Abstract:
:Calretinin (CR) is a neuronal EF-hand protein previously characterized as a calcium (micromolar affinity) binding protein. CR-containing neurons are spared in some neurodegenerative diseases, although it is as yet unconfirmed how CR plays an active role in this protection. Higher levels of some metal cations (e.g. copper and zinc) are associated with these diseases. At the same time, metals such as terbium (NMR and fluorescence) cadmium (NMR) and manganese (EPR) serve as useful calcium analogues in the study of EF-hand proteins. We survey the binding of the above-mentioned metal cations that might affect the structure and function of CR. Competitive 45Ca2+-overlay, competitive terbium fluorescence and intrinsic tryptophan fluorescence are used to detect the binding of metal cations to CR. Terbium and copper (half-maximal effect of 15 microM) bind to CR. Terbium has a similar or greater affinity for the calcium-binding sites of CR than calcium. Copper quenches the fluorescence of terbium-bound CR, and CR tryptophan residues and competes weakly for 45Ca2+-binding sites. Cadmium, magnesium, manganese and zinc bind less strongly (half-maximal effects above 0.1 mM). Therefore, only terbium appears to be a suitable analytical calcium analogue in further studies of CR. The principal conclusion of this work is that copper, in addition to calcium, might be a factor in the function of CR and a link between CR and neurodegenerative diseases.
journal_name
Acta Biochim Poljournal_title
Acta biochimica Polonicaauthors
Groves P,Palczewska Msubject
Has Abstractpub_date
2001-01-01 00:00:00pages
113-9issue
1eissn
0001-527Xissn
1734-154Xjournal_volume
48pub_type
杂志文章abstract::Infectious transcripts play a key role in the research on plant viruses at the molecular level. A number of cDNA clones covering the whole genome of the Polish isolate of potato leafroll virus were constructed. Four overlapping clones were selected and assembled using restriction sites. The full copy was positioned be...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract::The results of molecular dynamics simulation of fully hydrated dimyristoylphosphatidylcholine (DMPC) bilayer membrane in the liquid-crystalline phase are presented. They show that the probability of a gauche conformation varies periodically along the chain with only a slight increase towards the end of the chain. Howe...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1997-01-01 00:00:00
abstract::Uracil-DNA glycosylase of Archaeoglobus fulgidus (Afung) in cell extracts exhibited maximal activity around pH 6.2 as compared to pH 4.8 for the purified recombinant enzyme expressed in Escherichia coli. Native Afung thus seems to be adapted to the intracellular pH of A. fulgidus, determined to be 7.0±0.1. Both recomb...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::The use of nucleotides and their analogs in the pharmacological studies of nucleotide receptors (P2 class) should be preceded by detailed studies on their degradation connected with ecto-enzymes of a given cell type. In the present studies we have analyzed stability of some phosphorothioate and phosphonate analogs of ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:035004973
更新日期:2003-01-01 00:00:00
abstract::The map of candidate genes that can potentially affect physical fitness becomes larger every year, and they are associated with such aspects as respiratory and cardiovascular stability; body build and composition - especially muscle mass and strength; carbohydrate and lipid metabolism; response to training; and exerci...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2016_1422
更新日期:2018-01-01 00:00:00
abstract::Melanins form a diverse group of pigments synthesized in living organisms in the course of hydroxylation and polymerization of organic compounds. Melanin production is observed in all large taxa from both Pro- and Eukaryota. The basic functions of melanins are still a matter of controversy and speculation, even though...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2006-01-01 00:00:00
abstract::Bovine vitreous humour contains 0.6 +/- 0.04 mg of proteins per cm3. Three main protein fractions were obtained by CM-cellulose chromatography and their homogeneity was confirmed by isoelectric focusing and SDS-gel electrophoresis. The Mr and pl values of the separated fractions were: fraction I, 67,000 and 4.02; frac...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1989-01-01 00:00:00
abstract::The walls of human abdominal aortas and atherosclerosis-induced aneurysms contain similar amounts of collagen. The quantitative ratio between collagens of various types of this protein does not differ significantly either, whereas solubility of the collagen in aneurysmal wall and its susceptibility to the action of ED...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1995-01-01 00:00:00
abstract::The object of this paper is to review briefly the studies on the interaction of red blood cell membrane skeletal proteins and their non-erythroid analogues with lipids in model systems as well as in natural membranes. An important question to be addressed is the physiological significance and possible regulatory molec...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2000-01-01 00:00:00
abstract::This brief resume enumerates the multiple actions of melatonin as an antioxidant. This indoleamine is produced in the vertebrate pineal gland, the retina and possibly some other organs. Additionally, however, it is found in invertebrates, bacteria, unicellular organisms as well as in plants, all of which do not have a...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:0350041129
更新日期:2003-01-01 00:00:00
abstract::Human haptoglobin (Hp) of the 1-1 type incorporated one spin or fluorescence marker per molecule; the markers were found in the beta chain. Formation of the complex between spin-labelled Hp and haemoglobin or antibody caused conformational changes in the Hp molecular, evidenced by increased participation in the electr...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::All organisms are exposed to numerous stress factors, which include harmful xenobiotics. The diversity of these compounds is enormous, thus in the course of evolution diverse biological defense mechanisms at various levels of organization have developed. One of them engages an evolutionarily conserved family of transp...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2017_2299
更新日期:2017-01-01 00:00:00
abstract::In recent decades a significant raise in the incidence of myocardial infarction among young women has been recorded. It is presumed that, apart from the classical risk factors, other reasons exist for premature atherosclerosis in young women, related to the homeostasis of gonadal hormones. The aim of the study was to ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::Leukocyte adhesion to the vascular endothelium contributes to many immunological and inflammatory disorders. These processes have been shown to be mediated by bradykinin receptor type 2 (B2R) and dopamine receptor type 2 (D2R). In a previous study, we reported the formation of a B2R-D2R heterodimer, possibly altering ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2018_2641
更新日期:2018-01-01 00:00:00
abstract::New blocking group combinations for the machine-aided oligoribonucleotide synthesis on solid phase material have been developed and tested regarding their general application. An acetal function for 2'-OH protection offers a series of advantages in the synthetic approach but special conditions have to be fulfilled in ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:1996-01-01 00:00:00
abstract::Interaction of copper(II) with guanosine, 2'-deoxyguanosine, 1-methylguanosine, 7-methylguanosine and GMP was studied withe use of spectroscopic and magneto-chemical methods. The main site of copper(II) binding in guanosine is nitrogen N-7; participation of N-1 is not excluded. The involvement of carbonyl oxygen in co...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1978-01-01 00:00:00
abstract::Human STAU1 is one member of the family of double-stranded RNA (dsRNA)-binding proteins. It is thought to function in transporting mRNA, controlling translation and eliciting mRNA decay in neurons, and to function in infection of influenza virus and human immunodeficiency virus type 1 (HIV-1). Four transcripts coding ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2008-01-01 00:00:00
abstract::Cultivated carrot and its wild ancestor co-occur in most temperate regions of the world and can easily hybridize. The genetic basis of the process of domestication in carrot is not well understood. Recent results of an investigation on genetic diversity structure of cultivated and wild carrot and signatures for domest...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::In the present work, we have reviewed data showing that triiodothyronine and its nuclear receptors modify expression of different genes/proteins involved in cell cycle control beginning from growth factors (such as EGF and TGF-beta), to cell surface receptors (EGFR), as well as proteins acting at the cell membrane (Ra...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2006-01-01 00:00:00
abstract::Pathogen-specific acquired immunity in bacteria is mediated by the CRISPR (clustered regularly interspaced short palindromic repeats)-Cas systems. Thermus thermophilus strain HB8 contains CRISPR systems of several major subtypes (type I, IIIA and IIIB), and has become a widely studied model for CRISPR biology. We have...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2016_1261
更新日期:2016-01-01 00:00:00
abstract::A role of carotenoids as modulators of physical properties of model and biological membranes has been already postulated. However, there is a lack of information on the influence of these pigments on interactions between the lipids which form such membranes. This paper applies atomic force microscopy (AFM) in to study...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2012-01-01 00:00:00
abstract::The evidence on the crucial role of a family of calcium-dependent cysteine proteases called calpains in programmed cell death is rich and still growing. However, understanding of the mechanisms of their functions in apoptosis is not full yet. Calpains have been implicated in both physiological and pathological cell de...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章,评审
doi:
更新日期:2011-01-01 00:00:00
abstract::The protective action of quercetin against the pro-oxidant and apoptotic effect of etoposide was investigated in HL-60 cells with a high level of myeloperoxidase (MPO) activity and in cells treated with MPO inhibitor, 4-aminobenzoic acid hydrazide (ABAH). Quercetin significantly protected MPO-rich cells against the pr...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2014-01-01 00:00:00
abstract::Plasma membranes of rat platelets produced at normal platelet counts and during early recovery from immune-mediated thrombocytopenia were examined for the contents of carbohydrates, lipids and glycosphingolipids. Glucosylceramide, two monosialo-gangliosides and one disialo-ganglioside were found to be the major glycos...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1996-01-01 00:00:00
abstract::In plant cells, phospholipids are not only membrane components but also act as second messengers interacting with various proteins and regulating diverse cellular processes, including stress signal transduction. Here, we report studies on the effects of various phospholipids on the activity and expression of maize wou...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2011-01-01 00:00:00
abstract::Dansylated analogues of the potent and selective micro opioid peptide agonist [Dmt(1)]DALDA (H-Dmt-D-Arg-Phe-Lys-NH(2); Dmt = 2',6'-dimethyltyrosine) were prepared either by substitution of N(beta)-dansyl-alpha,beta-diaminopropionic acid or N(epsilon)-dansyllysine for Lys(4), or by attachment of a dansyl group to the ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:045101107
更新日期:2004-01-01 00:00:00
abstract::The base analogue 6-N-hydroxylaminopurine (HAP) is a potent mutagen in a variety of prokaryotic and eukaryotic organisms. Mutations in the yeast ham1 gene render the cells hypersensitive to the mutagenic effect of HAP. We have found that this gene has homologues in a variety of organisms from bacteria to man. We have ...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:1998-01-01 00:00:00
abstract:PURPOSE:To develop a novel intraarticular injection of diclofenac for the treatment of arthritis. METHOD:Diclofenac loaded nanoparticles were prepared by a nanoprecipitation technique using Eudragit L 100 as the polymer and polyvinyl alcohol as the surfactant. The nanoparticles were evaluated for particle size, zeta p...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2020_5401
更新日期:2021-01-22 00:00:00
abstract::The importance of nosocomial infections caused by coagulase-negative staphylococci is constantly growing. The threat primarily affects immunocompromised patients, the elderly and neonates, particularly after invasive surgery. The problem is fundamentally exacerbated by expanding antibacterial drug resistance. A case r...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:10.18388/abp.2015_1113
更新日期:2016-01-01 00:00:00
abstract::Cirrhosis leads to an inability of the liver to perform its biochemical functions. It can also lead to hepatocellular carcinoma in which, as we showed lately, arginase isoenzyme pattern changes. The present work presents our results on arginase isoenzymes and their possible role in liver cirrhosis. The study was perfo...
journal_title:Acta biochimica Polonica
pub_type: 杂志文章
doi:
更新日期:2009-01-01 00:00:00