Abstract:
:The activity of phospholipase A2 (PLA2) is elevated in the intestinal epithelia of patients with inflammatory bowel disease (IBD). We recently reported that PLA2 mediates hydrolysis of phosphatidylcholine (PC) to lysophosphatidylcholine (L-PC) when both are applied to the apical surface of cultured EC monolayers, resulting in increased bacterial translocation (BT) and decreased transepithelial electrical resistance (TEER). Free fatty acids (FFA) are the other products of this reaction, however, their effect on Caco-2 cell permeability has not been reported. In addition to PC, other luminal phospholipids are present at the surface of the enterocyte. PLA2 may also mediate the hydrolysis of luminal phospholipids other than PC. The aim of this study was to examine the effects of phospholipids other than PC and common FFA on intestinal epithelial permeability and BT. Human Caco-2 enterocytes were grown to confluence on porous filters in the apical chamber of a two-chamber cell-culture system. Monolayer integrity and tight-junction permeability were measured as TEER. First, common FFA released by PC hydrolysis were determined using thin-layer chromatography (TLC). In separate experiments, monolayers were treated with phosphatidylethanolamine (PE), lysophosphatidylethanolamine (L-PE), or palmitoleic acid, oleic acids, linoleic acids, and arachidonic acid solubilized in solution with PC. The magnitude of BT was determined 2 h after treatment by adding Escherichia coli C25 to the apical chamber followed by quantitatively culturing basal-chamber samples. Statistical analysis was by the Kurosaki-Wallis test. TLC of PC samples incubated with PLA2 on the apical surface of Caco-2 monolayers demonstrated the production of palmitoleic acid, oleic acids, linoleic acids, and arachidonic acid. L-PE significantly decreased TEER compared to controls, but to a lesser degree than L-PC alone. L-PE had no effects on BT. Palmitoleic acid and oleic acid likewise significantly decreased TEER compared to controls, however, less than L-PC. All FFA tested had no effect on BT. Phospholipids applied to the apical surface of enterocytes, such as those found in vivo in mucus, can be hydrolyzed by the enzyme PLA2 resulting in lysophospholipid and FFA species that can alter enterocyte monolayer permeability. However, FFA and L-PL, other than L-PC, appear to have no effect to stimulate BT. This observation may have clinical implications in the pathogenesis and treatment strategies for IBD patients in whom enterocyte PLA2 activity has been shown to be elevated.
journal_name
Pediatr Surg Intjournal_title
Pediatric surgery internationalauthors
Sawai T,Drongowski RA,Lampman RW,Coran AG,Harmon CMdoi
10.1007/s003830100592subject
Has Abstractpub_date
2001-05-01 00:00:00pages
269-74issue
4eissn
0179-0358issn
1437-9813journal_volume
17pub_type
杂志文章abstract::We analyzed the causes and diagnoses, the treatment, short and long-term outcome of a consecutive series of 70 pediatric polytrauma patients. From 1989 to 1996, 70 children (aged 10 months to 16 years, mean 7.4 years) presented with multiple trauma. A follow-up investigation was performed 4.2 years (mean) after the ac...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-003-0954-0
更新日期:2003-08-01 00:00:00
abstract:PURPOSE:The need for clinically applicable skin substitutes continues to be a matter of fact. Hypothetically, a laboratory grown autologous skin analog with near normal architecture might be a suitable approach to yield both satisfactory functional and cosmetic long-term results. In this study, we explored the use of h...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-015-3808-7
更新日期:2016-01-01 00:00:00
abstract:BACKGROUND:Subclinical varicocele is a relative rare condition. The aim of this study is to identify the role of bioflavonoids in the management of subclinical varicocele. MATERIALS AND METHODS:We analyzed the medical charts of patients treated for subclinical varicocele with bioflavonoids between 1999 and 2004; inclu...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-010-2574-9
更新日期:2010-05-01 00:00:00
abstract:PURPOSE:Ultraviolet (UV) radiation adversely affects skin health at cellular and molecular levels. Hence, UV radiation can directly induce inflammatory responses in the dermis by inducing erythema, edema, inflammation, dermal fibroblasts alterations, and extracellular matrix modifications. METHODS:Human keratinocytes,...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4384-4
更新日期:2019-01-01 00:00:00
abstract::"Variants of Hirschsprung's disease" are conditions that clinically resemble Hirschsprung's disease (HD), despite the presence of ganglion cells in rectal suction biopsies. The diagnosis and management of these patients can be challenging. Specific histological, immunohistochemical and electron microscopic investigati...
journal_title:Pediatric surgery international
pub_type: 杂志文章,评审
doi:10.1007/s00383-013-3351-3
更新日期:2013-09-01 00:00:00
abstract::We have reviewed 233 patients with posterior urethral valves treated in a single center in Calcutta, India, over the last 20 years: 37 were neonates, 75 were between 1 and 12 months, 88 were between 1 and 5 years, and 33 were more than 5 years old when first seen. The clinical presentation and methods employed in diag...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000495
更新日期:2001-01-01 00:00:00
abstract::Acute abdomen is caused by a wide variety of etiologies, many of which require surgical intervention. Two boys were hospitalized for acute abdominal pain and low-grade fever. Physical examination revealed epigastric and right upper abdominal fullness, and laboratory studies showed elevated erythrocyte sedimentation ra...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-002-0942-9
更新日期:2003-11-01 00:00:00
abstract::Juvenile papillomatosis (JP) is a rare benign, proliferative breast tumor in children. We observed a 9-year-old girl with a large soft-tissue mass at the lower outer quadrant of the left breast. Ultrasonography showed an ill-defined, inhomogeneous mass with numerous small, hypoechoic areas. The tumor was completely ex...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000462
更新日期:2001-03-01 00:00:00
abstract::Gastrointestinal atresia is a major cause of bowel obstruction in the newborn. Experimental models and clinical observations have demonstrated the heterogeneous nature of its pathogenesis. A proportion is due to late intra-uterine vascular insults and some are genetic in nature. Epidemiological studies have found gast...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-008-2138-4
更新日期:2008-06-01 00:00:00
abstract::Nonoperative management is acceptable treatment for minor pancreatic injuries. However, management of major pancreatic duct injury in children remains controversial. We present our experience in treating isolated pancreatic duct injury. We describe the cases of three male patients treated for complete pancreatic duct ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-014-3570-2
更新日期:2014-09-01 00:00:00
abstract::A new instrument for suction rectal biopsy in infants suspected of having Hirschsprung's disease is described that can be completely dismantled, physically cleaned, lubricated, and heat-sterilised. These attributes are new and are absolutely critical today. The instrument has been used in 60 patients (ca. 180 biopsies...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF01194812
更新日期:1997-01-01 00:00:00
abstract:PURPOSE:The aim of this long-term follow-up study was to investigate the current self assessed physical health in a CDH birth cohort at a single center. METHODS:Between 1990 and 2009, 195 children born with CDH were treated at Astrid Lindgren Children's Hospital. The primary survival rate was 85 %, and in 2010, 78 % w...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3879-0
更新日期:2016-05-01 00:00:00
abstract::The most common cystic lesion recognized antenatally is multicystic dysplastic kidney (MCDK). Recently, conservative management without nephrectomy has been advocated. The purpose of this study was to report our experience in the conservative management of unilateral MCDK. Between 1989 and 1997, 20 children with MCDK ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000449
更新日期:2001-01-01 00:00:00
abstract:AIM:Although liver abscess (LA) is prevalent worldwide, especially in developing countries, there is a paucity of data regarding the protocol for management of LA in children. The aim of this study was to analyze the outcome of a protocol-based management of pediatric LA from a single institution. MATERIALS AND METHOD...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-4009-8
更新日期:2017-02-01 00:00:00
abstract:AIM:The aim of our study is to introduce a new objective method of perioperative evaluation of the size of diaphragmatic defect to enable comparison of results among various centres and methods used for diaphragmatic reconstruction. MATERIALS AND METHODS:Prospective observational study of neonates with congenital diap...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-012-3113-7
更新日期:2012-10-01 00:00:00
abstract::Liver transplantation (LT) for malignancy has had disappointing long-term results due to tumour recurrence. Ex-vivo dissection and auto-transplantation have had poor results when the tumor was obstructing bile ducts. Advances in liver surgery have made extensive liver resection safer, but cases of "unresectable" tumou...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000531
更新日期:2001-07-01 00:00:00
abstract::A thoracic mass was identified on a routine ultrasound (US) examination in the 30th week of gestation. A postnatal chest radiograph, US, and computed tomography demonstrated a well-delineated, solid, cystic tumor in the left lower lobe. The mass was excised and a diagnosis of pulmonary blastoma (PB) was made on pathol...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/BF00497817
更新日期:1996-06-01 00:00:00
abstract:PURPOSE:Bone morphogenetic proteins (BMPs) are a group of growth factors that are implicated in intestinal growth, morphogenesis, differentiation, and homeostasis. The role of the BMP signaling cascade in stimulation of cell proliferation after massive small bowel resection is unknown. The purpose of this study was to ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-015-3829-2
更新日期:2016-02-01 00:00:00
abstract:PURPOSE:The objective of this study was to characterize the clinical course and outcomes of children with pancreatic pseudocysts that were initially treated non-operatively or with percutaneous drainage. METHODS:A retrospective review of children with pancreatic pseudocysts over a 12-year period was completed. Categor...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-012-3236-x
更新日期:2013-03-01 00:00:00
abstract:PURPOSE:Pediatric surgeons at our institution are often asked by families about a theoretical increased risk of severe common upper respiratory infections in children status post lung resection. No data exist on this topic. We, therefore, aimed to examine the risk of severe respiratory infection in children after pulmo...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-020-04851-5
更新日期:2021-01-10 00:00:00
abstract::To determine the incidence of cystic fibrosis (CF) in neonates with intestinal obstruction (NIO) secondary to meconium ileus (MI), jejunoileal atresia (JA), meconium plug syndrome (MPS), volvulus (V), and meconium peritonitis (MP) and analyze the correlation of ultrasonographic (US) signs with CF in NIO with a prenata...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-002-0781-8
更新日期:2003-04-01 00:00:00
abstract:PURPOSE:Recognition of physical child abuse is imperative for ensuring children's safety. Screening tools (ST) may increase identification of physical abuse; however, the extent of their use is unknown. This study assessed use of STs for physical abuse in children's hospitals and determined attitudes regarding STs. ME...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-016-3916-z
更新日期:2016-08-01 00:00:00
abstract:PURPOSE:To identify potential metabolic biomarkers for distinguishing malignant and benign thyroid nodules in children and adolescents using a metabolomics approach. METHODS:A total of 96 consecutive patients (median age 14.29 ± 2.31 years, range 9-18 years) who underwent thyroidectomy and 40 healthy controls were enr...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-019-04584-0
更新日期:2020-02-01 00:00:00
abstract:PURPOSE:Congenital hyperinsulinism is a rare disease, and the newly developed 18 fluoro-L-dihydroxyphenylalanine-positron emission tomography (18F-DOPA PET) examination can detect hyperplastic lesions. Our purpose was to report the results of a nationwide survey on surgical treatment of congenital hyperinsulinism in Ja...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-018-4315-4
更新日期:2018-10-01 00:00:00
abstract::Fetal skin wounds heal scarlessly while adult wounds scar. Fetal wound healing occurs in a physiologically hypoxic environment whereas in adult wound healing, cells have to acutely adapt to hypoxia caused by locally impaired blood supply. We examined the expression of hypoxia-inducible factor 1 (HIF-1), a potent trans...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830050735
更新日期:2000-01-01 00:00:00
abstract:BACKGROUND:Inguinal hernia repair is the most frequently performed surgical procedure in infants and children. Especially in premature infants, prevalence reaches up to 30% in coincidence with high rates of incarceration during the first year of life. These infants carry an increased risk of complications due to genera...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-014-3590-y
更新日期:2014-10-01 00:00:00
abstract:BACKGROUND:For a definitive diagnosis of congenital solitary kidney, renal scintigraphy is suggested as being the gold standard of ruling out ectopic functioning renal tissue, possibly missed by ultrasound. The aim of our study was to test ultrasonography precision in comparison with renal scintigraphy on a larger coho...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-019-04478-1
更新日期:2019-06-01 00:00:00
abstract::Negative pressure wound therapy (NPWT) is an accepted form of treatment in managing complex wounds in adults and children. It is not as widely used in the neonatal population due to limited clinical experience. We describe the application of the RENASYS™ system (Smith and Nephew, UK) in delivering NPWT to four neonate...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-011-2868-6
更新日期:2011-08-01 00:00:00
abstract::Interstitial cells of Cajal (ICCs) have been reported to play the role of a pacemaker in regulating bowel motility. The relationship between neurons and ICCs, however, remains unclear. Hirschsprung's disease (HD) is an ideal model for investigating this relationship. The operated specimens obtained from 6 short and 3 ...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s00383-002-0931-z
更新日期:2003-06-01 00:00:00
abstract::We present the results of a 6-year review of appendicitis. In the event of diagnostic doubt, a policy of active observation was instituted. This review endorses the validity of such a policy, indicating that it does not expose patients to increased morbidity. Data were collected prospectively over a 6-year period on 1...
journal_title:Pediatric surgery international
pub_type: 杂志文章
doi:10.1007/s003830000483
更新日期:2001-03-01 00:00:00