Abstract:
:The mechanisms of the antineoplastic effect of nonsteroidal anti-inflammatory drugs (NSAIDs) still are unknown, but the induction of apoptosis is one of the possible mechanisms. We attempted to demonstrate the role of mitogen-activated protein (MAP) kinases, generally considered to be important mediators of proliferative and apoptotic signals, in NSAID-induced colon cancer cell apoptosis. Apoptosis was detected by demonstration of DNA fragmentation in agarose gel electrophoresis. Cell death was assessed by trypan blue dye exclusion method. MAP kinase activation was assessed by Western blot using phosphospecific antibodies to MAP kinases. Kinase assay using activating transcription factor-2 (ATF-2) fusion protein as a substrate was also performed for measuring p38 MAP kinase activity. For the inhibition of p38 MAP kinase, pyridinylimidazole compound (SB203580) was utilized. Caspase-3 activity was measured using the tetrapeptide fluorogenic substrate Ac-DEVD-AMC. Treatment of HT-29 cells with NSAIDs results in time- and dose-dependent induction of apoptosis, accompanied by sustained activation of all three MAP kinase subfamilies. The SB203580, a p38 MAP kinase inhibitor, reduced indomethacin-induced cell death by 43%, while PD098059, a MAPK/ERK kinase (MEK)1 inhibitor, did not affect cell death. p38 MAP kinase and caspase-3 activation were not significantly interlinked in indomethacin-induced apoptosis. From these results, we conclude that NSAIDs can induce prolonged activation of MAP kinases in colon cancer cells and that, of these, p38 MAP kinase may play a partial but significant role in indomethacin-induced apoptosis.
journal_name
Int J Colorectal Disjournal_title
International journal of colorectal diseaseauthors
Kim TI,Jin SH,Kim WH,Kang EH,Choi KY,Kim HJ,Shin SK,Kang JKdoi
10.1007/s003840100301subject
Has Abstractpub_date
2001-06-01 00:00:00pages
167-73issue
3eissn
0179-1958issn
1432-1262journal_volume
16pub_type
杂志文章abstract::Division of the inferior mesenteric artery flush with the aorta (high tie) allows a tension-free anastomosis in distal colorectal resections but may also diminish the blood supply. Tissue oxygen tension was measured proximal to the resection margin before and after either low or high division of the inferior mesenteri...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/BF00337583
更新日期:1995-01-01 00:00:00
abstract:PURPOSE:The effects of ulcerative colitis (UC) duration on biomarker accuracy are unknown. We investigated the effects of UC duration on the predictive accuracy of biomarkers including immunochemical fecal occult blood test (FOBT, also known as FIT), prostaglandin E-major urinary metabolite (PGE-MUM), and C-reactive pr...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-020-03636-5
更新日期:2020-09-01 00:00:00
abstract::Continence following the gracilis stimulated neosphincter reconstruction after total rectal excision is inferior to that obtained in the presence of an intact anal canal. We describe a modification of the alpha loop in which the tendon is brought through the belly of the gracilis muscle. The results in three patients ...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/BF00292246
更新日期:1994-01-01 00:00:00
abstract:PURPOSE:Chemical sphincterotomy (CS) is routinely applied in order to avoid the irreversible anal incontinence associated with the surgical treatment of chronic anal fissure (CAF). However, CS has a lower cure rate than surgery. We developed a screening test (using anal manometry) to separate those patients that are un...
journal_title:International journal of colorectal disease
pub_type: 临床试验,杂志文章
doi:10.1007/s00384-010-0885-x
更新日期:2010-05-01 00:00:00
abstract::Hysterectomy is associated with severe constipation in a subgroup of patients, and an adverse effect on colonic motility has been described in the literature. The onset of irritable bowel syndrome and urinary bladder dysfunction has also been reported after hysterectomy. In this prospective study, we investigated the ...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s003840050147
更新日期:1998-01-01 00:00:00
abstract:AIM:This study aims to evaluate the prognostic value of CD45RO(+) tumor-infiltrating lymphocytes (TILs) in locally advanced rectal cancer treated with 30 Gy/10 fraction (10 f) neoadjuvant radiotherapy. METHODS:This retrospective study involved 185 patients with locally advanced rectal cancer who underwent 30 Gy/10 f n...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-015-2226-6
更新日期:2015-06-01 00:00:00
abstract:OBJECTIVE:The objective of this study is to assess the value of metastatic lymph node ratio (LNR) in predicting disease-free survival (DFS) in patients with stage III adenocarcinoma of the colon. MATERIALS AND METHODS:From 1995 to 2003 inclusively, a total of 624 patients featuring stage III adenocarcinoma of the colo...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-009-0738-7
更新日期:2009-11-01 00:00:00
abstract:PURPOSE:The diagnosis of irritable bowel syndrome is symptom based, and colonoscopy is the most direct way to rule out organic colonic diseases. It is controversial on the necessity of colonoscopy for patients with suspected irritable bowel syndrome and lacking alarm features. This study was designed to verify the orga...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-011-1163-2
更新日期:2011-07-01 00:00:00
abstract:INTRODUCTION:The transplantation of neural crest derived stem cells (NCSC) is a potent alternative for the treatment of Hirschsprung's disease (HSCR). Cells to be transplanted should find an appropriate microenvironment to survive and differentiate. Influences of HSCR-smooth-muscle-protein extracts upon isolated myente...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-012-1411-0
更新日期:2012-06-01 00:00:00
abstract:BACKGROUND:Ulcerative colitis (UC) is an intractable colonic disease, and it shows several endoscopic findings. Recently, it was reported that the expression level of mucosal tumor necrosis factor alpha (TNF-α) was useful for predicting patient response to infliximab. However, no data regarding the value of endoscopic ...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-013-1654-4
更新日期:2013-08-01 00:00:00
abstract:AIM:To evaluate the reason for failure of STARR (stapled transanal rectal resection) operation for obstructed defecation. METHODS:A retrospective study (June 2012-December 2017) was performed using a prospectively maintained database of patients who underwent STARR operation for ODS (obstructed defecation syndrome), c...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-019-03263-9
更新日期:2019-05-01 00:00:00
abstract::Forty-five patients with soiling but without faecal incontinence were evaluated by means of anorectal function investigations (anal manometry, rectal capacity and saline infusion test). The causes of soiling and the effect of treatment on both soiling and anorectal function were studied. The results were compared with...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/BF01648548
更新日期:1989-01-01 00:00:00
abstract:OBJECTIVES:The aim of this study was to evaluate prospectively the functional outcome of posterior sagittal rectopexy with prolene mesh for rectal prolapse in young adults. PATIENTS AND METHODS:The study was carried out on 32 patients, 21 were males (65.63%) presented with complete rectal prolapse with a mean age of 3...
journal_title:International journal of colorectal disease
pub_type: 临床试验,杂志文章
doi:10.1007/s00384-010-0931-8
更新日期:2010-07-01 00:00:00
abstract:INTRODUCTION:Conventional haemorrhoidectomy (CH) is well known to cause significant post-operative pain and delayed return to daily activities. Both surgical wounds and sphincterial apparatus spasms are likely responsible for the pain. In this study, we evaluated the role of glyceryl trinitrate ointment (GTN) in reduci...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00384-012-1544-1
更新日期:2013-03-01 00:00:00
abstract:PURPOSE:The relationship between emergency colon cancer resection and long-term oncological outcomes is not well understood. Our objective was to characterize the impact of emergency resection for colon cancer on disease-free and overall patient survival. METHODS:Data on patients undergoing resection for colon cancer ...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-018-3109-4
更新日期:2018-11-01 00:00:00
abstract:PURPOSE:There is strong evidence supporting the importance of the volume-outcome relationship with respect to lung and pancreatic cancers. This relationship for rectal cancer surgery however remains unclear. We review the currently available literature to assess the evidence base for volume outcome in relation to recta...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,评审
doi:10.1007/s00384-010-1019-1
更新日期:2010-12-01 00:00:00
abstract:BACKGROUND AND AIMS:Colonic diverticulitis shows a high recurrence rate, but the role of faecal markers in predicting recurrence is unknown. The aim of this study was to investigate the role of faecal calprotectin (FC) in predicting recurrence of diverticulitis. PATIENTS/METHODS:A prospective cohort study was performe...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-014-1884-0
更新日期:2014-08-01 00:00:00
abstract:BACKGROUND AND AIMS:Despite the excellent results published on circular stapled mucosectomy (CSM), there is still some concern about the application of PPH-33 in the advanced haemorrhoidal disease, where a major prolapse may lead to insufficient resection and ensuing early recurrence. This study is aimed at comparing t...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00384-004-0720-3
更新日期:2006-01-01 00:00:00
abstract:BACKGROUND AND AIMS:Inpatients developing upper gastrointestinal (GI) haemorrhage are at increased risk of death. This study was performed to elucidate differences in inpatients and outpatients. PATIENTS/METHODS:Three hundred and sixty-two patients who needed esophagogastroduodenoscopy for upper GI bleeding were ident...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-004-0642-0
更新日期:2005-07-01 00:00:00
abstract::Previous studies have suggested that patients with familial adenomatous polyposis (FAP) have increased faecal excretion of cholesterol but a reduction in cholesterol metabolites. It was consequently proposed that the degree of faecal cholesterol degradation could be used as a means of diagnosis. Developments in the ex...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/BF00290303
更新日期:1993-12-01 00:00:00
abstract:BACKGROUND:Currently, many surgeons place a prophylactic drain in the abdominal or pelvic cavity after colorectal anastomosis as a conventional treatment. However, some trials have demonstrated that this procedure may not be beneficial to the patients. OBJECTIVE:To determine whether prophylactic placement of a drain i...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,meta分析
doi:10.1007/s00384-016-2509-6
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND/AIM:In order to define future chemoprevention strategies for adenomas or carcinomas in the pouch of patients with familial adenomatous polyposis (FAP), a 4-weeks intervention with (1) sulindac, (2) inulin/VSL#3, and (3) sulindac/inulin/VSL#3 was performed on 17 patients with FAP in a single center interventi...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,随机对照试验
doi:10.1007/s00384-010-1127-y
更新日期:2011-05-01 00:00:00
abstract:PURPOSE:Our previous study reported that carcinoembryonic antigen (CEA) levels in peritoneal fluid were significantly correlated with the prevalence of peritoneal carcinomatosis (PC) in colorectal cancer (CRC). The purpose of this study was a long-term follow up of the author's previous study, as well as the identifica...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-019-03448-2
更新日期:2020-01-01 00:00:00
abstract:OBJECTIVE:The objective of this study is to identify rates and factors associated with permanent diversion following restorative proctosigmoidectomy for rectal cancer when complicated by an anastomotic leak. DESIGN:This study is a retrospective review. SETTING:The setting of this study is a tertiary referral hospital...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-012-1423-9
更新日期:2012-10-01 00:00:00
abstract:PURPOSE:Hartmann's procedure for rectal cancer patients is increasingly performed but few studies have reported the postoperative outcome. The purpose was to report postoperative complications and analyse risk factors in rectal cancer patients operated with Hartmann's procedure. To describe the selection and postoperat...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-014-2069-6
更新日期:2015-02-01 00:00:00
abstract:PURPOSE:To analyze the results of abdominoperineal excisions (APE) for locally advanced rectal cancer at our institution before and after the adoption of extralevator abdominoperineal excision (ELAPE) with a special reference to long-term survival. METHODS:A retrospective cohort study conducted in a tertiary referral ...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-018-2977-y
更新日期:2018-04-01 00:00:00
abstract:PURPOSE:Large bowel obstruction and megacolon formation secondary to complicated diverticulitis is rare. METHODS:We present a case of an 84-year-old woman surviving large bowel obstruction and mega-megacolon formation secondary to complicated diverticulitis, with an impressive presentation of abdominal distention. RE...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-020-03642-7
更新日期:2020-08-01 00:00:00
abstract::A new case of benign lymphoid polyposis of the colon in an adult is presented. Relatively frequent in childhood, this disease remains exceptional in adults. The main concern is differentiation from adenomatous polyposis; the differential diagnosis can be particularly difficult for complex forms of polyposis with coexi...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/BF00341239
更新日期:1991-08-01 00:00:00
abstract:PURPOSE:This study was designed to examine the impact of an omentoplasty and its quality on pelviperineal morbidity after abdominoperineal resection (APR) for rectal cancer. METHODS:This was a retrospective single-centre study of consecutive patients undergoing APR for primary or recurrent rectal cancer between 2000 a...
journal_title:International journal of colorectal disease
pub_type: 杂志文章
doi:10.1007/s00384-019-03412-0
更新日期:2019-11-01 00:00:00
abstract:BACKGROUND:Surgical site infection (SSI) continues to be a challenge in colorectal surgery. Over the years, various modalities have been used in an attempt to reduce SSI risk in elective colorectal surgery, which include mechanical bowel preparation before surgery, oral antibiotics and intravenous antibiotic prophylaxi...
journal_title:International journal of colorectal disease
pub_type: 杂志文章,meta分析,评审
doi:10.1007/s00384-016-2662-y
更新日期:2017-01-01 00:00:00