Abstract:
:To explore the possible influence of defined genetic backgrounds on photoreceptor viability and function in mice carrying a targeted disruption of the rhodopsin gene, the severities of retinopathies in Rho-/- mice on C57BL/6J and 129Sv congenic backgrounds were compared by light microscopy and electroretinography and qualitatively by in situ end labeling of DNA in apoptotic photoreceptor nuclei of retinal sections. Cone photoreceptor viability and function were shown to deteriorate more slowly on the C57BL/6J background in comparison to that of the 129Sv, with significantly greater numbers of outer nuclear layer nuclei in the retinas of C57BL/6J mice at 3 and 4 months of age. Both amplitude and waveform features of the ERG were shown to be remarkably different in the two strains, indicating an approximately 6-fold difference in C57BL/6J Rho-/- mice compared to 129Sv Rho-/- mice at 80 days. Thus, in comparison with the 129Sv strain, genetic modifiers appear to constitute a component of the C57BL/6J background, the expression of which significantly protects cone photoreceptors from apoptotic death in a mutation-induced murine retinopathy. The differences in phenotype revealed in this study are sufficient in principle to provide a basis for comparisons to be made between QTLs in light-induced and mutation-induced systems.
journal_name
Vis Neuroscijournal_title
Visual neuroscienceauthors
Humphries MM,Kiang S,McNally N,Donovan MA,Sieving PA,Bush RA,Machida S,Cotter T,Hobson A,Farrar J,Humphries P,Kenna Pdoi
10.1017/s0952523801183100subject
Has Abstractpub_date
2001-05-01 00:00:00pages
437-43issue
3eissn
0952-5238issn
1469-8714pii
S0952523801183100journal_volume
18pub_type
杂志文章abstract::The visual response to a flash given in the dark is known to saturate according to the Michaelis-Menten relationship. Nevertheless, the incremental response from increasing levels of mean luminance tends to follow a Weber-Fechner relationship well into the saturation range determined from the Michaelis-Menten results....
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523800006593
更新日期:1991-04-01 00:00:00
abstract::GABA is a major inhibitory neurotransmitter in the mammalian retina and it acts at many different sites via a variety of postsynaptic receptors. These include GABAA receptors and bicuculline-resistant GABAC receptors. The release of acetylcholine (ACh) is inhibited by GABA and strongly potentiated by GABA antagonists....
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523800011652
更新日期:1997-09-01 00:00:00
abstract::The Food and Drug Administration (FDA) is responsible for determining whether medical device manufacturers have provided reasonable assurance, based on valid scientific evidence, that new devices are safe and effective for their intended use before they are introduced into the U.S. market. Most existing color vision d...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523804213256
更新日期:2004-05-01 00:00:00
abstract::Using autoradiographic and biochemical techniques, we studied the sodium-dependent forward and reverse transport of the neurotransmitter histamine in an arthropod photoreceptor in order to test whether the transporter plays a central role in visual signal transfer at this synapse. In particular, we asked whether the h...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523802193084
更新日期:2002-05-01 00:00:00
abstract::Regeneration of the teleost retina following surgical extirpation of 25% to 100% of the neural retina was investigated in goldfish (Carrasius auratus) and sunfish (Lepomis cyanellus). The retina will regenerate following removal of up to 95% of the neural retina, however complete extirpation prevented regeneration. Vi...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/S0952523807070265
更新日期:2007-05-01 00:00:00
abstract::The retinas of adult teleost fish can regenerate following injury, but little is known about the neuronal integration of the visual scene that is performed by the regenerated retina. Using goldfish retinal ganglion cells (RGCs) as the experimental system, an evaluation of dendritic arbor structure and passive electrot...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523899162060
更新日期:1999-03-01 00:00:00
abstract::Extrastriate areas TEO and V4 have been associated with form and color vision. Area V4 has also been suggested to participate in processes concerned with attention, stimulus salience, and perceptual learning. In a continuing effort to elucidate the connectional interactions and microcircuitry of these areas, we descri...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523800002480
更新日期:1994-05-01 00:00:00
abstract::As a part of ongoing efforts to understand the cholinergic circuitry in the mammalian retina, we studied the coexpression of nicotinic acetylcholine receptors (nAChRs) and gamma-aminobutyric acid (GABA), the GABA transporter 1 (GAT-1), or choline acetyltransferase (ChAT) immunoreactivity in the rabbit retina. Double-l...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523801181083
更新日期:2001-01-01 00:00:00
abstract::It is widely accepted that human color vision is based on two types of cone-opponent mechanism, one differencing L and M cone types (loosely termed "red-green"), and the other differencing S with the L and M cones (loosely termed "blue-yellow"). The traditional view of the early processing of human color vision sugges...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523801181125
更新日期:2001-01-01 00:00:00
abstract::Receptive field organization of cone-driven bipolar cells was investigated by intracellular recording in the intact light-adapted retina of the tiger salamander (Ambystoma tigrinum). Centered spots and concentric annuli of optimum dimensions were used to selectively stimulate the receptive field center and surround wi...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/S0952523811000071
更新日期:2011-05-01 00:00:00
abstract::The Tennessee Mouse Genome Consortium (TMGC) is in its fifth year of a ethylnitrosourea (ENU)-based mutagenesis screen to detect recessive mutations that affect the eye and brain. Each pedigree is tested by various phenotyping domains including the eye, neurohistology, behavior, aging, ethanol, drug, social behavior, ...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/S0952523805225087
更新日期:2005-09-01 00:00:00
abstract::We examined the effects of picrotoxin and pentylenetetrazol (PTZ) on the responses to motions of ON-OFF directionally selective (DS) ganglion cells of the turtle's retina. These drugs are antagonists of the inhibitory neurotransmitter GABA. For continuous motions, picrotoxin markedly reduced the overall directionality...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523800008105
更新日期:1996-05-01 00:00:00
abstract::To gain insight into how vision guides eye movements, monkeys were trained to make a single saccade to a specified target stimulus during feature and conjunction search with stimuli discriminated by color and shape. Monkeys performed both tasks at levels well above chance. The latencies of saccades to the target in co...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523899161042
更新日期:1999-01-01 00:00:00
abstract::The retinae of insectivores have been rarely studied, and their photoreceptor arrangements and expression patterns of visual pigments are largely unknown. We have determined the presence and distribution of cones in three species of shrews (common shrew Sorex araneus, greater white-toothed shrew Crocidura russula, dar...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523800176138
更新日期:2000-11-01 00:00:00
abstract:BACKGROUND:The human retinal pigment epithelium (RPE) is reportedly 3% bi-nucleated. The importance to human vision of multi-nucleated (MN)-RPE cells could be clarified with more data about their distribution in central retina. METHODS:Nineteen human RPE-flatmounts (9 ≤ 51 years, 10 > 80 years) were imaged at 12 locat...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/S0952523815000310
更新日期:2016-01-01 00:00:00
abstract::This study used the swept spatial-frequency method to compare retinal and cortical acuity in anesthetized young adult rhesus monkeys. Visual evoked potentials (VEPs) and pattern electroretinographic responses (PERGs) were recorded from 25 monkeys (age range: 4-12 years) anesthetized with a continuous infusion of propo...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523899164010
更新日期:1999-07-01 00:00:00
abstract::Synaptically localized calcium channels shape the timecourse of synaptic release, are a prominent site for neuromodulation, and have been implicated in genetic disease. In retina, it is well established that L-type calcium channels play a major role in mediating release of glutamate from the photoreceptors and bipolar...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/S0952523804214055
更新日期:2004-07-01 00:00:00
abstract::We tested the hypothesis that gamma-aminobutyric acid (GABA) is the transmitter released by barnacle photoreceptors onto postsynaptic cells (I-cells). GABA was applied to I-cells either by superfusion or by ejecting it with pressure from a pipette positioned close to the I-cell's soma. The I-cell's response to GABA wa...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523800005496
更新日期:1989-10-01 00:00:00
abstract::Anterograde transport of cholera toxin subunit B (CTb) was used to study the retinal projections in birds, with an emphasis on retinohypothalamic connections. Pigeons (Columbia livia) were deeply anesthetized and received unilateral intraocular injections of CTb. In addition to known contralateral retinorecipient regi...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523800002376
更新日期:1994-05-01 00:00:00
abstract::Saccadic suppression is the reduced visibility that occurs during saccadic eye movements. Recent psychophysical studies have suggested that this is due to a reduction in responsiveness of magnocellular (M), but not parvocellular (P), cells of the lateral geniculate nucleus. To address this and other phenomena of respo...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523801182106
更新日期:2001-03-01 00:00:00
abstract::Retinae of the crab Leptograpsus which had been maintained on a 12-h light/12-h dark cycle were cultured in vitro and exposed to 1 microM okadaic acid (OKA) at 0.75 h before light onset. Control retinae were subjected to the same routine and sampled at the same times without OKA treatment. At the concentration used, O...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523800010920
更新日期:1991-07-01 00:00:00
abstract::Eye growth and refraction are regulated by visual processing in the retina. Until now, the messengers released by the retina to induce these changes are largely unknown. Previously, it was found that glucagon amacrine cells respond to defocus in the retinal image and even to its sign. The expression of the immediate-e...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523802196064
更新日期:2002-11-01 00:00:00
abstract::Circadian rhythms affect the anatomy, physiology, and biochemistry of the visual cells in the eyes of the horseshoe crab (Limulus polyphemus). These rhythms are mediated by the activity of efferent neurons that project from the central nervous system to all of the eyes. In this study, the optic nerves of Limulus were ...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523800001334
更新日期:1991-05-01 00:00:00
abstract::The recent isolation of at least five different cDNAs encoding functional subunits of glutamate receptors (GluR1 to GluR5) has revealed a diversity whose function is not understood. To learn more about how these different receptor subunits are used in the brain, we undertook an in situ hybridization study of the retin...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523800006489
更新日期:1992-01-01 00:00:00
abstract::Increment threshold functions of the electroretinogram (ERG) b-wave were obtained from goldfish using an in vivo preparation to study intraretinal mechanisms underlying the increase in perceived brightness induced by depletion of retinal dopamine by 6-hydroxydopamine (6-OHDA). Goldfish received unilateral intraocular ...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s095252380000300x
更新日期:1994-07-01 00:00:00
abstract::Two groups of retinal cone bipolar cells (CBCs) in rats were found to express voltage-gated Na+ channels. The axon terminals of the first group stratify in sublamina 2 of the inner plexiform layer (IPL) and partially overlap with the OFF-cholinergic band. This group was identified as type 3 CBCs. The axon terminals of...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/S0952523808080851
更新日期:2008-09-01 00:00:00
abstract::The multi-input technique of Sutter and Tran (1992) yields multiple focal ERGs. The purpose here was to compare the components of this multifocal ERG to the components of the standard, full-field ERG. To record multifocal ERGs, an array of 103 hexagons was displayed on a monitor. Full-field (Ganzfeld) ERGs were elicit...
journal_title:Visual neuroscience
pub_type: 临床试验,杂志文章
doi:10.1017/s0952523800012190
更新日期:1997-05-01 00:00:00
abstract::Dopaminergic interplexiform cells in the retina of the blue acara were investigated using an antiserum against tyrosine hydroxylase and PAP visualization. In whole-mount preparations, we observed a homogeneous distribution of cell bodies throughout the retina without any indication of regional specialization. At the f...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523800010737
更新日期:1992-09-01 00:00:00
abstract::The effects of dopamine on luminosity-type horizontal cells have been documented in different vertebrate retinas, both in vivo and in vitro. Some of these effects may reflect direct action of dopamine onto these cells, but indirect effects mediated by presynaptic neurons cannot be ruled out. Furthermore, direct effect...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523800008312
更新日期:1995-05-01 00:00:00
abstract::The distribution of GABA-like immunoreactivity in the pigeon visual system was studied during the first 9 days after hatching using a mouse monoclonal antibody, mAb 3A12, to glutaraldehyde linked GABA (Matute & Streit, 1986). GABA-like immunoreactivity was seen in cell bodies as well as in neuropil at the level of bot...
journal_title:Visual neuroscience
pub_type: 杂志文章
doi:10.1017/s0952523800009846
更新日期:1989-12-01 00:00:00