Insertional mutagenesis of preneoplastic astrocytes by Moloney murine leukemia virus.

Abstract:

:Retroviral infection can induce transcriptional activation of genes flanking the sites of proviral integration in target cells. Because integration is essentially random, this phenomenon can be exploited for random mutagenesis of the genome, and analysis of integration sites in tumors may identify potential oncogenes. Here we have investigated this strategy in the context of astrocytoma progression. Neuroectodermal explants from astrocytoma-prone GFAP-v-src transgenic mice were infected with the ecotropic Moloney murine leukemia virus (Mo-MuLV). In situ hybridization and FACS analysis indicated that astrocytes from E12.5-13.5 embryos were highly susceptible to retroviral infection and expressed viral RNA and proteins both in vitro and in vivo. In average 80% of neuroectodermal cells were infected in vitro with 9-14 proviral integrations per cell. Virus mobility assays confirmed that Mo-MuLV remained transcriptionally active and replicating in neuroectodermal primary cultures even after 45 days of cultivation. Proviral insertion sites were investigated by inverse long-range PCR. Analysis of a limited number of provirus flanking sequences in clones originated from in vitro infected GFAP-v-src neuroectodermal cells identified loci of possible relevance to tumorigenesis. Therefore, the approach described here might be suitable for acceleration of tumorigenesis in preneoplastic astrocytes. We expect this method to be useful for identifying genes involved in astrocytoma development/progression in animal models.

journal_name

J Neurovirol

journal_title

Journal of neurovirology

authors

Afanasieva TA,Pekarik V,Grazia D'Angelo M,Klein MA,Voigtländer T,Stocking C,Aguzzi A

doi

10.1080/13550280152058834

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

169-81

issue

2

eissn

1355-0284

issn

1538-2443

journal_volume

7

pub_type

  • Nonhuman primate models of NeuroAIDS.

    abstract::Human Immunodeficiency virus (HIV), the virus that causes acquired immunodeficiency syndrome (AIDS), also manifests neurological complications. HIV-associated dementia (HAD) is the most severe form of HIV-induced neurocognitive disorders. HIV encephalitis (HIVE), the pathological correlate of HAD, is characterized by ...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章,评审

    doi:10.1080/13550280802074539

    authors: Williams R,Bokhari S,Silverstein P,Pinson D,Kumar A,Buch S

    更新日期:2008-08-01 00:00:00

  • Alzheimer's disease neuropathology may not predict functional impairment in HIV: a report of two individuals.

    abstract::With aging of HIV populations, there is concern that Alzheimer's disease (AD) may become prevalent and difficult to distinguish from HIV-associated neurocognitive disorders. To date, there are no reports documenting histologically verified Alzheimer's neuropathology in individuals with HIV and dementia. Herein, we rep...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1007/s13365-018-0663-z

    authors: Morgello S,Jacobs M,Murray J,Byrd D,Neibart E,Mintz L,Meloni G,Chon C,Crary J

    更新日期:2018-10-01 00:00:00

  • Diagnosis and clinical management of neurological disorders caused by cytomegalovirus in AIDS patients. European Union Concerted Action on Virus Meningitis and Encephalitis.

    abstract::Cytomegalovirus (CMV) infections are common and severe complications of HIV infection. The virus involves the nervous system, causing encephalitis, polyradiculomyelitis and peripheral neuropathies. Due to their limited sensitivity, traditional virological approaches, such as virus isolation or antigen detection in the...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章,评审

    doi:10.3109/13550289809113490

    authors: Cinque P,Cleator GM,Weber T,Monteyne P,Sindic C,Gerna G,van Loon AM,Klapper PE

    更新日期:1998-02-01 00:00:00

  • A review of the potential mechanisms of neuronal toxicity associated with antiretroviral drugs.

    abstract::Highly active antiretroviral treatment has led to unprecedented efficacy and tolerability in people living with HIV. This effect was also observed in the central nervous system with the nowadays uncommon observation of dementias; yet in more recent works milder forms are still reported in 20-30% of optimally treated i...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章,评审

    doi:10.1007/s13365-020-00874-9

    authors: De Benedetto I,Trunfio M,Guastamacchia G,Bonora S,Calcagno A

    更新日期:2020-10-01 00:00:00

  • Incidence of symptomatic CSF viral escape in HIV infected patients receiving atazanavir/ritonavir (ATV/r)-containing ART: a tertiary care cohort in western India.

    abstract::This single-center study attempts to quantify the incidence of symptomatic CSF viral escape (CSFVE) in patients receiving atazanavir/r (ATV/r)-containing regimen. We performed a retrospective analysis of patients receiving ATV/r-containing ART who were diagnosed with symptomatic CSFVE from August 2012 to January 2017....

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1007/s13365-018-0642-4

    authors: Patel AK,Patel KK,Gohel S,Kumar A,Letendre S

    更新日期:2018-08-01 00:00:00

  • Diagnosing acute encephalitis in patients with hematological disorders: caveats and pitfalls.

    abstract::The aim of this study was to review the quality of the diagnostic work-up for acute encephalitis carried out at our center in a cohort of patients with hematological disorders. Our data showed substantial heterogeneity in investigating patients. Not all patients had their CSF tested for viruses commonly responsible fo...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1007/s13365-019-00817-z

    authors: Vegezzi E,Berzero G,Barbetta D,Colombo AA,Borsani O,Bernasconi P,Compagno F,Zecca M,Campanini G,Simoncelli A,Paoletti M,Pichiecchio A,Baldanti F,Brunetti E,Marchioni E

    更新日期:2020-04-01 00:00:00

  • Reinvestigation of the role of the rabies virus glycoprotein in viral pathogenesis using a reverse genetics approach.

    abstract::The rabies virus glycoprotein (G) gene of the highly neuroinvasive and neurotropic strains SHBRV-18, CVS-N2c, and CVS-B2c was introduced into the non-neuroinvasive and less neurotropic SN-10 strain to provide further insight into the role of G in the pathogenesis of rabies. Phenotypic analyses of the recombinant virus...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.3109/13550280009018301

    authors: Morimoto K,Foley HD,McGettigan JP,Schnell MJ,Dietzschold B

    更新日期:2000-10-01 00:00:00

  • Cognitive behavioural impairment with irreversible sensorineural deafness as a complication of West Nile encephalitis.

    abstract::Despite common clinical features to suggest encephalitis, different viral encephalitides are known to have some specific clinical features, which if present, may suggest infection by a particular virus. West Nile viral (WNV) encephalitis has not been described with any specific diagnostic feature so far. In this conte...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1007/s13365-019-00733-2

    authors: Pradhan S,Anand S,Choudhury SS

    更新日期:2019-06-01 00:00:00

  • Effort and neuropsychological performance in HIV-infected individuals on stable combination antiretroviral therapy.

    abstract::The expression of cognitive symptoms associated with HIV varies over time and across individuals. This pattern may reflect transient contextual factors, including the degree of effort exerted by individuals undergoing cognitive testing. The present study examined whether effort corresponds to the expression of persist...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1007/s13365-017-0557-5

    authors: Paul R,Rhee G,Baker LM,Vaida F,Cooley SA,Ances BM

    更新日期:2017-10-01 00:00:00

  • Epstein-Barr virus DNA in cerebrospinal fluid from an immunocompetent man with herpes simplex virus encephalitis.

    abstract::Herpes simplex virus 1 meningo-encephalitis was ascertained in a 63-year-old immunocompetent man. To determine the duration of the persistence of herpesvirus DNA in the central nervous system, the cerebrospinal fluid was periodically monitored by polymerase chain reaction for 53 days. In addition to HSV-1, Epstein-Bar...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.3109/13550289809114547

    authors: Portolani M,Sabbatini AM,Meacci M,Pietrosemoli P,Cermelli C,Lunghi P,Golinelli F,Stacca R

    更新日期:1998-08-01 00:00:00

  • Restricted replication of herpes simplex virus in satellite glial cell cultures clonally derived from adult mice.

    abstract::To determine the possible influence of satellite glial cells on restricting the spread of herpes simplex virus in the peripheral nervous system, HSV replication was studied in clonally derived cultures of satellite glial cells from adult animals. Satellite cells were purified by exploiting their close anatomical assoc...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.3109/13550289909029479

    authors: Wilkinson R,Leaver C,Simmons A,Pereira RA

    更新日期:1999-08-01 00:00:00

  • Human cytomegalovirus induces IL-6 and TNF alpha from macrophages and microglial cells: possible role in neurotoxicity.

    abstract::Human cytomegalovirus (HCMV) can frequently infect the central nervous system (CNS) in the setting of immunosuppression such as transplantation and infection with the human immunodeficiency virus (HIV). Our laboratory previously reported that HCMV infection of human brain aggregates preferentially infected a microglia...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.3109/13550289509113968

    authors: Pulliam L,Moore D,West DC

    更新日期:1995-06-01 00:00:00

  • Human immunodeficiency virus-1 evolutionary patterns associated with pathogenic processes in the brain.

    abstract::The interplay between pathology and human immunodeficiency virus (HIV) expansion in brain tissues has not been thoroughly assessed in the highly active antiretroviral therapy (HAART) era. HIV-associated dementia (HAD) is marked by progressive brain infection due to recruitment and migration of macrophages in brain tis...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.3109/13550281003735709

    authors: Lamers SL,Salemi M,Galligan DC,Morris A,Gray R,Fogel G,Zhao L,McGrath MS

    更新日期:2010-05-01 00:00:00

  • Glycogen synthase kinase-3beta (GSK-3beta) inhibitors AR-A014418 and B6B3O prevent human immunodeficiency virus-mediated neurotoxicity in primary human neurons.

    abstract::Glycogen synthase kinase-3beta (GSK3beta) role in human immunodeficiency virus(HIV)-associated neurodegeneration has been evidenced by previous investigations. In this study, we investigated the specificity of two GSK3beta-specific inhibitors, AR-A014418 (A) and B6B30 (B) to prevent direct neurotoxicity in primary hum...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1080/13550280903168131

    authors: Nguyen TB,Lucero GR,Chana G,Hult BJ,Tatro ET,Masliah E,Grant I,Achim CL,Everall IP,HIV Neurobehavioral Research Group.

    更新日期:2009-09-01 00:00:00

  • Progressive multifocal leukoencephalopathy treated with nivolumab.

    abstract::Therapy for progressive multifocal leukoencephalopathy (PML) remains challenging since there are no antiviral therapies available for JC virus. Immune reconstitution has improved the prognosis in many settings where PML occurs, but it often is not possible in PML patients with hematologic malignancies. We describe the...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1007/s13365-019-00738-x

    authors: Hoang E,Bartlett NL,Goyal MS,Schmidt RE,Clifford DB

    更新日期:2019-04-01 00:00:00

  • CD4+ and CD8+ T cells are not major effectors of mouse hepatitis virus A59-induced demyelinating disease.

    abstract::We examined murine hepatitis virus strain A59 (MHV-A59)-induced demyelinating disease in C57BL/6 mice which had previously been thymectomized at 25 days of age. Demyelination was observed in 51-96% of spinal cord quadrants examined 30 or 60 days post infection (dpi), indicating that neither an intact thymus nor thymic...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.3109/13550289709018297

    authors: Sutherland RM,Chua MM,Lavi E,Weiss SR,Paterson Y

    更新日期:1997-06-01 00:00:00

  • Ventral striatal and septal area hypermetabolism on FDG-PET in herpes simplex viral encephalitis.

    abstract::A 71-year-old man presented with sudden onset, generalized tonic-clonic seizures and altered mental status. Initial brain magnetic resonance imaging was normal but a brain FDG-PET scan showed hypermetabolism in the left ventral striatum and septal area. Initial cerebrospinal fluid (CSF) examination showed mildly eleva...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1007/s13365-019-00779-2

    authors: Singhal T,Solomon I,Akbik F,Smirnakis S,Vaitkevicius H

    更新日期:2020-02-01 00:00:00

  • Age exacerbates HIV-associated white matter abnormalities.

    abstract::Both HIV disease and advanced age have been associated with alterations to cerebral white matter, as measured with white matter hyperintensities (WMH) on fluid-attenuated inversion recovery (FLAIR) magnetic resonance imaging (MRI), and more recently with diffusion tensor imaging (DTI). This study investigates the comb...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1007/s13365-015-0386-3

    authors: Seider TR,Gongvatana A,Woods AJ,Chen H,Porges EC,Cummings T,Correia S,Tashima K,Cohen RA

    更新日期:2016-04-01 00:00:00

  • HIV eradication symposium: will the brain be left behind?

    abstract::On 18 July 2014, the National Institute of Mental Health in collaboration with ViiV Health Care and Boehringer Ingelheim supported a symposium on HIV eradication and what it meant for the brain. The symposium was an affiliated event to the 20th International AIDS Conference. The meeting was held in Melbourne, Australi...

    journal_title:Journal of neurovirology

    pub_type:

    doi:10.1007/s13365-015-0322-6

    authors: Brew BJ,Robertson K,Wright EJ,Churchill M,Crowe SM,Cysique LA,Deeks S,Garcia JV,Gelman B,Gray LR,Johnson T,Joseph J,Margolis DM,Mankowski JL,Spencer B

    更新日期:2015-06-01 00:00:00

  • Epstein-Barr virus encephalitis presenting with a tumor-like lesion in an immunosuppressed transplant recipient.

    abstract::Epstein-Barr virus (EBV) associated central nervous system (CNS) infection is a rare disease. We report an atypical manifestation of EBV encephalitis initially presenting with a tumor-like lesion of the optic tract in an immunocompromised patient 8 years after a combined kidney and pancreas transplantation had been pe...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1080/13550280802345715

    authors: Khalil M,Enzinger C,Wallner-Blazek M,Scarpatetti M,Barth A,Horn S,Reiter G

    更新日期:2008-11-01 00:00:00

  • Human encephalitis caused by pseudorabies virus infection: a case report.

    abstract::Pseudorabies virus (PRV) primarily infects swine but can infect cattle, dogs, and cats. Several studies have reported that PRV can cross the specie barrier and induce human encephalitis, but a definitive diagnosis of human PRV encephalitis is debatable due to the lack of PRV DNA detection. Here, we report a case of hu...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1007/s13365-019-00822-2

    authors: Wang D,Tao X,Fei M,Chen J,Guo W,Li P,Wang J

    更新日期:2020-06-01 00:00:00

  • The "Three Italy" of the COVID-19 epidemic and the possible involvement of SARS-CoV-2 in triggering complications other than pneumonia.

    abstract::Coronavirus disease 2019 (COVID-19), first reported in Wuhan, the capital of Hubei, China, has been associated to a novel coronavirus, the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). In March 2020, the World Health Organization declared the SARS-CoV-2 infection a global pandemic. Soon after, the numb...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章,评审

    doi:10.1007/s13365-020-00862-z

    authors: Prezioso C,Marcocci ME,Palamara AT,De Chiara G,Pietropaolo V

    更新日期:2020-06-01 00:00:00

  • The role of depression chronicity and recurrence on neurocognitive dysfunctions in HIV-infected adults.

    abstract::Research assessing whether major depressive disorders (MDD) impacts neurocognitive functions in HIV+ persons has yielded inconsistent results. However, none have considered the role of MDD remission, chronicity, and stability on treatment. Ninety-five HIV+ adults clinically stable on combined antiretroviral treatment ...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1007/s13365-015-0368-5

    authors: Cysique LA,Dermody N,Carr A,Brew BJ,Teesson M

    更新日期:2016-02-01 00:00:00

  • Progressive multifocal leukoencephalopathy in Zambia is caused by JC virus with prototype regulatory region.

    abstract::There are only few documented cases of progressive multifocal leukoencephalopathy (PML) in Africa. Whether this is caused by a lack of JC virus (JCV) spread or alteration in the JCV genome is unknown. We characterized the clinical presentation, laboratory findings, and JCV regulatory region (RR) pattern of the first d...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1007/s13365-019-00746-x

    authors: Mubanga E,Patel A,Siddiqi OK,Hanson BA,Dang X,Lakhi S,Mulundu G,Bender N,Koralnik IJ

    更新日期:2019-08-01 00:00:00

  • Inhibition of nitric oxide synthase-2 reduces the severity of mouse hepatitis virus-induced demyelination: implications for NOS2/NO regulation of chemokine expression and inflammation.

    abstract::Infection of C57BL/6 mice with mouse hepatitis virus strain V5A13.1 (MHV-V5A13.1) results in an acute encephalitis followed by a chronic, progressive demyelinating disease with clinical and histological similarities to the human demyelinating disease Multiple Sclerosis (MS). Studies were undertaken to evaluate the con...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.3109/13550289909029745

    authors: Lane TE,Fox HS,Buchmeier MJ

    更新日期:1999-02-01 00:00:00

  • Human polyomavirus JC reactivation and pathogenetic mechanisms of progressive multifocal leukoencephalopathy and cancer in the era of monoclonal antibody therapies.

    abstract::Progressive multifocal leukoencephalopathy (PML) is a demyelinating disease of the central nervous system caused by the neurotropic human polyomavirus JC (JCV) lytic infection of oligodendrocytes. PML was first described as a complication of lymphoproliferative disorders more than 50 years ago and emerged as a major c...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章,评审

    doi:10.1007/s13365-012-0080-7

    authors: Bellizzi A,Nardis C,Anzivino E,Rodìo D,Fioriti D,Mischitelli M,Chiarini F,Pietropaolo V

    更新日期:2012-02-01 00:00:00

  • Regulation of caspase 8- and caspase 9-induced apoptosis by the herpes simplex virus type 1 latency-associated transcript.

    abstract::The latency-associated transcript (LAT) is the only herpes simplex virus type 1 (HSV-1) gene that is abundantly transcribed during latency. Plasmids expressing LAT inhibit apoptosis induced by etoposide and ceramide in transiently transfected cells. LAT also inhibits apoptosis in trigeminal ganglia of rabbits and prom...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1080/13550280290101085

    authors: Henderson G,Peng W,Jin L,Perng GC,Nesburn AB,Wechsler SL,Jones C

    更新日期:2002-12-01 00:00:00

  • Efficient infection of brain microvascular endothelial cells by an in vivo-selected neuroinvasive SIVmac variant.

    abstract::A neuroinvasive/neuropathogenic SIV variant termed SIVmac182 was previously isolated and characterized (Watry et al, 1994). This neuroinvasive strain was derived from the uncloned strain SIVmac251 through serial animal passage of infected microglia, unlike previously reported neurovirulent strains. Importantly, the vi...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.3109/13550289809114528

    authors: Strelow LI,Watry DD,Fox HS,Nelson JA

    更新日期:1998-06-01 00:00:00

  • Cellular localization of human herpesvirus-6 in the brains of children with AIDS encephalopathy.

    abstract::Human herpesvirus-6, the etiologic agent of exanthem subitum, is a ubiquitous virus that infects almost all children by the age of 2 years and that has previously been shown to be neuroinvasive. These characteristics suggest that human herpesvirus-6 may be important in the neuropathogenesis of acquired immune deficien...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.3109/13550289509111008

    authors: Saito Y,Sharer LR,Dewhurst S,Blumberg BM,Hall CB,Epstein LG

    更新日期:1995-03-01 00:00:00

  • The recruitment of peripheral blood leukocytes to the brain is delayed in susceptible BALB/c compared to resistant C57BL/6 mice during herpes simplex virus encephalitis.

    abstract::The cerebral immune response induced by herpes simplex virus (HSV) encephalitis (HSE) was evaluated in susceptible BALB/c and resistant C57BL/6 mice. BALB/c and C57BL/6 (named C57BL/6-high) mice were respectively infected intranasally with 1 × 103 and 5 × 105 plaque-forming units (PFUs) of HSV-1. C57BL/6 mice (named C...

    journal_title:Journal of neurovirology

    pub_type: 杂志文章

    doi:10.1007/s13365-019-00730-5

    authors: Canivet C,Uyar O,Rhéaume C,Piret J,Boivin G

    更新日期:2019-06-01 00:00:00