Abstract:
:Biostimulatory effect of cell proliferation and bone formation by laser irradiation has been reported, however, very little is known about the molecular basis of mechanisms. We previously constructed the cDNA library of mouse osteoblastic cells (MC3T3-E1) which enhanced gene expression by laser irradiation using a subtracted gene cloning procedure. In the present study, we focused on a gene clone, designated as MCL-140, which exhibited the high homology of DNA sequence with mouse minichromosome maintenance (MCM) 3 gene. MCM3 is involved in the initiation of DNA replication as licensing factor in eukaryotic cells. Nucleotide sequence of MCL-140 insert was determined and assessed in the nucleic acid databases. The transcription level of MCL-140 was examined by Northern blot analysis. The DNA sequences of clone MCL-140 insert exhibited 96.2% homology with MCM 3 gene coding P1 protein. Higher MCM3 mRNA levels were observed in laser-irradiated cells compared to the levels in non-irradiated cells: furthermore, radiolabelled thymidine incorporation was increased by laser irradiation. These findings suggest that low-level laser irradiation may enhance DNA replication and play a role in stimulating proliferation of osteoblast through the enhancement of the MCM3 gene expression.
journal_name
Lasers Med Scijournal_title
Lasers in medical scienceauthors
Yamamoto M,Tamura K,Hiratsuka K,Abiko Ydoi
10.1007/pl00011357subject
Has Abstractpub_date
2001-01-01 00:00:00pages
213-7issue
3eissn
0268-8921issn
1435-604Xjournal_volume
16pub_type
杂志文章abstract::The purpose of this article was to analyze the photobiomodulator role of low-level laser therapy (LLLT) on the skeletal muscle remodeling following cryoinjury in rats, focusing the types I and III collagen proteins. Laser phototherapy has been employed to stimulate repair in different tissues. However, its role in ske...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-011-0951-9
更新日期:2011-11-01 00:00:00
abstract::Onychomycosis is a fungal infection of the nail. The aim of this randomized controlled clinical trial was to compare the efficacy of 2940-nm Er:YAG laser treatment combined with a 5% amorolfine lacquer versus amorolfine monotherapy for treating onychomycosis. In this study, patients with onychomycosis of the great toe...
journal_title:Lasers in medical science
pub_type: 杂志文章,随机对照试验
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abstract::Inflammation of synovial membrane and degeneration of articular cartilage in osteoarthritis (OA) lead to major changes in joint space width (JSW) and biochemical components such as collagen-II telopeptide (CTX-II) and matrix metallo protineases (MMP-3, 8, and 13). Low-level laser therapy (LLLT) is thought to have an a...
journal_title:Lasers in medical science
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更新日期:2017-02-01 00:00:00
abstract::Transoral laser microsurgery (TLM) is an emerging technique for the management of laryngeal and other head and neck malignancies. It is increasingly being used in place of traditional open surgery because of lower morbidity and improved organ preservation. Since the surgery is performed from the inside working outward...
journal_title:Lasers in medical science
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abstract::Nonablative skin rejuvenation using laser, intense pulsed lights (IPLs), or radiofrequency techniques are becoming increasingly popular. In this paper, a novel protocol that integrates IPL sessions, low intense light and vitamin C, low-weight hyaluronic acid, betaglucan dermal injection versus IPL photorejuvenation as...
journal_title:Lasers in medical science
pub_type: 临床试验,杂志文章,随机对照试验
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abstract::Photodynamic rejuvenation therapy (PDRT) is a growing field in cosmetic dermatology. In this study, different sources of light (a yellow laser, a red laser and ultraviolet A (UVA) lamps) were used to activate a second-generation photosensitiser, hypericin. Uptake of hypericin was monitored over 24 h and efficacy of PD...
journal_title:Lasers in medical science
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journal_title:Lasers in medical science
pub_type: 杂志文章
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journal_title:Lasers in medical science
pub_type: 杂志文章
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更新日期:2018-07-01 00:00:00
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journal_title:Lasers in medical science
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更新日期:2010-03-01 00:00:00
abstract::Despite the positive effects of low-level laser therapy (LLLT) on muscle fatigue before exercises using a single muscle group, the acute effects of LLLT on performance in cardiopulmonary exercise testing (CPET) are poorly understood. We aimed to assess the acute effects of LLLT on physiologic and electromyographic res...
journal_title:Lasers in medical science
pub_type: 杂志文章,随机对照试验
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更新日期:2014-11-01 00:00:00
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journal_title:Lasers in medical science
pub_type: 杂志文章
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journal_title:Lasers in medical science
pub_type: 杂志文章,评审
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-011-1040-9
更新日期:2012-09-01 00:00:00
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journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-011-0975-1
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abstract::The optical properties of hemoglobin could indicate the degree of hemolysis. We aimed to utilize this to develop a real-time blood damage monitoring device for cardiopulmonary bypass (CPB) systems. The real-time blood damage monitoring device comprised a near-infrared spectroscopy optical module with a fiber spectrome...
journal_title:Lasers in medical science
pub_type: 杂志文章
doi:10.1007/s10103-020-03094-z
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journal_title:Lasers in medical science
pub_type: 杂志文章
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
pub_type: 杂志文章,已发布勘误
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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journal_title:Lasers in medical science
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更新日期:2013-01-01 00:00:00
abstract::Use of the CO(2) laser (λ = 10.6 μm, continuous wave, defocused) is an established procedure for the treatment of premalignant lesions. Through employment of the sp-mode as well as scanners, thermal laser effects can be reduced but, on the other hand, a lesser degree of destruction of dysplastic cells could lead to an...
journal_title:Lasers in medical science
pub_type: 临床试验,杂志文章
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更新日期:2012-01-01 00:00:00
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