Abstract:
:Monoclonal antibodies have played an important role in studying the biochemistry of the HLA-Class I molecules. Some murine anti-HLA mAbs can identify configurations of HLA epitopes that have never been reported in human allosera. One of these configurations is identified by an IgM mAb designated as: BHA-1441. This antibody was produced using a lymphoblastoid cell line typed as: A*02, A*25; B*38, B*4402/4405; C*0501, C*07, BW4, as the immunogen. A lymphocytotoxicity test of this mAb over a panel of 109 frozen, 452 fresh and, later, 44 DNA typed T cells revealed its specificity as B53, 37, 51, 52, +/- 44. All of the antigens recognized by this mAb share the Bw4 motif at positions 81-83, except for the HLA-B37, which shares only 82L and 83R. Furthermore, while B37 and B44 cross-react due to the aspartic acid (D) substitution at position 156, the reactivity with B53, B5 (51,52), B37 and 60% of B44 cells, makes it unlikely that the target epitope could be due only to the primary amino-acid sequence. The antibody-binding site might involve changes in tertiary structure and peptides bound by the MHC. BHA-1441 is an interesting tool to study and type the HLA-B53 antigen and its cross-reactive epitopes.
journal_name
Hum Immunoljournal_title
Human immunologyauthors
Etessami S,Tarsitani C,Lias M,Broadous M,Conger N,Randol G,Delory M,Bahrami S,Lee JHdoi
10.1016/s0198-8859(01)00254-3subject
Has Abstractpub_date
2001-07-01 00:00:00pages
732-8issue
7eissn
0198-8859issn
1879-1166pii
S0198885901002543journal_volume
62pub_type
杂志文章相关文献
HUMAN IMMUNOLOGY文献大全abstract::HLA-DQ molecules were isolated from DRw9-homozygous and DR4-homozygous cell lines by using a monoclonal antibody HU-18, which recognizes class II molecules carrying the conventional DQw3 determinant. The partial N-terminal sequence analysis of the DQw3 molecules revealed that they have sequences homologous to those of...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(85)90061-8
更新日期:1985-09-01 00:00:00
abstract::The objective was to investigate whether phagocytes from healthy and septic newborns have a developmental deficiency in their capacity to recognize, phagocytize and generate hydrogen peroxide (H2O2) in response to Escherichia coli and Staphylococcus aureus. TLR expression and phagocytic ability of neutrophils and mono...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2016.07.230
更新日期:2016-10-01 00:00:00
abstract::An interesting class of immune responses is that in which an environmental agent modifies a self-protein. Heparin induced thrombocytopenia (HIT) is associated with an antibody response in which the immunogen is a self-protein, platelet factor 4 (PF4), modified by an external agent, heparin. We tested the hypothesis th...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(00)00242-1
更新日期:2001-02-01 00:00:00
abstract::The human major histocompatibility complex includes a group of non-classical HLA class I genes, HLA-E, -F and -G. While nearly all focus since the discovery of these class Ib molecules have been on basic biochemistry and molecular biology of HLA-G and HLA-E, as well as their expression patterns, functions in immune mo...
journal_title:Human immunology
pub_type: 杂志文章,评审
doi:10.1016/j.humimm.2019.09.006
更新日期:2020-04-01 00:00:00
abstract::Human leukocyte antigen-G (HLA-G) is an anti-inflammatory and immunosuppressive molecule that can modulate immune cell activation. The role of HLA-G in tuberculosis, an immune-mediated and chronic bacterial disease remains to be elucidated. We investigated the expression profile of soluble and membrane bound HLA-G in ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2016.01.004
更新日期:2016-09-01 00:00:00
abstract::The aim of this investigation was to evaluate the relationship between MHC alleles at the HLA-DRB1, DQB1 and TNFa microsatellite loci and levels of oral bacteria that play a role in the etiology of dental caries, and the DMFS index in 186 AA primparous women. The average age of the cohort was 20.8+/-3.7 years. The med...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(99)00088-9
更新日期:1999-10-01 00:00:00
abstract::Prevention of rejection and the induction of transplantation tolerance are two related but separable phenomena that must both be considered in the analysis of the response to a transplanted organ. It is frequently hard to separate these phenomena in assessing the outcome of clinical transplants, because patients are r...
journal_title:Human immunology
pub_type: 杂志文章,评审
doi:10.1016/0198-8859(96)00059-6
更新日期:1996-09-15 00:00:00
abstract::The 2',5'-oligoadenylate synthetase 1 (OAS1) is one of the major interferon-inducible proteins and a critical component of the host defense system against viral infection. A single nucleotide polymorphism (SNP), rs10774671, presumably responsible for alternate splicing of this gene, has frequently been associated with...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2012.11.011
更新日期:2013-03-01 00:00:00
abstract::Five techniques, the direct and the antiglobulin enhanced cytotoxicity assays, indirect immunofluorescence, the enzyme-linked immunosorbent assay, and the radioimmunoassay, were evaluated in a workshop to determine their utility in studies of the interactions of monoclonal antibodies with HLA antigens expressed on lym...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(82)90016-7
更新日期:1982-04-01 00:00:00
abstract::Comparison of human leukocyte antigen (HLA) frequencies in patients with hepatitis C virus (HCV)-associated hepatocellular carcinoma (HCC) and in patients with HCV-associated non-Hodgkin's lymphoma (NHL) has not been addressed previously. To this aim, we investigated the distribution of HLA class II alleles in two sel...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2004.08.183
更新日期:2004-11-01 00:00:00
abstract::The Human Leukocyte Antigen-G has immunomodulatory function and its expression has been associated with several diseases. In our study we analyzed HLA-G polymorphisms in order to evaluate their possible association with susceptibility to celiac disease development. A total of 420 celiac patients and 509 controls were ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2014.12.006
更新日期:2015-01-01 00:00:00
abstract::Using TCR-specific mAbs, we analyzed the TCR distribution in the CD4 and CD8 compartments of peripheral blood T cells from members of six families, including two which were informative for an HLA-B/DR crossover. TCR distribution in the CD4 compartment is influenced by MHC class II antigens as it was similar in the chi...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(94)90055-8
更新日期:1994-06-01 00:00:00
abstract::Conventional matching is based on numbers of alleles shared between donor and recipient. This approach, however, ignores the degree of relationship between alleles and haplotypes, and therefore the actual degree of difference. To address this problem, we have compared family members using a block matching technique wh...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(97)00231-0
更新日期:1998-01-01 00:00:00
abstract::The HER-2/neu protein is overexpressed in approximately 20% of human adenocarcinomas and is a defined tumor antigen in breast cancer. The purpose of this study was to evaluate the endogenous HER-2/neu specific antibody response in 57 patients with colorectal cancer. HER-2/neu specific antibodies, titer > or = 1:100, w...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(99)00003-8
更新日期:1999-06-01 00:00:00
abstract::During HLA-DQ oligotyping, the presence of a novel HLA-DQB1 allele was suggested by a pattern of probe hybridization that was inconsistent with the presence of any possible combination of two known alleles. The nucleotide sequence of the second exon of the HLA-DQB1 gene was determined, revealing a novel HLA-DQB1 seque...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(92)90075-x
更新日期:1992-03-01 00:00:00
abstract::The detrimental impact of preformed anti-HLA donor-specific antibodies (DSA) is well defined, contrarily to non-donor-specific antibodies (NDSA). We sought to evaluate their clinical impact in a cohort of 724 kidney graft recipients in whom anti-HLA antibodies were thoroughly screened and identified in pre-transplant ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2017.07.011
更新日期:2017-09-01 00:00:00
abstract::The T-helper 1 (Th1) immune reaction is most important in dendritic cell (DC)-based immunotherapy. Interleukin (IL)-18, a Th1-biasing cytokine, plays a pivotal role in inducing cytotoxic T lymphocyte (CTL) responses. In this study, we analyzed whether dendritic cells (DCs) from patients with hepatocellular carcinoma (...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2007.01.008
更新日期:2007-05-01 00:00:00
abstract::Identification of mycobacterial antigens that are recognized by CD4+ Th1 cells in HLA-nonrestricted manner or in association with multiple allelic products is required to develop universally effective vaccines against mycobacterial diseases. Our studies in this direction have shown that several recombinant mycobacteri...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(99)00137-8
更新日期:2000-02-01 00:00:00
abstract::Natural antigen processing and presentation of antigen is thought to be important for the generation of a broad functional repertoire of antigen-specific T cells. In this study, the T-cell repertoire to an immunodominant human leukocyte antigen A2 (HLA-A2) binding peptide epitope of HER-2/neu, p369-377, was examined i...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(02)00401-9
更新日期:2002-07-01 00:00:00
abstract::Two members of a unique class of natural antibodies have been identified in all of a large cohort of sera from clinically normal humans of broad age distribution. By means of a series of 10-12 mer peptides the epitope for each of those antibodies was characterized with regard to amino acid identity and conformation. S...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(97)00100-6
更新日期:1997-07-01 00:00:00
abstract::We previously investigated the characteristics of renal allograft infiltrating T-cell lines that were propagated from biopsy and nephrectomy specimens designated as IG-Bip and IG-Neph, respectively, or analogous line designated IG-T-eff, which was generated by co-culturing pretransplant recipient blood with irradiated...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(94)00077-4
更新日期:1995-02-01 00:00:00
abstract::The allelic diversity and associated human leukocyte antigen (HLA) disparity of 1775 bone marrow recipients and their unrelated donors, matched for six of six (1361/1775,77%), five of six (397/1775, 22%), or four of six (17/1775, 1%) HLA-A, -B, -DR antigens, were retrospectively evaluated. The comprehensive HLA analys...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2006.09.004
更新日期:2007-01-01 00:00:00
abstract::Graves disease (GD) is an autoimmune thyroid disease and is associated with human leukocyte antigen (HLA)-DR3 and DQA1*0501 in Caucasians. However, the association of HLA with GD is less clear-cut in East Asian populations. We analyzed HLA-DRB1 and -DQB1 associations with GD in 198 Korean patients compared with 200 he...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2005.03.001
更新日期:2005-06-01 00:00:00
abstract::An HLA-E polymorphism study by oligotyping and DNA sequencing was carried out in the Spanish population. As a result, a new HLA-E allele (E*01031) initially assigned by polymerase chain reaction oligotyping as E*0104 was found. This allele presents a synonymous change at codon 77 (AAT-->AAC; Asn) when compared with th...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/s0198-8859(97)00008-6
更新日期:1997-04-15 00:00:00
abstract::The reactivity of monoclonal antibodies (mAbs) R1, S1, and S5, shown previously to recognize polymorphic epitopes on HLA-DQ molecules, have been found to correlate with the presence of certain DQB1 alleles. mAb S5 reacts with cells expressing DQB1*0503, 0601, 0602, 0603, or 0604 alleles while R1 and S1 react with all ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(91)90009-x
更新日期:1991-06-01 00:00:00
abstract::The dendritic cell (DC) lineage encompasses a diverse population of cells with unique subtype-specific functions. In peripheral blood, four DC subsets have been identified based on their distinct expression of CD1c, CD141, CD16, and CD123, and these subpopulations exhibit functional properties in immune responses. How...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2010.07.008
更新日期:2010-10-01 00:00:00
abstract::The objective of this study was to investigate whether there is an association between IL1RN polymorphism and disease susceptibility for three age-related inflammatory conditions: polymyalgia rheumatica (PMR), giant cell arteritis (GCA), and elderly-onset rheumatoid arthritis (EORA). A tandem-repeat polymorphism withi...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2008.10.011
更新日期:2009-01-01 00:00:00
abstract::We have calculated six-locus high resolution HLA A∼C∼B∼DRB3/4/5∼DRB1∼DQB1 haplotype frequencies using all Be The Match(®) Registry volunteer donors typed by DNA methods at recruitment. Mixed resolution HLA typing data was inputted to a modified expectation-maximization (EM) algorithm in the form of genotype lists gene...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/j.humimm.2013.06.025
更新日期:2013-10-01 00:00:00
abstract::The purpose of this paper is to report a pedigree with hereditary hemochromatosis in which a recombination between the HLA-A and B loci occurred. Both the maternal and paternal HLA-A3, B7 haplotypes were carrying an allele for hemochromatosis. The A3, B12 haplotype of the proband was a recombinant of the maternal A3, ...
journal_title:Human immunology
pub_type: 杂志文章
doi:10.1016/0198-8859(80)90005-1
更新日期:1980-07-01 00:00:00
abstract::Cytomegalovirus (CMV) is a well-known cause of disease occurring after bone marrow transplantation (BMT). The manifestations of CMV range from asymptomatic infection, defined as active CMV replication in the blood in the absence of clinical manifestations or organ failure abnormalities, to CMV disease, characterized b...
journal_title:Human immunology
pub_type: 杂志文章,评审
doi:10.1016/j.humimm.2004.02.013
更新日期:2004-05-01 00:00:00