Short-term phytohaemagglutinin-activated mononuclear cells induce endothelial progenitor cells from cord blood CD34+ cells.

Abstract:

:Endothelial progenitor cells (EPCs) were recently demonstrated to exist in human cord blood. Phytohaemagglutinin (PHA), a potent mitogen for mononuclear cells was used to induce EPCs from unsorted cord blood mononuclear cells (CBMCs). Adherent cells in clusters appeared approximately 24 h after CBMCs were cultured in plain Roswell Park Memorial Institute media containing 10% fetal bovine serum (culture media) and PHA. Adherent cells were further propagated for 1 week in plain culture media. Flow cytometry and Di-I staining analyses showed that CD45-, CD34+, Flk-1+, CD31+ or VE-cadherin+ EPCs were induced and that they were mainly from the CD34+ cell compartment. When enriched CD34+ cells alone were stimulated with culture supernatant of the PHA-activated CBMCs, they neither proliferated readily nor induced EPCs. Because EPCs first appeared within the clustering cells that expressed high levels of fibronectin and vascular endothelial growth factor (VEGF), our data suggest that both cell-cell/cell-matrix interaction and the local VEGF action are important in the induction of EPCs. Thus, we demonstrate for the first time that EPCs are induced from human cord blood stem cell populations that interact with neighbouring PHA-activated CBMCs. This finding may have a significant implication in inflammatory cell-mediated vasculogenesis and angiogenesis in vivo.

journal_name

Br J Haematol

authors

Kang HJ,Kim SC,Kim YJ,Kim CW,Kim JG,Ahn HS,Park SI,Jung MH,Choi BC,Kimm K

doi

10.1046/j.1365-2141.2001.02694.x

subject

Has Abstract

pub_date

2001-06-01 00:00:00

pages

962-9

issue

4

eissn

0007-1048

issn

1365-2141

pii

bjh2694

journal_volume

113

pub_type

杂志文章
  • Multiplex PCR reaction for the detection and identification of immunoglobulin kappa deleting element rearrangements in B-lineage leukaemias.

    abstract::Immunoglobulin kappa (Igkappa) gene recombinations can be used - similarly to IgH rearrangements - as clonal markers in B-lineage leukaemias. Based on the extensive junctional diversity, these rearrangements represent valuable targets for the analysis of minimal residual disease (MRD). In order to provide a simple met...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.1999.01557.x

    authors: Stolz F,Panzer S,Panzer-Grümayer ER

    更新日期:1999-08-01 00:00:00

  • Stereological studies on red corpuscle size produce values different from those obtained using haematocrit- and model-based methods.

    abstract::The routine methods for estimating erythrocyte size (volume and surface area) are not generally unbiased. Using geometric models introduces a bias determined by the ways in which real red blood corpuscles depart in size and shape from the simplistic ideal. Employing haematocrit and red corpuscle count to estimate mean...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1994.tb04739.x

    authors: Mayhew TM,Mwamengele GL,Self TJ,Travers JP

    更新日期:1994-02-01 00:00:00

  • The addition of rituximab to anthracycline-based chemotherapy significantly improves outcome in 'Western' patients with intravascular large B-cell lymphoma.

    abstract::Some case reports and a Japanese series suggest benefit from the use of rituximab in patients with intravascular large B-cell lymphoma (IVL). Rituximab efficacy was evaluated in Western patients with IVL, comparing outcome of 10 patients treated with rituximab + chemotherapy (R-CT) and of 20 patients treated with chem...

    journal_title:British journal of haematology

    pub_type: 杂志文章,多中心研究

    doi:10.1111/j.1365-2141.2008.07338.x

    authors: Ferreri AJ,Dognini GP,Bairey O,Szomor A,Montalbán C,Horvath B,Demeter J,Uziel L,Soffietti R,Seymour JF,Ambrosetti A,Willemze R,Martelli M,Rossi G,Candoni A,De Renzo A,Doglioni C,Zucca E,Cavalli F,Ponzoni M,Interna

    更新日期:2008-10-01 00:00:00

  • Patients with chronic lymphocytic leukaemia and clonal deletion of both 17p13.1 and 11q22.3 have a very poor prognosis.

    abstract::Detection of a 17p13.1 deletion (loss of TP53) or 11q22.3 deletion (loss of ATM), by fluorescence in situ hybridization (FISH), in chronic lymphocytic leukaemia (CLL) patients is associated with a poorer prognosis. Because TP53 and ATM are integral to the TP53 pathway, we hypothesized that 17p13.1- (17p-) and 11q22.3-...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/bjh.12534

    authors: Greipp PT,Smoley SA,Viswanatha DS,Frederick LS,Rabe KG,Sharma RG,Slager SL,Van Dyke DL,Shanafelt TD,Tschumper RC,Zent CS

    更新日期:2013-11-01 00:00:00

  • Risk factors for red blood cell alloimmunization in the Recipient Epidemiology and Donor Evaluation Study (REDS-III) database.

    abstract::Despite the significance of red blood cell (RBC) alloimmunization, the lack of standardized registries in the US has prevented the completion of large studies. Data from 3·5 years of the Recipient Epidemiology and Donor Evaluation Study-III (REDS-III) recipient database, containing information from 12 hospitals, were ...

    journal_title:British journal of haematology

    pub_type: 杂志文章,多中心研究

    doi:10.1111/bjh.15182

    authors: Karafin MS,Westlake M,Hauser RG,Tormey CA,Norris PJ,Roubinian NH,Wu Y,Triulzi DJ,Kleinman S,Hendrickson JE,NHLBI Recipient Epidemiology and Donor Evaluation Study-III (REDS-III).

    更新日期:2018-06-01 00:00:00

  • Polycythaemia-inducing mutations in the erythropoietin receptor (EPOR): mechanism and function as elucidated by epidermal growth factor receptor-EPOR chimeras.

    abstract::Primary familial and congenital polycythaemia (PFCP) is a disease characterized by increased red blood cell mass, and can be associated with mutations in the intracellular region of the erythropoietin (EPO) receptor (EPOR). Here we explore the mechanisms by which EPOR mutations induce PFCP, using an experimental syste...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/bjh.12782

    authors: Gross M,Ben-Califa N,McMullin MF,Percy MJ,Bento C,Cario H,Minkov M,Neumann D

    更新日期:2014-05-01 00:00:00

  • WASP is involved in proliferation and differentiation of human haemopoietic progenitors in vitro.

    abstract::The Wiskott-Aldrich syndrome (WAS) is an X-linked recessive disorder characterized by thrombocytopenia, immunodeficiency and eczema. X-linked thrombocytopenia (XLT) is a mild form of WAS with isolated thrombocytopenia. Both phenotypes are caused by mutation of the Wiskott-Aldrich syndrome protein (WASP) gene. In this ...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.1999.01694.x

    authors: Kajiwara M,Nonoyama S,Eguchi M,Morio T,Imai K,Okawa H,Kaneko M,Sako M,Ohga S,Maeda M,Hibi S,Hashimito H,Shibuya A,Ochs HD,Nakahata T,Yata JI

    更新日期:1999-11-01 00:00:00

  • Improved intensive care unit survival for critically ill allogeneic haematopoietic stem cell transplant recipients following reduced intensity conditioning.

    abstract::The use of allogeneic haematopoietic stem cell transplantation (Allo-HSCT) is a standard treatment option for many patients with haematological malignancies. Historically, patients requiring intensive care unit (ICU) admission for transplant-related toxicities have fared extremely poorly, with high ICU mortality rates...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/bjh.12294

    authors: Townsend WM,Holroyd A,Pearce R,Mackinnon S,Naik P,Goldstone AH,Linch DC,Peggs KS,Thomson KJ,Singer M,Howell DC,Morris EC

    更新日期:2013-05-01 00:00:00

  • Platelets derived from fresh and cold-stored whole blood participate in clot formation in rats with acute traumatic coagulopathy.

    abstract::The in vitro haemostatic functions of fresh whole blood (FWB) are well preserved after cold storage. This study aimed to determine whether platelets derived from FWB and stored whole blood (SWB) contribute to clot formation in tissue injury after transfusion into coagulopathic rats with polytrauma/haemorrhage (T/H). T...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/bjh.14999

    authors: Wu X,Darlington DN,Montgomery RK,Liu B,Keesee JD,Scherer MR,Benov A,Chen J,Cap AP

    更新日期:2017-12-01 00:00:00

  • Influence of karyotype on outcome of allogeneic bone marrow transplantation for adults with precursor B-lineage acute lymphoblastic leukaemia in first or second remission.

    abstract::The prognostic relevance of karyotype has been established in adult acute lymphoblastic leukaemia (ALL) patients treated with chemotherapy but not definitively evaluated in an allogeneic bone marrow transplantation (BMT) setting. To determine the factors affecting the outcome of allogeneic BMT for adults with precurso...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.2002.03403.x

    authors: Lee S,Kim DW,Kim YJ,Park YH,Min CK,Lee JW,Min WS,Kim CC

    更新日期:2002-04-01 00:00:00

  • The significance of TP53 in lymphoid malignancies: mutation prevalence, regulation, prognostic impact and potential as a therapeutic target.

    abstract::The tumour suppressor TP53 (previously termed p53) mediates a pathway that is considered to be one of the most important mechanisms in the maintenance of genomic stability. The function of TP53 can be abrogated by genomic deletion, mutation, or deregulation of upstream and downstream participants in the TP53 pathway. ...

    journal_title:British journal of haematology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2141.2009.07739.x

    authors: Cheung KJ,Horsman DE,Gascoyne RD

    更新日期:2009-08-01 00:00:00

  • Analysis of mutations at the DNA repair genes in acute childhood leukaemia.

    abstract::Deficiency in DNA repair capability is considered to be responsible for oncogenesis. Hereditary and sporadic cancers in various tissues have been reported to have mutations at the DNA repair genes. In this study we analysed two excision repair genes (ERCC1 and XPCC) and two mismatch repair genes (hMSH2 and hMTH1) in t...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.1998.00973.x

    authors: Lin YW,Kubota M,Koishi S,Sawada M,Usami I,Watanabe K,Akiyama Y

    更新日期:1998-11-01 00:00:00

  • Rh immunization facilitated by passively-administered anti-Rh?

    abstract::The response to 0.8 ml of D-positive (ccDEE) red cells injected i.v., together with 1 microgram anti-D i.m., was studied in 13 D-negative male volunteers (test subjects); the red cells were labelled with 51Cr and were found to have a mean survival time of 24 d, after correction for Cr elution. Within the following 5 m...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1983.tb01997.x

    authors: Contreras M,Mollison PL

    更新日期:1983-01-01 00:00:00

  • Plasma microparticles and vascular disorders.

    abstract::Microparticles are circulating, phospholipid rich, submicron particles released from the membranes of endothelial cells, platelets, leucocytes and erythrocytes. Investigation into their biological activity has revealed diverse actions in coagulation, cell signalling and cellular interactions. These actions are mediate...

    journal_title:British journal of haematology

    pub_type: 杂志文章,评审

    doi:10.1111/j.1365-2141.2007.06514.x

    authors: Lynch SF,Ludlam CA

    更新日期:2007-04-01 00:00:00

  • The effect of androstanes on granulopoiesis in vitro and in vivo.

    abstract::Bone marrow culture in a patient with aplastic anaemia responding to anabolic steroid (methandienone) therapy, showed an unusually high degree of growth in unstimulated cultures. Growth in unstimulated cultures is due to factors with colony stimulating activity (CSA) released by monocyte macrophages in the bone marrow...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1977.tb00990.x

    authors: Francis GE,Berney JJ,Bateman SM,Hoffbrand AV

    更新日期:1977-08-01 00:00:00

  • Neuro-meningeal relapse in anaplastic large-cell lymphoma: incidence, risk factors and prognosis - a report from the European intergroup for childhood non-Hodgkin lymphoma.

    abstract::Relapses involving the central nervous system (CNS) are rare in children and adolescents with ALK+ anaplastic large cell lymphoma (ALCL) treated with regimens including CNS prophylaxis. Early identification of patients at high-risk for CNS relapse would enable stratification and better adaptation of initial treatment ...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/bjh.16755

    authors: Del Baldo G,Abbas R,Woessmann W,Horibe K,Pillon M,Burke A,Beishuizen A,Rigaud C,Le Deley MC,Lamant L,Brugières L

    更新日期:2020-07-09 00:00:00

  • Erythropoiesis following bone marrow transplantation from donors heterozygous for beta-thalassaemia.

    abstract::This study shows a marked and protracted activation of HbF synthesis in homozygous beta.-thalassaemia patients transplanted from HLA identical siblings heterozygous for beta-thalassaemia, as compared to patients transplanted from normal donors. HbF synthesis in recipients was much higher in relation to the correspondi...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1989.tb04324.x

    authors: Galanello R,Barella S,Maccioni L,Paglietti E,Melis MA,Rosatelli MC,Argiolu F,Cao A

    更新日期:1989-08-01 00:00:00

  • Aminophospholipid translocase and phospholipid scramblase activities in sickle erythrocyte subpopulations.

    abstract::Phosphatidylserine (PS) externalization may contribute to Sickle Cell Disease (SCD) characteristics including thrombogenesis, endothelial adhesion and shortened red blood cell (RBC) lifespan. Aminophospholipid translocase (APLT) returns externalized PS to the inner membrane, and phospholipid scramblase (PLSCR) equilib...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.2009.07760.x

    authors: Barber LA,Palascak MB,Joiner CH,Franco RS

    更新日期:2009-08-01 00:00:00

  • An association between chronic neutrophilic leukaemia and multiple myeloma with a study of cobalamin-binding proteins.

    abstract::Two patients are described who had evidence of both multiple myeloma and chronic neutrophilic leukaemia at or near the time of presentation. Descriptions of five similar patients were found in the literature supporting an association between the two disorders. This association is further evidence of a link between mye...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1986.tb07507.x

    authors: Lewis MJ,Oelbaum MH,Coleman M,Allen S

    更新日期:1986-05-01 00:00:00

  • Increased concentration of the fast-acting plasminogen activator inhibitor in plasma associated with familial venous thrombosis.

    abstract::We have earlier demonstrated that in a family with a tendency to recurrent venous thrombosis the release of tissue plasminogen activator (t-PA) activity in blood after stimulation was abnormally low. This observation could be related either to an impaired release of t-PA into the blood stream or to a masking of the re...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1987.tb02261.x

    authors: Jørgensen M,Bonnevie-Nielsen V

    更新日期:1987-02-01 00:00:00

  • Single whole-leg compression ultrasound for exclusion of deep vein thrombosis in symptomatic ambulatory patients: a prospective observational cohort study.

    abstract::International guidance has recently recommended serial proximal compression ultrasound (CUS) as first line imaging for suspected deep vein thrombosis (DVT). Single whole-leg CUS is a routine alternative diagnostic strategy that can reduce repeated attendances and identify alternative pathology. We conducted a prospect...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/bjh.12642

    authors: Horner D,Hogg K,Body R,Jones S,Nash MJ,Mackway-Jones K

    更新日期:2014-02-01 00:00:00

  • Differentiation of patients with subtype IIb-like von Willebrand's disease by means of perfusion experiments with reconstituted blood.

    abstract::Four unrelated patients with a bleeding diathesis (bleeding time longer than 30 min), some spontaneous platelet aggregation, thrombocytopenia and large platelets, had decreased levels of factor VIII-von Willebrand factor (FVIII-VWF) related properties and impaired platelet adherence to human artery subendothelium. The...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1985.tb07333.x

    authors: Sakariassen KS,Nieuwenhuis HK,Sixma JJ

    更新日期:1985-03-01 00:00:00

  • A common polymorphism in the tumour necrosis factor-alpha gene associated with high TNF levels is not a risk factor for venous thromboembolism.

    abstract::The odds ratio of homozygosity or heterozygosity for the TNF2 polymorphism in 575 patients with venous thromboembolism compared to controls was found to be 1.0 (95% CI 0.4-2.1) and 1.1 (95% CI 0.8-1.4) respectively. Comparing subgroups of patients and controls with the factor V Leiden mutation the odds ratio for the T...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.1998.00729.x

    authors: Brown K,Luddington R,Baglin T

    更新日期:1998-06-01 00:00:00

  • SL-401 and SL-501, targeted therapeutics directed at the interleukin-3 receptor, inhibit the growth of leukaemic cells and stem cells in advanced phase chronic myeloid leukaemia.

    abstract::While imatinib and other tyrosine kinase inhibitors (TKIs) are highly efficacious in the treatment of chronic myeloid leukaemia (CML), some patients become refractory to these therapies. After confirming that interleukin-3 receptor (IL3R, CD123) is highly expressed on CD34(+) /CD38(-) BCR-ABL1(+) CML stem cells, we in...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/bjh.12978

    authors: Frolova O,Benito J,Brooks C,Wang RY,Korchin B,Rowinsky EK,Cortes J,Kantarjian H,Andreeff M,Frankel AE,Konopleva M

    更新日期:2014-09-01 00:00:00

  • Multiplex ligation-dependent probe amplification screening of isolated increased HbF levels revealed three cases of novel rearrangements/deletions in the beta-globin gene cluster.

    abstract::Investigations of naturally occurring mutations, such as the deletional thalassaemias and hereditary persistence of fetal haemoglobins (HPFHs), have brought many insights into human globin switching, but limited data have been reported so far. We selected 15 individuals with elevated fetal haemoglobin (HbF) levels (>5...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.2009.07927.x

    authors: Lee ST,Yoo EH,Kim JY,Kim JW,Ki CS

    更新日期:2010-01-01 00:00:00

  • The C807T/G873A polymorphism in the platelet glycoprotein Ia gene and the risk of acute coronary syndrome in the Italian population.

    abstract::Membrane glycoprotein (GP) Ia/IIa mediates platelet adhesion to collagen. The linked C807T/G873A polymorphisms in the GP Ia gene are correlated with a variable expression of the platelet surface receptor, the 807 TT/873 AA genotype being associated with a higher receptor density. Our study aimed to evaluate the possib...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.2001.02868.x

    authors: Casorelli I,De Stefano V,Leone AM,Chiusolo P,Burzotta F,Paciaroni K,Rossi E,Andreotti F,Leone G,Maseri A

    更新日期:2001-07-01 00:00:00

  • Modulation of gamma globin genes expression by histone deacetylase inhibitors: an in vitro study.

    abstract::Induction of fetal haemoglobin (HbF) is a promising therapeutic approach for the treatment of β-thalassaemia and sickle cell disease (SCD). Several pharmacological agents, such as hydroxycarbamide (HC) and butyrates, have been shown to induce the γ-globin genes (HBG1, HBG2). However, their therapeutic use is limited d...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/bjh.12814

    authors: Ronzoni L,Sonzogni L,Fossati G,Modena D,Trombetta E,Porretti L,Cappellini MD

    更新日期:2014-06-01 00:00:00

  • Simple in vivo bioassay for erythropoietin.

    abstract::A new method of in vivo bioassay for erythropoietin (EPO) is described. This method is based on the measurement of immature reticulocytes in EPO-treated mice using an automatic microcell counter, and is simpler and more precise than the existing methods of polycythaemic mouse assay and starved rat assay. Normal mice w...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.1991.tb08595.x

    authors: Kawamura A,Imai N,Kawaguchi T,Hayakawa T

    更新日期:1991-03-01 00:00:00

  • Serial studies of methylation of CDKN2B and CDKN2A in relapsed acute promyelocytic leukaemia treated with arsenic trioxide.

    abstract::Ninety consecutive patients with acute promyelocytic leukaemia were investigated for promoter methylation of CDKN2B (alias p15) and CDKN2A (alias p16) in disease relapse and progression. CDKN2B methylation was significantly more frequent at first relapse (30/36, 83%) than at presentation (48/77, 62%) (P=0.025), while ...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1111/j.1365-2141.2005.05818.x

    authors: Au WY,Fung AT,Ma ES,Chan CH,Wong KF,Chim CS,Liang RH,Kwong YL

    更新日期:2005-12-01 00:00:00

  • Antibody-mediated inhibition of the human alloimmune response to platelet transfusion in Hu-PBL-SCID mice.

    abstract::Severe combined immune deficient (SCID) mice were engrafted with human (Hu) peripheral blood lymphocytes (PBL) from a previously alloimmunized donor and transfused with HLA-mismatched platelets. We have previously shown this to be a useful model for platelet transfusion. These engrafted mice (Hu-PBL-SCID mice) produce...

    journal_title:British journal of haematology

    pub_type: 杂志文章

    doi:10.1046/j.1365-2141.1999.01237.x

    authors: Crow AR,Freedman J,Hannach B,Blanchette V,Lazarus AH

    更新日期:1999-03-01 00:00:00