanisms or therapeutic treatments.

journal_name

J Neurotrauma

journal_title

Journal of neurotrauma

authors

Vink R,Mullins PG,Temple MD,Bao W,Faden AI

doi

10.1089/089771501316919201

subject

Has Abstract

pub_date

2001-08-01 00:00:00

pages

839-47

issue

eissn

0897-7151

issn

1557-9042

journal_volume

pub_type

杂志文章

在线工具

Small shifts in craniotomy position in the lateral fluid percussion injury model are associated with differential lesion development.