Abstract:
:In Escherichia coli, endonuclease III (endo III) repairs the oxidation products of 8-OHGua. However, the corresponding repair enzymes in eukaryotes have not been identified. Here we report that 8-hydroxyguanine (8-OHGua) is highly sensitive to further oxidation. We also show that Ntg2, a functional homolog of endo III in Saccharomyces cerevisiae, is capable of nicking the irradiated duplex DNA containing 8-OHGua. Moreover, Ntg2 formed a stable complex with the DNA upon incubation with NaBH(4). In contrast, Ntg1, another functional homolog of endo III, showed no such activities. These findings indicate that Ntg2 is, at least in part, responsible for repairing the oxidation products of 8-OHGua in eukaryotic cells.
journal_name
Biochem Biophys Res Communjournal_title
Biochemical and biophysical research communicationsauthors
Kim JE,You HJ,Choi JY,Doetsch PW,Kim JS,Chung MHdoi
10.1006/bbrc.2001.5305subject
Has Abstractpub_date
2001-08-03 00:00:00pages
1186-91issue
5eissn
0006-291Xissn
1090-2104pii
S0006-291X(01)95305-7journal_volume
285pub_type
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